Protein Overview: VMA8

General Information:
Name(s) found:	VMA8 / YEL051W [SGD]
Description(s) found: Found 29 descriptions. SHOW ALL	Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis [SGD]
Organism:	Saccharomyces cerevisiae
Length:	256 amino acids
Gene Ontology:
Cellular Component:	vacuolar proton-transporting V-type ATPase, V1 domain [TAS ] fungal-type vacuole membrane [TAS]
Biological Process:	vacuolar acidification [TAS ]
Molecular Function:	proton-transporting ATPase activity, rotational mechanism [TAS ]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSGNREQVFP TRMTLGLMKT KLKGANQGYS LLKRKSEALT KRFRDITKRI DDAKQKMGRV  60
   61 MQTAAFSLAE VSYATGENIG YQVQESVSTA RFKVRARQEN VSGVYLSQFE SYIDPEINDF 120
  121 RLTGLGRGGQ QVQRAKEIYS RAVETLVELA SLQTAFIILD EVIKVTNRRV NAIEHVIIPR 180
  181 TENTIAYINS ELDELDREEF YRLKKVQEKK QNETAKLDAE MKLKRDRAEQ DASEVAADEE 240
  241 PQGETLVADQ EDDVIF

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ATP3 TFP1 VMA10 VMA13 VMA4 VMA7 VMA8
View Details	(MIPS) Mewes HW, et al. (2004)		CUP5 PPA1 STV1 TFP1 TFP3 VMA10 VMA13 VMA2 VMA4 VMA5 VMA6 VMA7 VMA8 VPH1
View Details	Krogan NJ, et al. (2006)		CLG1 CUE1 GTR1 HAM1 HRT1 LAP3 TFP1 VMA10 VMA13 VMA2 VMA7 VMA8 XDJ1 YIG1
View Details	Gavin AC, et al. (2006)		VMA13 VMA4 VMA5 VMA7 VMA8
View Details	Gavin AC, et al. (2002)		RAV1 TFP1 VMA10 VMA13 VMA2 VMA4 VMA5 VMA6 VMA7 VMA8 VPH1
View Details	Ho Y, et al. (2002)		AAC3 ADR1 AHA1 ARP2 ATP3 AVO1 AYR1 BEM2 BGL2 CBP6 CCT5 CLU1 CPR6 DHH1 DPB2 ECM10 ENP1 FAA1 FAA4 FET3 FUN12 GAL7 GAR1 GCD1 GCD11 GCD2 GCD6 GCD7 GCN3 GPH1 GSP1 GUF1 HEM15 HOM3 HOR2 HRP1 IDH1 ILV2 KAP104 KTR3 LOS1 MAM33 MET16 MET18 MKK2 MKT1 MRE11 MRT4 NAB2 NDE1 NMD5 NOG2 NPA3 OAC1 OYE2 PDX1 PET10 PET9 PHO84 PHO85 PIL1 PPH21 PPH22 PRB1 PRO3 PSD1 PTC3 PUF3 RAD50 RAD52 RAD59 REP1 RHR2 RPN12 RPN8 RPT4 SCW4 SDH2 SEC27 SEC53 SIS1 SLC1 SLX1 SPS1 SRP54 STI1 SUI3 TEF4 TEM1 TFC7 TFG2 UBA1 VAC8 VMA8 VPS74 XRS2 YDL063C YER077C YER138C YGR266W YHM2 YHR033W YKR051W YKU80 YLR271W YNL035C YPR003C YPT31
View Details	Qiu et al. (2008)		CUP5 PPA1 STV1 TFP1 TFP3 VMA10 VMA13 VMA2 VMA4 VMA5 VMA6 VMA7 VMA8 VMA9 VPH1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	MLP2	#25 Asynchronous Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	STE50	VMA8	2	Unpublished Fields Lab Data
View Screen	YDR132C	VMA8	2	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	VMA8	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..256]	ORFEUS	7.56	Apolipoprotein A-I

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2