Protein Overview: POL4

General Information:
Name(s) found:	POL4 / YCR014C [SGD]
Description(s) found: Found 23 descriptions. SHOW ALL	DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta [SGD]
Organism:	Saccharomyces cerevisiae
Length:	582 amino acids
Gene Ontology:
Cellular Component:	nucleus [TAS]
Biological Process:	base-excision repair, gap-filling [TAS] double-strand break repair via nonhomologous end joining [IDA] double-strand break repair [IDA]
Molecular Function:	DNA binding [IEA] DNA-directed DNA polymerase activity [IEA][TAS] nucleotidyltransferase activity [IEA] catalytic activity [IEA] metal ion binding [IEA] transferase activity [IEA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSLKGKFFAF LPNPNTSSNK FFKSILEKKG ATIVSSIQNC LQSSRKEVVI LIEDSFVDSD  60
   61 MHLTQKDIFQ REAGLNDVDE FLGKIEQSGI QCVKTSCITK WVQNDKFAFQ KDDLIKFQPS 120
  121 IIVISDNADD GQSSTDKESE ISTDVESERN DDSNNKDMIQ ASKPLKRLLQ GDKGRASLVT 180
  181 DKTKYKNNEL IIGALKRLTK KYEIEGEKFR ARSYRLAKQS MENCDFNVRS GEEAHTKLRN 240
  241 IGPSIAKKIQ VILDTGVLPG LNDSVGLEDK LKYFKNCYGI GSEIAKRWNL LNFESFCVAA 300
  301 KKDPEEFVSD WTILFGWSYY DDWLCKMSRN ECFTHLKKVQ KALRGIDPEC QVELQGSYNR 360
  361 GYSKCGDIDL LFFKPFCNDT TELAKIMETL CIKLYKDGYI HCFLQLTPNL EKLFLKRIVE 420
  421 RFRTAKIVGY GERKRWYSSE IIKKFFMGVK LSPRELEELK EMKNDEGTLL IEEEEEEETK 480
  481 LKPIDQYMSL NAKDGNYCRR LDFFCCKWDE LGAGRIHYTG SKEYNRWIRI LAAQKGFKLT 540
  541 QHGLFRNNIL LESFNERRIF ELLNLKYAEP EHRNIEWEKK TA

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		GSG1 POL4 RPN1 RPN10 RPN11 RPN12 RPN13 RPN2 RPN3 RPN5 RPN6 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 SEM1 SLX5 SPG5 SWA2 UBP6
View Details	Krogan NJ, et al. (2006)		ARI1 POL4 ROK1
View Details	Ho Y, et al. (2002)		POL4 RHO5

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	POL4	PET494	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..91]	MSA	1.015993	View MSA. No confident structure predictions are available.
2	View Details	[92..149]	deduced	N/A	No confident structure predictions are available.
3	View Details	[150..264]	PSI-BLAST	216.0103	Terminal deoxynucleotidyl transferase
4	View Details	[265..318]	PSI-BLAST	216.0103	Terminal deoxynucleotidyl transferase
5	View Details	[319..582]	PSI-BLAST	216.0103	Terminal deoxynucleotidyl transferase

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
DNA polymerase activity	1.73758028049287	bayes_pls_golite062009
DNA-directed DNA polymerase activity	1.72676695496584	bayes_pls_golite062009
catalytic activity	0.60409960326459	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.91

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2