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Protein Overview: SKI7

General Information:
Name(s) found: SKI7 / YOR076C [SGD]
Description(s) found:
Found 30 descriptions.
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  • Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 747 amino acids

    Gene Ontology:
    Cellular Component: cytoplasm [IDALink to PubMed article]
    exosome (RNase complex) [IDALink to PubMed article]
    Biological Process: response to exogenous dsRNA [IMPLink to PubMed article]
    mRNA catabolic process [IDALink to PubMed article]
    Molecular Function: protein binding [IDALink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSLLEQLARK RIEKSKGLLS ADQSHSTSKS ASLLERLHKN RETKDNNAET KRKDLKTLLA  60
   61 KDKVKRSDFT PNQHSVSLSL KLSALKKSNS DLEKQGKSVT LDSKENELPT KRKSPDDKLN 120
  121 LEESWKAIKE MNHYCFLKND PCINQTDDFA FTNFIIKDKK NSLSTSIPLS SQNSSFLSLK 180
  181 KHNNELLGIF VPCNLPKTTR KVAIENFNRP SPDDIIQSAQ LNAFNEKLEN LNIKSVPKAE 240
  241 KKEPINLQTP PTESIDIHSF IATHPLNLTC LFLGDTNAGK STLLGHLLYD LNEISMSSMR 300
  301 ELQKKSSNLD PSSSNSFKVI LDNTKTEREN GFSMFKKVIQ VENDLLPPSS TLTLIDTPGS 360
  361 IKYFNKETLN SILTFDPEVY VLVIDCNYDS WEKSLDGPNN QIYEILKVIS YLNKNSACKK 420
  421 HLIILLNKAD LISWDKHRLE MIQSELNYVL KENFQWTDAE FQFIPCSGLL GSNLNKTENI 480
  481 TKSKYKSEFD SINYVPEWYE GPTFFSQLYL LVEHNMNKIE TTLEEPFVGT ILQSSVLQPI 540
  541 AEINYVSLKV LINSGYIQSG QTIEIHTQYE DFHYYGIVSR MKNSKQILET NTKNNISVGL 600
  601 NPDILEVLVK IHNTEDFTKK QFHIRKGDII IHSRKTNTLS PNLPNTLKLL ALRLIKLSIQ 660
  661 THALSDPVDL GSELLLYHNL THNAVKLVKI LGTNDISINP NQSLIVEVEI IEPDFALNVI 720
  721 DSKYITNNIV LTSIDHKVIA VGRIACQ

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) CSL4 DIS3 LRP1 MPP6 MTR3 RRP4 RRP40 RRP42 RRP43 RRP45 RRP46 RRP6 SKI3 SKI6 SKI7 SRP1
    View Details Krogan NJ, et al. (2006) CSL4 DDI1 DIS3 ENT3 LRP1 MPP6 MTR3 OSM1 RRP4 RRP40 RRP42 RRP43 RRP45 RRP46 RRP6 SKI6 SKI7 YBL055C
    View Details Gavin AC, et al. (2006) CSL4 DIS3 MTR3 RRP4 RRP40 RRP42 RRP43 RRP45 RRP46 RRP6 SKI6 SKI7
    View Details Gavin AC, et al. (2002) CMD1 CSL4 CTR9 DIS3 ECM16 GCN1 LRP1 MAM33 MLC1 MTR3 MYO2 NMD5 RRP4 RRP40 RRP42 RRP43 RRP45 RRP46 RRP6 SAM1 SHE4 SKI2 SKI3 SKI6 SKI7 SRP1 UTP10 UTP20 UTP22 YIR035C

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run MLP2 #32 Asynchronous Prep-No Phosphopeptide enrichment Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

      BAIT PREY HITS PUBLICATION
    View Screen IMP4 SKI7 2 Unpublished Fields Lab Data

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data SKI7 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..70] deduced N/A No confident structure predictions are available.
    2 View Details [71..260] MSA 5.516997 View MSA. No confident structure predictions are available.
    3 View Details [261..293]
    [337..516]    		 PSI-BLAST 1110.0 Elongation factor eEF-1alpha, domain 2; Elongation factor eEF-1alpha, C-terminal domain; Elongation factor eEF-1alpha, N-terminal (G) domain
    4 View Details [294..336]
    [517..638]    		 PSI-BLAST 1110.0 Elongation factor eEF-1alpha, domain 2; Elongation factor eEF-1alpha, C-terminal domain; Elongation factor eEF-1alpha, N-terminal (G) domain
    5 View Details [639..747] PSI-BLAST 1110.0 Elongation factor eEF-1alpha, domain 2; Elongation factor eEF-1alpha, C-terminal domain; Elongation factor eEF-1alpha, N-terminal (G) domain

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2 No functions predicted.
    3
    Term Confidence Notes
  • ribonuclease activity
    		•  4.3040603813219 bayes_pls_golite062009
  • hydrolase activity
    		•  2.55157966585974 bayes_pls_golite062009
  • exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters
    		•  1.44266227866728 bayes_pls_golite062009
  • binding
    		•  1.41264401383678 bayes_pls_golite062009
  • exonuclease activity
    		•  1.15670533472751 bayes_pls_golite062009
  • nuclease activity
    		•  1.06224744208356 bayes_pls_golite062009
  • catalytic activity
    		•  1.00104173493322 bayes_pls_golite062009
  • structural constituent of ribosome
    		•  0.860102064846772 bayes_pls_golite062009
  • hydrolase activity, acting on ester bonds
    		•  0.759324158861044 bayes_pls_golite062009
  • RNA binding
    		•  0.707596564331465 bayes_pls_golite062009
  • nucleic acid binding
    		•  0.625617128641089 bayes_pls_golite062009
  • purine nucleotide binding
    		•  0.569910969126728 bayes_pls_golite062009
  • purine ribonucleotide binding
    		•  0.568840867400671 bayes_pls_golite062009
  • ribonucleotide binding
    		•  0.568779924702229 bayes_pls_golite062009
  • nucleotide binding
    		•  0.564275755355315 bayes_pls_golite062009
    		• 0.40076293158639 bayes_pls_golite062009
  • translation regulator activity
    		•  0.29096092258568 bayes_pls_golite062009
  • translation factor activity, nucleic acid binding
    		•  0.274994961137851 bayes_pls_golite062009
  • translation termination factor activity
    		•  0.269303802954244 bayes_pls_golite062009
  • translation release factor activity
    		•  0.252222008028593 bayes_pls_golite062009
  • ATP binding
    		•  0.1860481660648 bayes_pls_golite062009
  • adenyl ribonucleotide binding
    		•  0.152859582615242 bayes_pls_golite062009
  • nucleoside-triphosphatase activity
    		•  0.147579541298736 bayes_pls_golite062009
  • adenyl nucleotide binding
    		•  0.138615225841109 bayes_pls_golite062009
  • pyrophosphatase activity
    		•  0.133017653323467 bayes_pls_golite062009
  • hydrolase activity, acting on acid anhydrides
    		•  0.113504049290503 bayes_pls_golite062009
  • structural molecule activity
    		•  0.11239762372728 bayes_pls_golite062009
  • hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
    		•  0.111293884322157 bayes_pls_golite062009
  • DNA binding
    		•  0.0064778111446141 bayes_pls_golite062009
    4 No functions predicted.
    5 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle