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Protein Overview: TFP1

General Information:
Name(s) found: TFP1 / YDL185W [SGD]
Description(s) found:
Found 28 descriptions.
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  • Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 1071 amino acids

    Gene Ontology:
    Cellular Component: vacuolar proton-transporting V-type ATPase, V1 domain [TASLink to PubMed articleLink to PubMed article]
    fungal-type vacuole membrane [IDALink to PubMed article]
    Biological Process: protein metabolic process [IDALink to PubMed article]
    vacuolar acidification [TASLink to PubMed articleLink to PubMed article]
    intron homing [TASLink to PubMed article]
    Molecular Function: proton-transporting ATPase activity, rotational mechanism [TASLink to PubMed articleLink to PubMed article]
    endodeoxyribonuclease activity [TASLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAGAIENARK EIKRISLEDH AESEYGAIYS VSGPVVIAEN MIGCAMYELV KVGHDNLVGE  60
   61 VIRIDGDKAT IQVYEETAGL TVGDPVLRTG KPLSVELGPG LMETIYDGIQ RPLKAIKEES 120
  121 QSIYIPRGID TPALDRTIKW QFTPGKFQVG DHISGGDIYG SVFENSLISS HKILLPPRSR 180
  181 GTITWIAPAG EYTLDEKILE VEFDGKKSDF TLYHTWPVRV PRPVTEKLSA DYPLLTGQRV 240
  241 LDALFPCVQG GTTCIPGAFG CGKTVISQSL SKYSNSDAII YVGCFAKGTN VLMADGSIEC 300
  301 IENIEVGNKV MGKDGRPREV IKLPRGRETM YSVVQKSQHR AHKSDSSREV PELLKFTCNA 360
  361 THELVVRTPR SVRRLSRTIK GVEYFEVITF EMGQKKAPDG RIVELVKEVS KSYPISEGPE 420
  421 RANELVESYR KASNKAYFEW TIEARDLSLL GSHVRKATYQ TYAPILYEND HFFDYMQKSK 480
  481 FHLTIEGPKV LAYLLGLWIG DGLSDRATFS VDSRDTSLME RVTEYAEKLN LCAEYKDRKE 540
  541 PQVAKTVNLY SKVVRGNGIR NNLNTENPLW DAIVGLGFLK DGVKNIPSFL STDNIGTRET 600
  601 FLAGLIDSDG YVTDEHGIKA TIKTIHTSVR DGLVSLARSL GLVVSVNAEP AKVDMNGTKH 660
  661 KISYAIYMSG GDVLLNVLSK CAGSKKFRPA PAAAFARECR GFYFELQELK EDDYYGITLS 720
  721 DDSDHQFLLA NQVVVHNCGE RGNEMAEVLM EFPELYTEMS GTKEPIMKRT TLVANTSNMP 780
  781 VAAREASIYT GITLAEYFRD QGKNVSMIAD SSSRWAEALR EISGRLGEMP ADQGFPAYLG 840
  841 AKLASFYERA GKAVALGSPD RTGSVSIVAA VSPAGGDFSD PVTTATLGIT QVFWGLDKKL 900
  901 AQRKHFPSIN TSVSYSKYTN VLNKFYDSNY PEFPVLRDRM KEILSNAEEL EQVVQLVGKS 960
  961 ALSDSDKITL DVATLIKEDF LQQNGYSTYD AFCPIWKTFD MMRAFISYHD EAQKAVANGA1020
 1021 NWSKLADSTG DVKHAVSSSK FFEPSRGEKE VHGEFEKLLS TMQERFAEST D

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) TFP1 VMA10 VMA13 VMA4 VMA7 VMA8
    View Details (MIPS) Mewes HW, et al. (2004) CUP5 PPA1 STV1 TFP1 TFP3 VMA10 VMA13 VMA2 VMA4 VMA5 VMA6 VMA7 VMA8 VPH1
    View Details Krogan NJ, et al. (2006) CLG1 CUE1 GTR1 HAM1 HRT1 LAP3 TFP1 VMA10 VMA13 VMA2 VMA7 VMA8 XDJ1 YIG1
