Bait Protein: | SEC10 |
Organism: | Saccharomyces cerevisiae |
Publication: | De Craene, J., Coleman, J., de Martin, P.E., Pypaert, M., Anderson, S., Yates, J.R. 3rd, Ferro-Novick, S., and Novick, P. (2006). Rtn1p is involved in structuring the cortical ER. Mol Biol Cell. In Press |
Comments: | No comments. |
Raw Data: |
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FILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
EXO70 | Subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), whi... | 20 | 33 | 35.8% | 0.0107 | 71294 |
[ |
CLC1 | Clathrin light chain, subunit of the major coat protein involved in intracellular protein transport ... | 6 | 12 | 32.2% | 0.0104 | 26532 |
[ |
TIM13 | Mitochondrial intermembrane space protein, forms a complex with Tim8p that delivers a subset of hydr... | 2 | 2 | 31.4% | 0.00384 | 11286 |
[ |
EXO84 | Essential protein with dual roles in spliceosome assembly and exocytosis; the exocyst complex (Sec3p... | 22 | 33 | 30.9% | 0.00884 | 85538 |
[ |
TIM11 | Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex re... | 2 | 3 | 30.2% | 0.0063 | 10875 |
[ |
TIM44 | Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers ... | 10 | 12 | 29.0% | 0.00562 | 48854 |
[ |
ATP7 | Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily c... | 6 | 6 | 27.6% | 0.00696 | 19810 |
[ |
SEC5 | Essential 107kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p,... | 21 | 31 | 25.8% | 0.00644 | 112121 |
[ |
RPP2A | Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction... | 3 | 3 | 23.6% | 0.00571 | 10746 |
[ |
SEC3 | Subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, Exo84p) which me... | 26 | 38 | 22.5% | 0.00574 | 154694 |
[ |
SEC10 | Essential 100kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p,... | 18 | 23 | 21.5% | 0.00533 | 100342 |
[ |
SEC8 | Essential 121kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p,... | 17 | 26 | 21.5% | 0.00492 | 122224 |
[ |
SEC15 | Essential 113kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p,... | 20 | 28 | 18.8% | 0.00621 | 105064 |
[ |
MRPL13 | Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation | 4 | 5 | 18.5% | 0.00367 | 31526 |
[ |
SEC6 | Essential 88kDa subunit of the exocyst complex, which mediates polarized targeting of secretory vesi... | 14 | 20 | 17.3% | 0.00501 | 93428 |
[ |
CDC19 | Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... | 5 | 5 | 16.8% | 0.00202 | 54545 |
[ |
CYT1 | Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme... | 3 | 3 | 16.5% | 0.00196 | 34055 |
[ |
PSA1 | GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from ... | 4 | 4 | 15.8% | 0.00224 | 39566 |
[ |
PDB1 | E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved mu... | 4 | 6 | 15.6% | 0.00331 | 40054 |
[ |
RTT103 | Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription terminatio... | 4 | 4 | 15.6% | 0.00197 | 46488 |
[ |
LAT1 | Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes... | 5 | 5 | 15.4% | 0.00209 | 51818 |
[ |
KRE2 | Alpha1,2-mannosyltransferase of the Golgi involved in protein mannosylation | 4 | 6 | 15.2% | 0.00274 | 51387 |
[ |
SRV2 | CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cycl... | 4 | 5 | 14.6% | 0.00192 | 57522 |
[ |
KTR6 | Probable mannosylphosphate transferase involved in the synthesis of core oligosaccharides in protein... | 3 | 5 | 13.9% | 0.00226 | 52128 |
[ |
NUP60 | Subunit of the nuclear pore complex (NPC), functions to anchor Nup2p to the NPC in a process control... | 4 | 5 | 13.7% | 0.00187 | 59039 |
[ |
PIL1 | Primary component of eisosomes, which are large immobile cell cortex structures associated with endo... | 3 | 3 | 13.3% | 0.00179 | 38349 |
[ |
RTN1 | ER membrane protein that interacts with Sey1p to maintain ER morphology; interacts with exocyst subu... | 3 | 3 | 13.2% | 0.00205 | 32916 |
[ |
SSE1 | ATPase that is a component of the heat shock protein Hsp90 chaperone complex; binds unfolded protein... | 6 | 8 | 12.1% | 0.00233 | 77367 |
[ |
VMA4 | Subunit E of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... | 3 | 3 | 11.6% | 0.0026 | 26471 |
[ |
TEF1, TEF2 | Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... | 6 | 6 | 11.6% | 0.00264 | 50033 |
[ |
TEF1, TEF2 | Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... | 6 | 6 | 11.6% | 0.00264 | 50033 |
[ |
YGL082W | Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; g... | 2 | 3 | 10.5% | 0.00159 | 43321 |
[ |
IMD3 | Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-... | 3 | 4 | 10.5% | 0.00154 | 56585 |
[ |
LSP1 | Primary component of eisosomes, which are large immobile patch structures at the cell cortex associa... | 2 | 2 | 10.3% | 0.00118 | 38071 |
[ |
GDA1 | Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golg... | 3 | 4 | 10.2% | 0.00156 | 56822 |
[ |
YBR139W | Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent prot... | 4 | 4 | 9.1% | 0.00159 | 57639 |
[ |
ACT1 | Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functio... | 2 | 3 | 9.1% | 0.00161 | 41690 |
[ |
MCR1 | Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis | 2 | 2 | 8.9% | 0.00134 | 34138 |
[ |
NCP1 | NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately reg... | 4 | 5 | 8.5% | 0.00146 | 76772 |
[ |
GUS1 | Glutamyl-tRNA synthetase (GluRS), forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; ... | 6 | 6 | 8.4% | 0.00167 | 82663 |
[ |
MNN5 | Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of th... | 3 | 3 | 7.5% | 0.00103 | 67252 |
[ |
ARC1 | Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering t... | 2 | 3 | 7.2% | 0.00161 | 42084 |
[ |
DJP1 | Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in perox... | 2 | 2 | 7.2% | 9.34E-4 | 48575 |
[ |
RVS167 | Actin-associated protein, interacts with Rvs161p to regulate actin cytoskeleton, endocytosis, and vi... | 2 | 2 | 6.8% | 8.37E-4 | 52774 |
[ |
PIB2 | Protein binding phosphatidylinositol 3-phosphate, involved in telomere-proximal repression of gene e... | 2 | 2 | 6.5% | 6.35E-4 | 70617 |
[ |
KAR2 | ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... | 3 | 4 | 6.2% | 0.00118 | 74468 |
[ |
KAR2 | ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... | 3 | 4 | 6.2% | 0.00118 | 74468 |
[ |
PDA1 | E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarbo... | 2 | 2 | 5.6% | 9.11E-4 | 48891 |
[ |
SSC1 | Hsp70 family ATPase, constituent of the import motor component of the Translocase of the Inner Mitoc... | 2 | 3 | 5.5% | 9.25E-4 | 70628 |
[ |
DED1 | ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all ye... | 2 | 2 | 5.3% | 6.68E-4 | 65553 |
[ |
HSP60 | Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides... | 2 | 2 | 5.1% | 7.05E-4 | 60752 |
[ |
HSC82 | Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, a... | 2 | 2 | 4.4% | 5.72E-4 | 80900 |
[ |
GLT1 | NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglut... | 2 | 3 | 1.9% | 2.82E-4 | 238100 |