Protein Overview: LST4

General Information:
Name(s) found:	LST4 / YKL176C [SGD]
Description(s) found: Found 29 descriptions. SHOW ALL	Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface [SGD]
Organism:	Saccharomyces cerevisiae
Length:	828 amino acids
Gene Ontology:
Cellular Component:	vesicle coat [IDA]
Biological Process:	vesicle-mediated transport [IDA] protein transport [IDA] Golgi to plasma membrane transport [IDA] intracellular protein transport [IDA]
Molecular Function:	protein transporter activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLGNLLRNKT SSSGFEKSSE HSDFSSVVPN VPVYCKAAST GTTKTAAGAL LDTAVNVEKP  60
   61 SEMLSTTSPP ILDHISDDLK LKLFGSRDIP YSRPIDTLQN NGGLGTDKIT SINEKTYAFR 120
  121 ILIIEEAGQM ACRNNYRDIF DYTTSKISNS MEQIRPSELK EYIFGSPVRS SDLTQCDKIR 180
  181 TIPNSDLVLI TRIFYYTHQY NRIAISLCIP RILLPVVAES WSSISSWLTQ TQKMLIGFLT 240
  241 KNRIMQENTG NYSNNSVIKL SNIDIRTHYP KEIEIMVQTL QKRVIPGLRS MSEIPRLFLY 300
  301 PETFKEFVHV WFKSIFNWIE IKDGPKLGFL PLLMAMIISD YRHTIRELKT SKIVILSGNM 360
  361 VVANKLLFIL SALLEPKYKG QITIRRENIR SDSSAVSRNK SNNNFVDKPE TELSTLTSTD 420
  421 NLLSRTENNS NHNYNNSNVS SNSIGSPNFH SLRKGWQIPN RRNSNTSVSV SSSESLAEVI 480
  481 QPSSFKSGSS SLHYLSSSIS SQPGSYGSWF NKRPTISQFF QPSPSLKHNE SWERLQTTAG 540
  541 NMQRTSSSSS LQQATSRLSL TTPQQSPSIS EYDEYPWMGT PGSPNVGDVS HAPPLVKNIS 600
  601 YKFPLKNVEL KRDCQRISQD DLLDEAFERI CQPSLADLNS TYEIFPGNSS YADILTTDSD 660
  661 IDDGLMNKPL ELLPKYTMYL THFNNFFQLQ ACPAGQESES RITNSMKIDL LKADYTRSLL 720
  721 VSLRSRDIRD VALKREFTGN NNNNSNQNIY DENFVGKRKY VLKQKTRKIF SCGKIGKLST 780
  781 SLENCVNFVE NSIKSAMMLY DDNGIDSELR DSEALRIFSS LVHYCNAG

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Ho Y, et al. (2002)		BMS1 CDC53 CIC1 COF1 CTF4 DBP10 DED81 DIA2 ENP1 FPR3 ILS1 KRE33 KRI1 LST4 MCM2 MCM3 MCM5 NIP7 NMD3 NOP12 RRP12 SEH1 SKP1 SLT2 SPB1 SSF1 SSF2 SSZ1 TIF6 URB1 UTP10 YAK1 YBL104C

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	BOI1	Sample boi1 with gst from october 2005	McCusker D, et al (2007)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RPS28A	LST4	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	LST4	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..322]	MSA	1.002	View MSA. No confident structure predictions are available.
2	View Details	[323..513]	deduced	N/A	No confident structure predictions are available.
3	View Details	[514..828]	ab initio	7.03	No description for 1f1kA was found.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.92

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2