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Protein Overview: RAD27

General Information:
Name(s) found: RAD27 / YKL113C [SGD]
Description(s) found:
Found 25 descriptions.
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  • 5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 382 amino acids

    Gene Ontology:
    Cellular Component: nucleus [IMPLink to PubMed article]
    Biological Process: DNA repair [TASLink to PubMed article]
    DNA replication [TASLink to PubMed article]
    telomere maintenance [IMPLink to PubMed article]
    DNA synthesis involved in DNA repair [IMPLink to PubMed article]
    double-strand break repair via nonhomologous end joining [IDALink to PubMed article]
    replicative cell aging [IMPLink to PubMed article]
    Molecular Function: 5'-flap endonuclease activity [IDALink to PubMed article][TASLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MGIKGLNAII SEHVPSAIRK SDIKSFFGRK VAIDASMSLY QFLIAVRQQD GGQLTNEAGE  60
   61 TTSHLMGMFY RTLRMIDNGI KPCYVFDGKP PDLKSHELTK RSSRRVETEK KLAEATTELE 120
  121 KMKQERRLVK VSKEHNEEAQ KLLGLMGIPY IIAPTEAEAQ CAELAKKGKV YAAASEDMDT 180
  181 LCYRTPFLLR HLTFSEAKKE PIHEIDTELV LRGLDLTIEQ FVDLCIMLGC DYCESIRGVG 240
  241 PVTALKLIKT HGSIEKIVEF IESGESNNTK WKIPEDWPYK QARMLFLDPE VIDGNEINLK 300
  301 WSPPKEKELI EYLCDDKKFS EERVKSGISR LKKGLKSGIQ GRLDGFFQVV PKTKEQLAAA 360
  361 AKRAQENKKL NKNKNKVTKG RR

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) ATP16 FIT1 RAD27 RPS26A TIF4632 YMR118C
    View Details Krogan NJ, et al. (2006) ALB1 ARX1 DBP9 GLE2 PSE1 RAD27 SLX9 THO1
    View Details Ho Y, et al. (2002) POL30 RAD27

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #10 Mitotic Prep1-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #11 Mitotic Prep1-TiO2 enriched, new search criteria Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data RAD27 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..90]
    [107..216]
    [287..382]    		 PSI-BLAST 646.703922 Flap endonuclease-1 (Fen-1 nuclease)
    2 View Details [91..106]
    [217..286]    		 PSI-BLAST 646.703922 Flap endonuclease-1 (Fen-1 nuclease)

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • hydrolase activity, acting on ester bonds
    		•  5.44893156272125 bayes_pls_golite062009
  • DNA-directed DNA polymerase activity
    		•  4.17543266498933 bayes_pls_golite062009
  • DNA polymerase activity
    		•  4.1412804577877 bayes_pls_golite062009
  • flap endonuclease activity
    		•  4.10438637013244 bayes_pls_golite062009
  • exonuclease activity
    		•  3.99717729574528 bayes_pls_golite062009
  • single-stranded DNA specific exodeoxyribonuclease activity
    		•  3.90367500308359 bayes_pls_golite062009
  • endonuclease activity
    		•  3.40075034200919 bayes_pls_golite062009
  • endodeoxyribonuclease activity
    		•  3.29138075959109 bayes_pls_golite062009
  • deoxyribonuclease activity
    		•  3.25110450559975 bayes_pls_golite062009
  • 5'-3' exonuclease activity
    		•  3.19536858640519 bayes_pls_golite062009
  • nuclease activity
    		•  2.9643999717473 bayes_pls_golite062009
  • exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters
    		•  2.76402807313057 bayes_pls_golite062009
  • single-stranded DNA specific endodeoxyribonuclease activity
    		•  2.49388502336873 bayes_pls_golite062009
  • hydrolase activity
    		•  2.4418166499042 bayes_pls_golite062009
  • ribonuclease activity
    		•  2.33034300966639 bayes_pls_golite062009
  • 5'-3' exodeoxyribonuclease activity
    		•  2.26658239783943 bayes_pls_golite062009
  • damaged DNA binding
    		•  2.10834170214137 bayes_pls_golite062009
  • double-stranded DNA specific exodeoxyribonuclease activity
    		•  2.09057967200253 bayes_pls_golite062009
  • binding
    		•  2.06833406478856 bayes_pls_golite062009
  • nucleic acid binding
    		•  2.02510018436829 bayes_pls_golite062009
  • exodeoxyribonuclease activity
    		•  2.0005433655378 bayes_pls_golite062009
  • exodeoxyribonuclease activity, producing 5'-phosphomonoesters
    		•  1.9833086883205 bayes_pls_golite062009
  • DNA binding
    		•  1.93844174573408 bayes_pls_golite062009
  • 5'-flap endonuclease activity
    		•  1.81850767854435 bayes_pls_golite062009
  • structure-specific DNA binding
    		•  1.70042864382698 bayes_pls_golite062009
  • catalytic activity
    		•  1.37534898662234 bayes_pls_golite062009
  • ribonuclease H activity
    		•  1.29762010718359 bayes_pls_golite062009
  • single-stranded DNA specific 5'-3' exodeoxyribonuclease activity
    		•  1.15948049342759 bayes_pls_golite062009
  • endodeoxyribonuclease activity, producing 5'-phosphomonoesters
    		•  0.901965019685213 bayes_pls_golite062009
  • double-stranded DNA binding
    		•  0.626785110524684 bayes_pls_golite062009
  • endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters
    		•  0.512509234493511 bayes_pls_golite062009
  • 3'-5' exonuclease activity
    		•  0.50487165366129 bayes_pls_golite062009
  • protein binding
    		•  0.488846604207788 bayes_pls_golite062009
  • nucleotidyltransferase activity
    		•  0.351084053450465 bayes_pls_golite062009
  • transferase activity, transferring phosphorus-containing groups
    		•  0.319622686426271 bayes_pls_golite062009
  • endoribonuclease activity
    		•  0.312346277055223 bayes_pls_golite062009
  • single-stranded DNA specific 3'-5' exodeoxyribonuclease activity
    		•  0.14861832363159 bayes_pls_golite062009
  • 3'-5'-exodeoxyribonuclease activity
    		•  0.0205129272358 bayes_pls_golite062009
    2 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.94

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle