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Protein Overview: MCM3

General Information:
Name(s) found: MCM3 / YEL032W [SGD]
Description(s) found:
Found 23 descriptions.
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  • Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 971 amino acids

    Gene Ontology:
    Cellular Component: nucleus [IDALink to PubMed article]
    pre-replicative complex [IDALink to PubMed article]
    cytoplasm [IDALink to PubMed articleLink to PubMed article][TASLink to PubMed article]
    MCM complex [IDALink to PubMed article]
    Biological Process: DNA replication initiation [TASLink to PubMed article]
    pre-replicative complex assembly [IPILink to PubMed article]
    DNA unwinding involved in replication [TASLink to PubMed article]
    Molecular Function: chromatin binding [TASLink to PubMed article]
    ATP-dependent DNA helicase activity [TASLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MEGSTGFDGD ATTFFAPDAV FGDRVRRFQE FLDTFTSYRD SVRSIQVYNS NNAANYNDDQ  60
   61 DDADERDLLG DDDGDDLEKE KKAASSTSLN ILPHRIIISL DDLREFDRSF WSGILVEPAY 120
  121 FIPPAEKALT DLADSMDDVP HPNASAVSSR HPWKLSFKGS FGAHALSPRT LTAQHLNKLV 180
  181 SVEGIVTKTS LVRPKLIRSV HYAAKTGRFH YRDYTDATTT LTTRIPTPAI YPTEDTEGNK 240
  241 LTTEYGYSTF IDHQRITVQE MPEMAPAGQL PRSIDVILDD DLVDKTKPGD RVNVVGVFKS 300
  301 LGAGGMNQSN SNTLIGFKTL ILGNTVYPLH ARSTGVAARQ MLTDFDIRNI NKLSKKKDIF 360
  361 DILSQSLAPS IYGHDHIKKA ILLMLMGGVE KNLENGSHLR GDINILMVGD PSTAKSQLLR 420
  421 FVLNTASLAI ATTGRGSSGV GLTAAVTTDR ETGERRLEAG AMVLADRGVV CIDEFDKMTD 480
  481 VDRVAIHEVM EQQTVTIAKA GIHTTLNARC SVIAAANPVF GQYDVNRDPH QNIALPDSLL 540
  541 SRFDLLFVVT DDINEIRDRS ISEHVLRTHR YLPPGYLEGE PVRERLNLSL AVGEDADINP 600
  601 EEHSNSGAGV ENEGEDDEDH VFEKFNPLLQ AGAKLAKNKG NYNGTEIPKL VTIPFLRKYV 660
  661 QYAKERVIPQ LTQEAINVIV KNYTDLRNDD NTKKSPITAR TLETLIRLAT AHAKVRLSKT 720
  721 VNKVDAKVAA NLLRFALLGE DIGNDIDEEE SEYEEALSKR SPQKSPKKRQ RVRQPASNSG 780
  781 SPIKSTPRRS TASSVNATPS SARRILRFQD DEQNAGEDDN DIMSPLPADE EAELQRRLQL 840
  841 GLRVSPRRRE HLHAPEEGSS GPLTEVGTPR LPNVSSAGQD DEQQQSVISF DNVEPGTIST 900
  901 GRLSLISGII ARLMQTEIFE EESYPVASLF ERINEELPEE EKFSAQEYLA GLKIMSDRNN 960
  961 LMVADDKVWR V

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details (MIPS) Mewes HW, et al. (2004) CDC45 CDC6 DPB11 DPB2 DPB3 MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 POL2 POL3 POL31 POL32 SLD3
    View Details Ho Y, et al. (2002) BMS1 CDC53 CIC1 COF1 CTF4 DBP10 DED81 DIA2 ENP1 FPR3 ILS1 KRE33 KRI1 LST4 MCM2 MCM3 MCM5 NIP7 NMD3 NOP12 RRP12 SEH1 SKP1 SLT2 SPB1 SSF1 SSF2 SSZ1 TIF6 URB1 UTP10 YAK1 YBL104C
    View Details Qiu et al. (2008) CDC45 CDC6 DPB11 DPB2 DPB3 DPB4 HCS1 MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 PCC1 POB3 POL1 POL2 POL3 POL31 POL32 RFC1 RFC4 SLD3

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run MLP2 #31 Asynchronous Prep (Protease cleavage) Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

    No microscopy data found in the PDR for this protein.

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..158] MSA 1.098987 View MSA. No confident structure predictions are available.
    2 View Details [159..366] Pfam 9.086186 MCM2/3/5 family No confident structure predictions are available.
    3 View Details [367..595] PSI-BLAST 40.69897 ATPase subunit of magnesium chelatase, BchI
    4 View Details [596..669] PSI-BLAST 40.69897 ATPase subunit of magnesium chelatase, BchI
    5 View Details [670..779] ORFEUS 7.44 ATPase subunit of magnesium chelatase, BchI
    6 View Details [780..971] deduced N/A No confident structure predictions are available.

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • nucleic acid binding
    		•  4.29626483284963 bayes_pls_golite062009
  • DNA binding
    		•  3.71771169141041 bayes_pls_golite062009
  • structural constituent of ribosome
    		•  3.51998515668903 bayes_pls_golite062009
  • structural molecule activity
    		•  3.29857127235306 bayes_pls_golite062009
  • structure-specific DNA binding
    		•  3.06066809614422 bayes_pls_golite062009
  • single-stranded DNA binding
    		•  3.0183551365402 bayes_pls_golite062009
  • binding
    		•  2.83465364476231 bayes_pls_golite062009
  • transcription regulator activity
    		•  2.10110937001387 bayes_pls_golite062009
  • ligase activity, forming aminoacyl-tRNA and related compounds
    		•  2.04350689362549 bayes_pls_golite062009
  • ligase activity, forming carbon-oxygen bonds
    		•  2.04350689362549 bayes_pls_golite062009
  • aminoacyl-tRNA ligase activity
    		•  2.04218282227834 bayes_pls_golite062009
  • DNA replication origin binding
    		•  1.84819558112187 bayes_pls_golite062009
  • telomeric DNA binding
    		•  1.7847835613194 bayes_pls_golite062009
  • single-stranded telomeric DNA binding
    		•  1.48033977789232 bayes_pls_golite062009
  • translation regulator activity
    		•  1.46320772716954 bayes_pls_golite062009
  • translation factor activity, nucleic acid binding
    		•  1.40718318783582 bayes_pls_golite062009
  • transcription repressor activity
    		•  1.30343463892105 bayes_pls_golite062009
  • translation initiation factor activity
    		•  1.27539679024898 bayes_pls_golite062009
  • telomerase inhibitor activity
    		•  1.25988289474883 bayes_pls_golite062009
  • DNA-directed RNA polymerase activity
    		•  1.19766815287926 bayes_pls_golite062009
  • RNA polymerase activity
    		•  1.19766815287926 bayes_pls_golite062009
  • ribonuclease activity
    		•  1.19236946227657 bayes_pls_golite062009
  • transcription factor activity
    		•  1.14242112812892 bayes_pls_golite062009
  • DNA helicase activity
    		•  1.12203697857261 bayes_pls_golite062009
  • ligase activity
    		•  1.06979791212194 bayes_pls_golite062009
  • sequence-specific DNA binding
    		•  0.85691075793641 bayes_pls_golite062009
  • DNA-dependent ATPase activity
    		•  0.638831894247026 bayes_pls_golite062009
  • nuclease activity
    		•  0.57381531077345 bayes_pls_golite062009
  • aspartate-tRNA ligase activity
    		•  0.504302440847948 bayes_pls_golite062009
  • nucleotidyltransferase activity
    		•  0.325774164899685 bayes_pls_golite062009
  • exonuclease activity
    		•  0.28313884527829 bayes_pls_golite062009
  • RNA binding
    		•  0.18502033889261 bayes_pls_golite062009
  • protein binding
    		•  0.152645195799531 bayes_pls_golite062009
  • exoribonuclease activity, producing 5'-phosphomonoesters
    		•  0.125256208482441 bayes_pls_golite062009
  • exoribonuclease activity
    		•  0.109774918833391 bayes_pls_golite062009
  • exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters
    		•  0.0954216620147701 bayes_pls_golite062009
  • ATP-dependent DNA helicase activity
    		•  0.0179535526984695 bayes_pls_golite062009
  • helicase activity
    		•  0.0121541775159524 bayes_pls_golite062009
    2 No functions predicted.
    3 No functions predicted.
    4 No functions predicted.
    5 No functions predicted.
    6 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle