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Protein Overview: SPT16

General Information:
Name(s) found: SPT16 / YGL207W [SGD]
Description(s) found:
Found 23 descriptions.
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  • Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 1035 amino acids

    Gene Ontology:
    Cellular Component: FACT complex [IGILink to PubMed article][IPILink to PubMed article]
    nuclear chromatin [IDALink to PubMed article]
    alpha DNA polymerase:primase complex [IDALink to PubMed article]
    transcription elongation factor complex [IPILink to PubMed article]
    Biological Process: transcription initiation from RNA polymerase II promoter [IDALink to PubMed article][IMPLink to PubMed article]
    RNA elongation from RNA polymerase II promoter [IPILink to PubMed article]
    Molecular Function: transcription elongation regulator activity [TASLink to PubMed article]
    RNA polymerase II transcription elongation factor activity [IPILink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MEELNIDFDV FKKRIELLYS KYNEFEGSPN SLLFVLGSSN AENPYQKTTI LHNWLLSYEF  60
   61 PATLIALVPG KVIIITSSAK AKHLQKAIDL FKDPESKITL ELWQRNNKEP ELNKKLFDDV 120
  121 IALINSAGKT VGIPEKDSYQ GKFMTEWNPV WEAAVKENEF NVIDISLGLS KVWEVKDVNE 180
  181 QAFLSVSSKG SDKFMDLLSN EMVRAVDEEL KITNAKLSDK IENKIDDVKF LKQLSPDLSA 240
  241 LCPPNYKFNF DLLDWTYSPI IQSGKKFDLR VSARSTNDQL YGNGCILASC GIRYNNYCSN 300
  301 ITRTFLIDPS EEMANNYDFL LTLQKEIVTN ILKPGRTPKE VYESVIEYIE KTKPELVPNF 360
  361 TKNIGSLIGL EFRDSNFILN VKNDYRKIQR GDCFNISFGF NNLKDSQSAN NYALQLADTV 420
  421 QIPLDETEPP RFLTNYTKAK SQISFYFNNE EEDNNKKKSS PATKVPSKPD RNSKILRTKL 480
  481 RGEARGGAED AQKEQIRKEN QKKLHEKLEK NGLLRFSAAD ANGPDSEPRQ YFKKYESYVR 540
  541 DSQLPTNIRD LRIHVDWKSQ TIILPIYGRP VPFHINSYKN GSKNEEGEYT YLRLNFNSPG 600
  601 SSGGISKKVE ELPYEESADN QFVRSITLRS KDGDRMSETF KQIADLKKEA TKREQERKAL 660
  661 ADVVQQDKLI ENKTGRTKRL DQIFVRPNPD TKRVPSTVFI HENGIRFQSP LRTDSRIDIL 720
  721 FSNIKNLIFQ SCKGELIVVI HIHLKNPILM GKKKIQDVQF YREASDMSVD ETGGGRRGQS 780
  781 RFRRYGDEDE LEQEQEERRK RAALDKEFKY FADAIAEASN GLLTVENTFR DLGFQGVPNR 840
  841 SAVFCMPTTD CLVQLIEPPF LVINLEEVEI CILERVQFGL KNFDMVFVYK DFNKPVTHIN 900
  901 TVPIESLDFL KQWLTDMDIP YTVSTINLNW ATIMKSLQDD PYQFFLDGGW NFLATGSDDE 960
  961 ASDESEEEVS EYEASEDDVS DESAFSEDEE GSEVDDDISG DESEDYTGDE SEEGEDWDEL1020
 1021 EKKAARADRG ANFRD

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) CDC73 CHD1 CKA1 CKA2 CKB1 CKB2 CTR9 HAT1 HAT2 HIF1 KAP114 LEO1 MAM33 PAF1 POB3 PSH1 RTF1 SPT16
    View Details Krogan NJ, et al. (2006) ARA2 AYT1 BRO1 CDC73 CTR9 GLO4 HHT1, HHT2 LEO1 PAF1 PML39 POB3 PSH1 RTF1 SEN1 SPT16 YHR035W
    View Details Gavin AC, et al. (2002) AMN1 AVO1 BEM2 CAB3 CDC73 CEG1 CET1 CHD1 CKA1 CKA2 CKB1 CKB2 CLU1 CTR9 ESP1 GTR2 HHF1, HHF2 HOT1 IML1 ISW1 ISW2 LEO1 LSM12 LYS12 NIP1 NOP1 PAF1 PDB1 POB3 PRP43 PRT1 RET1 RPG1 RTF1 RVB2 SPT16 SSA3 SSA4 TIF35 TOP2 UTP22 VPS1 YCH1 YCR015C YTA7
    View Details Ho Y, et al. (2002) ACO1 AIM13 CIC1 CKA1 CKA2 CKB1 CKB2 CLU1 DBP10 DBP2 EDE1 EGD1 ENP2 ERB1 FPR3 GAC1 GDE1 HAS1 HHF1, HHF2 HHT1, HHT2 HIR2 HOT1 HTA1 HTA2 HTB1 HTB2 KAP114 KNS1 KRE33 KRI1 MAM33 MER1 MGM101 MRPL1 NAP1 NOB1 NOC3 NOG1 NOP12 NOP2 NOP4 NOP56 NOP7 NUG1 NUM1 PAA1 PDI1 POB3 POL2 PSH1 PTC4 PUF6 PWP1 RET1 RPC82 RPO31 RRP14 RRP5 SEF1 SFP1 SKT5 SPT16 SSF1 SYF1 SYP1 TIF4631 TIF6 TOP1 TPS1 TRL1 UTP13 UTP22 VHS1 VMR1 VPS73 WTM2 YER084W YRA1
    View Details Qiu et al. (2008) CDC73 CHD1 ELA1 ELC1 ELP2 ELP6 IKI1 PAF1 POB3 RTF1 SPN1 SPT16 SPT4 SPT5 TFB5

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run BOI1 Sample boi 1 with ha tag from october 2005 McCusker D, et al (2007)
    View Run MLP2 #04 Alpha Factor Prep2 Keck JM, et al. (2011)
    View Run MLP2 #13 Mitotic Prep2-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #10 Mitotic Prep1-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data SPT16 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..171] PSI-BLAST 80.045757 Aminopeptidase P; Aminopeptidase P, C-terminal domain
    2 View Details [172..468] PSI-BLAST 80.045757 Aminopeptidase P; Aminopeptidase P, C-terminal domain
    3 View Details [469..523] deduced N/A No confident structure predictions are available.
    4 View Details [524..773] MSA 1.016941 View MSA. No confident structure predictions are available.
    5 View Details [774..918] deduced N/A No confident structure predictions are available.
    6 View Details [919..1035] MSA 3.018996 View MSA. No confident structure predictions are available.

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • transcription regulator activity
    		•  2.3228898238998 bayes_pls_golite062009
  • DNA binding
    		•  1.6830709273154 bayes_pls_golite062009
  • nucleic acid binding
    		•  1.67660713848062 bayes_pls_golite062009
  • binding
    		•  1.4562583231987 bayes_pls_golite062009
  • transcription factor activity
    		•  1.02226978078776 bayes_pls_golite062009
  • protein binding
    		•  0.170797254754318 bayes_pls_golite062009
  • catalytic activity
    		•  0.148551873544128 bayes_pls_golite062009
    2 No functions predicted.
    3 No functions predicted.
    4 No functions predicted.
    5 No functions predicted.
    6 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.95

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle