Protein Overview: KAP104

General Information:
Name(s) found:	KAP104 / YBR017C [SGD]
Description(s) found: Found 28 descriptions. SHOW ALL	Transportin or cytosolic karyopherin beta 2; functions in the rg-nuclear localization signal-mediated nuclear import/reimport of mRNA-binding proteins Nab2p and Hrp1p; regulates asymmetric protein synthesis in daughter cells during mitosis [SGD]
Organism:	Saccharomyces cerevisiae
Length:	918 amino acids
Gene Ontology:
Cellular Component:	cytosol [IDA]
Biological Process:	mRNA-binding (hnRNP) protein import into nucleus [IDA][IMP][IPI][ISS] protein import into nucleus [IDA][IMP][IPI][ISS] cell cycle [IMP]
Molecular Function:	nuclear localization sequence binding [IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MASTWKPAED YVLQLATLLQ NCMSPNPEIR NNAMEAMENF QLQPEFLNYL CYILIEGESD  60
   61 DVLKQHYSLQ DLQNNRATAG MLLKNSMLGG NNLIKSNSHD LGYVKSNIIH GLYNSNNNLV 120
  121 SNVTGIVITT LFSTYYRQHR DDPTGLQMLY QLLELTSNGN EPSIKALSKI MEDSAQFFQL 180
  181 EWSGNTKPME ALLDSFFRFI SNPNFSPVIR SESVKCINTV IPLQTQSFIV RLDKFLEIIF 240
  241 QLAQNDENDL VRAQICISFS FLLEFRPDKL VSHLDGIVQF MLHLITTVNE EKVAIEACEF 300
  301 LHAFATSPNI PEHILQPYVK DIVPILLSKM VYNEESIVLL EASNDDDAFL EDKDEDIKPI 360
  361 APRIVKKKEA GNGEDADDNE DDDDDDDDED GDVDTQWNLR KCSAATLDVM TNILPHQVMD 420
  421 IAFPFLREHL GSDRWFIREA TILALGAMAE GGMKYFNDGL PALIPFLVEQ LNDKWAPVRK 480
  481 MTCWTLSRFS PWILQDHTEF LIPVLEPIIN TLMDKKKDVQ EAAISSVAVF IENADSELVE 540
  541 TLFYSQLLTS FDKCLKYYKK KNLIILYDAI GRFAEKCALD ETAMQIILPP LIEKWALLSD 600
  601 SDKELWPLLE CLSCVASSLG ERFMPMAPEV YNRAFRILCH CVELEAKSHQ DPTIVVPEKD 660
  661 FIITSLDLID GLVQGLGAHS QDLLFPQGTK DLTILKIMLE CLQDPVHEVR QSCFALLGDI 720
  721 VYFFNSELVI GNLEDFLKLI GTEIMHNDDS DGTPAVINAI WALGLISERI DLNTYIIDMS 780
  781 RIILDLFTTN TQIVDSSVME NLSVTIGKMG LTHPEVFSSG AFANDSNWNK WCLSVNALDD 840
  841 VEEKSSAYMG FLKIINLTST EVTMSNDTIH KIVTGLSSNV EANVFAQEIY TFLMNHSAQI 900
  901 SAINFTPDEI SFLQQFTS

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ENP1 GEA2 HRP1 KAP104 KTR3 MIR1 NAB2 YHR020W YNL035C
View Details	Krogan NJ, et al. (2006)		ALD4 KAP104 TAF6 TDA5
View Details	Gavin AC, et al. (2006)		CLU1 HRP1 KAP104
View Details	Gavin AC, et al. (2002)		BMH1 BMH2 CLU1 CRM1 ECM29 FKS1 GCN1 GEA2 GFA1 HRP1 KAP104 KAP123 LCB1 LCB2 LYS12 MIR1 NAB2 SAM1 SAM2 YHR020W
View Details	Ho Y, et al. (2002)		ADH4 ADR1 ARC1 AYR1 BGL2 CBP6 DIM1 DPS1 ENP1 FAA1 GAR1 GSP1 GSP2 HEM15 HRP1 HTA2 KAP104 KTR3 MRT4 NAB2 NDE1 OAC1 PAA1 PET10 PET9 PGM2 PSD1 RPT4 RTN2 SAC1 TFG2 VMA8 YDL063C YHM2 YNL035C YRB1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	KAP104	PRP45	2	Unpublished Fields Lab Data
View Screen	KAP104	SIP2	2	Unpublished Fields Lab Data
View Screen	KAP104	BSC5	2	Uetz P, et al. (2000)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	KAP104	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..96]	deduced	N/A	No confident structure predictions are available.
2	View Details	[97..688]	MSA	20.431997	View MSA. No confident structure predictions are available.
3	View Details	[689..858]	MSA	2.262988	View MSA. Confident ab initio structure predictions are available.
4	View Details	[859..918]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
protein transporter activity	4.58709163830613	bayes_pls_golite062009
binding	3.0667965351474	bayes_pls_golite062009
protein transmembrane transporter activity	2.74547353635661	bayes_pls_golite062009
transporter activity	2.48700774379895	bayes_pls_golite062009
nucleic acid binding	2.34284977509828	bayes_pls_golite062009
substrate-specific transporter activity	2.26725422172281	bayes_pls_golite062009
nuclear localization sequence binding	1.91487015967262	bayes_pls_golite062009
transcription regulator activity	1.76231475025891	bayes_pls_golite062009
protein binding	1.73688475473996	bayes_pls_golite062009
DNA binding	1.62032607987786	bayes_pls_golite062009
nuclear export signal receptor activity	1.29390474721722	bayes_pls_golite062009
transmembrane transporter activity	1.28481004323799	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	1.09652988954158	bayes_pls_golite062009
transcription factor activity	0.923253759652909	bayes_pls_golite062009
structural molecule activity	0.908029258648777	bayes_pls_golite062009
translation initiation factor activity	0.83079633551839	bayes_pls_golite062009
translation regulator activity	0.82193201542158	bayes_pls_golite062009
translation factor activity, nucleic acid binding	0.803700928110921	bayes_pls_golite062009
molecular transducer activity	0.662662274021266	bayes_pls_golite062009
signal transducer activity	0.662662274021266	bayes_pls_golite062009
signal sequence binding	0.57106697498903	bayes_pls_golite062009
mRNA binding	0.46864854653143	bayes_pls_golite062009
RNA binding	0.40024158562931	bayes_pls_golite062009
clathrin binding	0.35775052108553	bayes_pls_golite062009
active transmembrane transporter activity	0.252495966234234	bayes_pls_golite062009
ion transmembrane transporter activity	0.248585912061178	bayes_pls_golite062009
primary active transmembrane transporter activity	0.226380819487924	bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity	0.217517113570989	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	0.178770060806735	bayes_pls_golite062009
ATPase activity, coupled to movement of substances	0.175241020572885	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of substances	0.174922130116569	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.100181463251306	bayes_pls_golite062009
pyrophosphatase activity	0.0635404099346928	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.0548082069333885	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.052195223119721	bayes_pls_golite062009
cation transmembrane transporter activity	0.0405268469927988	bayes_pls_golite062009
transcription activator activity	0.0232727461674547	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.96

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2