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Protein Overview: RPO31

General Information:
Name(s) found: RPO31 / YOR116C [SGD]
Description(s) found:
Found 30 descriptions.
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  • RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 1460 amino acids

    Gene Ontology:
    Cellular Component: DNA-directed RNA polymerase III complex [TASLink to PubMed article]
    Biological Process: transcription from RNA polymerase III promoter [TAS]
    Molecular Function: DNA-directed RNA polymerase activity [TASLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKEVVVSETP KRIKGLEFSA LSAADIVAQS EVEVSTRDLF DLEKDRAPKA NGALDPKMGV  60
   61 SSSSLECATC HGNLASCHGH FGHLKLALPV FHIGYFKATI QILQGICKNC SAILLSETDK 120
  121 RQFLHELRRP GVDNLRRMGI LKKILDQCKK QRRCLHCGAL NGVVKKAAAG AGSAALKIIH 180
  181 DTFRWVGKKS APEKDIWVGE WKEVLAHNPE LERYVKRCMD DLNPLKTLNL FKQIKSADCE 240
  241 LLGIDATVPS GRPETYIWRY LPAPPVCIRP SVMMQDSPAS NEDDLTVKLT EIVWTSSLIK 300
  301 AGLDKGISIN NMMEHWDYLQ LTVAMYINSD SVNPAMLPGS SNGGGKVKPI RGFCQRLKGK 360
  361 QGRFRGNLSG KRVDFSGRTV ISPDPNLSID EVAVPDRVAK VLTYPEKVTR YNRHKLQELI 420
  421 VNGPNVHPGA NYLLKRNEDA RRNLRYGDRM KLAKNLQIGD VVERHLEDGD VVLFNRQPSL 480
  481 HRLSILSHYA KIRPWRTFRL NECVCTPYNA DFDGDEMNLH VPQTEEARAE AINLMGVKNN 540
  541 LLTPKSGEPI IAATQDFITG SYLISHKDSF YDRATLTQLL SMMSDGIEHF DIPPPAIMKP 600
  601 YYLWTGKQVF SLLIKPNHNS PVVINLDAKN KVFVPPKSKS LPNEMSQNDG FVIIRGSQIL 660
  661 SGVMDKSVLG DGKKHSVFYT ILRDYGPQEA ANAMNRMAKL CARFLGNRGF SIGINDVTPA 720
  721 DDLKQKKEEL VEIAYHKCDE LITLFNKGEL ETQPGCNEEQ TLEAKIGGLL SKVREEVGDV 780
  781 CINELDNWNA PLIMATCGSK GSTLNVSQMV AVVGQQIISG NRVPDGFQDR SLPHFPKNSK 840
  841 TPQSKGFVRN SFFSGLSPPE FLFHAISGRE GLVDTAVKTA ETGYMSRRLM KSLEDLSCQY 900
  901 DNTVRTSANG IVQFTYGGDG LDPLEMEGNA QPVNFNRSWD HAYNITFNNQ DKGLLPYAIM 960
  961 ETANEILGPL EERLVRYDNS GCLVKREDLN KAEYVDQYDA ERDFYHSLRE YINGKATALA1020
 1021 NLRKSRGMLG LLEPPAKELQ GIDPDETVPD NVKTSVSQLY RISEKSVRKF LEIALFKYRK1080
 1081 ARLEPGTAIG AIGAQSIGEP GTQMTLKTFH FAGVASMNVT LGVPRIKEII NASKVISTPI1140
 1141 INAVLVNDND ERAARVVKGR VEKTLLSDVA FYVQDVYKDN LSFIQVRIDL GTIDKLQLEL1200
 1201 TIEDIAVAIT RASKLKIQAS DVNIIGKDRI AINVFPEGYK AKSISTSAKE PSENDVFYRM1260
 1261 QQLRRALPDV VVKGLPDISR AVINIRDDGK RELLVEGYGL RDVMCTDGVI GSRTTTNHVL1320
 1321 EVFSVLGIEA ARYSIIREIN YTMSNHGMSV DPRHIQLLGD VMTYKGEVLG ITRFGLSKMR1380
 1381 DSVLQLASFE KTTDHLFDAA FYMKKDAVEG VSECIILGQT MSIGTGSFKV VKGTNISEKD1440
 1441 LVPKRCLFES LSNEAALKAN

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) ISW2 MOT1 RET1 RPA135 RPA190 RPB10 RPB5 RPB8 RPC11 RPC17 RPC19 RPC25 RPC31 RPC34 RPC37 RPC40 RPC53 RPC82 RPO26 RPO31
    View Details (MIPS) Mewes HW, et al. (2004) RET1 RPB10 RPB5 RPB8 RPC10 RPC19 RPC25 RPC31 RPC34 RPC40 RPC82 RPO26 RPO31
    View Details Krogan NJ, et al. (2006) BDP1 CDC14 COY1 FAR7 MAF1 RET1 RPA12 RPA135 RPA14 RPA190 RPA34 RPA43 RPA49 RPC10 RPC11 RPC17 RPC19 RPC25 RPC31 RPC34 RPC37 RPC40 RPC53 RPC82 RPO31 YDL144C YIR024C
    View Details Gavin AC, et al. (2006) RET1 RPC25 RPC31 RPC34 RPC37 RPC40 RPC53 RPC82 RPO31
    View Details Gavin AC, et al. (2002) ADE5,7 AMS1 ASC1 BNI1 CDC14 CDC48 DHH1 DOA1 DPB2 DPB4 FBA1 HHF1, HHF2 IOC3 ISW1 ISW2 ITC1 KAP114 MAF1 MOT1 MRPS28 NAP1 NPL4 NPL6 PDC1 POL2 PUF6 RAI1 REB1 REG1 RET1 RFA1 RFX1 RNR2 RPA12 RPA135 RPA190 RPA43 RPA49 RPB10 RPB5 RPB8 RPC11 RPC17 RPC19 RPC25 RPC31 RPC34 RPC37 RPC40 RPC53 RPC82 RPO26 RPO31 RSC2 RSC3 RSC58 RSC8 RVB2 SFH1 SHP1 SRO7 STH1 STO1 TAF1 TFC7 TOP1 UFD1 VPS1 YBR090C-A, NHP6B YDR049W
    View Details Ho Y, et al. (2002) ACC1 ACH1 ADH2 ADR1 ARP4 DOP1 ECM29 FAA2 GCD11 GFA1 GND1 GUS1 HFI1 HHF1, HHF2 HIR2 HTA1 HTB1 IDH2 ISA2 KAP114 KAP122 KRI1 MDH1 MET18 NAP1 NGL2 NOP4 NOP7 POL30 PYC1 PYC2 RET1 RPA135 RPA190 RPA49 RPB5 RPC19 RPC25 RPC34 RPC40 RPC82 RPN3 RPO26 RPO31 RVS167 SEC27 SPT16 SPT7 SRY1 STB4 SWC4 SWI5 TBS1 TFP1 TRA1 TSA2 UTP13 YER138C YFL042C YHR112C
    View Details Qiu et al. (2008) RET1 RPA12 RPA135 RPB10 RPB11 RPB3 RPB5 RPB7 RPB8 RPB9 RPC10 RPC11 RPC17 RPC19 RPC25 RPC31 RPC34 RPC37 RPC40 RPC53 RPC82 RPO21 RPO26 RPO31

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run CEP3 sample cbf3 from feb 2005 Sandall S, et all (2006)
    View Run ZDS1 Sample 3 - cpn + atp, from june 2005 McCusker D, et al (2007)
    View Run MLP2 #31 Asynchronous Prep (Protease cleavage) Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

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      PROTEIN(S) PUBLICATION
    View Data RPO31 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..1460] PSI-BLAST 11000.0 RBP1


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.95

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle