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Protein Overview: RFC1

General Information:
Name(s) found: RFC1 / YOR217W [SGD]
Description(s) found:
Found 30 descriptions.
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  • Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 861 amino acids

    Gene Ontology:
    Cellular Component: DNA replication factor C complex [IDALink to PubMed article]
    Biological Process: DNA repair [IMPLink to PubMed article]
    leading strand elongation [IDALink to PubMed article]
    mismatch repair [TASLink to PubMed article]
    Molecular Function: DNA clamp loader activity [IDALink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MVNISDFFGK NKKSVRSSTS RPTRQVGSSK PEVIDLDTES DQESTNKTPK KMPVSNVIDV  60
   61 SETPEGEKKL PLPAKRKASS PTVKPASSKK TKPSSKSSDS ASNITAQDVL DKIPSLDLSN 120
  121 VHVKENAKFD FKSANSNADP DEIVSEIGSF PEGKPNCLLG LTIVFTGVLP TLERGASEAL 180
  181 AKRYGARVTK SISSKTSVVV LGDEAGPKKL EKIKQLKIKA IDEEGFKQLI AGMPAEGGDG 240
  241 EAAEKARRKL EEQHNIATKE AELLVKKEEE RSKKLAATRV SGGHLERDNV VREEDKLWTV 300
  301 KYAPTNLQQV CGNKGSVMKL KNWLANWENS KKNSFKHAGK DGSGVFRAAM LYGPPGIGKT 360
  361 TAAHLVAQEL GYDILEQNAS DVRSKTLLNA GVKNALDNMS VVGYFKHNEE AQNLNGKHFV 420
  421 IIMDEVDGMS GGDRGGVGQL AQFCRKTSTP LILICNERNL PKMRPFDRVC LDIQFRRPDA 480
  481 NSIKSRLMTI AIREKFKLDP NVIDRLIQTT RGDIRQVINL LSTISTTTKT INHENINEIS 540
  541 KAWEKNIALK PFDIAHKMLD GQIYSDIGSR NFTLNDKIAL YFDDFDFTPL MIQENYLSTR 600
  601 PSVLKPGQSH LEAVAEAANC ISLGDIVEKK IRSSEQLWSL LPLHAVLSSV YPASKVAGHM 660
  661 AGRINFTAWL GQNSKSAKYY RLLQEIHYHT RLGTSTDKIG LRLDYLPTFR KRLLDPFLKQ 720
  721 GADAISSVIE VMDDYYLTKE DWDSIMEFFV GPDVTTAIIK KIPATVKSGF TRKYNSMTHP 780
  781 VAIYRTGSTI GGGGVGTSTS TPDFEDVVDA DDNPVPADDE ETQDSSTDLK KDKLIKQKAK 840
  841 PTKRKTATSK PGGSKKRKTK A

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) RFC1 RFC2 RFC3 RFC4 RFC5
    View Details (MIPS) Mewes HW, et al. (2004) RFC1 RFC2 RFC3 RFC4 RFC5
    View Details Krogan NJ, et al. (2006) ELG1 RFC1 RFC2 RFC3 RFC4 RFC5
    View Details Gavin AC, et al. (2006) ELG1 RFC1 RFC2 RFC3 RFC4 RFC5
    View Details Gavin AC, et al. (2002) CTF18 CTF8 DPB2 ELG1 MIS1 OLA1 POL1 POL12 POL2 PRI1 PRI2 RFC1 RFC2 RFC3 RFC4 RFC5 TOP2 USO1 VPS13 YMR181C
    View Details Ho Y, et al. (2002) ACO1 ADE5,7 ADH2 AHA1 ELG1 GUS1 HSP104 RFC1 RFC2 RFC4 RNQ1 RPT3 SAN1 YHR020W
    View Details Qiu et al. (2008) CDC45 DPB11 DPB2 DPB4 MCM2 MCM3 MCM4 MCM6 MCM7 POL3 POL31 POL32 RFC1 RFC2 RFC3 RFC4 RFC5 RRM3

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run MLP2 #25 Asynchronous Prep3-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #32 Asynchronous Prep-No Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #31 Asynchronous Prep (Protease cleavage) Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data RFC1 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..196] PSI-BLAST 70.0 Membrane fusion atpase p97 N-terminal domain , P97-Nn; Membrane fusion atpase p97, D1 domain; Membrane fusion atpase p97 domain 2, P97-Nc
    2 View Details [197..280] PSI-BLAST 70.0 Membrane fusion atpase p97 N-terminal domain , P97-Nn; Membrane fusion atpase p97, D1 domain; Membrane fusion atpase p97 domain 2, P97-Nc
    3 View Details [281..301]
    [479..542]    		 PSI-BLAST 207.06601 Replication factor C
    4 View Details [302..478]
    [543..663]    		 PSI-BLAST 207.06601 Replication factor C
    5 View Details [664..861] deduced N/A No confident structure predictions are available.

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2
    Term Confidence Notes
  • DNA clamp loader activity
    		•  6.51495346697904 bayes_pls_golite062009
  • protein-DNA loading ATPase activity
    		•  6.40748863504898 bayes_pls_golite062009
  • DNA-dependent ATPase activity
    		•  2.72462789867829 bayes_pls_golite062009
  • DNA helicase activity
    		•  2.2817164193758 bayes_pls_golite062009
  • pyrophosphatase activity
    		•  2.13284212711072 bayes_pls_golite062009
  • ATP-dependent DNA helicase activity
    		•  1.88455339138556 bayes_pls_golite062009
  • structure-specific DNA binding
    		•  1.88004981070099 bayes_pls_golite062009
  • mismatched DNA binding
    		•  1.75879616923546 bayes_pls_golite062009
  • binding
    		•  1.62320027057768 bayes_pls_golite062009
  • microtubule motor activity
    		•  1.30090199952739 bayes_pls_golite062009
    		• 1.27220313435557 bayes_pls_golite062009
  • guanine/thymine mispair binding
    		•  1.10234539735776 bayes_pls_golite062009
  • motor activity
    		•  1.03253378953548 bayes_pls_golite062009
  • helicase activity
    		•  1.0321619822241 bayes_pls_golite062009
  • catalytic activity
    		•  1.00104173493322 bayes_pls_golite062009
  • DNA-directed DNA polymerase activity
    		•  0.971476020912368 bayes_pls_golite062009
  • DNA insertion or deletion binding
    		•  0.94261959567056 bayes_pls_golite062009
  • DNA polymerase activity
    		•  0.928091843881235 bayes_pls_golite062009
  • nucleic acid binding
    		•  0.833704518618903 bayes_pls_golite062009
  • ATP-dependent helicase activity
    		•  0.5607032154839 bayes_pls_golite062009
  • purine NTP-dependent helicase activity
    		•  0.5607032154839 bayes_pls_golite062009
  • double-stranded DNA binding
    		•  0.35687254758087 bayes_pls_golite062009
  • DNA binding
    		•  0.277415156373835 bayes_pls_golite062009
  • nucleoside-triphosphatase activity
    		•  0.147579541298736 bayes_pls_golite062009
  • nucleotide binding
    		•  0.114848452385726 bayes_pls_golite062009
  • hydrolase activity, acting on acid anhydrides
    		•  0.113504049290503 bayes_pls_golite062009
  • hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
    		•  0.111293884322157 bayes_pls_golite062009
  • purine nucleotide binding
    		•  0.10713474095071 bayes_pls_golite062009
  • purine ribonucleotide binding
    		•  0.0916519414118148 bayes_pls_golite062009
  • ribonucleotide binding
    		•  0.0915913646217636 bayes_pls_golite062009
  • structural constituent of ribosome
    		•  0.089234257580272 bayes_pls_golite062009
  • transcription regulator activity
    		•  0.0109066016297058 bayes_pls_golite062009
    3 No functions predicted.
    4 No functions predicted.
    5 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.98

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle