Protein Overview: MEP3

General Information:
Name(s) found:	MEP3 / YPR138C [SGD]
Description(s) found: Found 29 descriptions. SHOW ALL	Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease [SGD]
Organism:	Saccharomyces cerevisiae
Length:	489 amino acids
Gene Ontology:
Cellular Component:	plasma membrane [ISS][TAS]
Biological Process:	ammonium transport [IMP][ISS] nitrogen utilization [IMP]
Molecular Function:	ammonium transmembrane transporter activity [IDA][IMP][ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MARGDGHLWT ETYDSSTVAF MILGAALVFF MVPGLGFLYS GLARRKSALA LIWVVIMATL  60
   61 VGILQWYFWG YSLAFSKTAT NNKFIGNLDS FGFRNVYGKI SDDSTYPELI YAIFQMMFMC 120
  121 VALSIIAGAT AERGKLFPHM VFLFVFATLV YCPITYWIWA PGGWAYQWGV LDWAGGGNIE 180
  181 ILSAVAGFVY SYFLGRRKEN LLINFRPHNV SMVTLGTSIL WFGWLLFNAA SSLSPNMRSV 240
  241 YAFMNTCLSA TTGGMTWCLL DYRSEKKWST VGLCSGIICG LVAATPSSGC ITLYGSLIQG 300
  301 IIAGVVCNFA TKIKYYLKVD DSLDLLAEHG IAGVVGLIFN ALFAADWVIG MDGTTKHKGG 360
  361 WLTHNWKQMY IQIAYIGASA GYCAVVTAII CFVLGKIPGV HLRVTEEAEA LGLDEDQIGE 420
  421 FAYDYVEVRR DYYQWGVDTD ALHTTCNGAN SASETNPTED SQNSSLSSAT VSGQNEKSNN 480
  481 PKLHHAKEA

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		CAK1 CDC34 CDC53 CDC9 CRT10 GRR1 MEP3 MET30 MMS1 POR1 RTT101 SAF1
View Details	Qiu et al. (2008)		ATO3 MEP1 MEP2 MEP3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	MEP3	Huh WK, et al. (2003)

Searching the YRC Public Image Repository, found results:

Currently showing to of results.

[View Search in the YRC Public Image Repository]

Searching the YRC Public Image Repository, found no results.

Ginzu Version Information

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

Gene Ontology predictions

Term	Confidence	Notes
ammonium transmembrane transporter activity	10.0624820335928	bayes_pls_golite062009
organic cation transmembrane transporter activity	9.07420898067257	bayes_pls_golite062009
cation transmembrane transporter activity	7.57181833547312	bayes_pls_golite062009
ion transmembrane transporter activity	7.46308808953548	bayes_pls_golite062009
methylammonium transmembrane transporter activity	7.38326654060024	bayes_pls_golite062009
inorganic cation transmembrane transporter activity	7.08971867997216	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	7.03159982829686	bayes_pls_golite062009
transporter activity	5.3870893301235	bayes_pls_golite062009
transmembrane transporter activity	5.3046132351753	bayes_pls_golite062009
substrate-specific transporter activity	5.16067985999244	bayes_pls_golite062009
high affinity secondary active ammonium transmembrane transporter activity	3.56825523857899	bayes_pls_golite062009
amine transmembrane transporter activity	2.81164277777293	bayes_pls_golite062009
active transmembrane transporter activity	1.08017669832334	bayes_pls_golite062009

Source: Reynolds et al. 2008. Manuscript submitted

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..489]	Pfam	175.69897	Ammonium Transporter Family No confident structure predictions are available.

Protein Overview: MEP3

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: