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Protein Overview: CDC53

General Information:
Name(s) found: CDC53 / YDL132W [SGD]
Description(s) found:
Found 28 descriptions.
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  • Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 815 amino acids

    Gene Ontology:
    Cellular Component: SCF ubiquitin ligase complex [IDALink to PubMed articleLink to PubMed article][IPILink to PubMed articleLink to PubMed article]
    Biological Process: G2/M transition of mitotic cell cycle [IGILink to PubMed articleLink to PubMed article]
    G1/S transition of mitotic cell cycle [IMPLink to PubMed article]
    SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [IDALink to PubMed articleLink to PubMed article]
    protein ubiquitination during ubiquitin-dependent protein catabolic process [IDALink to PubMed articleLink to PubMed article]
    Molecular Function: DNA replication origin binding [IPILink to PubMed article]
    ubiquitin-protein ligase activity [IDALink to PubMed articleLink to PubMed articleLink to PubMed article]
    protein binding, bridging [IMPLink to PubMed article][IPILink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSETLPRSDD LEATWNFIEP GINQILGNEK NQASTSKRVY KILSPTMYME VYTAIYNYCV  60
   61 NKSRSSGHFS TDSRTGQSTI LVGSEIYEKL KNYLKNYILN FKQSNSETFL QFYVKRWKRF 120
  121 TIGAIFLNHA FDYMNRYWVQ KERSDGKRHI FDVNTLCLMT WKEVMFDPSK DVLINELLDQ 180
  181 VTLGREGQII QRSNISTAIK SLVALGIDPQ DLKKLNLNVY IQVFEKPFLK KTQEYYTQYT 240
  241 NDYLEKHSVT EYIFEAHEII KREEKAMTIY WDDHTKKPLS MALNKVLITD HIEKLENEFV 300
  301 VLLDARDIEK ITSLYALIRR DFTLIPRMAS VFENYVKKTG ENEISSLLAM HKHNIMKNEN 360
  361 ANPKKLALMT AHSLSPKDYI KKLLEVHDIF SKIFNESFPD DIPLAKALDN ACGAFININE 420
  421 FALPAGSPKS ATSKTSEMLA KYSDILLKKA TKPEVASDMS DEDIITIFKY LTDKDAFETH 480
  481 YRRLFAKRLI HGTSTSAEDE ENIIQRLQAA NSMEYTGKIT KMFQDIRLSK ILEDDFAVAL 540
  541 KNEPDYSKAK YPDLQPFVLA ENMWPFSYQE VEFKLPKELV PSHEKLKESY SQKHNGRILK 600
  601 WLWPLCRGEL KADIGKPGRM PFNFTVTLFQ MAILLLYNDA DVLTLENIQE GTSLTIQHIA 660
  661 AAMVPFIKFK LIQQVPPGLD ALVKPETQFK LSRPYKALKT NINFASGVKN DILQSLSGGG 720
  721 HDNHGNKLGN KRLTEDERIE KELNTERQIF LEACIVRIMK AKRNLPHTTL VNECIAQSHQ 780
  781 RFNAKVSMVK RAIDSLIQKG YLQRGDDGES YAYLA

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) CDC34 CDC53 HRT1 MET30 RTG2 RTT101 SAF1 SKP1
    View Details (MIPS) Mewes HW, et al. (2004) CDC34 CDC4 CDC53 GRR1 MET30 SGT1 SKP1
    View Details Krogan NJ, et al. (2006) CAK1 CDC34 CDC53 CDC9 CRT10 GRR1 MEP3 MET30 MMS1 POR1 RTT101 SAF1
    View Details Ho Y, et al. (2002) AAH1 ADH2 ADR1 BBC1 BMS1 BOP2 CDC39 CDC4 CDC53 CIC1 COF1 CPR1 CRM1 CTF4 DAS1 DBP10 DED81 DIA2 DUR1,2 ECM29 ECM33 ENP1 FAA4 FOL2 FPR3 GAL3 GCN1 GRR1 GUF1 HRT1 HRT3 HTB2 HYP2 IDH1 ILS1 KRE33 KRI1 LST4 MCM2 MCM3 MCM5 MDN1 MET30 MET31 MET4 MKT1 MMS1 MYO2 NIP7 NMD3 NOP12 PDC5 PDC6 PFK1 PGM2 PMA2 POR1 PRB1 PTC1 RIX7 RPA190 RPN1 RPN8 RRP12 RTT101 RUB1 SAF1 SAH1 SEC27 SEH1 SGT1 SIS1 SKP1 SLT2 SPB1 SRV2 SSF1 SSF2 SSZ1 STI1 TEF4 THI21 TIF6 TPS1 UBI4 UFO1 URB1 UTP10 VPS13 YAK1 YAR009C YBL104C YBR139W YDR131C YDR306C YGP1 YLR035C-A YLR352W
    View Details Qiu et al. (2008) APC9 CDC34 CDC4 CDC53 DIA2 HRT1 MET30 SKP1 UFO1

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run MLP2 #33 Asynchronous SPB prep Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

      BAIT PREY HITS PUBLICATION
    View Screen CDC53 ERG27 2 Uetz P, et al. (2000)
    View Screen MDM30 CDC53 2 Unpublished Fields Lab Data
    View Screen CDC53 DCN1 2 Uetz P, et al. (2000)
    View Screen CDC53 YDR274C 2 Unpublished Fields Lab Data
    View Screen CDC53 ZIP2 2 Uetz P, et al. (2000)
    View Screen CDC53 SKP1 2 Uetz P, et al. (2000)
    View Screen CDC53 MDM30 2 Uetz P, et al. (2000)
    View Screen CDC53 CDC7 2 Uetz P, et al. (2000)

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data CDC53 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..95]
    [122..155]    		 PSI-BLAST 2110.0 Anaphase promoting complex (APC); Cullin homolog 1, Cul-1; Cullin homolog 1, cul-1
    2 View Details [96..121]
    [156..297]
    [311..324]    		 PSI-BLAST 2110.0 Anaphase promoting complex (APC); Cullin homolog 1, Cul-1; Cullin homolog 1, cul-1
    3 View Details [298..310]
    [325..526]    		 PSI-BLAST 2110.0 Anaphase promoting complex (APC); Cullin homolog 1, Cul-1; Cullin homolog 1, cul-1
    4 View Details [527..602] PSI-BLAST 2110.0 Anaphase promoting complex (APC); Cullin homolog 1, Cul-1; Cullin homolog 1, cul-1
    5 View Details [603..742] PSI-BLAST 2110.0 Anaphase promoting complex (APC); Cullin homolog 1, Cul-1; Cullin homolog 1, cul-1
    6 View Details [743..815] PSI-BLAST 2110.0 Anaphase promoting complex (APC); Cullin homolog 1, Cul-1; Cullin homolog 1, cul-1

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • ubiquitin-protein ligase activity
    		•  9.95820721075934 bayes_pls_golite062009
  • small conjugating protein ligase activity
    		•  9.63704265478699 bayes_pls_golite062009
  • acid-amino acid ligase activity
    		•  8.59660059921406 bayes_pls_golite062009
  • ligase activity, forming carbon-nitrogen bonds
    		•  7.32718329074249 bayes_pls_golite062009
  • vasopressin activated calcium mobilizing receptor activity
    		•  6.69190468636718 bayes_pls_golite062009
  • transcription regulator activity
    		•  4.76121492612224 bayes_pls_golite062009
  • ligase activity
    		•  4.08678005991666 bayes_pls_golite062009
  • transcription factor activity
    		•  3.7855017556511 bayes_pls_golite062009
  • binding
    		•  2.45185337633142 bayes_pls_golite062009
  • transcription repressor activity
    		•  2.39237599101586 bayes_pls_golite062009
  • protein binding
    		•  2.34145982999922 bayes_pls_golite062009
  • transcription activator activity
    		•  2.05043065144612 bayes_pls_golite062009
  • nucleic acid binding
    		•  1.54797684894025 bayes_pls_golite062009
  • DNA binding
    		•  1.42215436462553 bayes_pls_golite062009
  • RNA polymerase II transcription factor activity
    		•  1.23862224095769 bayes_pls_golite062009
  • transporter activity
    		•  0.891050405674973 bayes_pls_golite062009
  • transmembrane transporter activity
    		•  0.648150681919233 bayes_pls_golite062009
  • transcription factor binding
    		•  0.612315945412079 bayes_pls_golite062009
  • substrate-specific transporter activity
    		•  0.368917395770011 bayes_pls_golite062009
  • catalytic activity
    		•  0.351553138703956 bayes_pls_golite062009
  • transferase activity, transferring phosphorus-containing groups
    		•  0.249771970501337 bayes_pls_golite062009
  • general RNA polymerase II transcription factor activity
    		•  0.146182977030285 bayes_pls_golite062009
  • hydrolase activity
    		•  0.140191206949582 bayes_pls_golite062009
  • structure-specific DNA binding
    		•  0.117670813298683 bayes_pls_golite062009
  • substrate-specific transmembrane transporter activity
    		•  0.107671044044222 bayes_pls_golite062009
  • transferase activity
    		•  0.064832527337659 bayes_pls_golite062009
  • specific transcriptional repressor activity
    		•  0.0565989703351002 bayes_pls_golite062009
  • sequence-specific DNA binding
    		•  0.0102780098866235 bayes_pls_golite062009
    2 No functions predicted.
    3 No functions predicted.
    4 No functions predicted.
    5 No functions predicted.
    6 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.98

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle