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Protein Overview: RNH1

General Information:
Name(s) found: RNH1 / YMR234W [SGD]
Description(s) found:
Found 23 descriptions.
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  • Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201) [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 348 amino acids

    Gene Ontology:
    Cellular Component: nucleus [ICLink to PubMed article]
    intracellular [TASLink to PubMed article]
    Biological Process: cellular cell wall organization [IMPLink to PubMed article]
    DNA replication, removal of RNA primer [TASLink to PubMed article]
    Molecular Function: ribonuclease H activity [IDALink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MARQGNFYAV RKGRETGIYN TWNECKNQVD GYGGAIYKKF NSYEQAKSFL GQPNTTSNYG  60
   61 SSTHAGGQVS KPHTTQKRVH RRNRPLHYSS LTSSSACSSL SSANTNTFYS VKSNVPNIES 120
  121 KIFNNWKDCQ AYVKHKRGIT FKKFEDQLAA ENFISGMSAH DYKLMNISKE SFESKYKLSS 180
  181 NTMYNKSMNV YCDGSSFGNG TSSSRAGYGA YFEGAPEENI SEPLLSGAQT NNRAEIEAVS 240
  241 EALKKIWEKL TNEKEKVNYQ IKTDSEYVTK LLNDRYMTYD NKKLEGLPNS DLIVPLVQRF 300
  301 VKVKKYYELN KECFKNNGKF QIEWVKGHDG DPGNEMADFL AKKGASRR

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Gavin AC, et al. (2002) CMR1 DNA2 HDA1 HDA2 HDA3 HSC82 KAP95 LCD1 MEC1 MGM101 MLH1 MPH1 MSH2 MSH3 MSH6 OLA1 PGK1 PMS1 PRO2 RAD16 RAD52 RFA1 RFA2 RFA3 RIM1 RNH1 RSC8 RVB2 SGS1 SHS1 TIF1, TIF2 TOP3 VAS1 VPS1 YHR122W

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run MLP2 #13 Mitotic Prep2-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data RNH1 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..47] PSI-BLAST 205.190949 N-terminal domain of RNase HI
    2 View Details [48..175] PSI-BLAST 87.69897 HIV RNase H (Domain of reverse transcriptase); HIV-1 reverse transcriptase
    3 View Details [176..348] PSI-BLAST 87.69897 HIV RNase H (Domain of reverse transcriptase); HIV-1 reverse transcriptase

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • ribonuclease activity
    		•  4.81599779544001 bayes_pls_golite062009
  • exoribonuclease activity
    		•  3.01417328311605 bayes_pls_golite062009
  • ribonuclease H activity
    		•  2.91966688044755 bayes_pls_golite062009
  • exoribonuclease activity, producing 5'-phosphomonoesters
    		•  2.89077854960958 bayes_pls_golite062009
  • nuclease activity
    		•  2.65398446409354 bayes_pls_golite062009
  • endonuclease activity
    		•  2.63074055051349 bayes_pls_golite062009
  • endoribonuclease activity
    		•  2.59247838057313 bayes_pls_golite062009
  • hydrolase activity, acting on ester bonds
    		•  2.57498413015114 bayes_pls_golite062009
  • 3'-5'-exoribonuclease activity
    		•  2.49957333962564 bayes_pls_golite062009
  • hydrolase activity
    		•  2.41899414621161 bayes_pls_golite062009
  • exonuclease activity
    		•  1.4327316829508 bayes_pls_golite062009
  • catalytic activity
    		•  1.25233849524628 bayes_pls_golite062009
  • nucleic acid binding
    		•  1.23371034593624 bayes_pls_golite062009
  • binding
    		•  1.20709976964365 bayes_pls_golite062009
  • transcription regulator activity
    		•  1.10433412699603 bayes_pls_golite062009
  • 3'-5' exonuclease activity
    		•  0.86862629020506 bayes_pls_golite062009
  • DNA binding
    		•  0.860359347662869 bayes_pls_golite062009
  • endoribonuclease activity, producing 5'-phosphomonoesters
    		•  0.708753843279937 bayes_pls_golite062009
  • endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters
    		•  0.598360955770972 bayes_pls_golite062009
  • exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters
    		•  0.389326716045122 bayes_pls_golite062009
  • transcription factor activity
    		•  0.332903382446869 bayes_pls_golite062009
  • protein binding
    		•  0.242714557118293 bayes_pls_golite062009
    2 No functions predicted.
    3 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.97

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle