Protein Overview: ABP1

General Information:
Name(s) found:	ABP1 / YCR088W [SGD]
Description(s) found: Found 22 descriptions. SHOW ALL	Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization [SGD]
Organism:	Saccharomyces cerevisiae
Length:	592 amino acids
Gene Ontology:
Cellular Component:	cytoskeleton [TAS] cytoplasm [IDA] cell cortex [IDA]
Biological Process:	actin cortical patch assembly [IMP]
Molecular Function:	protein binding [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MALEPIDYTT HSREIDAEYL KIVRGSDPDT TWLIISPNAK KEYEPESTGS SFHDFLQLFD  60
   61 ETKVQYGLAR VSPPGSDVEK IIIIGWCPDS APLKTRASFA ANFAAVANNL FKGYHVQVTA 120
  121 RDEDDLDENE LLMKISNAAG ARYSIQTSSK QQGKASTPPV KKSFTPSKSP APVSKKEPVK 180
  181 TPSPAPAAKI SSRVNDNNDD DDWNEPELKE RDFDQAPLKP NQSSYKPIGK IDLQKVIAEE 240
  241 KAKEDPRLVQ KPTAAGSKID PSSDIANLKN ESKLKRDSEF NSFLGTTKPP SMTESSLKND 300
  301 DDKVIKGFRN EKSPAQLWAE RKAKQNSGNA ETKAEAPKPE VPEDEPEGEP DVKDLKSKFE 360
  361 GLAASEKEEE EMENKFAPPP KKSEPTIISP KPFSKPQEPV KAEEAEQPKT DYKKIGNPLP 420
  421 GMHIEADNEE EPEENDDDWD DDEDEAAQPP LPSRNVASGA PVQKEEPEQE EIAPSLPSRN 480
  481 SIPAPKQEEA PEQAPEEEIE EEAEEAAPQL PSRSSAAPPP PPRRATPEKK PKENPWATAE 540
  541 YDYDAAEDNE LTFVENDKII NIEFVDDDWW LGELEKDGSK GLFPSNYVSL GN

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ABP1 BSP1 CMD1 CRN1 ECM10 EFB1 HSP104 HSP26 INP52 LSP1 MLC1 NOP53 PIL1 PRK1 RHO1 RRP1 RVS161 SAC6 SLM1 TWF1 YEF3 YNL208W
View Details	(MIPS) Mewes HW, et al. (2004)		ABP1 AIP1 ARC40 ARP2 BEM1 BUD6 CAP1 CAP2 COF1 CRN1 DLD2 GLK1 LAS17 OYE2 PAN1 PFY1 RVS167 SAC6 SLA1 SLA2 SRV2 TPM1 TPM2 TWF1 VRP1
View Details	Ho Y, et al. (2002)		ABP1 ACO1 AIM41 AIM46 AKL1 APP1 ARP3 ATP4 BUD14 CAR2 COF1 COR1 CPR1 CRZ1 CYS4 DCP1 DCP2 DNM1 ECM10 EDC3 EDE1 EGD2 ENP1 FYV8 GAS1 GCN3 GPH1 HHF1, HHF2 HRR25 HSP104 HXT7 HYP2 IPP1 LOC1 LSP1 LTV1 MDH1 MDS3 MGE1 NSA1 NUG1 OYE2 PEX19 PGM2 PIL1 PIN4 PRK1 PSE1 PTC4 PUF3 RIO2 RPC19 RPM2 SAP185 SAP190 SAS10 SCP1 SEC2 SEC23 SES1 SFB3 SGM1 SIT4 SPS1 TMA19 TSR1 TUP1 VMA4 YBR225W YER138C YSC84
View Details	Qiu et al. (2008)		ABP1 ACT1 AIM21 AIP1 ARC40 ARP2 BBC1 BEM1 BNI1 BSP1 BUD6 BZZ1 CAP1 CAP2 COF1 CRN1 DLD2 END3 ENT1 ENT2 ENT3 GLK1 IQG1 LAS17 MYO3 MYO5 OYE2 PAN1 PFY1 RVS161 RVS167 SAC6 SCD5 SLA1 SLA2 SRV2 TPM1 TPM2 TWF1 VRP1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	ABP1	CLA4	2	Drees BL, et al. (2001)
View Screen	ABP1	HUA2	2	Drees BL, et al. (2001)

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	ABP1 and SAC6	Huh WK, et al. (2003)
View Data	ABP1	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..170]	PSI-BLAST	677.30103	Cofilin-like domain of actin-binding protein abp1p
2	View Details	[171..444]	ORFEUS	8.4	Fibrinogen
3	View Details	[445..533]	ORFEUS	13.76	Sec24
4	View Details	[534..592]	PSI-BLAST	291.522879	Actin binding protein ABP1

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
actin binding	7.52071741004022	bayes_pls_golite062009
cytoskeletal protein binding	6.04859625643932	bayes_pls_golite062009
actin filament binding	5.45532510538573	bayes_pls_golite062009
binding	2.27089902377783	bayes_pls_golite062009
protein binding	1.90274942416202	bayes_pls_golite062009
phosphatidylinositol-4,5-bisphosphate binding	0.72248054722423	bayes_pls_golite062009
structural constituent of cytoskeleton	0.482954032096802	bayes_pls_golite062009
actin monomer binding	0.224304003054399	bayes_pls_golite062009

No functions predicted.

Term	Confidence	Notes
actin binding	4.94949685871786	bayes_pls_golite062009
cytoskeletal protein binding	4.41722924924158	bayes_pls_golite062009
actin filament binding	3.69427077755816	bayes_pls_golite062009
binding	2.49184519104882	bayes_pls_golite062009
protein binding	2.10925438883926	bayes_pls_golite062009
structural constituent of muscle	1.44117328570973	bayes_pls_golite062009
structural molecule activity	0.423179340849406	bayes_pls_golite062009
structural constituent of cytoskeleton	0.371928447183203	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2