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Bait Protein: VAM3
Organism: Saccharomyces cerevisiae
Publication: Hao Xu , Youngsoo Jun, James Thompson, John Yates and William Wickner (2010) HOPS Prevents the Disassembly of Trans-SNARE Complexes by Sec17p/Sec18p during Membrane Fusion [In Press]
Comments: Sample: HX14 - mixture in detergent (control).

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   SEC17 Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' ste... 20 66 61.6% 0.0418 32803
[View Peptides]   RPP2B Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction ... 4 6 49.1% 0.0101 11050
[View Peptides]   VAM7 Component of the vacuole SNARE complex involved in vacuolar morphogenesis; SNAP-25 homolog; function... 14 53 48.7% 0.031 36711
[View Peptides]   VAM3 Syntaxin-related protein required for vacuolar assembly; functions with Vam7p in vacuolar protein tr... 16 148 44.2% 0.0967 32498
[View Peptides]   YKT6 Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and wit... 7 11 40.0% 0.0102 22707
[View Peptides]   PDA1 E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarbo... 17 35 39.8% 0.0154 46343
[View Peptides]   RPP2A Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction... 3 6 36.8% 0.0105 10746
[View Peptides]   VTI1 Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and ... 6 43 35.9% 0.0366 24668
[View Peptides]   SEC18 ATPase required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuol... 19 39 34.3% 0.00951 84056
[View Peptides]   CDC19 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... 10 18 29.6% 0.00665 54545
[View Peptides]   IVY1 Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the... 9 13 26.3% 0.0053 49976
[View Peptides]   KAR2 ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... 12 34 25.4% 0.00922 74468
[View Peptides]   PDB1 E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved mu... 6 33 24.9% 0.0167 40054
[View Peptides]   YNL058C Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to th... 4 8 24.4% 0.00468 35046
[View Peptides]   SNC1 Vesicle membrane receptor protein (v-SNARE) involved in the fusion between Golgi-derived secretory v... 2 4 23.9% 0.00632 13201
[View Peptides]   YPT7 Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vac... 3 3 23.6% 0.00267 23043
[View Peptides]   YPT31 Rab family GTPase, very similar to Ypt32p; involved in the exocytic pathway; mediates intra-Golgi tr... 3 3 22.0% 0.00249 24469
[View Peptides]   DAP2 Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membra... 12 25 21.1% 0.00565 93404
[View Peptides]   LAT1 Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes... 8 21 21.0% 0.00805 51818
[View Peptides]   SNC2 Vesicle membrane receptor protein (v-SNARE) involved in the fusion between Golgi-derived secretory v... 2 2 20.0% 0.00321 12957
[View Peptides]   TSA1 Thioredoxin peroxidase, acts as both a ribosome-associated and free cytoplasmic antioxidant; self-as... 3 3 19.9% 0.00283 21590
[View Peptides]   VPH1 Subunit a of vacuolar-ATPase V0 domain, one of two isoforms (Vph1p and Stv1p); Vph1p is located in V... 12 27 19.9% 0.00594 95529
[View Peptides]   GOS1 v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 3 3 19.7% 0.00249 25395
[View Peptides]   PPN1 Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer 9 25 18.2% 0.00686 78344
[View Peptides]   PSA1 GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from ... 4 6 18.0% 0.00307 39566
[View Peptides]   KTR6 Probable mannosylphosphate transferase involved in the synthesis of core oligosaccharides in protein... 5 7 17.7% 0.0029 52128
[View Peptides]   VMA6 Subunit d of the five-subunit V0 integral membrane domain of vacuolar H+-ATPase (V-ATPase), an elect... 4 8 17.4% 0.00429 39791
[View Peptides]   PIL1 Primary component of eisosomes, which are large immobile cell cortex structures associated with endo... 4 5 17.1% 0.00273 38349
[View Peptides]   NYV1 v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent C... 4 13 17.0% 0.0095 28964
[View Peptides]   PHO8 Repressible alkaline phosphatase, a glycoprotein localized to the vacuole; regulated by levels of in... 10 29 16.3% 0.00947 63004
[View Peptides]   NPL3 RNA-binding protein that promotes elongation, regulates termination, and carries poly(A) mRNA from n... 5 7 16.2% 0.00313 45407
