Protein Overview: CKA1

General Information:
Name(s) found:	CKA1 / YIL035C [SGD]
Description(s) found: Found 30 descriptions. SHOW ALL	Alpha catalytic subunit of casein kinase 2, a Ser/Thr protein kinase with roles in cell growth and proliferation; the holoenzyme also contains CKA2, CKB1 and CKB2, the many substrates include transcription factors and all RNA polymerases [SGD]
Organism:	Saccharomyces cerevisiae
Length:	372 amino acids
Gene Ontology:
Cellular Component:	protein kinase CK2 complex [IDA]
Biological Process:	G2/M transition of mitotic cell cycle [IPI] G1/S transition of mitotic cell cycle [IPI] flocculation via cell wall protein-carbohydrate interaction [IMP] protein amino acid phosphorylation [IDA] response to DNA damage stimulus [IDA] regulation of transcription from RNA polymerase III promoter [IDA] regulation of transcription from RNA polymerase I promoter [IDA] cellular ion homeostasis [IPI]
Molecular Function:	protein kinase CK2 activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKCRVWSEAR VYTNINKQRT EEYWDYENTV IDWSTNTKDY EIENKVGRGK YSEVFQGVKL  60
   61 DSKVKIVIKM LKPVKKKKIK REIKILTDLS NEKVPPTTLP FQKDQYYTNQ KEDVLKFIRP 120
  121 YIFDQPHNGH ANIIHLFDII KDPISKTPAL VFEYVDNVDF RILYPKLTDL EIRFYMFELL 180
  181 KALDYCHSMG IMHRDVKPHN VMIDHKNKKL RLIDWGLAEF YHVNMEYNVR VASRFFKGPE 240
  241 LLVDYRMYDY SLDLWSFGTM LASMIFKREP FFHGTSNTDQ LVKIVKVLGT SDFEKYLLKY 300
  301 EITLPREFYD MDQYIRKPWH RFINDGNKHL SGNDEIIDLI DNLLRYDHQE RLTAKEAMGH 360
  361 PWFAPIREQI EK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CAB3 CDC73 CHD1 CKA1 CKA2 CKB1 CKB2 CTR9 HOT1 LEO1 PAF1 POB3 PSH1 RRP7 RTF1 SPT16 UTP22
View Details	(MIPS) Mewes HW, et al. (2004)		CKA1 CKA2 CKB1 CKB2
View Details	Krogan NJ, et al. (2006)		CAB3 CHD1 CKA1 CKA2 CKB1 CKB2 ELF1 HOT1 MSC3 NRG1 PGA2 REH1 RIM13 VHS3 YLR407W
View Details	Gavin AC, et al. (2006)		CKA1 CKA2 CKB1 CKB2
View Details	Gavin AC, et al. (2002)		ABF2 ACT1 ADE4 AIM31 ASC1 BFR2 BMH1 BMS1 BRX1 BUD21 CAB3 CBF5 CDC73 CFT1 CFT2 CHD1 CKA1 CKA2 CKB1 CKB2 CLP1 CMS1 CTI6 CTR9 DBP2 DBP8 DEP1 DIA4 DIM1 DIP2 DIS3 DOT6 EAF3 ECM16 ECM29 EMG1 ENO2 ENP1 ENP2 ERO1 ESF1 ESF2 FAP7 FIP1 GAL83 GCD6 GCN1 GLC7 HCA4 HHF1, HHF2 HOT1 HSL1 HTA1 HTA2 IML1 IMP3 IMP4 IOC3 ISW1 ISW2 KRE33 KRR1 LCP5 LEO1 LTV1 LYS12 MED4 MPE1 MPP10 MRD1 MRPS28 MVD1 NAN1 NHP10 NIP1 NOB1 NOC2 NOC4 NOP1 NOP14 NOP56 NOP58 NOP6 NRD1 NUP120 NUP145 NUP84 NUP85 PAF1 PAP1 PCF11 PFK1 PFS2 PHO23 PNO1 POB3 PRE6 PRE9 PRP43 PRT1 PTA1 PTI1 PWP2 RCL1 RCO1 REF2 REG1 RET1 RML2 RNA14 RNA15 ROK1 RPD3 RPG1 RPP2B RRP12 RRP7 RRP9 RSA3 RSC8 RTF1 RTG2 RVB2 RXT2 RXT3 SAK1 SAM1 SAP30 SCL1 SCP160 SDS3 SEC13 SEC16 SEC21 SEC23 SEC31 SEH1 SIN3 SIP1 SIP2 SLX9 SNF1 SNF4 SOF1 SPN1 SPT16 SPT6 SSA3 SSA4 SSU72 STM1 STO1 SYC1 TAF6 TIF1, TIF2 TIF35 TIF4632 TOP1 TSR1 TUP1 TYE7 UME1 UME6 UTP10 UTP11 UTP13 UTP15 UTP18 UTP20 UTP21 UTP22 UTP30 UTP4 UTP6 UTP7 UTP8 UTP9 VID24 VMR1 VPS1 VPS53 YCL046W YOR059C YSH1 YTA7 YTH1
View Details	Ho Y, et al. (2002)		ADH2 ADH4 BCY1 CDC28 CDC33 CEG1 CIC1 CKA1 CKA2 CKB1 CKB2 COP1 DBP10 DBP2 DIP2 DSS1 EGD1 ENP2 ERB1 ESF1 ESF2 ESL2 FKH1 FPR3 FYV8 GAL7 GCD2 GCD7 GCN3 GDE1 HAS1 HHF1, HHF2 HHT1, HHT2 HOT1 HTA1 HTB1 HTB2 IMD2 IMD3 IPP1 KRE33 KRI1 LAA1 LCP5 LHP1 MAM33 MBP1 MDH1 MGE1 MGM101 MPH1 MRPL1 MSS116 NAN1 NET1 NOC3 NOG1 NOP1 NOP12 NOP2 NOP4 NOP56 NOP7 NUG1 PDI1 PMA1 PNO1 POB3 POL2 PRP40 PTC5 PTC7 PUF6 PWP1 PWP2 RPF2 RRP1 RRP14 RRP5 SAH1 SEC2 SFP1 SIN3 SLD2 SPT16 SPT2 SRP1 SSF1 SUI2 SUI3 TAL1 TFP1 TIF4631 TIF6 TRL1 UBP12 URE2 UTP10 UTP11 UTP13 UTP14 UTP18 UTP21 UTP22 UTP30 UTP6 VMR1 VPS73 WTM2 YCR087W YDR102C YER084W YGR017W YMR144W YRA1
View Details	Qiu et al. (2008)		CKA1 CKA2 CKB1 CKB2

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	BOI2	Boi 2 gst control	McCusker D, et al (2007)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RHO3	CKA1	2	Drees BL, et al. (2001)
View Screen	CKB2	CKA1	2	Unpublished Fields Lab Data
View Screen	CKA2	CKA1	2	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	CKA1	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..13] [222..372]	PSI-BLAST	1107.0	Protein kinase CK2, alpha subunit
2	View Details	[14..31] [75..136] [155..221]	PSI-BLAST	1107.0	Protein kinase CK2, alpha subunit
3	View Details	[32..74] [137..154]	PSI-BLAST	1107.0	Protein kinase CK2, alpha subunit

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
phosphotransferase activity, alcohol group as acceptor	5.49988294224815	bayes_pls_golite062009
protein kinase activity	5.4586557943719	bayes_pls_golite062009
kinase activity	5.3287080791198	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	4.96052051118534	bayes_pls_golite062009
cyclin-dependent protein kinase activity	4.62392503670521	bayes_pls_golite062009
protein serine/threonine kinase activity	4.46381887831481	bayes_pls_golite062009
transferase activity	3.97138602499442	bayes_pls_golite062009
fibroblast growth factor receptor activity	3.64398689811167	bayes_pls_golite062009
protein tyrosine kinase activity	2.8153155526437	bayes_pls_golite062009
binding	2.74863538127007	bayes_pls_golite062009
transmembrane receptor activity	2.59526003830791	bayes_pls_golite062009
transmembrane receptor protein kinase activity	2.54265774272269	bayes_pls_golite062009
molecular transducer activity	2.40586892801446	bayes_pls_golite062009
signal transducer activity	2.40586892801446	bayes_pls_golite062009
receptor activity	2.39311927684508	bayes_pls_golite062009
structural molecule activity	2.32133082120229	bayes_pls_golite062009
protein binding	2.276111749106	bayes_pls_golite062009
ion transmembrane transporter activity	2.25301480460598	bayes_pls_golite062009
cation transmembrane transporter activity	2.188721764136	bayes_pls_golite062009
transmembrane receptor protein tyrosine kinase activity	2.16831387024372	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	2.12618184009486	bayes_pls_golite062009
receptor signaling protein activity	2.10379226653669	bayes_pls_golite062009
ATP binding	1.97600611746091	bayes_pls_golite062009
adenyl ribonucleotide binding	1.93596671856664	bayes_pls_golite062009
adenyl nucleotide binding	1.90928021591891	bayes_pls_golite062009
fibroblast growth factor binding	1.86067939172285	bayes_pls_golite062009
receptor signaling protein serine/threonine kinase activity	1.81249386965144	bayes_pls_golite062009
telethonin binding	1.75733591474207	bayes_pls_golite062009
fibroblast growth factor 1 binding	1.66976264488423	bayes_pls_golite062009
purine ribonucleotide binding	1.65732943334212	bayes_pls_golite062009
ribonucleotide binding	1.65726906484847	bayes_pls_golite062009
metal ion transmembrane transporter activity	1.65350201428136	bayes_pls_golite062009
purine nucleotide binding	1.63763394711025	bayes_pls_golite062009
nucleotide binding	1.63195828048121	bayes_pls_golite062009
transcription regulator activity	1.56748951434625	bayes_pls_golite062009
nucleic acid binding	1.46547714006072	bayes_pls_golite062009
JUN kinase kinase kinase activity	1.41172362253967	bayes_pls_golite062009
DNA binding	1.35333135345112	bayes_pls_golite062009
cytoskeletal protein binding	1.22172815474021	bayes_pls_golite062009
catalytic activity	1.21655087018101	bayes_pls_golite062009
transporter activity	1.11833481501707	bayes_pls_golite062009
transcription factor activity	1.10928254946881	bayes_pls_golite062009
phosphoinositide 3-kinase binding	1.02011129825141	bayes_pls_golite062009
transcription activator activity	0.997272622653026	bayes_pls_golite062009
transmembrane transporter activity	0.97575992765505	bayes_pls_golite062009
alpha-actinin binding	0.877087652539674	bayes_pls_golite062009
growth factor binding	0.86076618871384	bayes_pls_golite062009
acetylcholine receptor activator activity	0.85335678502604	bayes_pls_golite062009
cation channel activity	0.752655339263581	bayes_pls_golite062009
insulin receptor substrate binding	0.57867852584301	bayes_pls_golite062009
tubulin binding	0.525721160726465	bayes_pls_golite062009
protein serine/threonine/tyrosine kinase activity	0.48345772948903	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	0.47339115370427	bayes_pls_golite062009
enzyme binding	0.46284745266176	bayes_pls_golite062009
actin binding	0.456340967230669	bayes_pls_golite062009
substrate-specific transporter activity	0.451090007290896	bayes_pls_golite062009
magnesium ion binding	0.393328812780268	bayes_pls_golite062009
MAP kinase kinase kinase activity	0.37115026509016	bayes_pls_golite062009
MAP kinase kinase activity	0.35402045013148	bayes_pls_golite062009
structure-specific DNA binding	0.318153468064373	bayes_pls_golite062009
kinase binding	0.26752427107238	bayes_pls_golite062009
substrate-specific channel activity	0.26579358730346	bayes_pls_golite062009
RNA binding	0.245748631571068	bayes_pls_golite062009
protein complex binding	0.239339759110451	bayes_pls_golite062009
transmembrane receptor protein serine/threonine kinase activity	0.20643152368029	bayes_pls_golite062009
transforming growth factor beta receptor activity	0.20643152368029	bayes_pls_golite062009
microtubule binding	0.191960933574742	bayes_pls_golite062009
ligase activity, forming aminoacyl-tRNA and related compounds	0.149958560815481	bayes_pls_golite062009
ligase activity, forming carbon-oxygen bonds	0.149958560815481	bayes_pls_golite062009
aminoacyl-tRNA ligase activity	0.14842991990838	bayes_pls_golite062009
ubiquitin-protein ligase activity	0.114318824149111	bayes_pls_golite062009
MAP kinase activity	0.08504542461349	bayes_pls_golite062009
protein kinase binding	0.00674998152095019	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.0040394359628233	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2