Protein Overview: SLX8

General Information:
Name(s) found:	SLX8 / YER116C [SGD]
Description(s) found: Found 26 descriptions. SHOW ALL	Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain [SGD]
Organism:	Saccharomyces cerevisiae
Length:	274 amino acids
Gene Ontology:
Cellular Component:	cellular_component [ND]
Biological Process:	protein sumoylation [IGI][IMP] telomere maintenance [IMP ] response to DNA damage stimulus [IGI ][IMP ]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MARRPDNQNP EGENLRIKRV RLESVRQNDE EEENEVSRTQ NIVTDNRHDS PEAVVEIIGE  60
   61 RALENTSEED GDDDLSLFRA LEEDPGSDHN TSNNDSGNHD RETMHTEEPE ASSGNNITLT 120
  121 NNVEELHTMD VLSQTANTPS ASPMLDAAPP TTKPGTNSKE QTVDLTADAI DLDAEEQQVL 180
  181 QISDDDFQEE TKEAPKEYGA AKDYRCPICF EPPETALMTL CGHVFCCPCL FQMVNSSRTC 240
  241 RQFGHCALCR SKVYLKDVRL IILRKKQVKK KVKS

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		ABF2 ARG82 CKI1 CMR1 HHF1, HHF2 HTA1 HTA2 HTB1 HTB2 NUP188 PPH3 PSY2 PSY4 RRD1 SLX5 SLX8 SPT5 STP1 TOP2
View Details	Qiu et al. (2008)		AST2 PUF2 SLX8

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	ASF1	sample 3928 - Asf1-S-TEV-ZZ (in hir3delta mutant)	Green EM, et al (2005)
View Run	MLP2	#14 Mitotic Prep2-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	THG1	SLX8	2	Hazbun TR, et al. (2003)
View Screen	THG1	SLX8	2	Hazbun TR, et al. (2003)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	SLX8	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..198]	MSA	1.974995	View MSA. No confident structure predictions are available.
2	View Details	[199..274]	PSI-BLAST	42.30103	brca1 RING domain

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
ubiquitin-protein ligase activity	10.2497392096259	bayes_pls_golite062009
small conjugating protein ligase activity	10.0460587929138	bayes_pls_golite062009
acid-amino acid ligase activity	8.99384576623969	bayes_pls_golite062009
ligase activity, forming carbon-nitrogen bonds	7.6988425139962	bayes_pls_golite062009
ligase activity	3.92677358871643	bayes_pls_golite062009
binding	2.58862749918018	bayes_pls_golite062009
protein binding	1.49775478023418	bayes_pls_golite062009
small conjugating protein-specific protease activity	1.2126425713931	bayes_pls_golite062009
ubiquitin-specific protease activity	1.03469202541773	bayes_pls_golite062009
transcription regulator activity	0.919313413645213	bayes_pls_golite062009
nucleic acid binding	0.894017701424745	bayes_pls_golite062009
DNA binding	0.588609879350932	bayes_pls_golite062009
cysteine-type peptidase activity	0.50197530717959	bayes_pls_golite062009
ubiquitin thiolesterase activity	0.265769062717221	bayes_pls_golite062009
transcription factor binding	0.14168789797081	bayes_pls_golite062009
catalytic activity	0.14112008370434	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.96

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2