[View Peptides]   SHM2 Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrof... 6 16 16.0% 0.00631 52219
[View Peptides]   LPD1 Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydroge... 5 7 15.6% 0.00259 54010
[View Peptides]   ILV6 Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino ... 3 3 14.9% 0.00179 33987
[View Peptides]   VTC4 Vacuolar membrane polyphosphate polymerase; subunit of the vacuolar transporter chaperone (VTC) comp... 9 15 14.7% 0.00385 83155
[View Peptides]   PEP12 Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus... 3 4 14.6% 0.00257 33006
[View Peptides]   YPK9 Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-t... 16 26 14.6% 0.00326 166749
[View Peptides]   PDX1 Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogena... 4 10 14.6% 0.00451 45362
[View Peptides]   ERV25 Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family in... 2 2 13.7% 0.00175 24106
[View Peptides]   YBT1 Transporter of the ATP-binding cassette (ABC) family involved in bile acid transport; similar to mam... 18 27 13.5% 0.003 189161
[View Peptides]   FRA1 Protein involved in negative regulation of transcription of iron regulon; forms an iron independent ... 8 16 13.4% 0.00395 84924
[View Peptides]   YDR089W Protein of unknown function; deletion confers resistance to Nickel 8 13 13.2% 0.00277 98712
[View Peptides]   GRX6 Cis-golgi localized monothiol glutaredoxin that binds an iron-sulfur cluster; more similar in activi... 2 2 13.0% 0.0016 25783
[View Peptides]   TLG1 Essential t-SNARE that forms a complex with Tlg2p and Vti1p and mediates fusion of endosome-derived ... 2 5 12.9% 0.00413 25817
[View Peptides]   FKS1 Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... 17 29 12.7% 0.00286 214849
[View Peptides]   RPP0 Conserved ribosomal protein P0 similar to rat P0, human P0, and E. coli L10e; shown to be phosphoryl... 3 6 12.5% 0.00355 33717
[View Peptides]   SAC1 Phosphatidylinositol phosphate (PtdInsP) phosphatase involved in hydrolysis of PtdIns[4]P; transmemb... 5 5 12.4% 0.00148 71125
[View Peptides]   PHO84 High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated... 7 14 12.3% 0.00441 64382
[View Peptides]   BGL2 Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance 3 3 12.1% 0.00177 34119
[View Peptides]   CCC1 Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack ... 3 4 12.1% 0.0023 34250
[View Peptides]   GRX7 Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol... 2 2 11.8% 0.00182 22565
[View Peptides]   ERG2 C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position... 2 2 11.7% 0.00167 24896
[View Peptides]   SEC4 Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with t... 2 4 11.6% 0.00344 23506
[View Peptides]   CPS1 Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions 5 10 11.3% 0.00321 64597
[View Peptides]   YPT6 Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusi... 2 2 11.2% 0.00172 24413
[View Peptides]   DBP2 Essential ATP-dependent RNA helicase of the DEAD-box protein family, involved in nonsense-mediated m... 5 6 11.2% 0.00203 60999
[View Peptides]   HMX1 ER localized, heme-binding peroxidase involved in the degradation of heme; does not exhibit heme oxy... 2 3 11.0% 0.00175 37179
[View Peptides]   TFP1 Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein prec... 9 15 10.9% 0.00259 118637
[View Peptides]   PRM5 Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrit... 3 5 10.7% 0.00291 34651
[View Peptides]   PMA1 Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... 6 7 10.7% 0.00141 99619
[View Peptides]   NOP56 Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that ... 3 6 10.5% 0.0022 56864
[View Peptides]   PHO90 Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthet... 6 11 10.3% 0.00231 97689
[View Peptides]   YCF1 Vacuolar glutathione S-conjugate transporter of the ATP-binding cassette family, has a role in detox... 11 21 10.2% 0.00256 171120
[View Peptides]   VAM6 Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by fa... 7 13 10.1% 0.00229 122881
[View Peptides]   HMG1 One of two isozymes of HMG-CoA reductase that catalyzes the conversion of HMG-CoA to mevalonate, whi... 7 13 9.6% 0.00228 115625
[View Peptides]   VOA1 Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of t... 2 2 9.4% 0.0014 29678
