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Run Information:

Bait Protein: ASF1
Organism: Saccharomyces cerevisiae
Publication: Green EM, Antczak AJ, Bailey AO, Franco AA, Wu KJ, Yates JR 3rd, Kaufman PD. Replication-independent histone deposition by the HIR complex and Asf1. Curr Biol. 2005 Nov 22;15(22):2044-9.
Comments: sample 3928 - Asf1-S-TEV-ZZ (in hir3delta mutant)

Raw Data:

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FILTERED Run Results: [unfilter]

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   ASF1 Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein t... 38 118 56.6% 0.0649 31603
[View Peptides]   EFB1 Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP fo... 9 11 39.3% 0.00819 22627
[View Peptides]   TEF1, TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... 20 47 37.3% 0.0157 50033
[View Peptides]   RAD53 Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autopho... 26 31 36.9% 0.00579 91962
[View Peptides]   RPP1A Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation facto... 2 5 32.1% 0.00724 10908
[View Peptides]   CDC33 Cytoplasmic mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex ... 5 5 28.6% 0.0036 24254
[View Peptides]   MLC1 Essential light chain for Myo1p, light chain for Myo2p; stabilizes Myo2p by binding to the neck regi... 2 2 27.5% 0.00206 16444
[View Peptides]   STM1 Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with r... 6 7 22.0% 0.00393 29995
[View Peptides]   DCD1 Deoxycytidine monophosphate (dCMP) deaminase required for dCTP and dTTP synthesis; expression is NOT... 2 2 20.5% 9.84E-4 35646
[View Peptides]   IBI2 Protein of unknown function that associates with ribosomes; green fluorescent protein (GFP)-fusion p... 2 2 17.8% 0.00156 22179
[View Peptides]   SRO9 Cytoplasmic RNA-binding protein that associates with translating ribosomes; involved in heme regulat... 4 4 17.1% 0.00141 48060
[View Peptides]   VMA2 Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... 5 5 16.4% 0.00148 57749
[View Peptides]   YDJ1 Protein chaperone involved in regulation of the HSP90 and HSP70 functions; involved in protein trans... 5 5 13.2% 0.00188 44671
[View Peptides]   ARC1 Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering t... 3 3 12.8% 0.00122 42084
[View Peptides]   PMA1 Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... 5 6 12.1% 0.001 99619
[View Peptides]   YHR020W Protein of unknown function that may interact with ribosomes, based on co-purification experiments; ... 5 5 11.9% 0.00112 77386
[View Peptides]   DED1 ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all ye... 3 4 10.1% 0.00102 65553
[View Peptides]   NPL3 RNA-binding protein that promotes elongation, regulates termination, and carries poly(A) mRNA from n... 2 3 9.4% 0.00111 45407
[View Peptides]   SSZ1 Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that ... 3 3 8.7% 8.56E-4 58238
[View Peptides]   AZR1 Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole dr... 2 2 8.6% 5.01E-4 67169
[View Peptides]   NSR1 Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ri... 3 3 8.2% 0.00111 44535
[View Peptides]   PMA2 Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator ... 3 4 7.5% 6.48E-4 102172
[View Peptides]   SLX8 Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachm... 2 3 7.3% 0.00168 30764
[View Peptides]   BFR2 Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multi... 2 2 7.1% 5.75E-4 61203
[View Peptides]   AVT4 Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seve... 2 2 6.6% 4.3E-4 80026
[View Peptides]   GFA1 Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glut... 2 2 6.4% 4.28E-4 80047
[View Peptides]   HIR2 Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene tra... 3 3 6.3% 5.26E-4 98445
[View Peptides]   DBP2 Essential ATP-dependent RNA helicase of the DEAD-box protein family, involved in nonsense-mediated m... 2 2 5.5% 5.62E-4 60999
[View Peptides]   YEF3 Translational elongation factor 3, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes b... 2 2 3.0% 2.94E-4 115945
[View Peptides]   YLR001C Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purifi... 2 2 1.3% 3.56E-4 97618

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