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Protein Overview: SMC3

General Information:
Name(s) found: SMC3 / YJL074C [SGD]
Description(s) found:
Found 30 descriptions.
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  • Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 1230 amino acids

    Gene Ontology:
    Cellular Component: nucleus [IDALink to PubMed article]
    nuclear cohesin complex [TASLink to PubMed article]
    Biological Process: ascospore formation [IMPLink to PubMed article]
    mitotic sister chromatid cohesion [IMPLink to PubMed article][TASLink to PubMed article]
    synaptonemal complex assembly [IMPLink to PubMed article]
    Molecular Function: ATPase activity [ISSLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MYIKRVIIKG FKTYRNETII DNFSPHQNVI IGSNGSGKSN FFAAIRFVLS DDYSNLKREE  60
   61 RQGLIHQGSG GSVMSASVEI VFHDPDHSMI LPSGVLSRGD DEVTIRRTVG LKKDDYQLND 120
  121 RNVTKGDIVR MLETAGFSMN NPYNIVPQGK IVALTNAKDK ERLQLLEDVV GAKSFEVKLK 180
  181 ASLKKMEETE QKKIQINKEM GELNSKLSEM EQERKELEKY NELERNRKIY QFTLYDRELN 240
  241 EVINQMERLD GDYNNTVYSS EQYIQELDKR EDMIDQVSKK LSSIEASLKI KNATDLQQAK 300
  301 LRESEISQKL TNVNVKIKDV QQQIESNEEQ RNLDSATLKE IKSIIEQRKQ KLSKILPRYQ 360
  361 ELTKEEAMYK LQLASLQQKQ RDLILKKGEY ARFKSKDERD TWIHSEIEEL KSSIQNLNEL 420
  421 ESQLQMDRTS LRKQYSAIDE EIEELIDSIN GPDTKGQLED FDSELIHLKQ KLSESLDTRK 480
  481 ELWRKEQKLQ TVLETLLSDV NQNQRNVNET MSRSLANGII NVKEITEKLK ISPESVFGTL 540
  541 GELIKVNDKY KTCAEVIGGN SLFHIVVDTE ETATLIMNEL YRMKGGRVTF IPLNRLSLDS 600
  601 DVKFPSNTTT QIQFTPLIKK IKYEPRFEKA VKHVFGKTIV VKDLGQGLKL AKKHKLNAIT 660
  661 LDGDRADKRG VLTGGYLDQH KRTRLESLKN LNESRSQHKK ILEELDFVRN ELNDIDTKID 720
  721 QVNGNIRKVS NDRESVLTNI EVYRTSLNTK KNEKLILEES LNAIILKLEK LNTNRTFAQE 780
  781 KLNTFENDLL QEFDSELSKE EKERLESLTK EISAAHNKLN ITSDALEGIT TTIDSLNAEL 840
  841 ESKLIPQEND LESKMSEVGD AFIFGLQDEL KELQLEKESV EKQHENAVLE LGTVQREIES 900
  901 LIAEETNNKK LLEKANNQQR LLLKKLDNFQ KSVEKTMIKK TTLVTRREEL QQRIREIGLL 960
  961 PEDALVNDFS DITSDQLLQR LNDMNTEISG LKNVNKRAFE NFKKFNERRK DLAERASELD1020
 1021 ESKDSIQDLI VKLKQQKVNA VDSTFQKVSE NFEAVFERLV PRGTAKLIIH RKNDNANDHD1080
 1081 ESIDVDMDAE SNESQNGKDS EIMYTGVSIS VSFNSKQNEQ LHVEQLSGGQ KTVCAIALIL1140
 1141 AIQMVDPASF YLFDEIDAAL DKQYRTAVAT LLKELSKNAQ FICTTFRTDM LQVADKFFRV1200
 1201 KYENKISTVI EVNREEAIGF IRGSNKFAEV

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) IRR1 MCD1 SMC1 SMC3
    View Details (MIPS) Mewes HW, et al. (2004) IRR1 MCD1 REC8 SCC2 SMC1 SMC3
    View Details Krogan NJ, et al. (2006) IRR1 MCD1 SMC1 SMC3
    View Details Gavin AC, et al. (2006) SMC1 SMC3
    View Details Gavin AC, et al. (2002) ACC1 CCT8 CLU1 CPA2 FDH1 GCN1 GCN20 GFA1 GUS1 HHF1, HHF2 ILV1 IRR1 KAP123 LYS12 MCD1 MRPL3 NOP12 PSA1 RPA135 RPB2 RPN10 RPN11 RPN12 RPN8 RPT3 RPT5 RPT6 RVB2 SAM1 SCC4 SEC27 SMC1 SMC3 TCB1 TIF1, TIF2 TSA1 TUB3 URA7 VAS1 YHB1 YOL098C
    View Details Ho Y, et al. (2002) ASF1 CDC5 COP1 GFA1 GID8 GSY1 HTA2 IPP1 IRR1 IVY1 KAP95 MCD1 MDH1 NOP13 NUG1 PAA1 RAD53 RFC4 SMC1 SMC3 SPC72 SRP1 UBR1 URA7 UTP22 YGL081W
    View Details Qiu et al. (2008) REC8 SMC3

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run CEP3 sample cbf3 from feb 2005 Sandall S, et all (2006)
    View Run ZDS1 Sample 2- cpn2 from june 2005 McCusker D, et al (2007)
    View Run ZDS1 Sample 3 - cpn + atp, from june 2005 McCusker D, et al (2007)
    View Run MLP2 #03 Alpha Factor Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #11 Mitotic Prep1-TiO2 enriched, new search criteria Keck JM, et al. (2011)
    View Run MLP2 #08 Alpha Factor Prep4-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run None Entered #17 Mitotic Prep3-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data SMC3 Sundin BA, et al. (2004)
    View Data SMC3 Sundin BA, et al. (2004)
    View Data SMC3 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..50] PSI-BLAST 2.69897 Myosin S1 fragment, N-terminal domain; Myosin S1, motor domain
    2 View Details [51..394] PSI-BLAST 22.522879 Heavy meromyosin subfragment
    3 View Details [395..451] deduced N/A No confident structure predictions are available.
    4 View Details [452..539]
    [563..596]    		 PSI-BLAST 91.154902 Smc hinge domain
    5 View Details [540..562]
    [597..600]
    [618..636]    		 PSI-BLAST 91.154902 Smc hinge domain
    6 View Details [601..617]
    [637..663]    		 PSI-BLAST 91.154902 Smc hinge domain
    7 View Details [664..730] PSI-BLAST 9.045757 Tropomyosin
    8 View Details [731..992] PSI-BLAST 9.045757 Tropomyosin
    9 View Details [993..1045] deduced N/A No confident structure predictions are available.
    10 View Details [1046..1230] PSI-BLAST 144.94696 Smc head domain

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • microtubule motor activity
    		•  4.51968578934603 bayes_pls_golite062009
  • motor activity
    		•  4.04927796049806 bayes_pls_golite062009
  • DNA-dependent ATPase activity
    		•  2.72462789867829 bayes_pls_golite062009
  • tubulin binding
    		•  2.67743463093593 bayes_pls_golite062009
  • binding
    		•  2.64537357529343 bayes_pls_golite062009
  • microtubule binding
    		•  2.59155206524122 bayes_pls_golite062009
  • cytoskeletal protein binding
    		•  2.4101693596493 bayes_pls_golite062009
  • DNA helicase activity
    		•  2.2817164193758 bayes_pls_golite062009
  • nucleoside-triphosphatase activity
    		•  2.16381244011837 bayes_pls_golite062009
  • pyrophosphatase activity
    		•  2.13284212711072 bayes_pls_golite062009
  • hydrolase activity, acting on acid anhydrides
    		•  2.11265775498161 bayes_pls_golite062009
  • hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
    		•  2.11046854230259 bayes_pls_golite062009
  • ATP-dependent DNA helicase activity
    		•  1.88455339138556 bayes_pls_golite062009
  • actin binding
    		•  1.75397031486306 bayes_pls_golite062009
  • helicase activity
    		•  1.70242731830224 bayes_pls_golite062009
  • hydrolase activity
    		•  1.66122526893602 bayes_pls_golite062009
  • ATPase activity
    		•  1.53315090215319 bayes_pls_golite062009
  • plus-end-directed microtubule motor activity
    		•  1.38366133153678 bayes_pls_golite062009
  • protein-DNA loading ATPase activity
    		•  1.29640539057136 bayes_pls_golite062009
  • structure-specific DNA binding
    		•  1.2539059700735 bayes_pls_golite062009
  • bubble DNA binding
    		•  1.24175604630882 bayes_pls_golite062009
  • microfilament motor activity
    		•  1.20923670094205 bayes_pls_golite062009
  • purine NTP-dependent helicase activity
    		•  1.19311444180077 bayes_pls_golite062009
  • ATP-dependent helicase activity
    		•  1.19311444180077 bayes_pls_golite062009
  • DNA clamp loader activity
    		•  1.12431517057397 bayes_pls_golite062009
  • double-stranded DNA binding
    		•  1.117585097627 bayes_pls_golite062009
  • nucleic acid binding
    		•  1.08697924060619 bayes_pls_golite062009
  • catalytic activity
    		•  1.00104173493322 bayes_pls_golite062009
  • actin filament binding
    		•  0.728264616959762 bayes_pls_golite062009
  • DNA binding
    		•  0.702147676354075 bayes_pls_golite062009
  • single-stranded DNA binding
    		•  0.568457189190858 bayes_pls_golite062009
  • recombinase activity
    		•  0.45851158693664 bayes_pls_golite062009
  • purine nucleotide binding
    		•  0.442941451960958 bayes_pls_golite062009
  • purine ribonucleotide binding
    		•  0.440672169804309 bayes_pls_golite062009
  • ribonucleotide binding
    		•  0.440612929099368 bayes_pls_golite062009
  • nucleotide binding
    		•  0.433111366205869 bayes_pls_golite062009
  • RNA helicase activity
    		•  0.34746349346164 bayes_pls_golite062009
  • centromeric DNA binding
    		•  0.322818640526369 bayes_pls_golite062009
  • transcription regulator activity
    		•  0.312171320541728 bayes_pls_golite062009
  • mismatched DNA binding
    		•  0.31144582840038 bayes_pls_golite062009
  • ATP-dependent RNA helicase activity
    		•  0.30832907234141 bayes_pls_golite062009
  • RNA-dependent ATPase activity
    		•  0.29532978396745 bayes_pls_golite062009
  • four-way junction helicase activity
    		•  0.26252931460003 bayes_pls_golite062009
  • exodeoxyribonuclease V activity
    		•  0.185603938981231 bayes_pls_golite062009
  • protein binding
    		•  0.170837278876362 bayes_pls_golite062009
  • 3'-5' DNA helicase activity
    		•  0.0769112340058804 bayes_pls_golite062009
  • structural constituent of cytoskeleton
    		•  0.0111338193708552 bayes_pls_golite062009
    2 No functions predicted.
    3
    Term Confidence Notes
  • binding
    		•  3.01984970042125 bayes_pls_golite062009
  • protein heterodimerization activity
    		•  1.35977617500621 bayes_pls_golite062009
  • DNA secondary structure binding
    		•  1.33504054478953 bayes_pls_golite062009
  • structure-specific DNA binding
    		•  1.32955290929194 bayes_pls_golite062009
  • double-stranded DNA binding
    		•  1.12641995595394 bayes_pls_golite062009
  • protein binding
    		•  1.08760961845332 bayes_pls_golite062009
  • protein dimerization activity
    		•  0.966666737816339 bayes_pls_golite062009
  • nucleic acid binding
    		•  0.835977072951553 bayes_pls_golite062009
  • DNA binding
    		•  0.701080369693496 bayes_pls_golite062009
  • catalytic activity
    		•  0.24846317916908 bayes_pls_golite062009
  • sequence-specific DNA binding
    		•  0.0698018163897043 bayes_pls_golite062009
    4 No functions predicted.
    5 No functions predicted.
    6 No functions predicted.
    7 No functions predicted.
    8 No functions predicted.
    9 No functions predicted.
    10 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.96

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle