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Protein Overview: POL2

General Information:
Name(s) found: POL2 / YNL262W [SGD]
Description(s) found:
Found 27 descriptions.
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  • Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 2222 amino acids

    Gene Ontology:
    Cellular Component: replication fork [IDALink to PubMed article]
    epsilon DNA polymerase complex [IDALink to PubMed article]
    Biological Process: lagging strand elongation [TASLink to PubMed article]
    leading strand elongation [TASLink to PubMed article]
    nucleotide-excision repair [TASLink to PubMed article]
    mismatch repair [TASLink to PubMed article]
    DNA synthesis involved in DNA repair [IMPLink to PubMed article]
    chromatin silencing at telomere [IMPLink to PubMed article]
    Molecular Function: nucleotide binding [IEA]
    nucleic acid binding [IEA]
    DNA binding [IEA]
    zinc ion binding [IEA]
    DNA-directed DNA polymerase activity [IEA][IMP]
    nucleotidyltransferase activity [IEA]
    metal ion binding [IEA]
    double-stranded DNA binding [IDA]
    transferase activity [IEA]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MMFGKKKNNG GSSTARYSAG NKYNTLSNNY ALSAQQLLNA SKIDDIDSMM GFERYVPPQY  60
   61 NGRFDAKDID QIPGRVGWLT NMHATLVSQE TLSSGSNGGG NSNDGERVTT NQGISGVDFY 120
  121 FLDEEGGSFK STVVYDPYFF IACNDESRVN DVEELVKKYL ESCLKSLQII RKEDLTMDNH 180
  181 LLGLQKTLIK LSFVNSNQLF EARKLLRPIL QDNANNNVQR NIYNVAANGS EKVDAKHLIE 240
  241 DIREYDVPYH VRVSIDKDIR VGKWYKVTQQ GFIEDTRKIA FADPVVMAFD IETTKPPLKF 300
  301 PDSAVDQIMM ISYMIDGEGF LITNREIISE DIEDFEYTPK PEYPGFFTIF NENDEVALLQ 360
  361 RFFEHIRDVR PTVISTFNGD FFDWPFIHNR SKIHGLDMFD EIGFAPDAEG EYKSSYCSHM 420
  421 DCFRWVKRDS YLPQGSQGLK AVTQSKLGYN PIELDPELMT PYAFEKPQHL SEYSVSDAVA 480
  481 TYYLYMKYVH PFIFSLCTII PLNPDETLRK GTGTLCEMLL MVQAYQHNIL LPNKHTDPIE 540
  541 RFYDGHLLES ETYVGGHVES LEAGVFRSDL KNEFKIDPSA IDELLQELPE ALKFSVEVEN 600
  601 KSSVDKVTNF EEIKNQITQK LLELKENNIR NELPLIYHVD VASMYPNIMT TNRLQPDSIK 660
  661 AERDCASCDF NRPGKTCARK LKWAWRGEFF PSKMDEYNMI KRALQNETFP NKNKFSKKKV 720
  721 LTFDELSYAD QVIHIKKRLT EYSRKVYHRV KVSEIVEREA IVCQRENPFY VDTVKSFRDR 780
  781 RYEFKGLAKT WKGNLSKIDP SDKHARDEAK KMIVLYDSLQ LAHKVILNSF YGYVMRKGSR 840
  841 WYSMEMAGIT CLTGATIIQM ARALVERVGR PLELDTDGIW CILPKSFPET YFFTLENGKK 900
  901 LYLSYPCSML NYRVHQKFTN HQYQELKDPL NYIYETHSEN TIFFEVDGPY KAMILPSSKE 960
  961 EGKGIKKRYA VFNEDGSLAE LKGFELKRRG ELQLIKNFQS DIFKVFLEGD TLEGCYSAVA1020
 1021 SVCNRWLDVL DSHGLMLEDE DLVSLICENR SMSKTLKEYE GQKSTSITTA RRLGDFLGED1080
 1081 MVKDKGLQCK YIISSKPFNA PVTERAIPVA IFSADIPIKR SFLRRWTLDP SLEDLDIRTI1140
 1141 IDWGYYRERL GSAIQKIITI PAALQGVSNP VPRVEHPDWL KRKIATKEDK FKQTSLTKFF1200
 1201 SKTKNVPTMG KIKDIEDLFE PTVEEDNAKI KIARTTKKKA VSKRKRNQLT NEEDPLVLPS1260
 1261 EIPSMDEDYV GWLNYQKIKW KIQARDRKRR DQLFGNTNSS RERSALGSMI RKQAESYANS1320
 1321 TWEVLQYKDS GEPGVLEVFV TINGKVQNIT FHIPKTIYMK FKSQTMPLQK IKNCLIEKSS1380
 1381 ASLPNNPKTS NPAGGQLFKI TLPESVFLEE KENCTSIFND ENVLGVFEGT ITPHQRAIMD1440
 1441 LGASVTFRSK AMGALGKGIQ QGFEMKDLSM AENERYLSGF SMDIGYLLHF PTSIGYEFFS1500
 1501 LFKSWGDTIT ILVLKPSNQA QEINASSLGQ IYKQMFEKKK GKIETYSYLV DIKEDINFEF1560
 1561 VYFTDISKLY RRLSQETTKL KEERGLQFLL LLQSPFITKL LGTIRLLNQM PIVKLSLNEV1620
 1621 LLPQLNWQPT LLKKLVNHVL SSGSWISHLI KLSQYSNIPI CNLRLDSMDY IIDVLYARKL1680
 1681 KKENIVLWWN EKAPLPDHGG IQNDFDLNTS WIMNDSEFPK INNSGVYDNV VLDVGVDNLT1740
 1741 VNTILTSALI NDAEGSDLVN NNMGIDDKDA VINSPSEFVH DAFSNDALNV LRGMLKEWWD1800
 1801 EALKENSTAD LLVNSLASWV QNPNAKLFDG LLRYHVHNLT KKALLQLVNE FSALGSTIVY1860
 1861 ADRNQILIKT NKYSPENCYA YSQYMMKAVR TNPMFSYLDL NIKRYWDLLI WMDKFNFSGL1920
 1921 ACIEIEEKEN QDYTAVSQWQ LKKFLSPIYQ PEFEDWMMII LDSMLKTKQS YLKLNSGTQR1980
 1981 PTQIVNVKKQ DKEDSVENSL NGFSHLFSKP LMKRVKKLFK NQQEFILDPQ YEADYVIPVL2040
 2041 PGSHLNVKNP LLELVKSLCH VMLLSKSTIL EIRTLRKELL KIFELREFAK VAEFKDPSLS2100
 2101 LVVPDFLCEY CFFISDIDFC KAAPESIFSC VRCHKAFNQV LLQEHLIQKL RSDIESYLIQ2160
 2161 DLRCSRCHKV KRDYMSAHCP CAGAWEGTLP RESIVQKLNV FKQVAKYYGF DILLSCIADL2220
 2221 TI

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) DPB2 DPB3 DPB4 LHP1 POL2 STE50 TIF4631 TIF4632
    View Details (MIPS) Mewes HW, et al. (2004) CDC45 DPB11 DPB2 DPB3 MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 POL2 POL3 POL31 POL32
    View Details Krogan NJ, et al. (2006) DPB2 DPB3 DPB4 INM1 MBA1 MCM2 POL2 RPN4
    View Details Gavin AC, et al. (2006) DPB2 DPB4 POL2
    View Details Gavin AC, et al. (2002) AAR2 ASC1 BRX1 CBF5 CDC33 CIC1 CTF18 CTF8 DBP2 DBP9 DPB2 DPB4 DRS1 ELG1 ERB1 FUN12 GAR1 HAS1 HCA4 HEK2 HHF1, HHF2 IML1 ISW1 ISW2 KAP114 KRE33 KRR1 LHP1 MAK21 MDN1 MIS1 MOT1 MRP7 NAB3 NAB6 NAM7 NAP1 NHP2 NIP1 NOC2 NOG1 NOP1 NOP12 NOP2 NOP4 NOP56 NOP6 NOP7 NPL3 NPL6 NRD1 NRP1 NUG1 OLA1 POL2 PRP4 PUB1 PUF6 PUS7 RBG1 RET1 RFC1 RFC2 RFC3 RFC4 RFC5 RLP7 ROM2 RPA135 RPC82 RPO31 RRP12 SBP1 SGN1 SMB1 SMD3 SNF2 SNP1 SNU114 SNU66 SRO9 STH1 STM1 STO1 SUI3 SWI1 TIF1, TIF2 TIF4631 TIF4632 TOP1 TOP2 URB1 UTP10 UTP20 UTP21 VPS1 VPS13 YMR181C YMR310C YTM1
    View Details Ho Y, et al. (2002) CIC1 CKA1 CKA2 CKB1 CKB2 DBP10 ENP2 GDE1 HHF1, HHF2 HOT1 KRI1 MRPL1 NOC3 NOP12 NOP2 NOP7 PDI1 POB3 POL2 PUF6 PWP1 RRP14 RRP5 SFP1 SPT16 SSF1 TIF4631 TRL1 UTP22 VMR1 VPS73 WTM2 YRA1
    View Details Qiu et al. (2008) CDC45 DPB11 DPB2 DPB3 DPB4 HCS1 MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 POL1 POL12 POL2 POL3 POL30 POL31 POL32 PRI1 PRI2

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run CEP3 sample cbf3 from feb 2005 Sandall S, et all (2006)
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run MLP2 #29 Asynchronous Prep5-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #11 Mitotic Prep1-TiO2 enriched, new search criteria Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data POL2 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..100] deduced N/A No confident structure predictions are available.
    2 View Details [101..136]
    [246..534]    		 PSI-BLAST 274.0 Exonuclease domain of family B (archaeal and phage) DNA polymerases; T4-like DNA polymerase
    3 View Details [137..245]
    [535..555]
    [575..678]    		 PSI-BLAST 274.0 Exonuclease domain of family B (archaeal and phage) DNA polymerases; T4-like DNA polymerase
    4 View Details [556..574]
    [679..857]    		 PSI-BLAST 274.0 Exonuclease domain of family B (archaeal and phage) DNA polymerases; T4-like DNA polymerase
    5 View Details [858..1098] PSI-BLAST 13.0 No description for 1b1fR_ was found.
    6 View Details [1099..1232] MSA 2.037994 View MSA. No confident structure predictions are available.
    7 View Details [1233..1924] deduced N/A No confident structure predictions are available.
    8 View Details [1925..2222] MSA 3.018912 View MSA. No confident structure predictions are available.

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2 No functions predicted.
    3 No functions predicted.
    4 No functions predicted.
    5 No functions predicted.
    6
    Term Confidence Notes
  • DNA-directed DNA polymerase activity
    		•  10.6911520838834 bayes_pls_golite062009
  • DNA polymerase activity
    		•  10.645713605123 bayes_pls_golite062009
  • nucleotidyltransferase activity
    		•  8.39147983801981 bayes_pls_golite062009
  • DNA primase activity
    		•  6.94341770434315 bayes_pls_golite062009
  • transferase activity, transferring phosphorus-containing groups
    		•  4.49699229430126 bayes_pls_golite062009
  • endonuclease activity
    		•  4.05835176777658 bayes_pls_golite062009
  • endodeoxyribonuclease activity
    		•  4.04079853250282 bayes_pls_golite062009
  • transferase activity
    		•  3.80228208941612 bayes_pls_golite062009
  • DNA binding
    		•  2.64619844447089 bayes_pls_golite062009
  • nucleic acid binding
    		•  2.6018293992859 bayes_pls_golite062009
  • deoxyribonuclease activity
    		•  2.53877834217178 bayes_pls_golite062009
  • RNA polymerase activity
    		•  2.39159655026329 bayes_pls_golite062009
  • DNA-directed RNA polymerase activity
    		•  2.39159655026329 bayes_pls_golite062009
  • transcription regulator activity
    		•  2.22832306273638 bayes_pls_golite062009
  • ribonuclease activity
    		•  2.21640837000075 bayes_pls_golite062009
  • binding
    		•  1.84871443682239 bayes_pls_golite062009
  • exonuclease activity
    		•  1.38951646742998 bayes_pls_golite062009
  • transcription factor activity
    		•  1.18792852183348 bayes_pls_golite062009
  • exoribonuclease activity
    		•  0.985835839433199 bayes_pls_golite062009
  • catalytic activity
    		•  0.985794797454925 bayes_pls_golite062009
  • exoribonuclease activity, producing 5'-phosphomonoesters
    		•  0.91438167081716 bayes_pls_golite062009
  • damaged DNA binding
    		•  0.903519456111979 bayes_pls_golite062009
  • mismatch repair complex binding
    		•  0.893434681603753 bayes_pls_golite062009
  • chromatin binding
    		•  0.891218870764102 bayes_pls_golite062009
  • 3'-5'-exoribonuclease activity
    		•  0.589620648556852 bayes_pls_golite062009
  • structure-specific DNA binding
    		•  0.431057665208185 bayes_pls_golite062009
  • 3'-5' exonuclease activity
    		•  0.427833022898625 bayes_pls_golite062009
  • MutLalpha complex binding
    		•  0.419053137349237 bayes_pls_golite062009
  • protein binding
    		•  0.366887851366715 bayes_pls_golite062009
    		• 0.300511724300705 bayes_pls_golite062009
  • DNA helicase activity
    		•  0.280008381637593 bayes_pls_golite062009
  • endoribonuclease activity, producing 5'-phosphomonoesters
    		•  0.244405458380391 bayes_pls_golite062009
  • general RNA polymerase II transcription factor activity
    		•  0.213302068277253 bayes_pls_golite062009
  • single-stranded DNA binding
    		•  0.170862363182338 bayes_pls_golite062009
  • endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters
    		•  0.152950127352174 bayes_pls_golite062009
  • nuclease activity
    		•  0.126991239633667 bayes_pls_golite062009
  • transcription activator activity
    		•  0.0924765766526223 bayes_pls_golite062009
  • single-stranded DNA specific exodeoxyribonuclease activity
    		•  0.024241485627603 bayes_pls_golite062009
    7 No functions predicted.
    8 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle