Protein Overview: TFA1

General Information:
Name(s) found:	TFA1 / YKL028W [SGD]
Description(s) found: Found 23 descriptions. SHOW ALL	TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening [SGD]
Organism:	Saccharomyces cerevisiae
Length:	482 amino acids
Gene Ontology:
Cellular Component:	mitochondrion [IDA] transcription factor TFIIE complex [TAS]
Biological Process:	transcription initiation from RNA polymerase II promoter [TAS ]
Molecular Function:	general RNA polymerase II transcription factor activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDRPIDDIVK NLLKFVVRGF YGGSFVLVLD AILFHSVLAE DDLKQLLSIN KTELGPLIAR  60
   61 LRSDRLISIH KQREYPPNSK SVERVYYYVK YPHAIDAIKW KVHQVVQRLK DDLDKNSEPN 120
  121 GYMCPICLTK YTQLEAVQLL NFDRTEFLCS LCDEPLVEDD SGKKNKEKQD KLNRLMDQIQ 180
  181 PIIDSLKKID DSRIEENTFE IALARLIPPQ NQSHAAYTYN PKKGSTMFRP GDSAPLPNLM 240
  241 GTALGNDSSR RAGANSQATL HINITTASDE VAQRELQERQ AEEKRKQNAV PEWHKQSTIG 300
  301 KTALGRLDNE EEFDPVVTAS AMDSINPDNE PAQETSYQNN RTLTEQEMEE RENEKTLNDY 360
  361 YAALAKKQAK LNKEEEEEEE EEEDEEEEEE EEMEDVMDDN DETARENALE DEFEDVTDTA 420
  421 GTAKTESNTS NDVKQESIND KTEDAVNATA TASGPSANAK PNDGDDDDDD DDDEMDIEFE 480
  481 DV

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		TFA1 TFA2
View Details	(MIPS) Mewes HW, et al. (2004)		TFA1 TFA2
View Details	Krogan NJ, et al. (2006)		CWP2 DBP2 FLC2 PPN1 RPS31 SRP40 TFA1 TFA2 YAP1801
View Details	Gavin AC, et al. (2002)		ASC1 CCL1 DCS2 DNA2 ECM29 FAP1 FPR1 KAP95 KIN28 MEC1 MPH1 MRPS28 MSH2 MSH6 PGK1 RAD23 RAD34 RAD4 RAD52 RFA1 RFA2 RPN10 RPN12 RPN3 RPN6 RPN8 RPN9 RPT2 RPT3 RPT6 SPT7 SRO7 SSL1 TFA1 TFB1 TFB3 TFB4 TIF6 TMA20 TMA22
View Details	Qiu et al. (2008)		MED11 TAF10 TAF11 TAF12 TAF14 TAF2 TAF6 TAF7 TFA1 TFA2 TFB1 TFB2 TFB4 TFB5 TFC4 TFC8 TFG2 TOA1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	TFA1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..219]	Pfam	96.420216	TFIIE alpha subunit No confident structure predictions are available.
2	View Details	[220..482]	MSA	7.070997	View MSA. No confident structure predictions are available.
3	View Details	[167..482]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

#

Gene Ontology predictions

1

Term	Confidence	Notes
general RNA polymerase II transcription factor activity	5.67074370996647	bayes_pls_golite062009
transcription regulator activity	5.55329976718261	bayes_pls_golite062009
DNA binding	4.84466441605416	bayes_pls_golite062009
nucleic acid binding	4.57954522145536	bayes_pls_golite062009
transcription factor activity	4.12685284895733	bayes_pls_golite062009
binding	4.05190810999633	bayes_pls_golite062009
RNA polymerase II transcription factor activity	2.60738445921725	bayes_pls_golite062009
sequence-specific DNA binding	2.3918869755162	bayes_pls_golite062009
transcription activator activity	2.37668609508629	bayes_pls_golite062009
transcription repressor activity	2.22485867684613	bayes_pls_golite062009
DNA-directed RNA polymerase activity	1.82365519759474	bayes_pls_golite062009
RNA polymerase activity	1.82365519759474	bayes_pls_golite062009
protein binding	0.961087148890615	bayes_pls_golite062009
transcription factor binding	0.85714789329232	bayes_pls_golite062009
specific RNA polymerase II transcription factor activity	0.66743181561551	bayes_pls_golite062009
nucleotidyltransferase activity	0.237727660028553	bayes_pls_golite062009
RNA polymerase II transcription factor activity, enhancer binding	0.0360186926687502	bayes_pls_golite062009

2

Term	Confidence	Notes
general RNA polymerase II transcription factor activity	7.54088990789822	bayes_pls_golite062009
DNA-directed RNA polymerase activity	6.31798033897642	bayes_pls_golite062009
RNA polymerase activity	6.31798033897642	bayes_pls_golite062009
nucleotidyltransferase activity	3.73777657415367	bayes_pls_golite062009
transcription regulator activity	3.36253105801209	bayes_pls_golite062009
binding	2.58311326284197	bayes_pls_golite062009
transcription elongation regulator activity	2.03339710864117	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	1.95080397433956	bayes_pls_golite062009
nucleic acid binding	1.8086747972572	bayes_pls_golite062009
DNA binding	1.76634962183736	bayes_pls_golite062009
RNA polymerase II transcription factor activity	1.57988053581899	bayes_pls_golite062009
DNA primase activity	1.01743034310838	bayes_pls_golite062009
protein binding	0.764662055834369	bayes_pls_golite062009
catalytic activity	0.434839928397784	bayes_pls_golite062009

3

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

Philius confidence legend

YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle