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Protein Overview: PUF2

General Information:
Name(s) found: PUF2 / YPR042C [SGD]
Description(s) found:
Found 26 descriptions.
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  • Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 1075 amino acids

    Gene Ontology:
    Cellular Component: cytoplasm [IDALink to PubMed article]
    Biological Process: nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [IGILink to PubMed article]
    Molecular Function: mRNA binding [IDALink to PubMed article][ISSLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDNKRLYNGN LSNIPEVIDP GITIPIYEED IRNDTRMNTN ARSVRVSDKR GRSSSTSPQK  60
   61 IGSYRTRAGR FSDTLTNLLP SISAKLHHSK KSTPVVVVPP TSSTPDSLNS TTYAPRVSSD 120
  121 SFTVATPLSL QSTTTRTRTR NNTVSSQITA SSSLTTDVGN ATSANIWSAN AESNTSSSPL 180
  181 FDYPLATSYF EPLTRFKSTD NYTLPQTAQL NSFLEKNGNP NIWSSAGNSN TDHLNTPIVN 240
  241 RQRSQSQSTT NRVYTDAPYY QQPAQNYQVQ VPPRVPKSTS ISPVILDDVD PASINWITAN 300
  301 QKVPLVNQIS ALLPTNTISI SNVFPLQPTQ QHQQNAVNLT STSLATLCSQ YGKVLSARTL 360
  361 RGLNMALVEF STVESAICAL EALQGKELSK VGAPSTVSFA RVLPMYEQPL NVNGFNNTPK 420
  421 QPLLQEQLNH GVLNYQLQQS LQQPELQQQP TSFNQPNLTY CNPTQNLSHL QLSSNENEPY 480
  481 PFPLPPPSLS DSKKDILHTI SSFKLEYDHL ELNHLLQNAL KNKGVSDTNY FGPLPEHNSK 540
  541 VPKRKDTFDA PKLRELRKQF DSNSLSTIEM EQLAIVMLDQ LPELSSDYLG NTVIQKLFEN 600
  601 SSNIIRDIML RKCNKYLTSM GVHKNGTWVC QKIIKMANTP RQINLVTSGV SDYCTPLFND 660
  661 QFGNYVIQGI LKFGFPWNSF IFESVLSHFW TIVQNRYGSR AVRACLEADS IITQCQLLTI 720
  721 TSLIIVLSPY LATDTNGTLL ITWLLDTCTL PNKNLILCDK LVNKNLVKLC CHKLGSLTVL 780
  781 KILNLRGGEE EALSKNKIIH AIFDGPISSD SILFQILDEG NYGPTFIYKV LTSRILDNSV 840
  841 RDEAITKIRQ LILNSNINLQ SRQLLEEVGL SSAGISPKQS SKNHRKQHPQ GFHSPGRARG 900
  901 VSVSSVRSSN SRHNSVIQMN NAGPTPALNF NPAPMSEINS YFNNQQVVYS GNQNQNQNGN 960
  961 SNGLDELNSQ FDSFRIANGT NLSLPIVNLP NVSNNNNNYN NSGYSSQMNP LSRSVSHNNN1020
 1021 NNTNNYNNND NDNNNNNNNN NNNNNNNNNN NNNSNNSNNN NNNDTSLYRY RSYGY

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Qiu et al. (2008) AST2 FRE8 HMS2 MLP2 MNL1 NAT3 PUF2 RPL12B, RPL12A RPL17A RPL20B RPL21A RPL26A RPL27B RPL31B RPL34A RPL35A, RPL35B RPL37A RPL40B, RPL40A RPL41A, RPL41B RPL42B, RPL42A RPP2A RPS0A RPS0B RPS16B, RPS16A RPS17A RPS18B, RPS18A RPS19B RPS23B, RPS23A RPS24A, RPS24B RPS25B RPS26A RPS6A, RPS6B RPS8B, RPS8A RXT3 SFT1 SLX8 UGO1 UTP6 YDL133W YGK3 YGR117C YGR149W YLR030W YOR292C

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run CEP3 sample cbf3 from feb 2005 Sandall S, et all (2006)
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run VAM3 Sample: HX13 - trans-SNARE complex forms but fusion is blocked. Hao Xu, et al. (2010)
    View Run MLP2 #24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #29 Asynchronous Prep5-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run None Entered #17 Mitotic Prep3-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data PUF2 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..279] MSA 15.438997 View MSA. No confident structure predictions are available.
    2 View Details [280..454] Pfam 3.040959 RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain) No confident structure predictions are available.
    3 View Details [455..511] deduced N/A No confident structure predictions are available.
    4 View Details [512..650] PSI-BLAST 200.0103 Pumilio 1
    5 View Details [651..751] PSI-BLAST 200.0103 Pumilio 1
    6 View Details [752..899] PSI-BLAST 200.0103 Pumilio 1
    7 View Details [900..1075] deduced N/A No confident structure predictions are available.

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2 No functions predicted.
    3 No functions predicted.
    4
    Term Confidence Notes
  • RNA binding
    		•  4.2310970544603 bayes_pls_golite062009
  • mRNA binding
    		•  3.1237691117539 bayes_pls_golite062009
  • nucleic acid binding
    		•  2.87272982027282 bayes_pls_golite062009
  • binding
    		•  2.24760917108348 bayes_pls_golite062009
    		• 1.12277203340218 bayes_pls_golite062009
  • single-stranded RNA binding
    		•  1.08300488237934 bayes_pls_golite062009
  • protein binding
    		•  0.361619805766015 bayes_pls_golite062009
  • hydrolase activity
    		•  0.181856784298799 bayes_pls_golite062009
  • poly-pyrimidine tract binding
    		•  0.137774615811754 bayes_pls_golite062009
  • transcription regulator activity
    		•  0.0600612010525731 bayes_pls_golite062009
  • DNA binding
    		•  0.0239003246213715 bayes_pls_golite062009
  • mRNA 3'-UTR binding
    		•  0.00441912341140061 bayes_pls_golite062009
    5 No functions predicted.
    6
    Term Confidence Notes
  • RNA binding
    		•  2.11573661150436 bayes_pls_golite062009
  • binding
    		•  1.83406697369076 bayes_pls_golite062009
  • protein binding
    		•  1.04044934052933 bayes_pls_golite062009
  • nucleic acid binding
    		•  0.967597910409065 bayes_pls_golite062009
  • mRNA binding
    		•  0.73831124805124 bayes_pls_golite062009
  • hydrolase activity
    		•  0.0579816100130538 bayes_pls_golite062009
  • transcription regulator activity
    		•  0.022274123463527 bayes_pls_golite062009
    7 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.94

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle