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Protein Overview: SSA3

General Information:
Name(s) found: SSA3 / YBL075C [SGD]
Description(s) found:
Found 25 descriptions.
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  • ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 649 amino acids

    Gene Ontology:
    Cellular Component: cytosol [IDALink to PubMed article]
    Biological Process: SRP-dependent cotranslational protein targeting to membrane, translocation [IMPLink to PubMed article]
    protein folding [IGILink to PubMed article]
    response to stress [IMPLink to PubMed article]
    Molecular Function: ATPase activity [IGILink to PubMed article][ISSLink to PubMed article]
    unfolded protein binding [IGILink to PubMed article][ISSLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSRAVGIDLG TTYSCVAHFS NDRVEIIAND QGNRTTPSYV AFTDTERLIG DAAKNQAAIN  60
   61 PHNTVFDAKR LIGRKFDDPE VTTDAKHFPF KVISRDGKPV VQVEYKGETK TFTPEEISSM 120
  121 VLSKMKETAE NYLGTTVNDA VVTVPAYFND SQRQATKDAG TIAGMNVLRI INEPTAAAIA 180
  181 YGLDKKGRAE HNVLIFDLGG GTFDVSLLSI DEGVFEVKAT AGDTHLGGED FDNRLVNHLA 240
  241 TEFKRKTKKD ISNNQRSLRR LRTAAERAKR ALSSSSQTSI EIDSLFEGMD FYTSLTRARF 300
  301 EELCADLFRS TLEPVEKVLK DSKLDKSQID EIVLVGGSTR IPKIQKLVSD FFNGKEPNRS 360
  361 INPDEAVAYG AAVQAAILTG DQSTKTQDLL LLDVAPLSLG IETAGGIMTK LIPRNSTIPT 420
  421 KKSETFSTYA DNQPGVLIQV FEGERTRTKD NNLLGKFELS GIPPAPRGVP QIDVTFDIDA 480
  481 NGILNVSALE KGTGKSNKIT ITNDKGRLSK DDIDRMVSEA EKYRADDERE AERVQAKNQL 540
  541 ESYAFTLKNT INEASFKEKV GEDDAKRLET ASQETIDWLD ASQAASTDEY KDRQKELEGI 600
  601 ANPIMTKFYG AGAGAGPGAG ESGGFPGSMP NSGATGGGED TGPTVEEVD

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) CMR2 POR1 SSA3 SSB2
    View Details Krogan NJ, et al. (2006) APP1 CST6 HMO1 MCM10 MIG1 SSA3 YRM1
    View Details Gavin AC, et al. (2002) ACT1 AIM31 AVO1 BEM2 CEG1 CET1 CFT1 CFT2 CKA1 CLP1 CLU1 CTR9 ENO2 FIP1 GLC7 HHF1, HHF2 HTA1 LSM12 MPE1 NIP1 NOP1 PAP1 PCF11 PDB1 PFK1 PFS2 PRP43 PTA1 PTI1 REF2 RNA14 RNA15 RPG1 RSA3 SEC13 SEC31 SPT16 SSA3 SSA4 SSU72 SYC1 TAF6 TIF4632 TOP2 TYE7 VID24 VMR1 VPS1 VPS53 YCL046W YSH1 YTH1

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run MIF2 No Comments Cheeseman IM, et al. (2002)
    View Run SLI15 No Comments Cheeseman IM, et al. (2002)
    View Run STU1 No Comments Cheeseman IM, et al. (2002)
    View Run TOR1 No Comments Reinke A, et al. (2004)
    View Run BIR1 No Comments Widlund PO, et al. (2005)
    View Run TOR1 No Comments Reinke A, et al. (2004)
    View Run TOR2 No Comments Reinke A, et al. (2004)
    View Run LST8 No Comments Reinke A, et al. (2004)
    View Run TOR2 No Comments Reinke A, et al. (2004)
    View Run KOG1 No Comments Reinke A, et al. (2004)
    View Run TOR2 No Comments Reinke A, et al. (2004)
    View Run PAN1 No Comments Toshima J, et al (2006)
    View Run CLB2 No Comments McCusker D, et al (2007)
    View Run CLB2 No Comments McCusker D, et al (2007)
    View Run CLB2 No Comments McCusker D, et al (2007)
    View Run SEC10 No Comments De Craene, J., et al. (2006)
    View Run DAD1 Dam1 complex purified from yeast with Dad1-TAP Shimogawa MM, et al. (2006)
    View Run RPA135 his-HA tag on RPA135 Schneider, DA, et al. (2006)
    View Run RPA135 his-HA tag on RPA135 Schneider, DA, et al. (2006)
    View Run RPA135 his-HA tag on RPA135 Schneider, DA, et al. (2006)
    View Run RPA135 his-HA tag on RPA135 Schneider, DA, et al. (2006)
    View Run DAD1 Dam1-765 complex purified from yeast with Dad1-TAP Shimogawa MM, et al. (2006)
    View Run MIH1 control 1 McCusker D, et al (2007)
    View Run MIH1 control 2 McCusker D, et al (2007)
    View Run MIH1 experimental1 - mih1-1 McCusker D, et al (2007)
    View Run MIH1 experimental2 - mih1-2 McCusker D, et al (2007)
    View Run ASF1 sample 3121 run on DecaLCQ: searched for phosphorylation Green EM, et al (2005)
    View Run CEP3 sample cbf3 from feb 2005 Sandall S, et all (2006)
    View Run ASF1 3906: TAP-tagged Green EM, et al (2005)
    View Run ASF1 3908: TAP-tagged, includes hir2(delta) in background Green EM, et al (2005)
    View Run ASF1 3912: TAP-tagged, strain background includes hpc2(delta) Green EM, et al (2005)
    View Run RLF2 cac1: TAP-tagged Green EM, et al (2005)
    View Run DAD1 Dam1-765 complex purified from yeast with Dad1-TAP Shimogawa MM, et al. (2006)
    View Run DAD1 Dam1 complex purified from yeast with Dad1-TAP Shimogawa MM, et al. (2006)
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run RLF2 phosphorylation data - cascade search Green EM, et al (2005)
    View Run RLF2 identification data - first search in cascade Green EM, et al (2005)
    View Run ZDS1 Sample 2- cpn2 from june 2005 McCusker D, et al (2007)
    View Run ZDS1 Sample 3 - cpn + atp, from june 2005 McCusker D, et al (2007)
    View Run ZDS1 Sample zds1 from august 2005 McCusker D, et al (2007)
    View Run BOI1 Sample boi 1 with ha tag from october 2005 McCusker D, et al (2007)
    View Run BOI1 Sample boi1 with gst from october 2005 McCusker D, et al (2007)
    View Run BOI1 Sample bob1 (2nd set) from october 2005 McCusker D, et al (2007)
    View Run BOI2 Boi 2 gst control McCusker D, et al (2007)
    View Run MLP2 #19 Asynchronous Prep1-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #03 Alpha Factor Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #14 Mitotic Prep2-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #13 Mitotic Prep2-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #19b Asynchronous Prep1-new search criteria Keck JM, et al. (2011)
    View Run MLP2 #19a Asynchronous Prep1-new search criteria Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data SSA3 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..173]
    [364..381]    		 PSI-BLAST 2750.0 Heat shock protein 70kDa, ATPase fragment
    2 View Details [174..229]
    [305..363]    		 PSI-BLAST 2750.0 Heat shock protein 70kDa, ATPase fragment
    3 View Details [230..304] PSI-BLAST 2750.0 Heat shock protein 70kDa, ATPase fragment
    4 View Details [382..554] PSI-BLAST 739.37734 DnaK
    5 View Details [555..649] ORFEUS 7.52 DnaK

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • unfolded protein binding
    		•  7.97191512372019 bayes_pls_golite062009
  • structural molecule activity
    		•  3.59589495899762 bayes_pls_golite062009
  • structural constituent of cytoskeleton
    		•  2.46121037473545 bayes_pls_golite062009
  • carbohydrate kinase activity
    		•  2.33338276303563 bayes_pls_golite062009
  • cytoskeletal protein binding
    		•  2.20983242331685 bayes_pls_golite062009
  • binding
    		•  2.11855816699924 bayes_pls_golite062009
  • protein binding
    		•  2.00711904074672 bayes_pls_golite062009
  • hydrolase activity
    		•  1.88613511243568 bayes_pls_golite062009
  • hexokinase activity
    		•  1.70516840340504 bayes_pls_golite062009
  • transcription regulator activity
    		•  1.68670098354938 bayes_pls_golite062009
  • nucleic acid binding
    		•  1.43834016708999 bayes_pls_golite062009
  • peptidase activity
    		•  1.4127499531612 bayes_pls_golite062009
  • DNA binding
    		•  1.36804228074586 bayes_pls_golite062009
  • transporter activity
    		•  1.2883466875237 bayes_pls_golite062009
  • phosphotransferase activity, alcohol group as acceptor
    		•  1.16749728168925 bayes_pls_golite062009
  • kinase activity
    		•  1.11102445353265 bayes_pls_golite062009
  • actin binding
    		•  1.03829083423378 bayes_pls_golite062009
  • transferase activity, transferring phosphorus-containing groups
    		•  0.953663805249074 bayes_pls_golite062009
  • hydrolase activity, acting on acid anhydrides
    		•  0.900931843471417 bayes_pls_golite062009
  • hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
    		•  0.898645342556399 bayes_pls_golite062009
  • pyrophosphatase activity
    		•  0.875671218597808 bayes_pls_golite062009
  • substrate-specific transporter activity
    		•  0.796517590178929 bayes_pls_golite062009
  • transcription factor activity
    		•  0.783439770973386 bayes_pls_golite062009
  • nucleoside-triphosphatase activity
    		•  0.78242344952806 bayes_pls_golite062009
  • protein transporter activity
    		•  0.780308299678282 bayes_pls_golite062009
  • ATPase activity
    		•  0.687771333492666 bayes_pls_golite062009
  • myosin binding
    		•  0.607451856096 bayes_pls_golite062009
  • glucose binding
    		•  0.53527958884988 bayes_pls_golite062009
  • glucokinase activity
    		•  0.47468456642723 bayes_pls_golite062009
  • catalytic activity
    		•  0.296484721581333 bayes_pls_golite062009
  • general RNA polymerase II transcription factor activity
    		•  0.186218331473028 bayes_pls_golite062009
  • chromatin binding
    		•  0.155140608043269 bayes_pls_golite062009
  • protease binding
    		•  0.121259015422625 bayes_pls_golite062009
  • chaperone binding
    		•  0.00111313452804307 bayes_pls_golite062009
    2 No functions predicted.
    3 No functions predicted.
    4 No functions predicted.
    5 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.91

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle