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Protein Overview: SEC27

General Information:
Name(s) found: SEC27 / YGL137W [SGD]
Description(s) found:
Found 27 descriptions.
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  • Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 889 amino acids

    Gene Ontology:
    Cellular Component: COPI vesicle coat [IGILink to PubMed article][ISSLink to PubMed article]
    Biological Process: ER to Golgi vesicle-mediated transport [IMPLink to PubMed article]
    retrograde vesicle-mediated transport, Golgi to ER [IMPLink to PubMed article]
    Molecular Function: molecular_function [ND]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKLDIKKTFS NRSDRVKGID FHPTEPWVLT TLYSGRVELW NYETQVEVRS IQVTETPVRA  60
   61 GKFIARKNWI IVGSDDFRIR VFNYNTGEKV VDFEAHPDYI RSIAVHPTKP YVLSGSDDLT 120
  121 VKLWNWENNW ALEQTFEGHE HFVMCVAFNP KDPSTFASGC LDRTVKVWSL GQSTPNFTLT 180
  181 TGQERGVNYV DYYPLPDKPY MITASDDLTI KIWDYQTKSC VATLEGHMSN VSFAVFHPTL 240
  241 PIIISGSEDG TLKIWNSSTY KVEKTLNVGL ERSWCIATHP TGRKNYIASG FDNGFTVLSL 300
  301 GNDEPTLSLD PVGKLVWSGG KNAAASDIFT AVIRGNEEVE QDEPLSLQTK ELGSVDVFPQ 360
  361 SLAHSPNGRF VTVVGDGEYV IYTALAWRNK AFGKCQDFVW GPDSNSYALI DETGQIKYYK 420
  421 NFKEVTSWSV PMHSAIDRLF SGALLGVKSD GFVYFFDWDN GTLVRRIDVN AKDVIWSDNG 480
  481 ELVMIVNTNS NGDEASGYTL LFNKDAYLEA ANNGNIDDSE GVDEAFDVLY ELSESITSGK 540
  541 WVGDVFIFTT ATNRLNYFVG GKTYNLAHYT KEMYLLGYLA RDNKVYLADR EVHVYGYEIS 600
  601 LEVLEFQTLT LRGEIEEAIE NVLPNVEGKD SLTKIARFLE GQEYYEEALN ISPDQDQKFE 660
  661 LALKVGQLTL ARDLLTDESA EMKWRALGDA SLQRFNFKLA VEAFTNAHDL ESLFLLHSSF 720
  721 NNKEGLVTLA KDAERAGKFN LAFNAYWIAG DIQGAKDLLI KSQRFSEAAF LGSTYGLGDD 780
  781 AVNDIVTKWK ENLILNGKNT VSERVCGAEG LPGSSSSGDA QPLIDLDSTP APEQADENKE 840
  841 AEVEDSEFKE SNSEAVEAEK KEEEAPQQQQ SEQQPEQGEA VPEPVEEES

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) COP1 EFT2, EFT1 GLO3 RET2 RET3 SEC21 SEC26 SEC27 SEC28
    View Details (MIPS) Mewes HW, et al. (2004) ARF1 COP1 RET2 RET3 SEC21 SEC26 SEC27 SEC28
    View Details Krogan NJ, et al. (2006) COP1 FCF2 FMP23 LCB5 PIP2 RET2 RET3 SCS2 SEC2 SEC21 SEC26 SEC27 SEC28
    View Details Gavin AC, et al. (2006) COP1 RET2 RET3 SEC21 SEC26 SEC27 SEC28
    View Details Gavin AC, et al. (2002) ACC1 AHA1 ATG26 ATP11 BEM2 BUD3 CAF130 CAF40 CAM1 CCR4 CCT6 CCT8 CDC36 CDC39 CLU1 COP1 CPA2 CYR1 EFB1 FAP1 FAS2 FDH1 GCN1 GCN20 GFA1 GLO3 GPT2 GUS1 HRR25 ILV1 IMG1 IMG2 IML1 KAP123 LYS12 MGA2 MHR1 MOT2 MRH4 MRM1 MRP20 MRP49 MRP7 MRPL1 MRPL10 MRPL13 MRPL16 MRPL17 MRPL19 MRPL20 MRPL23 MRPL24 MRPL25 MRPL27 MRPL28 MRPL3 MRPL35 MRPL36 MRPL39 MRPL4 MRPL44 MRPL51 MRPL6 MRPL7 MRPL8 NEW1 NOT3 NOT5 NUG1 OLA1 PDC1 PGK1 POL1 POL12 POP2 PRI1 PRI2 PRP43 PSA1 RET2 RET3 ROM2 RPA135 RPB2 RPN10 RPN11 RPN12 RPN8 RPT3 RPT5 RPT6 RTC6 RVB2 SAM1 SBP1 SCJ1 SEC1 SEC21 SEC26 SEC27 SEC28 SEH1 SIN4 SMC3 SNF2 SWI3 SYP1 TAF5 TCB1 TEF1, TEF2 TEF4 TFC7 TFP1 TIF1, TIF2 TOS1 TSA1 TUB3 URA7 UTP20 VAS1 YDR115W YML6 YOL098C YRA1
    View Details Ho Y, et al. (2002) AAC3 ACC1 ACH1 ADE5,7 ADH2 ADK1 ADR1 AHA1 AHP1 ARF1 ARG4 ATP3 AYR1 BCY1 BEM2 BFR2 BIR1 BNA5 BTN2 CCT2 CCT6 CDC53 CDC7 CLU1 COP1 COR1 CPR6 CTR1 CYR1 DBF2 DHH1 DHR2 DUN1 EAP1 ECM10 ERG27 FAA1 FMP40 GAL7 GCD11 GDT1 GFA1 GND1 GPG1 GPH1 GPT2 GUF1 GUS1 HEF3 HOM3 HOR2 HRT1 HTB1 IDH1 IDH2 ILV2 ISA2 JIP3 JJJ1 LHS1 LIA1 MAE1 MDH1 MEH1 MET18 MGE1 MGT1 MOB1 MRE11 NAN1 NGL2 NOP1 NPA3 NTG1 NUT1 OM45 OPY1 OYE2 PCT1 PDA1 PDC5 PDC6 PET10 PET9 PFK1 PGM1 PGM2 PHO86 PIL1 PRB1 PRE10 PRO3 PRP11 PST2 PTC3 PYC1 PYC2 RAD50 RAD52 RAD59 RET1 RET2 REX2 RHR2 RIP1 RIX7 RNR2 RPA135 RPA190 RPA49 RPB3 RPB5 RPC19 RPC25 RPC34 RPC40 RPC82 RPN3 RPN5 RPN7 RPN8 RPO26 RPO31 RPT3 RPT5 RTN2 RTT101 RVS161 RVS167 SAR1 SEC18 SEC21 SEC26 SEC27 SEC28 SEC53 SHM2 SOF1 SSK2 STI1 TBS1 TCP1 TEF4 TFP1 THI22 THI3 TIF34 TIF35 TIF4631 TMA19 TPA1 TPK1 TPK3 TPS1 TRP3 TSA2 UBA1 UBI4 UBP15 UBR1 UTP13 VAC14 VMA8 XRS2 YCR076C YER138C YFL042C YGR250C YHR033W YHR112C YHR214W-A YNL181W YPT31 YPT7
    View Details Qiu et al. (2008) ARF1 COP1 GLO3 RER1 RET2 RET3 SAR1 SEC13 SEC21 SEC26 SEC27 SEC28 VRG4

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run RPA135 his-HA tag on RPA135 Schneider, DA, et al. (2006)
    View Run CEP3 sample cbf3 from feb 2005 Sandall S, et all (2006)
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run ZDS1 Sample 2- cpn2 from june 2005 McCusker D, et al (2007)
    View Run ZDS1 Sample 3 - cpn + atp, from june 2005 McCusker D, et al (2007)
    View Run BOI1 Sample boi 1 with ha tag from october 2005 McCusker D, et al (2007)
    View Run VAM3 Sample: HX13 - trans-SNARE complex forms but fusion is blocked. Hao Xu, et al. (2010)
    View Run MLP2 #31 Asynchronous Prep (Protease cleavage) Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data SEC27 and ANP1 Huh WK, et al. (2003)
    View Data SEC27 Huh WK, et al. (2003)
    View Data SEC27 and COP1 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..311] PSI-BLAST 219.68867 Tup1, C-terminal domain
    2 View Details [312..377] MSA 3.106993 View MSA. No confident structure predictions are available.
    3 View Details [378..629] PSI-BLAST 3.0 TolB, C-terminal domain; TolB, N-terminal domain
    4 View Details [630..812] ORFEUS 10.2 Clathrin heavy chain proximal leg segment
    5 View Details [813..889] MSA 1.023991 View MSA. No confident structure predictions are available.

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2 No functions predicted.
    3
    Term Confidence Notes
  • histone methyltransferase activity (H3-K4 specific)
    		•  4.71992275913384 bayes_pls_golite062009
  • actin binding
    		•  3.31804728298431 bayes_pls_golite062009
  • S-adenosylmethionine-dependent methyltransferase activity
    		•  3.25521258308263 bayes_pls_golite062009
  • cytoskeletal protein binding
    		•  3.1056443224755 bayes_pls_golite062009
  • tubulin binding
    		•  2.76945430061825 bayes_pls_golite062009
  • microtubule binding
    		•  2.72684103222924 bayes_pls_golite062009
  • dynein binding
    		•  2.54821997913979 bayes_pls_golite062009
  • binding
    		•  2.42570478946173 bayes_pls_golite062009
  • transcription regulator activity
    		•  1.95667720244696 bayes_pls_golite062009
  • nucleic acid binding
    		•  1.90457914882449 bayes_pls_golite062009
  • histone methyltransferase activity
    		•  1.85632145559025 bayes_pls_golite062009
  • dynactin binding
    		•  1.85146647785657 bayes_pls_golite062009
  • actin filament binding
    		•  1.5197243426595 bayes_pls_golite062009
  • structural molecule activity
    		•  1.47448876401574 bayes_pls_golite062009
  • DNA binding
    		•  1.38475369554616 bayes_pls_golite062009
  • protein methyltransferase activity
    		•  1.35170368882651 bayes_pls_golite062009
  • general RNA polymerase II transcription factor activity
    		•  1.22384651674093 bayes_pls_golite062009
  • protein binding
    		•  1.21879977456466 bayes_pls_golite062009
  • transporter activity
    		•  1.21549336905926 bayes_pls_golite062009
  • lysine N-methyltransferase activity
    		•  1.15313682561559 bayes_pls_golite062009
  • protein-lysine N-methyltransferase activity
    		•  1.15313682561559 bayes_pls_golite062009
  • histone-lysine N-methyltransferase activity
    		•  1.15313682561559 bayes_pls_golite062009
  • microtubule plus-end binding
    		•  1.01166033978052 bayes_pls_golite062009
  • protein transporter activity
    		•  0.964340340346761 bayes_pls_golite062009
  • transcription factor activity
    		•  0.804334981068351 bayes_pls_golite062009
  • molecular transducer activity
    		•  0.762533049716683 bayes_pls_golite062009
  • signal transducer activity
    		•  0.762533049716683 bayes_pls_golite062009
  • substrate-specific transporter activity
    		•  0.734793983890004 bayes_pls_golite062009
  • histone binding
    		•  0.685319917508843 bayes_pls_golite062009
  • transcription repressor activity
    		•  0.560226369775279 bayes_pls_golite062009
    		• 0.53274018413176 bayes_pls_golite062009
  • dynein intermediate chain binding
    		•  0.443846546939402 bayes_pls_golite062009
  • phosphotransferase activity, alcohol group as acceptor
    		•  0.426429095658617 bayes_pls_golite062009
  • transcription factor binding
    		•  0.412844855996155 bayes_pls_golite062009
  • kinase activity
    		•  0.387572734234845 bayes_pls_golite062009
  • snoRNA binding
    		•  0.347797037104681 bayes_pls_golite062009
  • protein kinase activity
    		•  0.229242350006544 bayes_pls_golite062009
  • transferase activity, transferring phosphorus-containing groups
    		•  0.226335610368636 bayes_pls_golite062009
  • transcription cofactor activity
    		•  0.19391619547146 bayes_pls_golite062009
  • receptor activity
    		•  0.151162384440161 bayes_pls_golite062009
  • structural constituent of cytoskeleton
    		•  0.118975415284669 bayes_pls_golite062009
  • transcription corepressor activity
    		•  0.00954741955203087 bayes_pls_golite062009
  • nucleoside-triphosphatase activity
    		•  0.00585398682921312 bayes_pls_golite062009
  • N-methyltransferase activity
    		•  0.00335766408304172 bayes_pls_golite062009
    4
    Term Confidence Notes
  • binding
    		•  2.64850159998065 bayes_pls_golite062009
  • transcription regulator activity
    		•  1.96534484578496 bayes_pls_golite062009
  • nucleic acid binding
    		•  1.64804251600372 bayes_pls_golite062009
  • DNA binding
    		•  1.63530462331869 bayes_pls_golite062009
  • protein binding
    		•  1.51322932625387 bayes_pls_golite062009
  • transcription factor activity
    		•  0.941893540415683 bayes_pls_golite062009
  • signal transducer activity
    		•  0.924191975589092 bayes_pls_golite062009
  • molecular transducer activity
    		•  0.924191975589092 bayes_pls_golite062009
  • signal sequence binding
    		•  0.791153798439582 bayes_pls_golite062009
  • N-acetyltransferase activity
    		•  0.597017459591015 bayes_pls_golite062009
  • transcription factor binding
    		•  0.370606597126089 bayes_pls_golite062009
  • N-acyltransferase activity
    		•  0.36489074781643 bayes_pls_golite062009
  • enzyme binding
    		•  0.335436488591595 bayes_pls_golite062009
  • receptor activity
    		•  0.228157571114804 bayes_pls_golite062009
  • peroxisome targeting sequence binding
    		•  0.209148717913838 bayes_pls_golite062009
  • transferase activity
    		•  0.157308242400253 bayes_pls_golite062009
  • transcription cofactor activity
    		•  0.0874081614883346 bayes_pls_golite062009
  • acetyltransferase activity
    		•  0.0217632433282022 bayes_pls_golite062009
    5 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle