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Protein Overview: RPN3

General Information:
Name(s) found: RPN3 / YER021W [SGD]
Description(s) found:
Found 21 descriptions.
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  • Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 523 amino acids

    Gene Ontology:
    Cellular Component: proteasome regulatory particle, lid subcomplex [IPILink to PubMed article]
    Biological Process: ubiquitin-dependent protein catabolic process [TASLink to PubMed articleLink to PubMed article]
    Molecular Function: molecular_function [ND]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MASTAVMMDV DSSGVNDLHH SEKKYAEEDQ VQELLKVLNE ISKTTLTLDP RYIWRSLKDL  60
   61 SSLRNQELLN AETLCFTVNV LYPDSSSFKK NLLKFITSNH KSSVPGSAEL RNSYPASFYS 120
  121 VNTEKKTIEV TAEINCFMHL LVQLFLWDSK ELEQLVEFNR KVVIPNLLCY YNLRSLNLIN 180
  181 AKLWFYIYLS HETLARSSEE INSDNQNIIL RSTMMKFLKI ASLKHDNETK AMLINLILRD 240
  241 FLNNGEVDSA SDFISKLEYP HTDVSSSLEA RYFFYLSKIN AIQLDYSTAN EYIIAAIRKA 300
  301 PHNSKSLGFL QQSNKLHCCI QLLMGDIPEL SFFHQSNMQK SLLPYYHLTK AVKLGDLKKF 360
  361 TSTITKYKQL LLKDDTYQLC VRLRSNVIKT GIRIISLTYK KISLRDICLK LNLDSEQTVE 420
  421 YMVSRAIRDG VIEAKINHED GFIETTELLN IYDSEDPQQV FDERIKFANQ LHDEYLVSMR 480
  481 YPEDKKTQQN EKSENGENDD DTLDGDLMDD MSDISDLDDL GFL

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) ECM29 NAS6 OSH7 POL4 PRE1 PRE5 RPN1 RPN10 RPN11 RPN12 RPN13 RPN2 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 SEM1 SPG5 UBP6 URA3
    View Details (MIPS) Mewes HW, et al. (2004) NAS6 RPN1 RPN10 RPN11 RPN12 RPN2 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6
    View Details Krogan NJ, et al. (2006) ADE2 BSC2 CWH41 DBF4 DIG1 ECM29 HAL5 LAA1 MRC1 OPI9 PEX32 PRP5 PTH2 RGA2 RMA1 RPN3 SIR1 STE12 THR1 TLG2 VPS45 YDL199C YEL057C YPL035C
    View Details Gavin AC, et al. (2002) ADH1 BDF2 BLM10 CDC55 CDC6 CKB1 DNA2 DYN1 ECM29 ECM5 FET5 FKS1 FTH1 GEA2 GFA1 GLY1 HAT1 HAT2 HHF1, HHF2 HIF1 HSM3 ICL1 IML1 KAP123 KAP95 KRE33 LIP2 MAC1 MDN1 MEC1 MIS1 MLH2 MPH1 MRPS28 MSB1 MSH2 MSH6 NAS6 NIP1 NMD5 PFK1 PGK1 PRE1 PRE10 PRE2 PRE3 PRE4 PRE5 PRE6 PRE7 PRE8 PRE9 PRO1 PRT1 PSD2 PSE1 PSH1 PUP3 RAD23 RAD34 RAD4 RAD52 RFA1 RFA2 RGR1 RPA135 RPC40 RPG1 RPN10 RPN11 RPN12 RPN13 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 RSC3 SAM1 SCL1 SRO7 SSL1 TEL1 TFA1 TFB1 TFB4 TSC11 UBP6 USO1 UTP20 VTH2 YFL006W YFR006W YPR077C
    View Details Ho Y, et al. (2002) AAC1 AAC3 ACC1 ACH1 ADE5,7 ADH2 ADR1 AHA1 ARC1 ARO1 ARP2 ATG11 ATG12 ATG17 CAR2 CPA2 CPR6 FET4 GCD11 GFA1 GND1 GUS1 IDH2 IPP1 ISA2 LHS1 MDH1 MDN1 MET18 MKT1 NAS6 NGL2 PPX1 PRB1 PTC7 PYC1 PYC2 REP1 RET1 REX2 RPA135 RPA190 RPA49 RPB5 RPC19 RPC25 RPC34 RPC40 RPC82 RPN1 RPN10 RPN11 RPN12 RPN13 RPN14 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPO26 RPO31 RPT1 RPT2 RPT3 RPT4 RPT5 RVS167 SEC18 SEC27 STI1 TBS1 TCB1 TFP1 TIF1, TIF2 TSA2 TYR1 UBC12 YFL042C YHR020W YHR033W YHR112C YNL208W
    View Details Qiu et al. (2008) NAS6 PRE4 RPN1 RPN10 RPN11 RPN12 RPN13 RPN2 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run MLP2 #24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #31 Asynchronous Prep (Protease cleavage) Keck JM, et al. (2011)
    View Run MLP2 #10 Mitotic Prep1-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

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      PROTEIN(S) PUBLICATION
    View Data RPN3 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..109] MSA 1.024992 View MSA. Confident ab initio structure predictions are available.
    2 View Details [110..315] ORFEUS 10.38 Transcription factor MalT domain III
    3 View Details [316..447] ORFEUS 10.38 Transcription factor MalT domain III
    4 View Details [448..523] deduced N/A No confident structure predictions are available.

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2
    Term Confidence Notes
  • hydrolase activity
    		•  2.802999698299 bayes_pls_golite062009
  • binding
    		•  2.0556615145105 bayes_pls_golite062009
  • protein binding
    		•  1.85124014295385 bayes_pls_golite062009
  • ubiquitin-protein ligase activity
    		•  0.499034250223658 bayes_pls_golite062009
  • small conjugating protein ligase activity
    		•  0.447205684237625 bayes_pls_golite062009
  • nucleic acid binding
    		•  0.437277381081288 bayes_pls_golite062009
  • transcription regulator activity
    		•  0.198767743012145 bayes_pls_golite062009
  • DNA binding
    		•  0.186035324819401 bayes_pls_golite062009
    3
    Term Confidence Notes
  • transcription regulator activity
    		•  3.38230388954255 bayes_pls_golite062009
  • nucleic acid binding
    		•  3.38144578566524 bayes_pls_golite062009
  • DNA binding
    		•  3.30822066787723 bayes_pls_golite062009
  • binding
    		•  2.93590311675477 bayes_pls_golite062009
  • transcription factor activity
    		•  2.53517246325934 bayes_pls_golite062009
  • hydrolase activity
    		•  2.30467124306022 bayes_pls_golite062009
  • sequence-specific DNA binding
    		•  1.28622713424902 bayes_pls_golite062009
  • protein binding
    		•  1.09170941633583 bayes_pls_golite062009
  • transcription activator activity
    		•  1.08566311616647 bayes_pls_golite062009
  • transcription repressor activity
    		•  0.95491822926452 bayes_pls_golite062009
  • RNA polymerase II transcription factor activity
    		•  0.42135864069093 bayes_pls_golite062009
  • structural molecule activity
    		•  0.272316540117209 bayes_pls_golite062009
  • ubiquitin-protein ligase activity
    		•  0.136726704233672 bayes_pls_golite062009
  • small conjugating protein ligase activity
    		•  0.118016217031359 bayes_pls_golite062009
  • structural constituent of ribosome
    		•  0.0912115683429473 bayes_pls_golite062009
    4 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle