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Protein Overview: SUP45

General Information:
Name(s) found: SUP45 / YBR143C [SGD]
Description(s) found:
Found 27 descriptions.
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  • Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 437 amino acids

    Gene Ontology:
    Cellular Component: cytosol [IPILink to PubMed article]
    translation release factor complex [IDALink to PubMed article]
    Biological Process: translational termination [IMPLink to PubMed article][ISSLink to PubMed article]
    cytokinesis [IGILink to PubMed article][IMPLink to PubMed article]
    Molecular Function: translation release factor activity, codon specific [IMPLink to PubMed article][ISSLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDNEVEKNIE IWKVKKLVQS LEKARGNGTS MISLVIPPKG QIPLYQKMLT DEYGTASNIK  60
   61 SRVNRLSVLS AITSTQQKLK LYNTLPKNGL VLYCGDIITE DGKEKKVTFD IEPYKPINTS 120
  121 LYLCDNKFHT EVLSELLQAD DKFGFIVMDG QGTLFGSVSG NTRTVLHKFT VDLPKKHGRG 180
  181 GQSALRFARL REEKRHNYVR KVAEVAVQNF ITNDKVNVKG LILAGSADFK TDLAKSELFD 240
  241 PRLACKVISI VDVSYGGENG FNQAIELSAE ALANVKYVQE KKLLEAYFDE ISQDTGKFCY 300
  301 GIDDTLKALD LGAVEKLIVF ENLETIRYTF KDAEDNEVIK FAEPEAKDKS FAIDKATGQE 360
  361 MDVVSEEPLI EWLAANYKNF GATLEFITDK SSEGAQFVTG FGGIGAMLRY KVNFEQLVDE 420
  421 SEDEYYDEDE GSDYDFI

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) SUP35 SUP45
    View Details Krogan NJ, et al. (2006) PLB3 RPS10B SCM3 SUP35 SUP45 TRM12 VPS3
    View Details Gavin AC, et al. (2006) SUP35 SUP45
    View Details Gavin AC, et al. (2002) ASC1 ATG9 CLU1 CRN1 ECM29 FUN12 GLT1 HCR1 IST2 KAP123 KTR5 LSG1 MEC1 MIP6 MIS1 MRPL23 NAB3 NAB6 NIP1 NOG1 NOP7 NRD1 PRT1 RIC1 RLI1 ROM2 RPA135 RPG1 SEN54 SPT5 SUA7 SUI1 SUI2 SUI3 SUP35 SUP45 TAO3 TIF34 TIF35 TIF5 USO1 UTP20 UTP6 VAC14 VPS5 YGL036W YJL017W
    View Details Ho Y, et al. (2002) AAC3 CYS4 GND1 GSP1 MLC1 MYO4 NOP12 PUF6 RLI1 RPF2 SHE3 SPT2 SRP1 SSF1 STE23 SUL2 SUP45 TIF4631 UTP22 UTP7
    View Details Qiu et al. (2008) SUP35 SUP45

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run MLP2 #24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #11 Mitotic Prep1-TiO2 enriched, new search criteria Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data SUP45 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..10]
    [276..437]    		 PSI-BLAST 2710.0 Middle domain of eukaryotic peptide chain release factor subunit 1, ERF1; C-terminal domain of eukaryotic peptide chain release factor subunit 1, ERF1; N-terminal domain of eukaryotic peptide chain release factor subunit 1, ERF1
    2 View Details [11..138] PSI-BLAST 2710.0 Middle domain of eukaryotic peptide chain release factor subunit 1, ERF1; C-terminal domain of eukaryotic peptide chain release factor subunit 1, ERF1; N-terminal domain of eukaryotic peptide chain release factor subunit 1, ERF1
    3 View Details [139..275] PSI-BLAST 2710.0 Middle domain of eukaryotic peptide chain release factor subunit 1, ERF1; C-terminal domain of eukaryotic peptide chain release factor subunit 1, ERF1; N-terminal domain of eukaryotic peptide chain release factor subunit 1, ERF1

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • translation release factor activity
    		•  11.5102558694213 bayes_pls_golite062009
  • translation termination factor activity
    		•  11.4768548464911 bayes_pls_golite062009
  • translation release factor activity, codon specific
    		•  7.66455753522311 bayes_pls_golite062009
  • structural constituent of ribosome
    		•  2.97154934968642 bayes_pls_golite062009
  • nucleic acid binding
    		•  2.70960972397471 bayes_pls_golite062009
  • structural molecule activity
    		•  2.64319832043379 bayes_pls_golite062009
  • binding
    		•  2.24516466558786 bayes_pls_golite062009
  • translation regulator activity
    		•  1.83257472130927 bayes_pls_golite062009
  • translation factor activity, nucleic acid binding
    		•  1.81472167858216 bayes_pls_golite062009
  • RNA binding
    		•  1.59103459949328 bayes_pls_golite062009
  • mRNA binding
    		•  0.607146672130391 bayes_pls_golite062009
  • protein binding
    		•  0.381324299701367 bayes_pls_golite062009
  • catalytic activity
    		•  0.264002643439785 bayes_pls_golite062009
    2 No functions predicted.
    3 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle