Bait Protein: | ASF1 |
Organism: | Saccharomyces cerevisiae |
Publication: | Green EM, Antczak AJ, Bailey AO, Franco AA, Wu KJ, Yates JR 3rd, Kaufman PD. Replication-independent histone deposition by the HIR complex and Asf1. Curr Biol. 2005 Nov 22;15(22):2044-9. |
Comments: | 3912: TAP-tagged, strain background includes hpc2(delta) |
Raw Data: |
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FILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
ASF1 | Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein t... | 78 | 1394 | 70.3% | 0.716 | 31603 |
[ |
RAD53 | Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autopho... | 90 | 362 | 63.9% | 0.0632 | 91962 |
[ |
SHM1 | Mitochondrial serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahy... | 8 | 8 | 26.1% | 0.00234 | 53686 |
[ |
GPM1 | Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycer... | 3 | 3 | 25.9% | 0.00174 | 27609 |
[ |
PDC1 | Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... | 9 | 16 | 20.8% | 0.00407 | 61495 |
[ |
ENO2 | Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... | 6 | 7 | 20.6% | 0.0023 | 46914 |
[ |
HHF1, HHF2 | Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two... | 3 | 7 | 18.4% | 0.00974 | 11368 |
[ |
PGK1 | 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phospha... | 4 | 4 | 14.4% | 0.00138 | 44738 |
[ |
YDJ1 | Protein chaperone involved in regulation of the HSP90 and HSP70 functions; involved in protein trans... | 2 | 2 | 13.4% | 7.01E-4 | 44671 |
[ |
ENO1 | Enolase I, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosph... | 4 | 5 | 12.8% | 0.00164 | 46816 |
[ |
IST1 | Protein with a positive role in the multivesicular body sorting pathway; functions and forms a compl... | 2 | 2 | 10.1% | 9.62E-4 | 34496 |
[ |
CYS4 | Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the ... | 2 | 2 | 8.5% | 5.66E-4 | 56022 |
[ |
SAM1 | S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom ... | 2 | 2 | 8.4% | 7.51E-4 | 41818 |
[ |
SAM2 | S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom ... | 2 | 2 | 8.3% | 7.47E-4 | 42256 |
[ |
CDC19 | Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... | 4 | 5 | 8.2% | 0.00143 | 54545 |
[ |
YHR054C | Putative protein of unknown function | 2 | 2 | 8.2% | 8.1E-4 | 41061 |
[ |
TEF1, TEF2 | Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... | 2 | 2 | 8.1% | 6.26E-4 | 50033 |
[ |
GLO4 | Mitochondrial glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and... | 2 | 2 | 7.4% | 0.00101 | 32339 |
[ |
PAP2 | Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP co... | 2 | 2 | 6.7% | 4.91E-4 | 66031 |
[ |
CYS3 | Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathw... | 2 | 2 | 6.6% | 7.28E-4 | 42542 |
[ |
ADH1 | Alcohol dehydrogenase, fermentative isozyme active as homo- or heterotetramers; required for the red... | 2 | 2 | 6.6% | 8.24E-4 | 36849 |
[ |
SPG5 | Protein required for survival at high temperature during stationary phase; not required for growth o... | 2 | 2 | 6.4% | 7.69E-4 | 42163 |
[ |
HSP60 | Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides... | 2 | 2 | 5.4% | 5.01E-4 | 60752 |
[ |
ADP1 | Putative ATP-dependent permease of the ABC transporter family of proteins | 2 | 3 | 4.9% | 4.1E-4 | 117231 |
[ |
MSC6 | Protein of unknown function; mutant is defective in directing meiotic recombination events to homolo... | 2 | 2 | 4.6% | 4.14E-4 | 79947 |
[ |
DSL1 | Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p a... | 3 | 4 | 4.2% | 7.61E-4 | 88072 |
[ |
PDC5 | Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruva... | 2 | 3 | 3.9% | 7.64E-4 | 61912 |
[ |
VPS34 | Phosphatidylinositol 3-kinase responsible for the synthesis of phosphatidylinositol 3-phosphate; for... | 2 | 2 | 3.8% | 3.28E-4 | 100921 |
[ |
PIB2 | Protein binding phosphatidylinositol 3-phosphate, involved in telomere-proximal repression of gene e... | 2 | 2 | 3.8% | 4.52E-4 | 70617 |
[ |
YBP2 | Central kinetochore associated protein that mediates mitotic progression; interacts with several cen... | 2 | 3 | 3.7% | 6.71E-4 | 73102 |
[ |
TIF4631 | Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that al... | 2 | 2 | 3.6% | 3.01E-4 | 107101 |
[ |
LHS1 | Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and fo... | 2 | 2 | 3.6% | 3.25E-4 | 99572 |
[ |
RSC30 | Component of the RSC chromatin remodeling complex; non-essential gene required for regulation of rib... | 2 | 2 | 3.5% | 3.45E-4 | 95520 |
[ |
SFB2 | Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat, required for cargo selection du... | 2 | 8 | 3.3% | 0.00131 | 98943 |
[ |
ECM10 | Heat shock protein of the Hsp70 family, localized in mitochondrial nucleoids, plays a role in protei... | 2 | 3 | 3.3% | 6.68E-4 | 70085 |
[ |
SSC1 | Hsp70 family ATPase, constituent of the import motor component of the Translocase of the Inner Mitoc... | 2 | 2 | 3.2% | 4.38E-4 | 70628 |
[ |
KAR2 | ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... | 2 | 3 | 3.1% | 6.31E-4 | 74468 |
[ |
APL2 | Beta-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; invol... | 2 | 4 | 2.9% | 7.9E-4 | 81869 |
[ |
SSM4 | Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear en... | 2 | 4 | 2.6% | 4.35E-4 | 151454 |
[ |
GDE1 | Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphospha... | 2 | 20 | 2.3% | 0.00234 | 138014 |
[ |
GLT1 | NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglut... | 2 | 3 | 2.2% | 2.0E-4 | 238100 |
[ |
YLR001C | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purifi... | 3 | 23 | 2.2% | 0.00383 | 97618 |
[ |
NUP159 | Nucleoporin, subunit of the nuclear pore complex that is found exclusively on the cytoplasmic side, ... | 2 | 2 | 2.1% | 1.96E-4 | 158907 |
[ |
YBR235W | Putative ion transporter, similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family; Y... | 2 | 2 | 2.1% | 2.56E-4 | 123998 |
[ |
MUC1 | GPI-anchored cell surface glycoprotein (flocculin) required for pseudohyphal formation, invasive gro... | 2 | 4 | 1.7% | 4.19E-4 | 136110 |
[ |
TOR2 | PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth i... | 2 | 2 | 1.4% | 1.16E-4 | 281509 |
[ |
URA2 | Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes t... | 2 | 2 | 1.4% | 1.3E-4 | 245123 |
[ |
MDM20 | Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of pro... | 2 | 2 | 1.1% | 3.6E-4 | 92809 |
[ |
TOM1 | E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may reg... | 2 | 4 | 1.0% | 1.75E-4 | 374186 |
[ |
BLM10 | Proteasome activator subunit; found in association with core particles, with and without the 19S reg... | 2 | 3 | 1.0% | 2.01E-4 | 245993 |