Protein Overview: NOG1

General Information:
Name(s) found:	NOG1 / YPL093W [SGD]
Description(s) found: Found 26 descriptions. SHOW ALL	Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins [SGD]
Organism:	Saccharomyces cerevisiae
Length:	647 amino acids
Gene Ontology:
Cellular Component:	nuclear outer membrane [IMP] nucleolus [IDA] preribosome, large subunit precursor [IDA]
Biological Process:	ribosomal subunit export from nucleus [IMP][IPI] ribosomal large subunit biogenesis [IDA] ribosome biogenesis [RCA]
Molecular Function:	GTP binding [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MQLSWKDIPT VAPANDLLDI VLNRTQRKTP TVIRPGFKIT RIRAFYMRKV KYTGEGFVEK  60
   61 FEDILKGFPN INDVHPFHRD LMDTLYEKNH YKISLAAISR AKSLVEQVAR DYVRLLKFGQ 120
  121 SLFQCKQLKR AALGRMATIV KKLRDPLAYL EQVRQHIGRL PSIDPNTRTL LICGYPNVGK 180
  181 SSFLRCITKS DVDVQPYAFT TKSLYVGHFD YKYLRFQAID TPGILDRPTE EMNNIEMQSI 240
  241 YAIAHLRSCV LYFMDLSEQC GFTIEAQVKL FHSIKPLFAN KSVMVVINKT DIIRPEDLDE 300
  301 ERAQLLESVK EVPGVEIMTS SCQLEENVME VRNKACEKLL ASRIENKLKS QSRINNVLNK 360
  361 IHVAQPQARD DVKRTPFIPE SVKNLKKYDP EDPNRRKLAR DIEAENGGAG VFNVNLKDKY 420
  421 LLEDDEWKND IMPEILDGKN VYDFLDPEIA AKLQALEEEE EKLENEGFYN SDDEEEIYDG 480
  481 FEASEVDDIK EKAAWIRNRQ KTMIAEARNR KSLKNKAIMP RSKLTKSFGK MEEHMSTLGH 540
  541 DMSALQDKQN RAARKNRYVE RGSDVVFGDQ DALTASTENG VKLRQTDRLL DGVADGSMRS 600
  601 KADRMAKMER RERNRHAKQG ESDRHNAVSL SKHLFSGKRG VGKTDFR

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ARX1 BRX1 BUD20 CIC1 DBP10 DBP9 DRS1 EBP2 ERB1 FPR4 HAS1 IPI3 LSG1 MAK21 MDN1 MRT4 NIP7 NOC2 NOC3 NOG1 NOG2 NOP12 NOP15 NOP2 NOP4 NOP7 NSA1 NSA2 NUG1 PUF6 RIX1 RIX7 RLP7 RPF1 RPF2 RPL1A, RPL1B RPL23B, RPL23A RPL25 RPL27A RPL28 RPL36B RPL38 RPL6A RPL9A RRP12 RSA4 SDA1 SPB1 SPB4 TIF6 URB1 YTM1
View Details	Krogan NJ, et al. (2006)		NOG1 NOG2 NOP15 NUG1 YSW1
View Details	Gavin AC, et al. (2006)		ARX1 BUD20 FPR4 IPI3 MRT4 NIP7 NOG1 NOG2 NOP2 NSA2 NUG1 RIX1 RPF2 RSA4 SDA1 TIF6
View Details	Gavin AC, et al. (2002)		AAR2 ADE3 ARX1 ASC1 ATG9 BEM2 BMS1 BRX1 BUD20 CBF5 CIC1 CLU1 CYR1 CYS4 DBP10 DBP2 DBP9 DCP2 DRS1 EBP2 ERB1 FPR4 FUN12 GAR1 GCN1 GLT1 HAS1 HCR1 HDA1 HER1 HIS3 INP2 IPI1 IPI3 IST2 KRE33 KRR1 KTR5 LHP1 LOC1 LSG1 MAK21 MAK5 MDN1 MIS1 MRP7 MRT4 NAP1 NHP2 NIP1 NIP7 NMD3 NOC2 NOC3 NOG1 NOG2 NOP1 NOP12 NOP15 NOP16 NOP2 NOP4 NOP56 NOP6 NOP7 NSA1 NSA2 NUG1 POL2 PRP4 PRT1 PUF6 RAT1 REI1 RIC1 RIX1 RIX7 RLI1 RLP24 RLP7 RPA135 RPA190 RPF1 RPF2 RPG1 RPP2B RPT6 RRP1 RRP12 RSA3 RSA4 RTR1 SDA1 SEC7 SHM1 SKI2 SMB1 SNU114 SNU13 SNU66 SPB1 SPB4 SPT5 SRO9 SSF1 SUA7 SUI1 SUI2 SUI3 SUP45 TIF34 TIF35 TIF4631 TIF4632 TIF5 TIF6 TUB3 URB1 UTP10 UTP20 UTP21 UTP22 UTP6 YGL036W YMR310C YPL009C YTM1
View Details	Ho Y, et al. (2002)		ACO1 AFG2 ALB1 APL2 APL4 APM1 ARX1 BRX1 BUD20 CCT6 CDC14 CIC1 CKA1 CKB1 CKB2 DBP2 DSS4 EBP2 EGD1 EGD2 EHD3 ELA1 ENP2 ERB1 EXG1 FAA4 FPR3 FPR4 FYV4 GND1 GUS1 HAS1 HHF1, HHF2 HHT1, HHT2 HOT1 HTA1 HTB1 HTB2 HXT7 IMD3 IMD4 KRE33 KRI1 KRR1 LHP1 MAM33 MET6 MGM101 MNP1 MRPS16 MRT4 MSS116 MUB1 NMA1 NMD3 NOC2 NOC3 NOG1 NOP1 NOP12 NOP13 NOP15 NOP16 NOP2 NOP4 NOP56 NOP7 NSA1 NSA2 NUG1 PAB1 PDI1 PET127 PIB2 POB3 PRP43 PUF6 PWP1 RCL1 RIX1 RLP7 RPF2 RRP12 RRP17 RSM24 RSM25 SAH1 SCS2 SEC4 SEC53 SPB4 SPT16 SSF1 SSQ1 THI21 TIF6 TIS11 TUF1 UBR2 YBL044W YER077C YER084W YPT1 YTM1
View Details	Qiu et al. (2008)		CIC1 NOG1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MIH1	control 1	McCusker D, et al (2007)
View Run	MIH1	control 2	McCusker D, et al (2007)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi1 with gst from october 2005	McCusker D, et al (2007)
View Run	VAM3	Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control).	Hao Xu, et al. (2010)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	NOG1	SPC72	2	Unpublished Fields Lab Data
View Screen	NOG1	CKB2	2	Unpublished Fields Lab Data
View Screen	NOG1	KIN3	2	Unpublished Fields Lab Data
View Screen	NOG1	CDC21	2	Unpublished Fields Lab Data
View Screen	NOG1	FRT1	2	Unpublished Fields Lab Data
View Screen	NOG1	CSM1	2	Unpublished Fields Lab Data
View Screen	NOG1	AIR2	2	Unpublished Fields Lab Data
View Screen	NOG1	FOB1	2	Unpublished Fields Lab Data
View Screen	NOG1	MCM6	2	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	NOG1	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..161]	PSI-BLAST	64.09691	Probable GTPase Der, N-terminal and middle domains; Probable GTPase Der, C-terminal domain
2	View Details	[162..344]	PSI-BLAST	64.09691	Probable GTPase Der, N-terminal and middle domains; Probable GTPase Der, C-terminal domain
3	View Details	[345..440]	PSI-BLAST	64.09691	Probable GTPase Der, N-terminal and middle domains; Probable GTPase Der, C-terminal domain
4	View Details	[441..647]	MSA	2.205961	View MSA. No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
nucleoside-triphosphatase activity	2.85628130885095	bayes_pls_golite062009
pyrophosphatase activity	2.78950165917982	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	2.76996223962376	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	2.7670079419026	bayes_pls_golite062009
binding	2.64537357529343	bayes_pls_golite062009
microtubule motor activity	2.61518930814923	bayes_pls_golite062009
motor activity	2.60481158518321	bayes_pls_golite062009
transporter activity	2.36978240084485	bayes_pls_golite062009
ATPase activity	2.26864012421561	bayes_pls_golite062009
nucleic acid binding	2.21184938313192	bayes_pls_golite062009
transmembrane transporter activity	2.18065576425044	bayes_pls_golite062009
ATPase activity, coupled	2.07184330056272	bayes_pls_golite062009
DNA binding	1.94055378599526	bayes_pls_golite062009
transcription regulator activity	1.83584389083042	bayes_pls_golite062009
structural constituent of ribosome	1.80055405467543	bayes_pls_golite062009
helicase activity	1.70242731830224	bayes_pls_golite062009
RNA binding	1.60613159109369	bayes_pls_golite062009
ribonucleotide binding	1.53142082854758	bayes_pls_golite062009
purine nucleotide binding	1.52938239070386	bayes_pls_golite062009
purine ribonucleotide binding	1.52887308780164	bayes_pls_golite062009
nucleotide binding	1.52746968836881	bayes_pls_golite062009
ubiquitin-protein ligase activity	1.52494005252804	bayes_pls_golite062009
hydrolase activity	1.44476395406467	bayes_pls_golite062009
	1.44426272235796	bayes_pls_golite062009
RNA helicase activity	1.42791449104435	bayes_pls_golite062009
RNA-dependent ATPase activity	1.39335733374328	bayes_pls_golite062009
ATP-dependent RNA helicase activity	1.36724390116598	bayes_pls_golite062009
microfilament motor activity	1.28029120876672	bayes_pls_golite062009
small conjugating protein ligase activity	1.25258316272121	bayes_pls_golite062009
ATP binding	1.23942044024113	bayes_pls_golite062009
DNA-dependent ATPase activity	1.2155186842031	bayes_pls_golite062009
adenyl ribonucleotide binding	1.21044170258803	bayes_pls_golite062009
adenyl nucleotide binding	1.19481945569892	bayes_pls_golite062009
purine NTP-dependent helicase activity	1.19311444180077	bayes_pls_golite062009
ATP-dependent helicase activity	1.19311444180077	bayes_pls_golite062009
substrate-specific transporter activity	1.09373202836575	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
microtubule binding	0.889065347963087	bayes_pls_golite062009
structural molecule activity	0.86163779946438	bayes_pls_golite062009
cytoskeletal protein binding	0.832861637931298	bayes_pls_golite062009
tubulin binding	0.818881964708536	bayes_pls_golite062009
DNA polymerase activity	0.795745617990735	bayes_pls_golite062009
structure-specific DNA binding	0.782430219754236	bayes_pls_golite062009
DNA-directed DNA polymerase activity	0.771854032852428	bayes_pls_golite062009
acid-amino acid ligase activity	0.736988322066518	bayes_pls_golite062009
DNA helicase activity	0.72881469450553	bayes_pls_golite062009
transcription factor activity	0.697256579145077	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	0.696000326143746	bayes_pls_golite062009
transcription repressor activity	0.677612020025357	bayes_pls_golite062009
protein-DNA loading ATPase activity	0.650964453464311	bayes_pls_golite062009
hydrogen ion transporting ATP synthase activity, rotational mechanism	0.643562482062891	bayes_pls_golite062009
kinase activity	0.641541011250583	bayes_pls_golite062009
double-stranded DNA binding	0.6071273686445	bayes_pls_golite062009
ATP-dependent DNA helicase activity	0.5886142102548	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.584453361662212	bayes_pls_golite062009
DNA clamp loader activity	0.543512707823441	bayes_pls_golite062009
GTPase activity	0.437665353870877	bayes_pls_golite062009
active transmembrane transporter activity	0.434775387083003	bayes_pls_golite062009
ligase activity, forming carbon-nitrogen bonds	0.39380930211325	bayes_pls_golite062009
actin-dependent ATPase activity	0.36110509588725	bayes_pls_golite062009
protein kinase activity	0.351308564667977	bayes_pls_golite062009
primary active transmembrane transporter activity	0.294474570607914	bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity	0.282352478532746	bayes_pls_golite062009
actin binding	0.269954671912367	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	0.220608508617331	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of substances	0.219533217661421	bayes_pls_golite062009
ATPase activity, coupled to movement of substances	0.219157471322311	bayes_pls_golite062009
protein binding	0.170837278876362	bayes_pls_golite062009
ion transmembrane transporter activity	0.140475613064199	bayes_pls_golite062009

Term	Confidence	Notes
nucleoside-triphosphatase activity	2.85628130885095	bayes_pls_golite062009
pyrophosphatase activity	2.78950165917982	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	2.76996223962376	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	2.7670079419026	bayes_pls_golite062009
binding	2.64537357529343	bayes_pls_golite062009
microtubule motor activity	2.61518930814923	bayes_pls_golite062009
motor activity	2.60481158518321	bayes_pls_golite062009
transporter activity	2.36978240084485	bayes_pls_golite062009
ATPase activity	2.26864012421561	bayes_pls_golite062009
nucleic acid binding	2.21184938313192	bayes_pls_golite062009
transmembrane transporter activity	2.18065576425044	bayes_pls_golite062009
ATPase activity, coupled	2.07184330056272	bayes_pls_golite062009
DNA binding	1.94055378599526	bayes_pls_golite062009
transcription regulator activity	1.83584389083042	bayes_pls_golite062009
structural constituent of ribosome	1.80055405467543	bayes_pls_golite062009
helicase activity	1.70242731830224	bayes_pls_golite062009
RNA binding	1.60613159109369	bayes_pls_golite062009
ribonucleotide binding	1.53142082854758	bayes_pls_golite062009
purine nucleotide binding	1.52938239070386	bayes_pls_golite062009
purine ribonucleotide binding	1.52887308780164	bayes_pls_golite062009
nucleotide binding	1.52746968836881	bayes_pls_golite062009
ubiquitin-protein ligase activity	1.52494005252804	bayes_pls_golite062009
hydrolase activity	1.44476395406467	bayes_pls_golite062009
	1.44426272235796	bayes_pls_golite062009
RNA helicase activity	1.42791449104435	bayes_pls_golite062009
RNA-dependent ATPase activity	1.39335733374328	bayes_pls_golite062009
ATP-dependent RNA helicase activity	1.36724390116598	bayes_pls_golite062009
microfilament motor activity	1.28029120876672	bayes_pls_golite062009
small conjugating protein ligase activity	1.25258316272121	bayes_pls_golite062009
ATP binding	1.23942044024113	bayes_pls_golite062009
DNA-dependent ATPase activity	1.2155186842031	bayes_pls_golite062009
adenyl ribonucleotide binding	1.21044170258803	bayes_pls_golite062009
adenyl nucleotide binding	1.19481945569892	bayes_pls_golite062009
purine NTP-dependent helicase activity	1.19311444180077	bayes_pls_golite062009
ATP-dependent helicase activity	1.19311444180077	bayes_pls_golite062009
substrate-specific transporter activity	1.09373202836575	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
microtubule binding	0.889065347963087	bayes_pls_golite062009
structural molecule activity	0.86163779946438	bayes_pls_golite062009
cytoskeletal protein binding	0.832861637931298	bayes_pls_golite062009
tubulin binding	0.818881964708536	bayes_pls_golite062009
DNA polymerase activity	0.795745617990735	bayes_pls_golite062009
structure-specific DNA binding	0.782430219754236	bayes_pls_golite062009
DNA-directed DNA polymerase activity	0.771854032852428	bayes_pls_golite062009
acid-amino acid ligase activity	0.736988322066518	bayes_pls_golite062009
DNA helicase activity	0.72881469450553	bayes_pls_golite062009
transcription factor activity	0.697256579145077	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	0.696000326143746	bayes_pls_golite062009
transcription repressor activity	0.677612020025357	bayes_pls_golite062009
protein-DNA loading ATPase activity	0.650964453464311	bayes_pls_golite062009
hydrogen ion transporting ATP synthase activity, rotational mechanism	0.643562482062891	bayes_pls_golite062009
kinase activity	0.641541011250583	bayes_pls_golite062009
double-stranded DNA binding	0.6071273686445	bayes_pls_golite062009
ATP-dependent DNA helicase activity	0.5886142102548	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.584453361662212	bayes_pls_golite062009
DNA clamp loader activity	0.543512707823441	bayes_pls_golite062009
GTPase activity	0.437665353870877	bayes_pls_golite062009
active transmembrane transporter activity	0.434775387083003	bayes_pls_golite062009
ligase activity, forming carbon-nitrogen bonds	0.39380930211325	bayes_pls_golite062009
actin-dependent ATPase activity	0.36110509588725	bayes_pls_golite062009
protein kinase activity	0.351308564667977	bayes_pls_golite062009
primary active transmembrane transporter activity	0.294474570607914	bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity	0.282352478532746	bayes_pls_golite062009
actin binding	0.269954671912367	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	0.220608508617331	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of substances	0.219533217661421	bayes_pls_golite062009
ATPase activity, coupled to movement of substances	0.219157471322311	bayes_pls_golite062009
protein binding	0.170837278876362	bayes_pls_golite062009
ion transmembrane transporter activity	0.140475613064199	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2