    View Details Gavin AC, et al. (2002) AHA1 ASC1 ATG14 ATP11 ATP2 BSP1 BZZ1 CAF130 CAF40 CCR4 CCT6 CDC36 CDC39 CDC45 CHC1 CLU1 COP1 ECM25 ECM29 END3 ERG26 FAS2 GCN1 GCN20 GFA1 GLT1 HAT1 HSP42 INP52 KAP123 LAS17 LSB3 LYS12 MCM2 MCM5 MCM6 MCM7 MET18 MOT2 NOT3 NOT5 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 PDC1 POL1 POL12 POP2 PRI1 PRI2 RAD3 RAD51 RAV1 RPD3 RPN10 RPN9 RPT3 RPT5 RPT6 RVB2 SAM1 SEC27 SIN3 SLA1 SLA2 STM1 SYP1 TFC7 TFP1 TUB3 VMA10 VMA13 VMA2 VMA4 VMA5 VMA6 VMA7 VMA8 VPH1 VPS30 VRP1 YDJ1 YNL313C YPT7 YRA1 YSC84
    View Details Ho Y, et al. (2002) AAC1 AAC3 ACC1 ACH1 ACO1 ADH2 ADH4 ADH6 ADK1 ADR1 ARC1 ARP10 ATG4 ATP3 AVT2 BIO3 CAR2 CCL1 CDC15 CDC33 CKA1 CKA2 CKB1 CKB2 CLU1 COP1 CPR1 CYR1 DBF2 DIP2 DIP5 DUN1 ECM10 ELM1 ERG20 ESL2 FAA1 FET4 FUM1 FYV10 GCD11 GDI1 GFA1 GID7 GID8 GND1 GPH1 GUS1 HAP3 HOR2 HOS4 HSP104 HXT6 HXT7 IDH2 IPP1 ISA2 KIN28 KNS1 KRE33 LCP5 LSC1 MAM33 MDH1 MET18 MGE1 MOB1 MRE11 MUM2 NAN1 NGL2 NOP1 NOP56 NPA3 NTA1 PET112 PGM2 PNO1 POR1 PRB1 PRO1 PRP11 PRP40 PTC5 PTC7 PWP2 PYC1 PYC2 QCR2 RAD1 RAD10 RAD26 RAD3 RAD50 RET1 REX2 RHR2 RMD5 RNR2 RPA135 RPA190 RPA49 RPB5 RPC19 RPC25 RPC34 RPC40 RPC82 RPN1 RPN3 RPN5 RPN8 RPO26 RPO31 RPT3 RPT5 RRP5 RVS167 SAH1 SEC27 SEC53 SHM2 SIF2 SIR3 SNF1 SNT1 SSK2 TBS1 TEF4 TFB3 TFP1 THI22 TIF1, TIF2 TIF4631 TMA29 TPS1 TRM3 TSA2 UBP15 UBP8 UTP10 UTP11 UTP13 UTP14 UTP18 UTP21 UTP22 UTP30 UTP6 VID28 VID30 VMA4 XRS2 YBR184W YEL023C YFL042C YGR016W YGR250C YHR020W YHR112C YMR155W YPK2 YRF1-3, YRF1-7 YSP2 ZDS2
    View Details Qiu et al. (2008) CUP5 PPA1 STV1 TFP1 TFP3 VMA10 VMA13 VMA2 VMA4 VMA5 VMA6 VMA7 VMA8 VMA9 VPH1

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run PGA1 No Comments Hazbun TR, et al. (2003)
    View Run LST8 No Comments Reinke A, et al. (2004)
    View Run RPA135 his-HA tag on RPA135 Schneider, DA, et al. (2006)
    View Run RPA135 his-HA tag on RPA135 Schneider, DA, et al. (2006)
    View Run HIR2 No Comments Green EM, et al (2005)
    View Run RLF2 cac1: TAP-tagged Green EM, et al (2005)
    View Run DAD1 Dam1-765 complex purified from yeast with Dad1-TAP Shimogawa MM, et al. (2006)
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run ZDS1 Sample 2- cpn2 from june 2005 McCusker D, et al (2007)
    View Run BOI1 Sample boi 1 with ha tag from october 2005 McCusker D, et al (2007)
    View Run VAM3 Sample: HX11 - both fusion and trans-SNARE complex formation take place. Hao Xu, et al. (2010)
    View Run VAM3 Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control). Hao Xu, et al. (2010)
    View Run VAM3 Sample: HX13 - trans-SNARE complex forms but fusion is blocked. Hao Xu, et al. (2010)
    View Run VAM3 Sample: HX14 - mixture in detergent (control). Hao Xu, et al. (2010)
    View Run MLP2 #24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #32 Asynchronous Prep-No Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #08 Alpha Factor Prep4-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data TFP1 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..84] Pfam 21.886057 No description for PF02874.7 was found. No confident structure predictions are available.
    2 View Details [85..189] MSA 2.05899 View MSA. No confident structure predictions are available.
    3 View Details [190..261] deduced N/A No confident structure predictions are available.
    4 View Details [262..512] PSI-BLAST 80.0 VMA1-derived endonuclease (VDE) PI-Scei intein; VMA1-derived endonuclease (VDE) PI-SceI
    5 View Details [513..1071] PSI-BLAST 113.0 F1 ATP synthase alpha subunit, domain 3; F1 ATP synthase alpha subunit, domain 1; Central domain of alpha subunit of F1 ATP synthase

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • hydrogen ion transmembrane transporter activity
    		•  8.99485812867371 bayes_pls_golite062009
  • hydrogen ion transporting ATP synthase activity, rotational mechanism
    		•  8.43809246013427 bayes_pls_golite062009
  • monovalent inorganic cation transmembrane transporter activity
    		•  7.28612792538764 bayes_pls_golite062009
  • inorganic cation transmembrane transporter activity
    		•  6.56211055461575 bayes_pls_golite062009
  • proton-transporting ATPase activity, rotational mechanism
    		•  3.88373794136406 bayes_pls_golite062009
  • substrate-specific transmembrane transporter activity
    		•  3.39040720727162 bayes_pls_golite062009
  • ion transmembrane transporter activity
    		•  3.28894636799771 bayes_pls_golite062009
  • hydrogen-exporting ATPase activity, phosphorylative mechanism
    		•  3.20056737251701 bayes_pls_golite062009
  • cation transmembrane transporter activity
    		•  3.03916263027565 bayes_pls_golite062009
  • transporter activity
    		•  2.9325438450934 bayes_pls_golite062009
  • transmembrane transporter activity
    		•  2.77909183361556 bayes_pls_golite062009
  • nucleoside-triphosphatase activity
    		•  2.67498718917813 bayes_pls_golite062009
  • pyrophosphatase activity
    		•  2.60251056808401 bayes_pls_golite062009
  • hydrolase activity, acting on acid anhydrides
    		•  2.58430117129985 bayes_pls_golite062009
  • hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
    		•  2.58115131779782 bayes_pls_golite062009
  • hydrolase activity
    		•  2.48023456865742 bayes_pls_golite062009
  • primary active transmembrane transporter activity
    		•  2.38650577592563 bayes_pls_golite062009
  • P-P-bond-hydrolysis-driven transmembrane transporter activity
    		•  2.38284234201968 bayes_pls_golite062009
  • hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances
    		•  2.34368052096144 bayes_pls_golite062009
  • ATPase activity, coupled to transmembrane movement of substances
    		•  2.33928814826072 bayes_pls_golite062009
  • ATPase activity, coupled to movement of substances
    		•  2.33884554716207 bayes_pls_golite062009
  • active transmembrane transporter activity
    		•  2.28786776436738 bayes_pls_golite062009
  • cation-transporting ATPase activity
    		•  2.2242188318911 bayes_pls_golite062009
  • ATPase activity, coupled
    		•  2.19267030982632 bayes_pls_golite062009
  • binding
    		•  2.15223415484989 bayes_pls_golite062009
  • ATPase activity
    		•  2.14491816112038 bayes_pls_golite062009
  • substrate-specific transporter activity
    		•  1.8085602148752 bayes_pls_golite062009
