Protein Overview: NOB1

General Information:
Name(s) found:	NOB1 / YOR056C [SGD]
Description(s) found: Found 30 descriptions. SHOW ALL	Essential nuclear protein involved in proteasome maturation and synthesis of 40S ribosomal subunits; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA [SGD]
Organism:	Saccharomyces cerevisiae
Length:	459 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] preribosome [IDA][IEP][IPI]
Biological Process:	maturation of SSU-rRNA [IEP][IMP ] ribosomal small subunit biogenesis [IPI] ribosome biogenesis [RCA] proteasome assembly [IMP][IPI]
Molecular Function:	RNA binding [IDA][ISS] protein binding [IDA][IMP]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTENQTAHVR ALILDATPLI TQSYTHYQNY AQSFYTTPTV FQEIKDAQAR KNLEIWQSLG  60
   61 TLKLVHPSEN SIAKVSTFAK LTGDYSVLSA NDLHILALTY ELEIKLNNGD WRLRKKPGDA 120
  121 LDASKADVGT DGKQKLTEDN KKEEDSESVP KKKNKRRGGK KQKAKREARE AREAENANLE 180
  181 LESKAEEHVE EAGSKEQICN DENIKESSDL NEVFEDADDD GDWITPENLT EAIIKDSGED 240
  241 TTGSLGVEAS EEDRHVALNR PENQVALATG DFAVQNVALQ MNLNLMNFMS GLKIKRIRNY 300
  301 MLRCHACFKI FPLPKDGKPK HFCASCGGQG TLLRCAVSVD SRTGNVTPHL KSNFQWNNRG 360
  361 NRYSVASPLS KNSQKRYGKK GHVHSKPQEN VILREDQKEY EKVIKQEEWT RRHNEKILNN 420
  421 WIGGGSADNY ISPFAITGLK QHNVRIGKGR YVNSSKRRS

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ENP1 LTV1 NOB1 RIO2 TSR1
View Details	Krogan NJ, et al. (2006)		BUD22 CIC1 DBP6 EFG1 FRE3 GIS2 HAS1 MAK21 NIP7 NOB1 NOC2 NOC3 NOP1 NOP2 NOP8 PUS7 RRP5 RSA3 SBP1 SFA1 SSQ1 YGR272C YMR034C YRF1-8, YRF1-5, ...
View Details	Gavin AC, et al. (2006)		HRR25 LTV1 NOB1 RIO2 TSR1
View Details	Gavin AC, et al. (2002)		ADE4 ADH1 ARP9 ASC1 BEM2 BFR2 BMS1 BRX1 BUD21 CBF5 CKA1 CKA2 CKB1 CKB2 CMS1 DBP8 DIA4 DIM1 DIP2 DIS3 ECM16 ECM29 EMG1 ENP1 ENP2 ERO1 ESF1 ESF2 FAP7 GCN1 HCA4 HHF1, HHF2 HRR25 HSL1 HTA2 IMP3 IMP4 KRE33 KRR1 LCP5 LTV1 MPP10 MRD1 MVD1 NAN1 NOB1 NOC2 NOC4 NOP1 NOP14 NOP56 NOP58 NOP6 PNO1 PRE6 PRE9 PRP43 PWP2 RCL1 RIO2 RML2 ROK1 RPP2B RRP12 RRP7 RRP9 RTG2 SAM1 SCL1 SEC21 SLX9 SNF12 SNF2 SNF5 SNF6 SOF1 SWI1 SWI3 SWP82 TIF1, TIF2 TSR1 UTP10 UTP11 UTP13 UTP15 UTP18 UTP20 UTP21 UTP22 UTP30 UTP4 UTP6 UTP7 UTP8 UTP9 VPS1 YGR054W YOR059C
View Details	Ho Y, et al. (2002)		ASC1 EDE1 ENP2 GAC1 HHF1, HHF2 HTA1 HTA2 HTB1 HTB2 KNS1 KRI1 MAM33 NOB1 NOP12 NOP56 NUG1 POB3 PRP43 PSH1 RFA1 SPT16 SYP1 TSR1
View Details	Qiu et al. (2008)		ENP1 LTV1 NOB1 NOC3 NOC4 NOP14 PWP2

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

Searching the YRC Public Image Repository, found results:

Currently showing to of results.

[View Search in the YRC Public Image Repository]

Searching the YRC Public Image Repository, found no results.

Ginzu Version Information

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

Gene Ontology predictions

Term	Confidence	Notes
nuclease activity	2.0989149357263	bayes_pls_golite062009
DNA binding	1.98330542087379	bayes_pls_golite062009
binding	1.91435224223408	bayes_pls_golite062009
nucleic acid binding	1.86243238793767	bayes_pls_golite062009
exonuclease activity	1.7620419369992	bayes_pls_golite062009
endodeoxyribonuclease activity	1.74349203224875	bayes_pls_golite062009
deoxyribonuclease activity	1.68760157046614	bayes_pls_golite062009
endonuclease activity	1.2840172881923	bayes_pls_golite062009
catalytic activity	1.27951740231695	bayes_pls_golite062009
flap endonuclease activity	1.17726395799783	bayes_pls_golite062009
endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	1.07360773846975	bayes_pls_golite062009
5'-3' exonuclease activity	0.974028111038185	bayes_pls_golite062009
hydrolase activity, acting on ester bonds	0.867731336605067	bayes_pls_golite062009
hydrolase activity	0.827276601361763	bayes_pls_golite062009
exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	0.79417076649627	bayes_pls_golite062009
protein binding	0.695466920831325	bayes_pls_golite062009
exodeoxyribonuclease activity, producing 5'-phosphomonoesters	0.267787854683683	bayes_pls_golite062009
exodeoxyribonuclease activity	0.260397221344929	bayes_pls_golite062009

No functions predicted.

Term	Confidence	Notes
RNA binding	2.67504868533195	bayes_pls_golite062009
binding	2.1226043957694	bayes_pls_golite062009
nucleic acid binding	1.26170105245043	bayes_pls_golite062009
protein binding	0.874974846355882	bayes_pls_golite062009
catalytic activity	0.316629025709297	bayes_pls_golite062009

No functions predicted.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.97

Source: Reynolds et al. (2008)

	PROTEIN(S)	PUBLICATION
View Data	NOB1 and SPC42	Unpublished Data
View Data	NOB1	Huh WK, et al. (2003)

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..177]	MSA	15.041993	View MSA. No confident structure predictions are available.
2	View Details	[178..459]	MSA	1.015926	View MSA. No confident structure predictions are available.
3	View Details	[223..386]	PSI-BLAST	23.0	Solution Structure of RSGI RUH-035, a Zn-ribbon module in Mouse cDNA
4	View Details	[387..459]	deduced	N/A	No confident structure predictions are available.

Protein Overview: NOB1

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: