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Protein Overview: TOP3

General Information:
Name(s) found: TOP3 / YLR234W [SGD]
Description(s) found:
Found 24 descriptions.
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  • DNA Topoisomerase III, conserved protein that functions in a complex with Sgs1p and Rmi1p to relax single-stranded negatively-supercoiled DNA preferentially, involved in telomere stability and regulation of mitotic recombination [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 656 amino acids

    Gene Ontology:
    Cellular Component: nucleus [ICLink to PubMed article]
    RecQ helicase-Topo III complex [IDALink to PubMed article][IPILink to PubMed article]
    Biological Process: reciprocal meiotic recombination [IGILink to PubMed article][IMPLink to PubMed article]
    telomere maintenance via telomerase [IMPLink to PubMed article]
    regulation of DNA recombination [IMPLink to PubMed article]
    Molecular Function: DNA topoisomerase type I activity [IDALink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKVLCVAEKN SIAKAVSQIL GGGRSTSRDS GYMYVKNYDF MFSGFPFARN GANCEVTMTS  60
   61 VAGHLTGIDF SHDSHGWGKC AIQELFDAPL NEIMNNNQKK IASNIKREAR NADYLMIWTD 120
  121 CDREGEYIGW EIWQEAKRGN RLIQNDQVYR AVFSHLERQH ILNAARNPSR LDMKSVHAVG 180
  181 TRIEIDLRAG VTFTRLLTET LRNKLRNQAT MTKDGAKHRG GNKNDSQVVS YGTCQFPTLG 240
  241 FVVDRFERIR NFVPEEFWYI QLVVENKDNG GTTTFQWDRG HLFDRLSVLT FYETCIETAG 300
  301 NVAQVVDLKS KPTTKYRPLP LTTVELQKNC ARYLRLNAKQ SLDAAEKLYQ KGFISYPRTE 360
  361 TDTFPHAMDL KSLVEKQAQL DQLAAGGRTA WASYAASLLQ PENTSNNNKF KFPRSGSHDD 420
  421 KAHPPIHPIV SLGPEANVSP VERRVYEYVA RHFLACCSED AKGQSMTLVL DWAVERFSAS 480
  481 GLVVLERNFL DVYPWARWET TKQLPRLEMN ALVDIAKAEM KAGTTAPPKP MTESELILLM 540
  541 DTNGIGTDAT IAEHIDKIQV RNYVRSEKVG KETYLQPTTL GVSLVHGFEA IGLEDSFAKP 600
  601 FQRREMEQDL KKICEGHASK TDVVKDIVEK YRKYWHKTNA CKNTLLQVYD RVKASM

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Gavin AC, et al. (2002) CMR1 DNA2 HDA1 HDA2 HDA3 HSC82 KAP95 LCD1 MEC1 MGM101 MLH1 MPH1 MSH2 MSH3 MSH6 OLA1 PGK1 PMS1 PRO2 RAD16 RAD52 RFA1 RFA2 RFA3 RIM1 RNH1 RSC8 RVB2 SGS1 SHS1 TIF1, TIF2 TOP3 VAS1 VPS1 YHR122W
    View Details Qiu et al. (2008) SGS1 TOP3

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run MLP2 #31 Asynchronous Prep (Protease cleavage) Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

      BAIT PREY HITS PUBLICATION
    View Screen TOP3 DUN1 2 Unpublished Fields Lab Data
    View Screen SGS1 TOP3 2 Unpublished Fields Lab Data

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data TOP3 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..37]
    [93..174]    		 PSI-BLAST 408.9897 DNA topoisomerase I, 67K N-terminal domain
    2 View Details [38..92]
    [175..253]
    [526..656]    		 PSI-BLAST 408.9897 DNA topoisomerase I, 67K N-terminal domain
    3 View Details [254..312]
    [464..483]
    [500..525]    		 PSI-BLAST 408.9897 DNA topoisomerase I, 67K N-terminal domain
    4 View Details [313..463]
    [484..499]    		 PSI-BLAST 408.9897 DNA topoisomerase I, 67K N-terminal domain

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • DNA topoisomerase activity
    		•  10.2798831210729 bayes_pls_golite062009
  • DNA topoisomerase type I activity
    		•  8.04585827786358 bayes_pls_golite062009
  • isomerase activity
    		•  5.61256457337998 bayes_pls_golite062009
  • DNA binding
    		•  5.20730175008144 bayes_pls_golite062009
  • nucleic acid binding
    		•  4.95671919332271 bayes_pls_golite062009
  • binding
    		•  4.3089127577748 bayes_pls_golite062009
  • catalytic activity
    		•  1.50449367975676 bayes_pls_golite062009
  • protein binding
    		•  0.377429709958808 bayes_pls_golite062009
    2 No functions predicted.
    3 No functions predicted.
    4 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle