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View Structure Prediction Details

Protein: RFA2
Organism: Saccharomyces cerevisiae
Length: 273 amino acids
Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076



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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for RFA2.

Description E-value Query
Range		 Subject
Range
RFA2 - Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded D...
gi|151944237 - gi|151944237|gb|EDN62516.1| replication factor RF-A subunit 2 [Saccharomyces cerevisiae YJM789]
gi|190409276 - gi|190409276|gb|EDV12541.1| Replication factor-A protein 2 [Saccharomyces cerevisiae RM11-1a]
RFA2_YEAST - Replication factor A protein 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RFA2 PE=1...
 3.0E-85 [1..273] [1..273]
RFA2_RAT - Replication protein A 32 kDa subunit OS=Rattus norvegicus GN=Rpa2 PE=2 SV=2
 1.0E-82 [2..272] [5..262]
gi|13435424, gi|... - gi|31981513|ref|NP_035414.2| replication protein A2 [Mus musculus], gi|13435424|gb|AAH04578.1| Repli...
 1.0E-81 [2..272] [9..266]
RPA2 - replication protein A2, 32kDa
gi|123998291, gi... - gi|123998291|gb|ABM86747.1| replication protein A2, 32kDa [synthetic construct], gi|123983074|gb|ABM...
 2.0E-78 [2..272] [9..266]
RFA2A_ARATH - Replication protein A 32 kDa subunit A OS=Arabidopsis thaliana GN=RPA2A PE=1 SV=2
 2.0E-72 [1..273] [1..277]
RFA2_SCHPO - Replication factor A protein 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=ssb2 PE=1 S...
ssb2 - single-stranded DNA binding protein Ssb2
 6.0E-72 [5..273] [17..275]
RFA2A_ORYSJ - Replication protein A 32 kDa subunit A OS=Oryza sativa subsp. japonica GN=RPA2A PE=1 SV=1
gi|9801268 - gi|9801268|emb|CAC03572.1| replication protein A2 [Oryza sativa]
 4.0E-63 [6..272] [3..275]

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Predicted Domain #1
Region A:
Residues: [1-178] 
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MATYQPYNEY SSVTGGGFEN SESRPGSGES ETNTRVNTLT PVTIKQILES KQDIQDGPFV  60
   61 SHNQELHHVC FVGVVRNITD HTANIFLTIE DGTGQIEVRK WSEDANDLAA GNDDSSGKGY 120
  121 GSQVAQQFEI GGYVKVFGAL KEFGGKKNIQ YAVIKPIDSF NEVLTHHLEV IKCHSIAS

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 142.69897
Match: 1quqA_
Description: Replication protein A 32 KDa subunit (RPA32) fragment
Matching Structure (courtesy of the PDB):

Predicted functions:

Term Confidence Notes
telomeric DNA binding 7.07761964836826 bayes_pls_golite062009
single-stranded telomeric DNA binding 6.04637014849887 bayes_pls_golite062009
telomerase inhibitor activity 5.40477712257453 bayes_pls_golite062009
nucleic acid binding 4.02967008021862 bayes_pls_golite062009
DNA binding 3.62735703519187 bayes_pls_golite062009
single-stranded DNA binding 3.59212997362902 bayes_pls_golite062009
structure-specific DNA binding 3.53214384646348 bayes_pls_golite062009
binding 2.63617204791771 bayes_pls_golite062009
structural constituent of ribosome 2.25006883439646 bayes_pls_golite062009
transcription regulator activity 2.20239961025491 bayes_pls_golite062009
structural molecule activity 2.07809455998137 bayes_pls_golite062009
DNA replication origin binding 1.84819558112187 bayes_pls_golite062009
ribonuclease activity 1.19236946227657 bayes_pls_golite062009
DNA helicase activity 1.12203697857261 bayes_pls_golite062009
transcription factor activity 1.05816813143865 bayes_pls_golite062009
sequence-specific DNA binding 0.86170694706755 bayes_pls_golite062009
DNA-directed RNA polymerase activity 0.79554655100991 bayes_pls_golite062009
RNA polymerase activity 0.79554655100991 bayes_pls_golite062009
hydrolase activity 0.724860465280978 bayes_pls_golite062009
DNA-dependent ATPase activity 0.638831894247026 bayes_pls_golite062009
nuclease activity 0.57381531077345 bayes_pls_golite062009
DEAD/H-box RNA helicase binding 0.440977654731901 bayes_pls_golite062009
translation regulator activity 0.41164963411369 bayes_pls_golite062009
double-stranded DNA binding 0.378383789953688 bayes_pls_golite062009
translation factor activity, nucleic acid binding 0.360862776629591 bayes_pls_golite062009
nucleotidyltransferase activity 0.325774164899685 bayes_pls_golite062009
four-way junction helicase activity 0.31769813397725 bayes_pls_golite062009
RNA binding 0.31028563542112 bayes_pls_golite062009
translation initiation factor activity 0.30211828233765 bayes_pls_golite062009
exonuclease activity 0.28313884527829 bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups 0.226916777208049 bayes_pls_golite062009
hydrolase activity, acting on ester bonds 0.223941674251642 bayes_pls_golite062009
exoribonuclease activity, producing 5'-phosphomonoesters 0.125256208482441 bayes_pls_golite062009
exoribonuclease activity 0.109774918833391 bayes_pls_golite062009
exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters 0.0954216620147701 bayes_pls_golite062009
protein binding 0.0528188351789111 bayes_pls_golite062009
ATP-dependent DNA helicase activity 0.0179535526984695 bayes_pls_golite062009
helicase activity 0.0121541775159524 bayes_pls_golite062009

Predicted Domain #2
Region A:
Residues: [179-273] 
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 GMMKQPLESA SNNNGQSLFV KDDNDTSSGS SPLQRILEFC KKQCEGKDAN SFAVPIPLIS  60
   61 QSLNLDETTV RNCCTTLTDQ GFIYPTFDDN NFFAL

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 25.69897
Match: 1dpuA_
Description: C-terminal domain of RPA32
Matching Structure (courtesy of the PDB):
YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle