Protein Overview: UTP20

General Information:
Name(s) found:	UTP20 / YBL004W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|4388558\|emb\|CAA84821.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|147744598\|sp\|P35194.3\|UTP20_YEAST RecName: Full=U3 small nucleolar RNA-associated protein 20; Short=U3 snoRNA-associated protein 20; AltName: Full=U three protein 20 [NCBI NR] gi\|14270688\|ref\|NP_009551.2\| Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA; Utp20p [Saccharomyces cerevisiae] [NCBI NR] U3 small nucleolar RNA-associated protein 20 OS=Saccharomyces cerevisiae GN=UTP20 PE=1 SV=3 [Swiss-Prot] UTP20 U3 snoRNP protein [mips] Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA [SGD] YBL004W, Chr II from 227598-235079 [yeast_orfs2] Chr II from 227598-235079 [yeastorf3.fasta] UTP20 SGDID:S0000100, Chr II from 227601-235082, Uncharacterized ORF [yeastflysequest.fasta] UTP20 SGDID:S0000100, Chr II from 227639-235120, Uncharacterized ORF [10112005cervSGD.fasta] Possible snoRNA-binding protein, based on computational analysis of large-scale protein-protein interaction data [Saccharomyces cerevisiae]gi\|4388558\|emb\|CAA84821.1\| unnamed protein product [Saccharomyces cerevisiae] [nrdb_11152004_Chlamydomonas] UTP20 SGDID:S000000100, Chr II from 227639-235120, Uncharacterized ORF, "Possible snoRNA-binding protein, based on computational analysis of large-scale protein-protein interaction data" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] UTP20 SGDID:S000000100, Chr II from 227639-235120, Verified ORF, "Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA" [yeast-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA; Utp20p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] U3 small nucleolar RNA-associated protein 20 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=UTP20 PE=1 SV=3 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] UTP20 SGDID:S000000100, Chr II from 227636-235117, Genome Release 64-1-1, Verified ORF, "Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA" [yeast-030211-contam.fasta] UTP20 SGDID:S000000100, Chr II from 227636-235117, Genome Release 64-2-1, Verified ORF, "Component of the small-subunit (SSU) processome; SSU processome is involved in the biogenesis of the 18S rRNA" [20141208_orf_trans.fasta] YBL004W SGDID:S000000100, Chr II from 227636-235117, Genome Release 64-1-1, Verified ORF, "Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	2493 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA] nuclear outer membrane [IMP][IPI] nucleolus [IDA ] small nucleolar ribonucleoprotein complex [IPI]
Biological Process:	endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [IMP] endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [IMP] ribosome biogenesis [IEA] endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [IMP] rRNA processing [IEA][RCA]
Molecular Function:	snoRNA binding [RCA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAKQRQTTKS SKRYRYSSFK ARIDDLKIEP ARNLEKRVHD YVESSHFLAS FDQWKEINLS  60
   61 AKFTEFAAEI EHDVQTLPQI LYHDKKIFNS LVSFINFHDE FSLQPLLDLL AQFCHDLGPD 120
  121 FLKFYEEAIK TLINLLDAAI EFESSNVFEW GFNCLAYIFK YLSKFLVKKL VLTCDLLIPL 180
  181 LSHSKEYLSR FSAEALSFLV RKCPVSNLRE FVRSVFEKLE GDDEQTNLYE GLLILFTESM 240
  241 TSTQETLHSK AKAIMSVLLH EALTKSSPER SVSLLSDIWM NISKYASIES LLPVYEVMYQ 300
  301 DFNDSLDATN IDRILKVLTT IVFSESGRKI PDWNKITILI ERIMSQSENC ASLSQDKVAF 360
  361 LFALFIRNSD VKTLTLFHQK LFNYALTNIS DCFLEFFQFA LRLSYERVFS FNGLKFLQLF 420
  421 LKKNWQSQGK KIALFFLEVD DKPELQKVRE VNFPEEFILS IRDFFVTAEI NDSNDLFEIY 480
  481 WRAIIFKYSK LQNTEIIIPL LERIFSTFAS PDNFTKDMVG TLLKIYRKED DASGNNLLKT 540
  541 ILDNYENYKE SLNFLRGWNK LVSNLHPSES LKGLMSHYPS LLLSLTDNFM LPDGKIRYET 600
  601 LELMKTLMIL QGMQVPDLLS SCMVIEEIPL TLQNARDLTI RIKNVGAEFG KTKTDKLVSS 660
  661 FFLKYLFGLL TVRFSPVWTG VFDTLPNVYT KDEALVWKLV LSFIKLPDEN QNLDYYQPLL 720
  721 EDGANKVLWD SSVVRLRDTI DTFSHIWSKY STQNTSIIST TIERRGNTTY PILIRNQALK 780
  781 VMLSIPQVAE NHFVDIAPFV YNDFKTYKDE EDMENERVIT GSWTEVDRNV FLKTLSKFKN 840
  841 IKNVYSATEL HDHLMVLLGS RNTDVQKLAL DALLAYKNPT LNKYRDNLKN LLDDTLFKDE 900
  901 ITTFLTENGS QSIKAEDEKV VMPYVLRIFF GRAQVPPTSG QKRSRKIAVI SVLPNFKKPY 960
  961 INDFLSLASE RLDYNYFFGN SHQINSSKAT LKTIRRMTGF VNIVNSTLSV LRTNFPLHTN1020
 1021 SVLQPLIYSI AMAYYVLDTE STEEVHLRKM ASNLRQQGLK CLSSVFEFVG NTFDWSTSME1080
 1081 DIYAVVVKPR ISHFSDENLQ QPSSLLRLFL YWAHNPSLYQ FLYYDEFATA TALMDTISNQ1140
 1141 HVKEAVIGPI IEAADSIIRN PVNDDHYVDL VTLICTSCLK ILPSLYVKLS DSNSISTFLN1200
 1201 LLVSITEMGF IQDDHVRSRL ISSLISILKG KLKKLQENDT QKILKILKLI VFNYNCSWSD1260
 1261 IEELYTTISS LFKTFDERNL RVSLTELFIE LGRKVPELES ISKLVADLNS YSSSRMHEYD1320
 1321 FPRILSTFKG LIEDGYKSYS ELEWLPLLFT FLHFINNKEE LALRTNASHA IMKFIDFINE1380
 1381 KPNLNEASKS ISMLKDILLP NIRIGLRDSL EEVQSEYVSV LSYMVKNTKY FTDFEDMAIL1440
 1441 LYNGDEEADF FTNVNHIQLH RRQRAIKRLG EHAHQLKDNS ISHYLIPMIE HYVFSDDERY1500
 1501 RNIGNETQIA IGGLAQHMSW NQYKALLRRY ISMLKTKPNQ MKQAVQLIVQ LSVPLRETLR1560
 1561 IVRDGAESKL TLSKFPSNLD EPSNFIKQEL YPTLSKILGT RDDETIIERM PIAEALVNIV1620
 1621 LGLTNDDITN FLPSILTNIC QVLRSKSEEL RDAVRVTLGK ISIILGAEYL VFVIKELMAT1680
 1681 LKRGSQIHVL SYTVHYILKS MHGVLKHSDL DTSSSMIVKI IMENIFGFAG EEKDSENYHT1740
 1741 KVKEIKSNKS YDAGEILASN ISLTEFGTLL SPVKALLMVR INLRNQNKLS ELLRRYLLGL1800
 1801 NHNSDSESES ILKFCHQLFQ ESEMSNSPQI PKKKVKDQVD EKEDFFLVNL ESKSYTINSN1860
 1861 SLLLNSTLQK FALDLLRNVI TRHRSFLTVS HLEGFIPFLR DSLLSENEGV VISTLRILIT1920
 1921 LIRLDFSDES SEIFKNCARK VLNIIKVSPS TSSELCQMGL KFLSAFIRHT DSTLKDTALS1980
 1981 YVLGRVLPDL NEPSRQGLAF NFLKALVSKH IMLPELYDIA DTTREIMVTN HSKEIRDVSR2040
 2041 SVYYQFLMEY DQSKGRLEKQ FKFMVDNLQY PTESGRQSVM ELINLIITKA NPALLSKLSS2100
 2101 SFFLALVNVS FNDDAPRCRE MASVLISTML PKLENKDLEI VEKYIAAWLK QVDNASFLNL2160
 2161 GLRTYKVYLK SIGFEHTIEL DELAIKRIRY ILSDTSVGSE HQWDLVYSAL NTFSSYMEAT2220
 2221 ESVYKHGFKD IWDGIITCLL YPHSWVRQSA ANLVHQLIAN KDKLEISLTN LEIQTIATRI2280
 2281 LHQLGAPSIP ENLANVSIKT LVNISILWKE QRTPFIMDVS KQTGEDLKYT TAIDYMVTRI2340
 2341 GGIIRSDEHR MDSFMSKKAC IQLLALLVQV LDEDEVIAEG EKILLPLYGY LETYYSRAVD2400
 2401 EEQEELRTLS NECLKILEDK LQVSDFTKIY TAVKQTVLER RKERRSKRAI LAVNAPQISA2460
 2461 DKKLRKHARS REKRKHEKDE NGYYQRRNKR KRA

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		BFR2 BMS1 BRX1 CBF5 CIC1 DIP2 ECM16 EMG1 ENP1 ENP2 HAS1 IMP3 IMP4 KRE33 KRR1 MPP10 MRP7 MRPL23 MRPL28 MRPL39 MRPL4 MRPL44 MRPL7 NAN1 NOC4 NOP1 NOP14 NOP4 NOP56 NOP6 PNO1 PWP2 RCL1 ROK1 ROM2 RPS13 RPS22B RPS7A RPS8B, RPS8A RRP12 RRP9 SOF1 UTP10 UTP13 UTP15 UTP20 UTP21 UTP22 UTP30 UTP4 UTP6 UTP7 UTP8
View Details	Krogan NJ, et al. (2006)		AHA1 BUD3 CNS1 CPR6 DIG2 DNM1 DSF1, YNR073C FRT2 GPA2 HOM6 HSC82 HSP104 HSP82 MBB1 MRPL50 PAM1 PPT1 QNS1 RMD8 RTS1 SMT3 STI1 STP4 TDH1 UTP20 YBL104C
View Details	Gavin AC, et al. (2002)		AAR2 ADE4 ADH1 ARX1 ASC1 ATG26 BDF2 BEM2 BFR2 BMS1 BRX1 BUD20 BUD21 BUD3 CBF5 CCM1 CDC33 CDC55 CFT2 CIC1 CKA1 CKA2 CKB1 CKB2 CLU1 CMS1 COP1 CRN1 CSL4 CYR1 CYS4 DBP10 DBP2 DBP8 DBP9 DCP2 DIA4 DIM1 DIP2 DIS3 DRS1 DYN1 EBP2 ECM16 ECM29 ECM5 EHD3 EMG1 ENP1 ENP2 ERB1 ERO1 ESF1 ESF2 FAP7 FIP1 FPR4 FUN12 FYV4 GAR1 GCN1 GEP3 GLC7 GLY1 HAS1 HAT1 HAT2 HCA4 HDA1 HEK2 HER1 HHF1, HHF2 HIF1 HIS3 HRR25 HSC82 HSL1 HSM3 HTA2 ICL1 IMG1 IMG2 IML1 IMP3 IMP4 INP2 IPI1 IPI3 KAP123 KRE33 KRR1 LCP5 LHP1 LIP2 LOC1 LRP1 LSG1 LTV1 MAC1 MAK21 MAK5 MDN1 MEC1 MHR1 MIP6 MIS1 MPP10 MRD1 MRH4 MRM1 MRP1 MRP10 MRP13 MRP17 MRP20 MRP21 MRP4 MRP49 MRP51 MRP7 MRPL1 MRPL10 MRPL13 MRPL16 MRPL17 MRPL19 MRPL20 MRPL23 MRPL24 MRPL25 MRPL27 MRPL28 MRPL3 MRPL35 MRPL36 MRPL39 MRPL4 MRPL44 MRPL51 MRPL6 MRPL7 MRPL8 MRPS16 MRPS17 MRPS18 MRPS28 MRPS5 MRPS8 MRPS9 MRT4 MSB1 MTR3 MVD1 NAB3 NAB6 NAM9 NAN1 NAP1 NHP2 NIP1 NIP7 NMD3 NMD5 NOB1 NOC2 NOC3 NOC4 NOG1 NOG2 NOP1 NOP12 NOP14 NOP15 NOP16 NOP2 NOP4 NOP56 NOP58 NOP6 NOP7 NRD1 NSA1 NSA2 NUG1 PAP1 PAP2 PET123 PFK1 PFS2 PNO1 POL2 PRE6 PRE9 PRO1 PRP4 PRP43 PRT1 PSD2 PSE1 PSH1 PTA1 PTI1 PUF6 PUS7 PWP2 RBG1 RCL1 REF2 REI1 RET2 RIO2 RIX1 RIX7 RLP24 RLP7 RML2 RNA14 ROK1 ROM2 RPA135 RPA190 RPC40 RPF1 RPF2 RPG1 RPN12 RPN3 RPN6 RPN8 RPN9 RPP2B RPT2 RPT3 RPT5 RPT6 RRP1 RRP12 RRP4 RRP40 RRP42 RRP43 RRP45 RRP46 RRP6 RRP7 RRP9 RSA3 RSA4 RSC3 RSM10 RSM19 RSM22 RSM23 RSM24 RSM25 RSM26 RSM27 RSM7 RTC6 RTG2 RTR1 SAM1 SBP1 SCL1 SDA1 SEC1 SEC21 SEC26 SEC27 SEC7 SEN54 SIN4 SKI2 SKI3 SKI6 SKI7 SLX9 SMB1 SNF2 SNF5 SNP1 SNU114 SNU13 SNU66 SOF1 SPB1 SPB4 SRO9 SRP1 SSF1 STM1 STO1 SUI3 SUP35 SUP45 SWI3 SYP1 TAF5 TAO3 TEL1 TIF1, TIF2 TIF4631 TIF4632 TIF6 TSC11 TSR1 TUB3 UBP10 URB1 USO1 UTP10 UTP11 UTP13 UTP15 UTP18 UTP20 UTP21 UTP22 UTP30 UTP4 UTP6 UTP7 UTP8 UTP9 VAC14 VPS5 VTH2 YDR115W YFR006W YGL036W YGR054W YIR035C YJL017W YML6 YMR310C YOR059C YPR077C YSH1 YTM1
View Details	Ho Y, et al. (2002)		ADH2 AIM21 ATP4 AVO1 BUD20 CBF5 CBP2 CCM1 CDC33 CIC1 COF1 CPR1 DBP7 EHD3 ENP2 GBP2 GCN1 GPI15 HAS1 HHF1, HHF2 HMO1 HTB1 HTB2 HYP2 IMD1 IMD3 ISA1 KRE33 KRI1 LOS1 LSM2 MAM33 MDH1 MGM101 MRPS16 MSS116 NOC2 NOP12 NOP58 NOP6 NPL3 OYE2 PET127 POL5 PRO1 PRP43 PUF6 PWP1 RLI1 RLP24 RRP12 RRP5 RRP9 RRT6 RVB1 SEC7 TEF4 TIF4631 TMA19 TRA1 TSR1 UBA1 UBC6 URB1 URB2 UTP10 UTP20 YFL006W YPP1 YPT1 YRA1 YTM1
View Details	Qiu et al. (2008)		BUD21 DIP2 ENP1 GAR1 IMP3 LCP5 NAN1 RRP5 RRP9 SOF1 UTP10 UTP11 UTP13 UTP15 UTP20 UTP4 UTP5 UTP6 UTP7 UTP8 UTP9

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#20 Asynchronous Prep1-TiO2 Phosphopeptide enrichment, PartB	Keck JM, et al. (2011)
View Run	MLP2	#24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	PRP16	UTP20	2	Unpublished Fields Lab Data
View Screen	PRR1	UTP20	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	UTP20	Huh WK, et al. (2003)
View Data	UTP20 and NOP56	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..351]	MSA	2.006916	View MSA. No confident structure predictions are available.
2	View Details	[352..982]	MSA	1.004883	View MSA. No confident structure predictions are available.
3	View Details	[983..1683]	deduced	N/A	No confident structure predictions are available.
4	View Details	[1684..2493]	MSA	2.008001	View MSA. No confident structure predictions are available.
5	View Details	[591..825]	deduced	N/A	No confident structure predictions are available.
6	View Details	[826..1037]	Pfam	79.283997	No description for PF07539.3 was found. No confident structure predictions are available.
7	View Details	[1038..1208]	deduced	N/A	No confident structure predictions are available.
8	View Details	[1209..1317]	deduced	N/A	No confident structure predictions are available.
9	View Details	[1318..2197]	FFAS03	2.65	No description for 2ie3A was found.
10	View Details	[2198..2351]	deduced	N/A	No confident structure predictions are available.
11	View Details	[2352..2493]	MSA	1.036996	View MSA. No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
RNA binding	3.36069436734718	bayes_pls_golite062009
binding	2.36800795249393	bayes_pls_golite062009
nucleic acid binding	1.59410102249793	bayes_pls_golite062009
structural molecule activity	1.3916221897201	bayes_pls_golite062009
protein binding	1.35895139229414	bayes_pls_golite062009
mRNA binding	1.35186555044783	bayes_pls_golite062009
transcription regulator activity	1.08645493064764	bayes_pls_golite062009
DNA binding	0.956143892328242	bayes_pls_golite062009
transcription factor activity	0.371244009721798	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2