Protein Overview: TFB3

General Information:
Name(s) found:	TFB3 / YDR460W [SGD]
Description(s) found: Found 34 descriptions. SHOW ALL	Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit [SGD]
Organism:	Saccharomyces cerevisiae
Length:	321 amino acids
Gene Ontology:
Cellular Component:	holo TFIIH complex [IDA][TAS] nucleotide-excision repair factor 3 complex [TAS]
Biological Process:	negative regulation of transcription from RNA polymerase II promoter, mitotic [TAS] nucleotide-excision repair, DNA duplex unwinding [TAS] nucleotide-excision repair [TAS] transcription initiation from RNA polymerase II promoter [TAS ]
Molecular Function:	general RNA polymerase II transcription factor activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLMDEYEENK DMCPICKTDR YLSPDVKFLV NPECYHRICE SCVDRIFSLG PAQCPYKGCD  60
   61 KILRKNKFKT QIFDDVEVEK EVDIRKRVFN VFNKTIDDFN GDLVEYNKYL EEVEDIIYKL 120
  121 DHGIDVAKTE EKLRTYEELN KQLIMNNLER SRTEIESFEQ RQKFEKEMKL KKRLLERQIE 180
  181 EEERMNKEWT KKEIVNRLST TTQDINETIE GVKNTVKLKK SSARRKLEEL NRVLKNNPYF 240
  241 NSNVNVQNSR LKDAVPFTPF NGDREAHPRF TLKGSVYNDP FIKDLEHRKE FIASGFNTNY 300
  301 AYERVLTEAF MGLGCVISEE L

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CCL1 KIN28 RAD3 SSL1 TFB1 TFB3 TFB4
View Details	(MIPS) Mewes HW, et al. (2004)		CCL1 KIN28 RAD2 RAD3 SSL1 SSL2 TFB1 TFB2 TFB3 TFB4
View Details	Krogan NJ, et al. (2006)		CCL1 ESP1 KIN28 RAD3 SSL1 TFB1 TFB3 TFB4
View Details	Gavin AC, et al. (2006)		DCS2 TFB3
View Details	Gavin AC, et al. (2002)		CCL1 DCS2 FAP1 FPR1 KAP95 KIN28 MRPS28 SPT7 SSL1 TFA1 TFB1 TFB3 TFB4 TIF6
View Details	Ho Y, et al. (2002)		AAC1 AAC3 ATP3 IDH2 KIN28 MDH1 MET18 RAD3 RPN8 TFB3 TFP1 YBR184W
View Details	Qiu et al. (2008)		ABF1 CCL1 ELC1 KIN28 RAD14 RAD2 RAD3 SSL1 SSL2 TFA2 TFB1 TFB2 TFB3 TFB4 TFB5

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	MEC3	TFB3	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	TFB3	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..67]	PSI-BLAST	127.035458	TFIIH Mat1 subunit
2	View Details	[68..264]	PSI-BLAST	5.0	Heavy meromyosin subfragment
3	View Details	[265..321]	deduced	N/A	Confident ab initio structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
general RNA polymerase II transcription factor activity	6.51043144330453	bayes_pls_golite062009
ubiquitin-protein ligase activity	5.97121728620188	bayes_pls_golite062009
small conjugating protein ligase activity	5.78935175140238	bayes_pls_golite062009
acid-amino acid ligase activity	4.75377804653841	bayes_pls_golite062009
ligase activity, forming carbon-nitrogen bonds	3.82459130917693	bayes_pls_golite062009
binding	2.78710909602478	bayes_pls_golite062009
transcription regulator activity	2.72498741013971	bayes_pls_golite062009
nucleic acid binding	2.32387297115017	bayes_pls_golite062009
DNA binding	2.00904221080834	bayes_pls_golite062009
transcription repressor activity	1.98209395225978	bayes_pls_golite062009
protein binding	1.5975811408548	bayes_pls_golite062009
transcription factor activity	1.22579085356684	bayes_pls_golite062009
small conjugating protein-specific protease activity	1.2126425713931	bayes_pls_golite062009
transcription factor binding	1.11842175076436	bayes_pls_golite062009
ubiquitin-specific protease activity	1.03469202541773	bayes_pls_golite062009
RNA polymerase II transcription factor activity	0.90321253993065	bayes_pls_golite062009
ligase activity	0.63517530468004	bayes_pls_golite062009
cysteine-type peptidase activity	0.50197530717959	bayes_pls_golite062009
transcription corepressor activity	0.384990496120483	bayes_pls_golite062009
ubiquitin thiolesterase activity	0.265769062717221	bayes_pls_golite062009
transcription coactivator activity	0.255669498391574	bayes_pls_golite062009
transcription cofactor activity	0.233838999434784	bayes_pls_golite062009
catalytic activity	0.14112008370434	bayes_pls_golite062009
transcription activator activity	0.126093965701234	bayes_pls_golite062009
enzyme binding	0.104373352314595	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2