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Protein Overview: RFA2

General Information:
Name(s) found: RFA2 / YNL312W [SGD]
Description(s) found:
Found 26 descriptions.
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  • Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 273 amino acids

    Gene Ontology:
    Cellular Component: chromosome, telomeric region [IMPLink to PubMed article]
    DNA replication factor A complex [TASLink to PubMed article]
    Biological Process: DNA strand elongation involved in DNA replication [TASLink to PubMed article]
    double-strand break repair via homologous recombination [IGILink to PubMed article]
    DNA recombination [TASLink to PubMed article]
    nucleotide-excision repair [TASLink to PubMed article]
    DNA unwinding involved in replication [TASLink to PubMed article]
    postreplication repair [TASLink to PubMed article]
    DNA replication, synthesis of RNA primer [TASLink to PubMed article]
    Molecular Function: DNA binding [TASLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MATYQPYNEY SSVTGGGFEN SESRPGSGES ETNTRVNTLT PVTIKQILES KQDIQDGPFV  60
   61 SHNQELHHVC FVGVVRNITD HTANIFLTIE DGTGQIEVRK WSEDANDLAA GNDDSSGKGY 120
  121 GSQVAQQFEI GGYVKVFGAL KEFGGKKNIQ YAVIKPIDSF NEVLTHHLEV IKCHSIASGM 180
  181 MKQPLESASN NNGQSLFVKD DNDTSSGSSP LQRILEFCKK QCEGKDANSF AVPIPLISQS 240
  241 LNLDETTVRN CCTTLTDQGF IYPTFDDNNF FAL

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) DNA2 MPH1 RAD52 RFA1 RFA2 RFA3 RIM1 RTT105
    View Details (MIPS) Mewes HW, et al. (2004) RFA1 RFA2 RFA3
    View Details Krogan NJ, et al. (2006) DNA2 HSP12 PRO1 RFA1 RFA2 RFA3
    View Details Gavin AC, et al. (2002) CMR1 DNA2 ECM29 HDA1 HDA2 HDA3 HSC82 KAP95 LCD1 MEC1 MGM101 MLH1 MPH1 MRPS28 MSH2 MSH3 MSH6 OLA1 PGK1 PMS1 PRO2 RAD16 RAD23 RAD34 RAD4 RAD52 RFA1 RFA2 RFA3 RIM1 RNH1 RPN10 RPN12 RPN3 RPN6 RPN8 RPN9 RPT2 RPT3 RPT6 RSC8 RVB2 SGS1 SHS1 SRO7 SSL1 TFA1 TFB1 TFB4 TIF1, TIF2 TOP3 VAS1 VPS1 YHR122W
    View Details Ho Y, et al. (2002) AHP1 CDC10 GCD11 HIR3 HTB1 MGM101 RFA2
    View Details Qiu et al. (2008) MCM4 MCM6 PRI2 RFA1 RFA2 RFA3 RFC3 RFC4

    Mass Spectrometry Data

    No mass spectrometry results found for this protein.

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

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      PROTEIN(S) PUBLICATION
    View Data RFA2 and NOP56 Huh WK, et al. (2003)
    View Data RFA2 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..178] PSI-BLAST 142.69897 Replication protein A 32 KDa subunit (RPA32) fragment
    2 View Details [179..273] PSI-BLAST 25.69897 C-terminal domain of RPA32

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • telomeric DNA binding
    		•  7.07761964836826 bayes_pls_golite062009
  • single-stranded telomeric DNA binding
    		•  6.04637014849887 bayes_pls_golite062009
  • telomerase inhibitor activity
    		•  5.40477712257453 bayes_pls_golite062009
  • nucleic acid binding
    		•  4.02967008021862 bayes_pls_golite062009
  • DNA binding
    		•  3.62735703519187 bayes_pls_golite062009
  • single-stranded DNA binding
    		•  3.59212997362902 bayes_pls_golite062009
  • structure-specific DNA binding
    		•  3.53214384646348 bayes_pls_golite062009
  • binding
    		•  2.63617204791771 bayes_pls_golite062009
  • structural constituent of ribosome
    		•  2.25006883439646 bayes_pls_golite062009
  • transcription regulator activity
    		•  2.20239961025491 bayes_pls_golite062009
  • structural molecule activity
    		•  2.07809455998137 bayes_pls_golite062009
  • DNA replication origin binding
    		•  1.84819558112187 bayes_pls_golite062009
  • ribonuclease activity
    		•  1.19236946227657 bayes_pls_golite062009
  • DNA helicase activity
    		•  1.12203697857261 bayes_pls_golite062009
  • transcription factor activity
    		•  1.05816813143865 bayes_pls_golite062009
  • sequence-specific DNA binding
    		•  0.86170694706755 bayes_pls_golite062009
  • DNA-directed RNA polymerase activity
    		•  0.79554655100991 bayes_pls_golite062009
  • RNA polymerase activity
    		•  0.79554655100991 bayes_pls_golite062009
  • hydrolase activity
    		•  0.724860465280978 bayes_pls_golite062009
  • DNA-dependent ATPase activity
    		•  0.638831894247026 bayes_pls_golite062009
  • nuclease activity
    		•  0.57381531077345 bayes_pls_golite062009
  • DEAD/H-box RNA helicase binding
    		•  0.440977654731901 bayes_pls_golite062009
  • translation regulator activity
    		•  0.41164963411369 bayes_pls_golite062009
  • double-stranded DNA binding
    		•  0.378383789953688 bayes_pls_golite062009
  • translation factor activity, nucleic acid binding
    		•  0.360862776629591 bayes_pls_golite062009
  • nucleotidyltransferase activity
    		•  0.325774164899685 bayes_pls_golite062009
  • four-way junction helicase activity
    		•  0.31769813397725 bayes_pls_golite062009
  • RNA binding
    		•  0.31028563542112 bayes_pls_golite062009
  • translation initiation factor activity
    		•  0.30211828233765 bayes_pls_golite062009
  • exonuclease activity
    		•  0.28313884527829 bayes_pls_golite062009
  • transferase activity, transferring phosphorus-containing groups
    		•  0.226916777208049 bayes_pls_golite062009
  • hydrolase activity, acting on ester bonds
    		•  0.223941674251642 bayes_pls_golite062009
  • exoribonuclease activity, producing 5'-phosphomonoesters
    		•  0.125256208482441 bayes_pls_golite062009
  • exoribonuclease activity
    		•  0.109774918833391 bayes_pls_golite062009
  • exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters
    		•  0.0954216620147701 bayes_pls_golite062009
  • protein binding
    		•  0.0528188351789111 bayes_pls_golite062009
  • ATP-dependent DNA helicase activity
    		•  0.0179535526984695 bayes_pls_golite062009
  • helicase activity
    		•  0.0121541775159524 bayes_pls_golite062009
    2 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle