Protein Overview: PSE1

General Information:
Name(s) found:	PSE1 / YMR308C [SGD]
Description(s) found: Found 23 descriptions. SHOW ALL	Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p [SGD]
Organism:	Saccharomyces cerevisiae
Length:	1089 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] cytoplasm [IDA]
Biological Process:	mRNA export from nucleus [TAS] protein import into nucleus [IMP]
Molecular Function:	protein transmembrane transporter activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSALPEEVNR TLLQIVQAFA SPDNQIRSVA EKALSEEWIT ENNIEYLLTF LAEQAAFSQD  60
   61 TTVAALSAVL FRKLALKAPP SSKLMIMSKN ITHIRKEVLA QIRSSLLKGF LSERADSIRH 120
  121 KLSDAIAECV QDDLPAWPEL LQALIESLKS GNPNFRESSF RILTTVPYLI TAVDINSILP 180
  181 IFQSGFTDAS DNVKIAAVTA FVGYFKQLPK SEWSKLGILL PSLLNSLPRF LDDGKDDALA 240
  241 SVFESLIELV ELAPKLFKDM FDQIIQFTDM VIKNKDLEPP ARTTALELLT VFSENAPQMC 300
  301 KSNQNYGQTL VMVTLIMMTE VSIDDDDAAE WIESDDTDDE EEVTYDHARQ ALDRVALKLG 360
  361 GEYLAAPLFQ YLQQMITSTE WRERFAAMMA LSSAAEGCAD VLIGEIPKIL DMVIPLINDP 420
  421 HPRVQYGCCN VLGQISTDFS PFIQRTAHDR ILPALISKLT SECTSRVQTH AAAALVNFSE 480
  481 FASKDILEPY LDSLLTNLLV LLQSNKLYVQ EQALTTIAFI AEAAKNKFIK YYDTLMPLLL 540
  541 NVLKVNNKDN SVLKGKCMEC ATLIGFAVGK EKFHEHSQEL ISILVALQNS DIDEDDALRS 600
  601 YLEQSWSRIC RILGDDFVPL LPIVIPPLLI TAKATQDVGL IEEEEAANFQ QYPDWDVVQV 660
  661 QGKHIAIHTS VLDDKVSAME LLQSYATLLR GQFAVYVKEV MEEIALPSLD FYLHDGVRAA 720
  721 GATLIPILLS CLLAATGTQN EELVLLWHKA SSKLIGGLMS EPMPEITQVY HNSLVNGIKV 780
  781 MGDNCLSEDQ LAAFTKGVSA NLTDTYERMQ DRHGDGDEYN ENIDEEEDFT DEDLLDEINK 840
  841 SIAAVLKTTN GHYLKNLENI WPMINTFLLD NEPILVIFAL VVIGDLIQYG GEQTASMKNA 900
  901 FIPKVTECLI SPDARIRQAA SYIIGVCAQY APSTYADVCI PTLDTLVQIV DFPGSKLEEN 960
  961 RSSTENASAA IAKILYAYNS NIPNVDTYTA NWFKTLPTIT DKEAASFNYQ FLSQLIENNS1020
 1021 PIVCAQSNIS AVVDSVIQAL NERSLTEREG QTVISSVKKL LGFLPSSDAM AIFNRYPADI1080
 1081 MEKVHKWFA

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		HAT1 MAM33 PSE1 SLX9 ULP1
View Details	Krogan NJ, et al. (2006)		ALB1 ARX1 DBP9 GLE2 PSE1 RAD27 SLX9 THO1
View Details	Gavin AC, et al. (2006)		PSE1 SLX9 SPA2 ULP1
View Details	Gavin AC, et al. (2002)		ADH1 BDF2 BDH2 BLM10 CDC55 CKB1 DAM1 DBP9 DYN1 ECM1 ECM29 ECM5 EST3 GCN1 GFA1 GLY1 HAT1 HAT2 HHF1, HHF2 HIF1 HSC82 HSM3 ICL1 IML1 INO4 KAP123 KAP95 KRE33 LIP2 LYS12 LYS2 MAC1 MDN1 MDS3 MIS1 MSB1 NIP1 NMD5 NTE1 NUD1 PDA1 PDB1 PFK1 PRE10 PRE2 PRE3 PRE4 PRE5 PRE6 PRE8 PRE9 PRO1 PRT1 PSD2 PSE1 PSH1 PUP3 RGR1 RPA135 RPC40 RPG1 RPN12 RPN3 RPN6 RPN8 RPN9 RPT2 RPT3 RPT5 RPT6 RRP12 RSC3 SAM1 SCL1 SLX9 SPA2 SRP1 SXM1 TEL1 TSC11 ULP1 USO1 UTP20 VTH2 YFL006W YFR006W YMR310C YPR077C YRA1
View Details	Ho Y, et al. (2002)		AAC3 ABP1 AIM46 AIR1 AIR2 ARP4 ASA1 ATG17 ATG29 ATP3 CDC33 CIC1 CIS1 CYS4 FOL2 GRH1 HAP2 HAP5 HHF1, HHF2 HTB1 HTB2 IMD1 IMD3 IMD4 INO4 IPP1 LOC1 MAM33 MTQ1 MTR4 NAP1 NIP7 NMD5 NOP16 NOP53 NOP56 NSA2 PAP2 POL5 PSE1 RHR2 SAH1 SAP190 SPE3 SPO12 SRV2 SSK2 SUM1 TIF1, TIF2 TIF6 TRX2 UTP4 YRA1 YRA2 YTM1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MIF2	No Comments	Cheeseman IM, et al. (2002)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	None Entered	#17 Mitotic Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	PSE1	SOR2	2	Uetz P, et al. (2000)
View Screen	PSE1	ACA1	2	Uetz P, et al. (2000)
View Screen	PSE1	KGD2	2	Uetz P, et al. (2000)
View Screen	PSE1	CSM3	2	Uetz P, et al. (2000)
View Screen	PSE1	MND2	2	Uetz P, et al. (2000)
View Screen	PSE1	NIF3	2	Unpublished Fields Lab Data
View Screen	PSE1	RDS2	2	Uetz P, et al. (2000)
View Screen	PSE1	NUP57	2	Uetz P, et al. (2000)
View Screen	PSE1	SOR1	2	Uetz P, et al. (2000)

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	PSE1	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..246]	PSI-BLAST	83.39794	Importin beta
2	View Details	[247..439]	PSI-BLAST	83.39794	Importin beta
3	View Details	[440..534]	PSI-BLAST	83.39794	Importin beta
4	View Details	[535..632]	PSI-BLAST	83.39794	Importin beta
5	View Details	[633..910]	PSI-BLAST	83.39794	Importin beta
6	View Details	[911..1089]	MSA	2.151995	View MSA. No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
protein transporter activity	4.58709163830613	bayes_pls_golite062009
binding	3.0667965351474	bayes_pls_golite062009
protein transmembrane transporter activity	2.74547353635661	bayes_pls_golite062009
transporter activity	2.48700774379895	bayes_pls_golite062009
substrate-specific transporter activity	2.26725422172281	bayes_pls_golite062009
nuclear export signal receptor activity	2.13470398426489	bayes_pls_golite062009
RNA binding	1.91507786651888	bayes_pls_golite062009
nuclear localization sequence binding	1.91487015967262	bayes_pls_golite062009
protein binding	1.73688475473996	bayes_pls_golite062009
transmembrane transporter activity	1.28481004323799	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	1.09652988954158	bayes_pls_golite062009
nucleic acid binding	1.00841167631924	bayes_pls_golite062009
signal transducer activity	0.662662274021266	bayes_pls_golite062009
molecular transducer activity	0.662662274021266	bayes_pls_golite062009
signal sequence binding	0.57106697498903	bayes_pls_golite062009
transcription regulator activity	0.385452858197632	bayes_pls_golite062009
mRNA binding	0.37278269424461	bayes_pls_golite062009
DNA binding	0.309260217284589	bayes_pls_golite062009
active transmembrane transporter activity	0.252495966234234	bayes_pls_golite062009
ion transmembrane transporter activity	0.248585912061178	bayes_pls_golite062009
primary active transmembrane transporter activity	0.226380819487924	bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity	0.217517113570989	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	0.178770060806735	bayes_pls_golite062009
ATPase activity, coupled to movement of substances	0.175241020572885	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of substances	0.174922130116569	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.100181463251306	bayes_pls_golite062009
pyrophosphatase activity	0.0635404099346928	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.0548082069333885	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.052195223119721	bayes_pls_golite062009
cation transmembrane transporter activity	0.0405268469927988	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.91

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2