Abstract
Eukaryotic pre-ribosomes go through cytoplasmic maturation steps before entering translation. The nucleocytoplasmic proteins participating in these late stages of maturation are reimported to the nucleus. In this study, we describe a functional network focused on Rei1/Ybr267w, a strictly cytoplasmic pre-60S factor indirectly involved in nuclear 27S pre-ribosomal RNA processing. In the absence of Rei1, the nuclear import of at least three other pre-60S factors is impaired. The accumulation in the cytoplasm of a small complex formed by the association of Arx1 with a novel factor, Alb1/Yjl122w, inhibits the release of the putative antiassociation factor Tif6 from the premature large ribosomal subunits and its recycling to the nucleus. We propose a model in which Rei1 is a key factor for the coordinated dissociation and recycling of the last pre-60S factors before newly synthesized large ribosomal subunits enter translation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Active Transport, Cell Nucleus
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Carrier Proteins / genetics
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Carrier Proteins / metabolism
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Cell Nucleus / metabolism*
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Intermediate Filament Proteins / genetics
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Intermediate Filament Proteins / metabolism
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Membrane Transport Proteins / genetics
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Membrane Transport Proteins / metabolism
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Models, Biological
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Mutation
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Phosphoproteins / genetics
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Phosphoproteins / metabolism
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Polyribosomes / chemistry
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Polyribosomes / metabolism
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Protein Binding
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RNA-Binding Proteins / genetics
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RNA-Binding Proteins / metabolism
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RNA-Binding Proteins / physiology*
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Receptors, Cytoplasmic and Nuclear / genetics
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Receptors, Cytoplasmic and Nuclear / metabolism
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Ribosomal Proteins / genetics
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Ribosomal Proteins / metabolism
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Ribosomal Proteins / physiology*
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Ribosomes / chemistry
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Ribosomes / metabolism*
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism
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Saccharomyces cerevisiae Proteins / physiology*
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Two-Hybrid System Techniques
Substances
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Alb1 protein, S cerevisiae
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Carrier Proteins
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Intermediate Filament Proteins
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Membrane Transport Proteins
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NMD3 protein, S cerevisiae
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PSE1 protein, S cerevisiae
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Phosphoproteins
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REI1 protein, S cerevisiae
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RNA-Binding Proteins
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Receptors, Cytoplasmic and Nuclear
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Ribosomal Proteins
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Saccharomyces cerevisiae Proteins
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TIF6 protein, S cerevisiae
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ribosomal protein L24
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ribosomal protein L25