[View Peptides]   GSF2 ER localized integral membrane protein that may promote secretion of certain hexose transporters, in... 2 3 9.4% 0.00138 45870
[View Peptides]   NOP58 Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the ... 3 3 9.4% 0.00109 56956
[View Peptides]   VTC3 Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuol... 6 12 9.3% 0.00266 96553
[View Peptides]   ADE5,7 Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole... 5 7 9.2% 0.00161 86068
[View Peptides]   MNN1 Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for add... 6 10 9.1% 0.00243 88529
[View Peptides]   YNL217W Hypothetical protein 2 2 8.9% 0.00113 37151
[View Peptides]   YME1 Catalytic subunit of the mitochondrial inner membrane i-AAA protease complex, which is responsible f... 5 5 8.8% 0.00124 81772
[View Peptides]   URA2 Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes t... 13 16 8.7% 0.00134 245123
[View Peptides]   KRE2 Alpha1,2-mannosyltransferase of the Golgi involved in protein mannosylation 3 3 8.4% 0.00125 51387
[View Peptides]   HXT3 Low affinity glucose transporter of the major facilitator superfamily, expression is induced in low ... 3 4 8.3% 0.0013 62558
[View Peptides]   PGA3 Putative cytochrome b5 reductase, localized to the plasma membrane; may be involved in regulation of... 2 2 8.0% 0.00118 35287
[View Peptides]   COS6 Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-enc... 2 4 7.9% 0.00194 45678
[View Peptides]   VAN1 Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first st... 2 4 7.9% 0.00138 61092
[View Peptides]   PGK1 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phospha... 2 2 7.7% 8.89E-4 44738
[View Peptides]   VPS16 Subunit of the vacuole fusion and protein sorting HOPS complex and the CORVET tethering complex; par... 4 4 7.5% 9.27E-4 92741
[View Peptides]   CPR8 Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide ... 2 2 7.1% 0.0012 34946
[View Peptides]   YDL180W Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to th... 2 3 7.1% 0.00101 62288
[View Peptides]   HIP1 High-affinity histidine permease, also involved in the transport of manganese ions 3 3 7.1% 9.2E-4 66006
[View Peptides]   TRM8 Subunit of a tRNA methyltransferase complex composed of Trm8p and Trm82p that catalyzes 7-methylguan... 2 2 7.0% 0.00129 33391
[View Peptides]   KTR3 Putative alpha-1,2-mannosyltransferase involved in O- and N-linked protein glycosylation; member of ... 2 2 6.9% 9.15E-4 47482
[View Peptides]   KRE6 Protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; appears functional... 4 4 6.9% 0.00103 80123
[View Peptides]   PDI1 Protein disulfide isomerase, multifunctional protein resident in the endoplasmic reticulum lumen, es... 2 8 6.7% 0.00283 58227
[View Peptides]   PEP5 Component of CORVET tethering complex; peripheral vacuolar membrane protein required for protein tra... 3 5 6.7% 8.98E-4 117477
[View Peptides]   VAC8 Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for ... 3 6 6.6% 0.00192 63208
[View Peptides]   SEC63 Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/Bi... 3 3 6.6% 8.36E-4 75345
[View Peptides]   TLG2 Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-deri... 2 2 6.5% 9.31E-4 45876
[View Peptides]   VPS41 Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sort... 4 12 6.4% 0.00224 113412
[View Peptides]   ZRC1 Vacuolar membrane zinc transporter, transports zinc from the cytosol into the vacuole for storage; a... 2 6 6.3% 0.00251 48345
[View Peptides]   GNT1 N-acetylglucosaminyltransferase capable of modification of N-linked glycans in the Golgi apparatus 2 3 6.3% 0.00113 57578
[View Peptides]   YCK3 Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fus... 2 5 6.3% 0.00176 60261
[View Peptides]   FAA4 Long chain fatty acyl-CoA synthetase, activates imported fatty acids with a preference for C12:0-C16... 3 4 6.3% 0.00107 77267
[View Peptides]   YBR287W Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR... 2 2 6.1% 8.66E-4 47506
[View Peptides]   ENA1 P-type ATPase sodium pump, involved in Na+ and Li+ efflux to allow salt tolerance 4 6 6.0% 0.00102 120357
[View Peptides]   SKG6 Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell ... 3 5 6.0% 0.00126 81849
[View Peptides]   ENA2 P-type ATPase sodium pump, involved in Na+ efflux to allow salt tolerance; likely not involved in Li... 4 6 6.0% 0.00102 120317
[View Peptides]   ENA5 Protein with similarity to P-type ATPase sodium pumps, member of the Na+ efflux ATPase family 4 6 6.0% 0.00102 120296
[View Peptides]   LYP1 Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of c... 3 3 5.9% 9.08E-4 68090
[View Peptides]   MNR2 Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has si... 4 5 5.9% 9.54E-4 109736
[View Peptides]   VMA2 Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... 2 3 5.8% 0.00107 57749
[View Peptides]   TEF1, TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... 3 4 5.7% 0.00161 50033
[View Peptides]   DED1 ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all ye... 2 3 5.6% 9.18E-4 65553
[View Peptides]   TUB2 Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymeriz... 2 2 5.5% 8.09E-4 50923
[View Peptides]   FPS1 Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glyce... 2 2 5.5% 5.53E-4 73877
[View Peptides]   HSP60 Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides... 2 2 5.1% 6.46E-4 60752
[View Peptides]   BPT1 ABC type transmembrane transporter of MRP/CFTR family, found in vacuolar membrane, involved in the t... 5 5 4.9% 5.93E-4 176873
[View Peptides]   PEX3 Peroxisomal membrane protein (PMP) required for proper localization and stability of PMPs; anchors p... 2 2 4.8% 8.38E-4 50676
[View Peptides]   MAM3 Protein required for normal mitochondrial morphology, has similarity to hemolysins 3 3 4.7% 7.85E-4 77712
[View Peptides]   CHS2 Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of ... 3 3 4.7% 5.76E-4 109882
[View Peptides]   YER138C Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 6 10 4.7% 0.00105 198561
[View Peptides]   LAP4 Vacuolar aminopeptidase yscI; zinc metalloproteinase that belongs to the peptidase family M18; often... 2 3 4.5% 0.00108 57093
[View Peptides]   SHE10 Putative glycosylphosphatidylinositol (GPI)-anchored protein of unknown function; overexpression cau... 2 2 4.5% 6.41E-4 66862
[View Peptides]   SLT2 Serine/threonine MAP kinase involved in regulating the maintenance of cell wall integrity and progre... 2 2 4.3% 7.64E-4 55636
[View Peptides]   VBA4 Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GF... 2 2 4.3% 4.81E-4 85683
[View Peptides]   GNP1 High-affinity glutamine permease, also transports Leu, Ser, Thr, Cys, Met and Asn; expression is ful... 2 4 4.2% 0.00112 73598
[View Peptides]   PEP3 Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular ... 3 5 4.2% 0.00101 107398
[View Peptides]   PHO91 Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90,... 4 4 4.1% 8.27E-4 99490
[View Peptides]   FET5 Multicopper oxidase, integral membrane protein with similarity to Fet3p; may have a role in iron tra... 2 4 4.0% 0.00119 70880
[View Peptides]   VTC2 Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuol... 3 6 3.9% 0.00134 95441
[View Peptides]   YVC1 Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic sho... 2 2 3.7% 5.48E-4 77953
[View Peptides]   VPS33 ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essen... 2 2 3.6% 5.35E-4 79271
[View Peptides]   PMR1 High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in C... 2 2 3.6% 3.89E-4 104571
[View Peptides]   VAC7 Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p k... 4 5 3.6% 7.93E-4 128140
[View Peptides]   CHS3 Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for ... 2 2 3.4% 3.17E-4 131601
[View Peptides]   PEP1 Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golg... 3 3 3.2% 3.51E-4 177777
[View Peptides]   CCC2 Cu(+2)-transporting P-type ATPase, required for export of copper from the cytosol into an extracytos... 2 2 3.1% 3.68E-4 109829
[View Peptides]   CHS1 Chitin synthase I, requires activation from zymogenic form in order to catalyze the transfer of N-ac... 2 2 3.1% 3.27E-4 129919
[View Peptides]   YGR125W Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal ... 2 2 3.0% 3.57E-4 116971
[View Peptides]   YLR241W Putative protein of unknown function, may be involved in detoxification 2 4 2.9% 9.45E-4 89344
[View Peptides]   PMC1 Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activ... 2 2 2.0% 3.15E-4 130861
YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle