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Protein Overview: RPA135

General Information:
Name(s) found: RPA135 / YPR010C [SGD]
Description(s) found:
Found 29 descriptions.
SHOW ALL
  • RNA polymerase I second largest subunit A135 [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 1203 amino acids

    Gene Ontology:
    Cellular Component: DNA-directed RNA polymerase I complex [TASLink to PubMed article]
    Biological Process: transcription from RNA polymerase I promoter [TAS]
    ribosome biogenesis [RCALink to PubMed article]
    Molecular Function: DNA-directed RNA polymerase activity [TASLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSKVIKPPGQ ARTADFRTLE RESRFINPPK DKSAFPLLQE AVQPHIGSFN ALTEGPDGGL  60
   61 LNLGVKDIGE KVIFDGKPLN SEDEISNSGY LGNKLSVSVE QVSIAKPMSN DGVSSAVERK 120
  121 VYPSESRQRL TSYRGKLLLK LKWSVNNGEE NLFEVRDCGG LPVMLQSNRC HLNKMSPYEL 180
  181 VQHKEESDEI GGYFIVNGIE KLIRMLIVQR RNHPMAIIRP SFANRGASYS HYGIQIRSVR 240
  241 PDQTSQTNVL HYLNDGQVTF RFSWRKNEYL VPVVMILKAL CHTSDREIFD GIIGNDVKDS 300
  301 FLTDRLELLL RGFKKRYPHL QNRTQVLQYL GDKFRVVFQA SPDQSDLEVG QEVLDRIVLV 360
  361 HLGKDGSQDK FRMLLFMIRK LYSLVAGECS PDNPDATQHQ EVLLGGFLYG MILKEKIDEY 420
  421 LQNIIAQVRM DINRGMAINF KDKRYMSRVL MRVNENIGSK MQYFLSTGNL VSQSGLDLQQ 480
  481 VSGYTVVAEK INFYRFISHF RMVHRGSFFA QLKTTTVRKL LPESWGFLCP VHTPDGSPCG 540
  541 LLNHFAHKCR ISTQQSDVSR IPSILYSLGV APASHTFAAG PSLCCVQIDG KIIGWVSHEQ 600
  601 GKIIADTLRY WKVEGKTPGL PIDLEIGYVP PSTRGQYPGL YLFGGHSRML RPVRYLPLDK 660
  661 EDIVGPFEQV YMNIAVTPQE IQNNVHTHVE FTPTNILSIL ANLTPFSDFN QSPRNMYQCQ 720
  721 MGKQTMGTPG VALCHRSDNK LYRLQTGQTP IVKANLYDDY GMDNFPNGFN AVVAVISYTG 780
  781 YDMDDAMIIN KSADERGFGY GTMYKTEKVD LALNRNRGDP ITQHFGFGND EWPKEWLEKL 840
  841 DEDGLPYIGT YVEEGDPICA YFDDTLNKTK IKTYHSSEPA YIEEVNLIGD ESNKFQELQT 900
  901 VSIKYRIRRT PQIGDKFSSR HGQKGVCSRK WPTIDMPFSE TGIQPDIIIN PHAFPSRMTI 960
  961 GMFVESLAGK AGALHGIAQD STPWIFNEDD TPADYFGEQL AKAGYNYHGN EPMYSGATGE1020
 1021 ELRADIYVGV VYYQRLRHMV NDKFQVRSTG PVNSLTMQPV KGRKRHGGIR VGEMERDALI1080
 1081 GHGTSFLLQD RLLNSSDYTQ ASVCRECGSI LTTQQSVPRI GSISTVCCRR CSMRFEDAKK1140
 1141 LLTKSEDGEK IFIDDSQIWE DGQGNKFVGG NETTTVAIPF VLKYLDSELS AMGIRLRYNV1200
 1201 EPK

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) ARO1 CLU1 EFT2, EFT1 RET1 RPA12 RPA135 RPA14 RPA190 RPA34 RPA43 RPA49 RPB10 RPB2 RPB5 RPB8 RPC19 RPC40 RPC82 RPL13B RPO26 RPO31
    View Details (MIPS) Mewes HW, et al. (2004) RPA12 RPA135 RPA14 RPA190 RPA34 RPA43 RPA49 RPB10 RPB5 RPB8 RPC10 RPC19 RPC40 RPO26
    View Details Krogan NJ, et al. (2006) BDP1 CDC14 COY1 FAR7 MAF1 RET1 RPA12 RPA135 RPA14 RPA190 RPA34 RPA43 RPA49 RPC10 RPC11 RPC17 RPC19 RPC25 RPC31 RPC34 RPC37 RPC40 RPC53 RPC82 RPO31 YDL144C YIR024C
    View Details Gavin AC, et al. (2006) MET10 RPA135
    View Details Gavin AC, et al. (2002) ACC1 AMS1 APL2 APL4 APM1 APM2 APS1 ARO1 ARX1 ASC1 BDF2 BEM2 BMS1 BNI1 BRX1 BUD20 CCT8 CDC14 CDC48 CDC55 CIC1 CKB1 CLU1 CPA2 CRN1 CYR1 CYS4 DBP10 DBP9 DCP2 DHH1 DOA1 DPB2 DPB4 DRS1 DYN1 EBP2 ECM29 ECM5 ERB1 FBA1 FDH1 FPR4 GCN1 GCN20 GFA1 GLY1 GUS1 HAS1 HAT1 HAT2 HDA1 HER1 HHF1, HHF2 HIF1 HIS3 HSC82 HSM3 ICL1 IDH2 ILV1 IML1 INP2 IPI1 IPI3 ISW1 ISW2 KAP114 KAP123 KRE33 LHP1 LIP2 LOC1 LSG1 LYS12 MAC1 MAF1 MAK21 MAK5 MDN1 MEC1 MIP6 MIS1 MKT1 MOT1 MRPL23 MRPL3 MRPS28 MRT4 MSB1 NAB3 NAB6 NAP1 NIP1 NIP7 NMD3 NMD5 NOC2 NOC3 NOG1 NOG2 NOP1 NOP12 NOP15 NOP16 NOP2 NOP4 NOP56 NOP7 NPL4 NPL6 NRD1 NSA1 NSA2 NUG1 PDC1 PFK1 POL2 PRO1 PRT1 PSA1 PSD2 PSE1 PSH1 PUF6 RAD51 RAD52 RAI1 REG1 REI1 RET1 RFA1 RIX1 RIX7 RLP24 RLP7 ROM2 RPA12 RPA135 RPA190 RPA43 RPA49 RPB10 RPB2 RPB5 RPB8 RPC11 RPC17 RPC19 RPC25 RPC31 RPC34 RPC37 RPC40 RPC53 RPC82 RPF1 RPF2 RPG1 RPN10 RPN11 RPN12 RPN3 RPN6 RPN8 RPN9 RPO26 RPO31 RPP2B RPT2 RPT3 RPT5 RPT6 RRP1 RRP12 RSA3 RSA4 RSC3 RSC8 RTR1 RVB2 SAM1 SDA1 SEC27 SEC7 SEN54 SHP1 SKI2 SMC3 SNU13 SPB1 SPB4 SRO7 SSF1 STH1 SUI3 SUP35 SUP45 TAO3 TCB1 TEL1 TIF1, TIF2 TIF6 TOP1 TSA1 TSC11 TUB3 UFD1 URA7 URB1 USO1 UTP10 UTP20 UTP22 VAC14 VAS1 VPS1 VPS5 VTH2 YDR049W YFR006W YGL036W YHB1 YJL017W YNK1 YOL098C YPR077C YTM1
    View Details Ho Y, et al. (2002) ACC1 ACH1 ADH2 ADR1 AHA1 AIM13 ARF1 ARF2 ARO4 ARP2 ARP7 AVO1 BEM3 CCT2 CDC55 CDC60 CYS4 DIG1 DIG2 DOP1 ECM29 FET4 GCD11 GFA1 GND1 GPG1 GUS1 HAS1 HTA2 HXT6 IDH2 ILS1 ISA2 KAP122 KRI1 KSS1 MCM4 MDH1 MET18 MKT1 MSE1 NAP1 NGL2 NPA3 PHO84 PIM1 PMA1 PPE1 PPH22 PYC1 PYC2 RET1 RGC1 RPA12 RPA135 RPA190 RPA43 RPA49 RPB11 RPB5 RPC19 RPC25 RPC34 RPC40 RPC82 RPN10 RPN3 RPN6 RPO26 RPO31 RTS1 RTS3 RVB1 RVS167 SAN1 SEC27 SEN1 SMC4 SRP1 SRY1 STE11 STE12 STE7 TAP42 TBS1 TEC1 TFP1 TPD3 TRP5 TSA2 TSC11 UBI4 URE2 VMA4 YDR239C YER138C YFL042C YHR033W YHR112C YPL113C
    View Details Qiu et al. (2008) RPA12 RPA135 RPA14 RPA190 RPA34 RPA43 RPA49 RPB10 RPB3 RPB5 RPB7 RPB8 RPC10 RPC19 RPC25 RPC34 RPC40 RPO21 RPO26 RPO31

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run RPA135 his-HA tag on RPA135 Schneider, DA, et al. (2006)
    View Run RPA135 his-HA tag on RPA135 Schneider, DA, et al. (2006)
    View Run RPA135 his-HA tag on RPA135 Schneider, DA, et al. (2006)
    View Run RPA135 his-HA tag on RPA135 Schneider, DA, et al. (2006)
    View Run CEP3 sample cbf3 from feb 2005 Sandall S, et all (2006)
    View Run ASF1 3912: TAP-tagged, strain background includes hpc2(delta) Green EM, et al (2005)
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run ZDS1 Sample 2- cpn2 from june 2005 McCusker D, et al (2007)
    View Run ZDS1 Sample 3 - cpn + atp, from june 2005 McCusker D, et al (2007)
    View Run BOI1 Sample bob1 (2nd set) from october 2005 McCusker D, et al (2007)
    View Run MLP2 #29 Asynchronous Prep5-TiO2 Flowthrough Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data RPA135 Huh WK, et al. (2003)
    View Data RPA135 and NOP56 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..895] MSA 34.361906 View MSA. No confident structure predictions are available.
    2 View Details [896..977] deduced N/A No confident structure predictions are available.
    3 View Details [978..1071] MSA 3.231973 View MSA. No confident structure predictions are available.
    4 View Details [1072..1203] MSA 28.228997 View MSA. No confident structure predictions are available.

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • DNA-directed RNA polymerase activity
    		•  10.2021663255698 bayes_pls_golite062009
  • RNA polymerase activity
    		•  10.2021663255698 bayes_pls_golite062009
  • nucleotidyltransferase activity
    		•  7.6240729546188 bayes_pls_golite062009
  • general RNA polymerase II transcription factor activity
    		•  6.31198922553685 bayes_pls_golite062009
  • transferase activity, transferring phosphorus-containing groups
    		•  5.44374972993429 bayes_pls_golite062009
  • transferase activity
    		•  4.83218811360621 bayes_pls_golite062009
  • transcription regulator activity
    		•  2.34884279395902 bayes_pls_golite062009
  • DNA binding
    		•  2.02586687916169 bayes_pls_golite062009
  • nucleic acid binding
    		•  1.91447180328254 bayes_pls_golite062009
  • binding
    		•  1.53568146966362 bayes_pls_golite062009
  • RNA polymerase II transcription mediator activity
    		•  1.53259746846673 bayes_pls_golite062009
  • catalytic activity
    		•  1.36406839864424 bayes_pls_golite062009
  • RNA binding
    		•  1.12618950637766 bayes_pls_golite062009
  • RNA polymerase II transcription factor activity
    		•  0.847783043631028 bayes_pls_golite062009
  • protein binding
    		•  0.35192339064931 bayes_pls_golite062009
    2 No functions predicted.
    3 No functions predicted.
    4 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle