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Protein Overview: TOP2

General Information:
Name(s) found: TOP2 / YNL088W [SGD]
Description(s) found:
Found 27 descriptions.
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  • Essential type II topoisomerase, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 1428 amino acids

    Gene Ontology:
    Cellular Component: nucleus [IDALink to PubMed article]
    mitochondrion [IDALink to PubMed article]
    synaptonemal complex [IDALink to PubMed article]
    Biological Process: reciprocal meiotic recombination [IMPLink to PubMed article]
    DNA strand elongation involved in DNA replication [IMPLink to PubMed article]
    regulation of mitotic recombination [IMPLink to PubMed article]
    chromatin assembly or disassembly [IMPLink to PubMed article]
    DNA topological change [IDALink to PubMed article]
    Molecular Function: DNA topoisomerase (ATP-hydrolyzing) activity [IDALink to PubMed articleLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSTEPVSASD KYQKISQLEH ILKRPDTYIG SVETQEQLQW IYDEETDCMI EKNVTIVPGL  60
   61 FKIFDEILVN AADNKVRDPS MKRIDVNIHA EEHTIEVKND GKGIPIEIHN KENIYIPEMI 120
  121 FGHLLTSSNY DDDEKKVTGG RNGYGAKLCN IFSTEFILET ADLNVGQKYV QKWENNMSIC 180
  181 HPPKITSYKK GPSYTKVTFK PDLTRFGMKE LDNDILGVMR RRVYDINGSV RDINVYLNGK 240
  241 SLKIRNFKNY VELYLKSLEK KRQLDNGEDG AAKSDIPTIL YERINNRWEV AFAVSDISFQ 300
  301 QISFVNSIAT TMGGTHVNYI TDQIVKKISE ILKKKKKKSV KSFQIKNNMF IFINCLIENP 360
  361 AFTSQTKEQL TTRVKDFGSR CEIPLEYINK IMKTDLATRM FEIADANEEN ALKKSDGTRK 420
  421 SRITNYPKLE DANKAGTKEG YKCTLVLTEG DSALSLAVAG LAVVGRDYYG CYPLRGKMLN 480
  481 VREASADQIL KNAEIQAIKK IMGLQHRKKY EDTKSLRYGH LMIMTDQDHD GSHIKGLIIN 540
  541 FLESSFPGLL DIQGFLLEFI TPIIKVSITK PTKNTIAFYN MPDYEKWREE ESHKFTWKQK 600
  601 YYKGLGTSLA QEVREYFSNL DRHLKIFHSL QGNDKDYIDL AFSKKKADDR KEWLRQYEPG 660
  661 TVLDPTLKEI PISDFINKEL ILFSLADNIR SIPNVLDGFK PGQRKVLYGC FKKNLKSELK 720
  721 VAQLAPYVSE CTAYHHGEQS LAQTIIGLAQ NFVGSNNIYL LLPNGAFGTR ATGGKDAAAA 780
  781 RYIYTELNKL TRKIFHPADD PLYKYIQEDE KTVEPEWYLP ILPMILVNGA EGIGTGWSTY 840
  841 IPPFNPLEII KNIRHLMNDE ELEQMHPWFR GWTGTIEEIE PLRYRMYGRI EQIGDNVLEI 900
  901 TELPARTWTS TIKEYLLLGL SGNDKIKPWI KDMEEQHDDN IKFIITLSPE EMAKTRKIGF 960
  961 YERFKLISPI SLMNMVAFDP HGKIKKYNSV NEILSEFYYV RLEYYQKRKD HMSERLQWEV1020
 1021 EKYSFQVKFI KMIIEKELTV TNKPRNAIIQ ELENLGFPRF NKEGKPYYGS PNDEIAEQIN1080
 1081 DVKGATSDEE DEESSHEDTE NVINGPEELY GTYEYLLGMR IWSLTKERYQ KLLKQKQEKE1140
 1141 TELENLLKLS AKDIWNTDLK AFEVGYQEFL QRDAEARGGN VPNKGSKTKG KGKRKLVDDE1200
 1201 DYDPSKKNKK STARKGKKIK LEDKNFERIL LEQKLVTKSK APTKIKKEKT PSVSETKTEE1260
 1261 EENAPSSTSS SSIFDIKKED KDEGELSKIS NKFKKISTIF DKMGSTSATS KENTPEQDDV1320
 1321 ATKKNQTTAK KTAVKPKLAK KPVRKQQKVV ELSGESDLEI LDSYTDREDS NKDEDDAIPQ1380
 1381 RSRRQRSSRA ASVPKKSYVE TLELSDDSFI EDDEEENQGS DVSFNEED

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) FUN12 POB3 RPL15A RPL4B RPP0 RPS11A, RPS11B RPS1A RPS1B RPS3 RPS8B, RPS8A TOP2 UTP22 YLR455W
    View Details (MIPS) Mewes HW, et al. (2004) HOP1 RED1 TOP1 TOP2 ZIP1 ZIP2
    View Details Krogan NJ, et al. (2006) ABF2 ARG82 CKI1 CMR1 HHF1, HHF2 HTA1 HTA2 HTB1 HTB2 NUP188 PPH3 PSY2 PSY4 RRD1 SLX5 SLX8 SPT5 STP1 TOP2
    View Details Gavin AC, et al. (2002) AVO1 BEM2 CEG1 CET1 CLU1 CTF18 CTF8 CTR9 DPB2 ELG1 HHF1, HHF2 LSM12 NIP1 NOP1 OLA1 PDB1 POL2 PRP43 RFC1 RFC2 RFC3 RFC4 RFC5 RPG1 SPT16 SSA3 SSA4 TOP2 VPS1 VPS13 YMR181C
    View Details Ho Y, et al. (2002) CDC10 CDC11 CDC12 CDC3 CKA2 CKB1 CLU1 DUN1 LSP1 NOP56 PIL1 RNH203 RPN1 TOP2 YRA1 YTA6
    View Details Qiu et al. (2008) HOP1 RED1 TOP2

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run CEP3 sample cbf3 from feb 2005 Sandall S, et all (2006)
    View Run RLF2 phosphorylation data - cascade search Green EM, et al (2005)
    View Run RLF2 identification data - first search in cascade Green EM, et al (2005)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data TOP2 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..248] PSI-BLAST 302.218487 DNA gyrase B
    2 View Details [249..405] PSI-BLAST 302.218487 DNA gyrase B
    3 View Details [406..561]
    [604..656]    		 PSI-BLAST 11000.0 DNA topoisomerase II, C-terminal fragment (residues 410-1202)
    4 View Details [562..603]
    [657..701]
    [792..803]
    [816..879]
    [970..1012]
    [1147..1165]    		 PSI-BLAST 11000.0 DNA topoisomerase II, C-terminal fragment (residues 410-1202)
    5 View Details [702..791]
    [804..815]
    [880..969]
    [1013..1146]
    [1166..1207]    		 PSI-BLAST 11000.0 DNA topoisomerase II, C-terminal fragment (residues 410-1202)
    6 View Details [1208..1428] MSA 2.140981 View MSA. No confident structure predictions are available.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle