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Protein Overview: VAC8

General Information:
Name(s) found: VAC8 / YEL013W [SGD]
Description(s) found:
Found 25 descriptions.
SHOW ALL
  • Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 578 amino acids

    Gene Ontology:
    Cellular Component: fungal-type vacuole [IDALink to PubMed article]
    Biological Process: vacuole inheritance [IMPLink to PubMed article]
    vacuole fusion, non-autophagic [IDALink to PubMed article][IMPLink to PubMed article]
    protein targeting to vacuole [IMPLink to PubMed article]
    microautophagy [IMPLink to PubMed article]
    Molecular Function: protein binding [IPILink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MGSCCSCLKD SSDEASVSPI ADNEREAVTL LLGYLEDKDQ LDFYSGGPLK ALTTLVYSDN  60
   61 LNLQRSAALA FAEITEKYVR QVSREVLEPI LILLQSQDPQ IQVAACAALG NLAVNNENKL 120
  121 LIVEMGGLEP LINQMMGDNV EVQCNAVGCI TNLATRDDNK HKIATSGALI PLTKLAKSKH 180
  181 IRVQRNATGA LLNMTHSEEN RKELVNAGAV PVLVSLLSST DPDVQYYCTT ALSNIAVDEA 240
  241 NRKKLAQTEP RLVSKLVSLM DSPSSRVKCQ ATLALRNLAS DTSYQLEIVR AGGLPHLVKL 300
  301 IQSDSIPLVL ASVACIRNIS IHPLNEGLIV DAGFLKPLVR LLDYKDSEEI QCHAVSTLRN 360
  361 LAASSEKNRK EFFESGAVEK CKELALDSPV SVQSEISACF AILALADVSK LDLLEANILD 420
  421 ALIPMTFSQN QEVSGNAAAA LANLCSRVNN YTKIIEAWDR PNEGIRGFLI RFLKSDYATF 480
  481 EHIALWTILQ LLESHNDKVE DLVKNDDDII NGVRKMADVT FERLQRSGID VKNPGSNNNP 540
  541 SSNDNNSNNN DTGSEHQPVE DASLELYNIT QQILQFLH

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Krogan NJ, et al. (2006) FMP41 VAC8
    View Details Ho Y, et al. (2002) ATP3 BRR2 FAA4 FET3 GCD1 GCD11 GCD2 GCD6 GCD7 GCN3 LOS1 MET18 NMD5 PRO3 RFC2 RFC3 RFC4 ROM2 SCW4 SUI3 VAC8 VMA8

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run VAM3 Sample: HX11 - both fusion and trans-SNARE complex formation take place. Hao Xu, et al. (2010)
    View Run VAM3 Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control). Hao Xu, et al. (2010)
    View Run VAM3 Sample: HX14 - mixture in detergent (control). Hao Xu, et al. (2010)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data VAC8 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..86] PSI-BLAST 123.0 beta-Catenin
    2 View Details [87..204] PSI-BLAST 123.0 beta-Catenin
    3 View Details [205..293] PSI-BLAST 123.0 beta-Catenin
    4 View Details [294..334] PSI-BLAST 123.0 beta-Catenin
    5 View Details [335..441] PSI-BLAST 123.0 beta-Catenin
    6 View Details [442..578] PSI-BLAST 194.28043 Karyopherin alpha

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • protein transporter activity
    		•  3.09219467906677 bayes_pls_golite062009
  • binding
    		•  2.48958374349477 bayes_pls_golite062009
  • transporter activity
    		•  2.48700774379895 bayes_pls_golite062009
  • substrate-specific transporter activity
    		•  2.01243877392722 bayes_pls_golite062009
  • protein binding
    		•  1.94427330399472 bayes_pls_golite062009
  • clathrin binding
    		•  1.82663703481803 bayes_pls_golite062009
  • transmembrane transporter activity
    		•  1.28481004323799 bayes_pls_golite062009
  • protein transmembrane transporter activity
    		•  1.27438167414302 bayes_pls_golite062009
  • substrate-specific transmembrane transporter activity
    		•  0.851483195437252 bayes_pls_golite062009
  • nuclear localization sequence binding
    		•  0.661470469730249 bayes_pls_golite062009
  • hydrolase activity
    		•  0.610187344532862 bayes_pls_golite062009
  • signal sequence binding
    		•  0.57106697498903 bayes_pls_golite062009
  • active transmembrane transporter activity
    		•  0.252495966234234 bayes_pls_golite062009
  • primary active transmembrane transporter activity
    		•  0.00492507437049428 bayes_pls_golite062009
    2 No functions predicted.
    3 No functions predicted.
    4 No functions predicted.
    5 No functions predicted.
    6 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle