Protein Overview: RPT1

General Information:
Name(s) found:	RPT1 / YKL145W [SGD]
Description(s) found: Found 34 descriptions. SHOW ALL	One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and the E3 ubiquitin-protein ligase Ubr1p [SGD]
Organism:	Saccharomyces cerevisiae
Length:	467 amino acids
Gene Ontology:
Cellular Component:	proteasome regulatory particle, base subcomplex [IPI]
Biological Process:	ubiquitin-dependent protein catabolic process [TAS ]
Molecular Function:	ATPase activity [IDA][TAS] endopeptidase activity [TAS ]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPPKEDWEKY KAPLEDDDKK PDDDKIVPLT EGDIQVLKSY GAAPYAAKLK QTENDLKDIE  60
   61 ARIKEKAGVK ESDTGLAPSH LWDIMGDRQR LGEEHPLQVA RCTKIIKGNG ESDETTTDNN 120
  121 NSGNSNSNSN QQSTDADEDD EDAKYVINLK QIAKFVVGLG ERVSPTDIEE GMRVGVDRSK 180
  181 YNIELPLPPR IDPSVTMMTV EEKPDVTYSD VGGCKDQIEK LREVVELPLL SPERFATLGI 240
  241 DPPKGILLYG PPGTGKTLCA RAVANRTDAT FIRVIGSELV QKYVGEGARM VRELFEMART 300
  301 KKACIIFFDE IDAVGGARFD DGAGGDNEVQ RTMLELITQL DGFDPRGNIK VMFATNRPNT 360
  361 LDPALLRPGR IDRKVEFSLP DLEGRANIFR IHSKSMSVER GIRWELISRL CPNSTGAELR 420
  421 SVCTEAGMFA IRARRKVATE KDFLKAVDKV ISGYKKFSST SRYMQYN

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ECM29 GSG1 NAS6 POL4 RPN1 RPN10 RPN11 RPN12 RPN13 RPN2 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 SEC18 SEM1 SLX5 SPG5 SWA2 TOD6 UBP6
View Details	(MIPS) Mewes HW, et al. (2004)		NAS6 RPN1 RPN10 RPN11 RPN12 RPN2 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6
View Details	Krogan NJ, et al. (2006)		CSI1 CSN9 ERV46 FPR2 HUL5 IQG1 MOT1 MPT5 NAS6 NMD3 NPT1 PCI8 PEP1 PST1 PUS9 RPN1 RPN10 RPN11 RPN12 RPN13 RPN14 RPN2 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 RRI1 RRI2 SEC18 SEM1 SKP1 SPG5 SPO77 SQT1 SWA2 TCB2 TIP41 TOD6 TOR2 TSR4 UBP6 YEN1 YKL027W YMR258C YNL022C YPR003C YPT52
View Details	Gavin AC, et al. (2006)		ECM29 RPN1 RPN10 RPN11 RPN12 RPN13 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1
View Details	Gavin AC, et al. (2002)		BLM10 CDC6 ECM29 GFA1 MLH2 NAS6 PGK1 PRE1 PRE10 PRE2 PRE3 PRE4 PRE5 PRE6 PRE7 PRE8 PRE9 PUP3 RGR1 RPN10 RPN11 RPN12 RPN13 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 SCL1 UBP6 YFL006W
View Details	Ho Y, et al. (2002)		AAC1 AAC3 ADE5,7 AHA1 ARC1 ARO1 ARP2 ATG11 ATG12 ATG17 BUD32 CAR2 CPA2 CPR6 CRM1 DIA4 EMP24 FET4 GCD11 GFA1 GRX3 GRX4 GUS1 HEF3 IDH2 IDP2 IMD2 IMD4 IPP1 KAE1 KGD1 KRE6 LHS1 MDN1 MKT1 NAS6 NPA3 PHO81 PPX1 PRB1 PTC7 REP1 REX2 RNH202 RPN1 RPN10 RPN11 RPN12 RPN13 RPN14 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 SEC18 SEC23 SGO1 STI1 TCB1 TIF1, TIF2 TMA29 TYR1 UBC12 URA7 YHR020W YHR033W YNL208W
View Details	Qiu et al. (2008)		NAS6 PRE1 PRE10 PRE2 PRE3 PRE4 PRE5 PRE6 PRE7 PRE8 PUP1 PUP2 PUP3 RPN1 RPN10 RPN11 RPN12 RPN13 RPN2 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 SCL1 UBP6

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi1 with gst from october 2005	McCusker D, et al (2007)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RPT1	ARG81	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	RPT1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..121]	PSI-BLAST	608.37734	Membrane fusion atpase p97 N-terminal domain , P97-Nn; Membrane fusion atpase p97, D1 domain; Membrane fusion atpase p97 domain 2, P97-Nc
2	View Details	[122..205]	PSI-BLAST	608.37734	Membrane fusion atpase p97 N-terminal domain , P97-Nn; Membrane fusion atpase p97, D1 domain; Membrane fusion atpase p97 domain 2, P97-Nc
3	View Details	[206..379]	PSI-BLAST	608.37734	Membrane fusion atpase p97 N-terminal domain , P97-Nn; Membrane fusion atpase p97, D1 domain; Membrane fusion atpase p97 domain 2, P97-Nc
4	View Details	[380..467]	PSI-BLAST	608.37734	Membrane fusion atpase p97 N-terminal domain , P97-Nn; Membrane fusion atpase p97, D1 domain; Membrane fusion atpase p97 domain 2, P97-Nc

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2