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Bait Protein: TOR1
Organism: Saccharomyces cerevisiae
Publication: Reinke A, Anderson S, McCaffery JM, Yates J 3rd, Aronova S, Chu S, Fairclough S, Iverson C, Wedaman KP, Powers T. TOR complex 1 includes a novel component, Tco89p (YPL180w), and cooperates with Ssd1p to maintain cellular integrity in Saccharomyces cerevisiae. J Biol Chem. 2004 Apr 9;279(15):14752-62. Epub 2004 Jan 21.
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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   IBI2 Protein of unknown function that associates with ribosomes; green fluorescent protein (GFP)-fusion p... 4 8 48.7% 0.0116 22179
[View Peptides]   SUP35 Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a domi... 30 50 48.0% 0.0209 76551
[View Peptides]   TOR1 PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in... 92 182 46.3% 0.0211 281138
[View Peptides]   DEF1 RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and prot... 38 106 43.0% 0.0411 83973
[View Peptides]   LST8 Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; ... 11 15 41.6% 0.0142 34034
[View Peptides]   TCO89 Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to... 23 27 40.7% 0.00967 88845
[View Peptides]   PDC1 Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... 11 13 39.3% 0.0066 61495
[View Peptides]   KOG1 Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or To... 53 131 38.4% 0.0241 177608
[View Peptides]   SSE1 ATPase that is a component of the heat shock protein Hsp90 chaperone complex; binds unfolded protein... 22 34 36.5% 0.014 77367
[View Peptides]   ENO2 Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... 10 14 33.2% 0.00916 46914
[View Peptides]   SPT5 Protein involved in regulating Pol I and Pol II transcription and pre-mRNA processing; forms a compl... 32 94 31.0% 0.0253 115650
[View Peptides]   SPT4 Protein involved in the regulating Pol I and Pol II transcription, pre-mRNA processing, kinetochore ... 4 6 30.4% 0.0168 11158
[View Peptides]   CMD1 Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... 3 6 29.9% 0.0117 16135
[View Peptides]   YPR137C-A, YAR010C Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 10 12 26.6% 0.0078 49072
[View Peptides]   YGR038C-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 10 12 26.6% 0.0078 49034
[View Peptides]   YGR027W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 10 12 26.6% 0.0078 49056
[View Peptides]   YER159C-A, YDR31... Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 10 12 26.6% 0.0078 49129
[View Peptides]   YDR365W-A, YPL25... Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 10 12 26.6% 0.0078 48953
[View Peptides]   YDR365W-A, YPL25... Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 10 12 26.6% 0.0078 48953
[View Peptides]   YER159C-A, YDR31... Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 10 12 26.6% 0.0078 49129
[View Peptides]   YDR261C-C Retrotransposon TYA Gag gene co-transcribed with TYB Pol; in YDRCTY1-3 TYB is mutant and probably no... 10 12 26.6% 0.0078 49009
[View Peptides]   YDR210C-C Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 10 12 26.6% 0.0078 49187
[View Peptides]   YDR098C-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 10 12 26.6% 0.0078 49057
[View Peptides]   FKS1 Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... 33 59 26.2% 0.009 214849
[View Peptides]   YDJ1 Protein chaperone involved in regulation of the HSP90 and HSP70 functions; involved in protein trans... 8 10 25.4% 0.00699 44671
[View Peptides]   FBA1 Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversio... 5 6 23.7% 0.00478 39621
[View Peptides]   BMH1 14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and D... 5 6 22.1% 0.00643 30091
[View Peptides]   BMH2 14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and D... 5 6 21.6% 0.00629 31061
[View Peptides]   RPP2B Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction ... 2 2 20.9% 0.0052 11050
[View Peptides]   GVP36 BAR domain-containing protein that localizes to both early and late Golgi vesicles; required for ada... 2 2 19.0% 0.00175 36670
[View Peptides]   TPI1 Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availabi... 2 2 18.5% 0.00231 26795
[View Peptides]   ACT1 Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functio... 3 4 18.4% 0.00305 41690
[View Peptides]   ADH1 Alcohol dehydrogenase, fermentative isozyme active as homo- or heterotetramers; required for the red... 4 4 18.4% 0.00329 36849
[View Peptides]   MLC1 Essential light chain for Myo1p, light chain for Myo2p; stabilizes Myo2p by binding to the neck regi... 2 2 14.8% 0.00384 16444
[View Peptides]   SAR1 GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for tra... 2 2 13.7% 0.00301 21451
[View Peptides]   VMA2 Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... 4 4 13.0% 0.00221 57749
[View Peptides]   LSB1 Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldri... 2 3 12.9% 0.00356 26139
[View Peptides]   RPP0 Conserved ribosomal protein P0 similar to rat P0, human P0, and E. coli L10e; shown to be phosphoryl... 3 5 12.8% 0.00458 33717
[View Peptides]   PGK1 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phospha... 3 4 12.5% 0.00275 44738
[View Peptides]   GSC2 Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore... 17 32 12.4% 0.00483 216988
[View Peptides]   SSC1 Hsp70 family ATPase, constituent of the import motor component of the Translocase of the Inner Mitoc... 5 7 12.2% 0.00306 70628
[View Peptides]   YHB1 Nitric oxide oxidoreductase, flavohemoglobin involved in nitric oxide detoxification; plays a role i... 2 2 10.8% 0.00143 44646
[View Peptides]   CDC19 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... 4 4 10.6% 0.00229 54545
[View Peptides]   YJL206C Putative protein of unknown function; similar to transcriptional regulators from the Zn[2]-Cys[6] bi... 5 7 10.4% 0.00264 86663
[View Peptides]   KAR2 ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... 5 5 10.0% 0.0021 74468
[View Peptides]   KAR2 ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... 5 5 10.0% 0.0021 74468
[View Peptides]   PIM1 ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required ... 6 8 9.4% 0.00202 127112
[View Peptides]   NSR1 Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ri... 2 2 9.4% 0.00138 44535
[View Peptides]   PHO84 High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated... 3 3 9.2% 0.00146 64382
[View Peptides]   PMA1 Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... 4 8 8.2% 0.00249 99619
[View Peptides]   EDC3 Non-essential conserved protein of unknown function, plays a role in mRNA decapping by specifically ... 2 2 8.2% 0.00104 61340
[View Peptides]   DED1 ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all ye... 3 3 7.5% 0.00142 65553
[View Peptides]   CYS4 Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the ... 2 2 7.5% 0.00113 56022
[View Peptides]   YDR261C-D Retrotransposon TYA Gag and TYB Pol genes; in YDRCTY1-3 TYB is mutant and probably non-functional 10 12 7.3% 0.00214 181660
[View Peptides]   HSC82 Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, a... 3 3 7.1% 0.00122 80900
[View Peptides]   NRP1 Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose de... 3 4 7.0% 0.00159 79299
[View Peptides]   TEF1, TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... 2 2 7.0% 0.00125 50033
[View Peptides]   TEF1, TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... 2 2 7.0% 0.00125 50033
[View Peptides]   FEN2 Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph 2 4 6.8% 0.00223 58257
[View Peptides]   YGR038C-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 10 12 6.7% 0.00196 198520
[View Peptides]   YGR027W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 10 12 6.7% 0.00196 198656
[View Peptides]   YER160C Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 10 12 6.7% 0.00196 198568
[View Peptides]   YER138C Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 10 12 6.7% 0.00196 198561
[View Peptides]   YDR365W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 10 12 6.7% 0.00196 198658
[View Peptides]   YDR316W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 10 12 6.7% 0.00196 198526
[View Peptides]   YDR210C-D Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 10 12 6.7% 0.00196 198838
[View Peptides]   YDR098C-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 10 12 6.7% 0.00196 198594
[View Peptides]   PDC5 Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruva... 2 2 6.7% 0.00102 61912
[View Peptides]   VPS13 Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated wit... 14 15 6.6% 0.00136 357850
[View Peptides]   TIF1, TIF2 Translation initiation factor eIF4A, identical to Tif2p; DEA(D/H)-box RNA helicase that couples ATPa... 2 2 6.6% 0.00145 44697
[View Peptides]   TIF1, TIF2 Translation initiation factor eIF4A, identical to Tif2p; DEA(D/H)-box RNA helicase that couples ATPa... 2 2 6.6% 0.00145 44697
[View Peptides]   TIM44 Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers ... 2 2 6.5% 0.00133 48854
[View Peptides]   TCP1 Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; inv... 2 3 6.4% 0.00153 60481
[View Peptides]   CRT10 Protein involved in transcriptional regulation of RNR2 and RNR3; expression of the gene is induced b... 3 3 6.1% 8.97E-4 109715
[View Peptides]   SSE2 Member of the heat shock protein 70 (HSP70) family; may be involved in protein folding; localized to... 3 6 5.9% 0.00248 77621
[View Peptides]   RNR1 One of two large regulatory subunits of ribonucleotide-diphosphate reductase; the RNR complex cataly... 3 3 5.5% 9.66E-4 99561
[View Peptides]   ECM10 Heat shock protein of the Hsp70 family, localized in mitochondrial nucleoids, plays a role in protei... 3 3 5.0% 0.00133 70085
[View Peptides]   SPA2 Component of the polarisome, which functions in actin cytoskeletal organization during polarized gro... 4 5 4.2% 9.76E-4 163142
[View Peptides]   DNF1 Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contribute... 3 3 4.1% 5.46E-4 177797
[View Peptides]   ASG1 Zinc cluster protein proposed to function as a transcriptional regulator involved in the stress resp... 2 2 4.1% 5.93E-4 108781
[View Peptides]   KAP123 Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and... 2 2 3.7% 5.14E-4 122601
[View Peptides]   CHS3 Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for ... 2 2 3.4% 4.91E-4 131601
[View Peptides]   KEL1 Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to ne... 2 2 3.0% 4.91E-4 131094
[View Peptides]   STE6 Plasma membrane ATP-binding cassette (ABC) transporter required for the export of a-factor, catalyze... 2 3 2.6% 6.65E-4 144765
[View Peptides]   DNF2 Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contribute... 2 2 1.6% 3.55E-4 182617
[View Peptides]   GCN1 Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn20p; proposed to stimulate ... 3 3 1.4% 3.21E-4 296697

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