  • metal ion transmembrane transporter activity
    		•  1.53938373974092 bayes_pls_golite062009
  • nucleic acid binding
    		•  1.50098700711558 bayes_pls_golite062009
  • DNA binding
    		•  1.19194387475416 bayes_pls_golite062009
  • ATPase activity, coupled to transmembrane movement of ions
    		•  1.12292194267086 bayes_pls_golite062009
  • transcription regulator activity
    		•  1.12203014375349 bayes_pls_golite062009
  • catalytic activity
    		•  0.942640875796745 bayes_pls_golite062009
  • DNA-dependent ATPase activity
    		•  0.875943780299382 bayes_pls_golite062009
  • structural constituent of ribosome
    		•  0.869710683000949 bayes_pls_golite062009
  • helicase activity
    		•  0.802129193518991 bayes_pls_golite062009
  • purine nucleotide binding
    		•  0.714145134870091 bayes_pls_golite062009
  • nucleotide binding
    		•  0.708394757192331 bayes_pls_golite062009
  • purine ribonucleotide binding
    		•  0.70300395089804 bayes_pls_golite062009
  • ribonucleotide binding
    		•  0.702990562850678 bayes_pls_golite062009
  • voltage-gated cation channel activity
    		•  0.630907399726593 bayes_pls_golite062009
  • DNA helicase activity
    		•  0.606831357648651 bayes_pls_golite062009
  • voltage-gated channel activity
    		•  0.520434833402482 bayes_pls_golite062009
  • voltage-gated ion channel activity
    		•  0.520010821042307 bayes_pls_golite062009
  • potassium channel activity
    		•  0.516313726033988 bayes_pls_golite062009
  • ATP-dependent helicase activity
    		•  0.329657633591245 bayes_pls_golite062009
  • purine NTP-dependent helicase activity
    		•  0.329657633591245 bayes_pls_golite062009
  • anion transmembrane transporter activity
    		•  0.29111329295693 bayes_pls_golite062009
  • cation channel activity
    		•  0.267140200199812 bayes_pls_golite062009
  • ATP binding
    		•  0.221274151647827 bayes_pls_golite062009
  • protein binding
    		•  0.201869978778282 bayes_pls_golite062009
  • adenyl ribonucleotide binding
    		•  0.198698067988182 bayes_pls_golite062009
  • adenyl nucleotide binding
    		•  0.194178388079667 bayes_pls_golite062009
  • structural molecule activity
    		•  0.191568396055768 bayes_pls_golite062009
  • ion channel activity
    		•  0.17671388431308 bayes_pls_golite062009
  • di-, tri-valent inorganic cation transmembrane transporter activity
    		•  0.168531365787205 bayes_pls_golite062009
  • translation regulator activity
    		•  0.165004576559811 bayes_pls_golite062009
  • channel activity
    		•  0.164130750223518 bayes_pls_golite062009
  • passive transmembrane transporter activity
    		•  0.164130750223518 bayes_pls_golite062009
  • substrate-specific channel activity
    		•  0.154272377180496 bayes_pls_golite062009
  • translation factor activity, nucleic acid binding
    		•  0.144513585153713 bayes_pls_golite062009
  • transcription factor activity
    		•  0.101575043176033 bayes_pls_golite062009
  • four-way junction helicase activity
    		•  0.100226648459719 bayes_pls_golite062009
    2 No functions predicted.
    3 No functions predicted.
    4 No functions predicted.
    5 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle