Protein Overview: STE7

General Information:
Name(s) found:	STE7 / YDL159W [SGD]
Description(s) found: Found 27 descriptions. SHOW ALL	Signal transducing MAP kinase kinase involved in pheromone response, where it phosphorylates Fus3p, and in the pseudohyphal/invasive growth pathway, through phosphorylation of Kss1p; phosphorylated by Ste11p, degraded by ubiquitin pathway [SGD]
Organism:	Saccharomyces cerevisiae
Length:	515 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA] mating projection tip [IDA]
Biological Process:	pseudohyphal growth [IMP] invasive growth in response to glucose limitation [IMP] protein amino acid phosphorylation [ISS] pheromone-dependent signal transduction involved in conjugation with cellular fusion [TAS]
Molecular Function:	MAP kinase kinase activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MFQRKTLQRR NLKGLNLNLH PDVGNNGQLQ EKTETHQGQS RIEGHVMSNI NAIQNNSNLF  60
   61 LRRGIKKKLT LDAFGDDQAI SKPNTVVIQQ PQNEPVLVLS SLSQSPCVSS SSSLSTPCII 120
  121 DAYSNNFGLS PSSTNSTPST IQGLSNIATP VENEHSISLP PLEESLSPAA ADLKDTLSGT 180
  181 SNGNYIQLQD LVQLGKIGAG NSGTVVKALH VPDSKIVAKK TIPVEQNNST IINQLVRELS 240
  241 IVKNVKPHEN IITFYGAYYN QHINNEIIIL MEYSDCGSLD KILSVYKRFV QRGTVSSKKT 300
  301 WFNELTISKI AYGVLNGLDH LYRQYKIIHR DIKPSNVLIN SKGQIKLCDF GVSKKLINSI 360
  361 ADTFVGTSTY MSPERIQGNV YSIKGDVWSL GLMIIELVTG EFPLGGHNDT PDGILDLLQR 420
  421 IVNEPSPRLP KDRIYSKEMT DFVNRCCIKN ERERSSIHEL LHHDLIMKYV SPSKDDKFRH 480
  481 WCRKIKSKIK EDKRIKREAL DRAKLEKKQS ERSTH

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		FUS3 KSS1 STE11 STE5 STE7
View Details	Ho Y, et al. (2002)		ACO1 ARP7 BCK2 BEM3 DIG1 DIG2 FET4 GFA1 GUS1 HAS1 HXT6 KSS1 MKT1 MSE1 NAP1 PHO84 PIM1 PMA1 PYC1 RGC1 RNH203 RPA135 RPN10 RVB1 SEN1 STE11 STE12 STE7 TEC1 UBI4 YDR239C YHR033W
View Details	Qiu et al. (2008)		FUS3 KSS1 STE11 STE20 STE4 STE5 STE7

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	KSS1	STE7	2	Uetz P, et al. (2000)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	STE7	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..131]	deduced	N/A	No confident structure predictions are available.
2	View Details	[132..273]	PSI-BLAST	379.9794	pak1
3	View Details	[274..515]	PSI-BLAST	379.9794	pak1

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
phosphotransferase activity, alcohol group as acceptor	5.49988294224815	bayes_pls_golite062009
protein kinase activity	5.4586557943719	bayes_pls_golite062009
kinase activity	5.3287080791198	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	4.96052051118534	bayes_pls_golite062009
cyclin-dependent protein kinase activity	4.62392503670521	bayes_pls_golite062009
protein serine/threonine kinase activity	4.46381887831481	bayes_pls_golite062009
transferase activity	3.97138602499442	bayes_pls_golite062009
protein tyrosine kinase activity	2.8153155526437	bayes_pls_golite062009
binding	2.74863538127007	bayes_pls_golite062009
transmembrane receptor activity	2.59526003830791	bayes_pls_golite062009
fibroblast growth factor receptor activity	2.58388574483759	bayes_pls_golite062009
type II transforming growth factor beta receptor binding	2.55651678344227	bayes_pls_golite062009
transmembrane receptor protein kinase activity	2.54265774272269	bayes_pls_golite062009
phosphoinositide 3-kinase binding	2.41610417530517	bayes_pls_golite062009
molecular transducer activity	2.40586892801446	bayes_pls_golite062009
signal transducer activity	2.40586892801446	bayes_pls_golite062009
receptor activity	2.39311927684508	bayes_pls_golite062009
protein binding	2.276111749106	bayes_pls_golite062009
ion transmembrane transporter activity	2.25301480460598	bayes_pls_golite062009
cation transmembrane transporter activity	2.188721764136	bayes_pls_golite062009
tubulin binding	2.18641675163967	bayes_pls_golite062009
transmembrane receptor protein tyrosine kinase activity	2.16831387024372	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	2.12618184009486	bayes_pls_golite062009
cytoskeletal protein binding	2.12615078788254	bayes_pls_golite062009
receptor signaling protein activity	2.10379226653669	bayes_pls_golite062009
ATP binding	1.97600611746091	bayes_pls_golite062009
ubiquitin-protein ligase activity	1.94085498993561	bayes_pls_golite062009
adenyl ribonucleotide binding	1.93596671856664	bayes_pls_golite062009
MAP kinase 2 activity	1.93464174962664	bayes_pls_golite062009
adenyl nucleotide binding	1.90928021591891	bayes_pls_golite062009
microtubule binding	1.88110801192607	bayes_pls_golite062009
receptor signaling protein serine/threonine kinase activity	1.81249386965144	bayes_pls_golite062009
telethonin binding	1.75733591474207	bayes_pls_golite062009
small conjugating protein ligase activity	1.74206248731815	bayes_pls_golite062009
PTB domain binding	1.69692410125184	bayes_pls_golite062009
purine ribonucleotide binding	1.65732943334212	bayes_pls_golite062009
ribonucleotide binding	1.65726906484847	bayes_pls_golite062009
metal ion transmembrane transporter activity	1.65350201428136	bayes_pls_golite062009
purine nucleotide binding	1.63763394711025	bayes_pls_golite062009
nucleotide binding	1.63195828048121	bayes_pls_golite062009
insulin receptor substrate binding	1.62657794525303	bayes_pls_golite062009
JUN kinase kinase kinase activity	1.60259443694567	bayes_pls_golite062009
transcription regulator activity	1.56748951434625	bayes_pls_golite062009
nucleic acid binding	1.46547714006072	bayes_pls_golite062009
MAP kinase kinase activity	1.42275431028856	bayes_pls_golite062009
DNA binding	1.35333135345112	bayes_pls_golite062009
actin binding	1.33479685165255	bayes_pls_golite062009
gamma-tubulin binding	1.28511591054866	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	1.28428643482914	bayes_pls_golite062009
kinase binding	1.26086833316556	bayes_pls_golite062009
acid-amino acid ligase activity	1.2266791546014	bayes_pls_golite062009
catalytic activity	1.21655087018101	bayes_pls_golite062009
actin-dependent ATPase activity	1.21636393741109	bayes_pls_golite062009
structural constituent of muscle	1.19908793854422	bayes_pls_golite062009
transcription elongation regulator activity	1.16995153203536	bayes_pls_golite062009
transcription repressor activity	1.14496167267281	bayes_pls_golite062009
histone kinase activity (H3-S10 specific)	1.14432210832726	bayes_pls_golite062009
transporter activity	1.11833481501707	bayes_pls_golite062009
transcription factor activity	1.10928254946881	bayes_pls_golite062009
protein kinase binding	1.06030596516506	bayes_pls_golite062009
transcription activator activity	0.997272622653026	bayes_pls_golite062009
transmembrane transporter activity	0.97575992765505	bayes_pls_golite062009
JUN kinase kinase kinase kinase activity	0.893952447158008	bayes_pls_golite062009
alpha-actinin binding	0.877087652539674	bayes_pls_golite062009
insulin-like growth factor receptor binding	0.872782211382796	bayes_pls_golite062009
fibroblast growth factor binding	0.870666814073948	bayes_pls_golite062009
growth factor binding	0.86076618871384	bayes_pls_golite062009
MAP kinase activity	0.81943579965027	bayes_pls_golite062009
cation channel activity	0.752655339263581	bayes_pls_golite062009
structural constituent of cytoskeleton	0.612883428421652	bayes_pls_golite062009
fibroblast growth factor 1 binding	0.537170519944039	bayes_pls_golite062009
protein serine/threonine/tyrosine kinase activity	0.48345772948903	bayes_pls_golite062009
enzyme binding	0.46284745266176	bayes_pls_golite062009
substrate-specific transporter activity	0.451090007290896	bayes_pls_golite062009
protein kinase C activity	0.41587987561486	bayes_pls_golite062009
RNA polymerase II transcription factor activity	0.401250804041384	bayes_pls_golite062009
magnesium ion binding	0.393328812780268	bayes_pls_golite062009
MAP kinase kinase kinase activity	0.37115026509016	bayes_pls_golite062009
structure-specific DNA binding	0.318153468064373	bayes_pls_golite062009
insulin receptor binding	0.266450215230107	bayes_pls_golite062009
substrate-specific channel activity	0.26579358730346	bayes_pls_golite062009
actin filament binding	0.2403700250846	bayes_pls_golite062009
protein complex binding	0.239339759110451	bayes_pls_golite062009
SH2 domain binding	0.226760162354251	bayes_pls_golite062009
transmembrane receptor protein serine/threonine kinase activity	0.20643152368029	bayes_pls_golite062009
transforming growth factor beta receptor activity	0.20643152368029	bayes_pls_golite062009
cell adhesion molecule binding	0.150740450460731	bayes_pls_golite062009
MAP kinase kinase kinase kinase activity	0.01826424351081	bayes_pls_golite062009
nitric-oxide synthase regulator activity	0.00407218246327123	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.0040394359628233	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2