Protein Overview: RPT3

General Information:
Name(s) found:	RPT3 / YDR394W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|927327\|gb\|AAB64836.1\| Yta2p; CAI: 0.21 [Saccharomyces cerevisiae] [NCBI NR] gi\|6320602\|ref\|NP_010682.1\| One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B; Rpt3p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S69678 26S proteasome regulatory particle chain RPT3 - yeast (Saccharomyces cerevisiae) [NCBI NR] RPT3 26S proteasome regulatory subunit [mips] One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B [SGD] RPT3, Chr IV from 1261670-1262956 [yeast_orfs2] Chr IV from 1261670-1262956 [yeastorf3.fasta] Chr IV from 1261669-1262955 [yeastorf4.fasta] RPT3 SGDID:S0002802, Chr IV from 1261669-1262955, Verified ORF [yeastflysequest.fasta] RPT3 SGDID:S0002802, Chr IV from 1261671-1262957, Verified ORF [10112005cervSGD.fasta] Rpt3p [Saccharomyces cerevisiae]gi\|927327\|gb\|AAB64836.1\| Yta2p; CAI: 0.21 [Saccharomyces cerevisiae]gi\|2133217\|pir\|\|S69678 26S proteasome regulatory particle chain RPT3 - yeast (Saccharomyces cerevisiae) [nrdb_11152004_Chlamydomonas] RPT3 SGDID:S000002802, Chr IV from 1261671-1262957, Verified ORF, "One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] RPT3 SGDID:S000002802, Chr IV from 1261673-1262959, Verified ORF, "One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B" [yeast-contam.fasta] RPT3 SGDID:S000002802, Chr IV from 1261674-1262960, Verified ORF, "One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B" [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B; Rpt3p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Yta2p; CAI: 0.21 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] 26S protease regulatory subunit 6B homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RPT3 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] RPT3 SGDID:S000002802, Chr IV from 1261681-1262967, Genome Release 64-1-1, Verified ORF, "One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B" [yeast-030211-contam.fasta] RPT3 SGDID:S000002802, Chr IV from 1261681-1262967, Genome Release 64-2-1, Verified ORF, "ATPase of the 19S regulatory particle of the 26S proteasome; one of ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B" [20141208_orf_trans.fasta] TR7544\|c0_g1_i1\|g.5562 ORF TR7544\|c0_g1_i1\|g.5562 TR7544\|c0_g1_i1\|m.5562 type:complete len:429 (+) TR7544\|c0_g1_i1:147-1433(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YDR394W SGDID:S000002802, Chr IV from 1261681-1262967, Genome Release 64-1-1, Verified ORF, "One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] ID=asmbl_105\|g.135 ORF ID=asmbl_105\|g.135 ID=asmbl_105\|m.135 type:complete len:429 (+) ID=asmbl_105:136-1422(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] 26S proteasome regulatory subunit 6B homolog OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RPT3 PE=1 SV=2 [Swanson-Carlisle-Scereplusabalone-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	428 amino acids
Gene Ontology:
Cellular Component:	proteasome regulatory particle, base subcomplex [IDA][IPI]
Biological Process:	ubiquitin-dependent protein catabolic process [TAS ]
Molecular Function:	ATPase activity [IDA][TAS] endopeptidase activity [TAS ]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MEELGIVTPV EKAVEEKPAV KSYASLLAQL NGTVNNNSAL SNVNSDIYFK LKKLEKEYEL  60
   61 LTLQEDYIKD EQRHLKRELK RAQEEVKRIQ SVPLVIGQFL EPIDQNTGIV SSTTGMSYVV 120
  121 RILSTLDREL LKPSMSVALH RHSNALVDIL PPDSDSSISV MGENEKPDVT YADVGGLDMQ 180
  181 KQEIREAVEL PLVQADLYEQ IGIDPPRGVL LYGPPGTGKT MLVKAVANST KAAFIRVNGS 240
  241 EFVHKYLGEG PRMVRDVFRL ARENAPSIIF IDEVDSIATK RFDAQTGSDR EVQRILIELL 300
  301 TQMDGFDQST NVKVIMATNR ADTLDPALLR PGRLDRKIEF PSLRDRRERR LIFGTIASKM 360
  361 SLAPEADLDS LIIRNDSLSG AVIAAIMQEA GLRAVRKNRY VILQSDLEEA YATQVKTDNT 420
  421 VDKFDFYK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ECM29 GSG1 NAS6 OSH7 POL4 PRE5 RPN1 RPN10 RPN11 RPN12 RPN13 RPN2 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 SEC18 SEM1 SLX5 SPG5 TOD6 UBP6
View Details	(MIPS) Mewes HW, et al. (2004)		NAS6 RPN1 RPN10 RPN11 RPN12 RPN2 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6
View Details	Krogan NJ, et al. (2006)		CSI1 CSN9 ERV46 FPR2 HUL5 IQG1 MOT1 MPT5 NAS6 NMD3 NPT1 PCI8 PEP1 PST1 PUS9 RPN1 RPN10 RPN11 RPN12 RPN13 RPN14 RPN2 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 RRI1 RRI2 SEC18 SEM1 SKP1 SPG5 SPO77 SQT1 SWA2 TCB2 TIP41 TOD6 TOR2 TSR4 UBP6 YEN1 YKL027W YMR258C YNL022C YPR003C YPT52
View Details	Gavin AC, et al. (2006)		RPT3 TUB3
View Details	Gavin AC, et al. (2002)		ACC1 ARO1 ASC1 BDF2 BLM10 CCT8 CDC55 CDC6 CKB1 CLU1 CPA2 DAM1 DBP9 DNA2 DYN1 ECM29 ECM5 EST3 FDH1 GCN1 GCN20 GFA1 GLY1 GUS1 HAT1 HAT2 HHF1, HHF2 HIF1 HSC82 HSM3 ICL1 IDH2 ILV1 IML1 INO4 KAP123 KAP95 KRE33 LIP2 LYS12 MAC1 MDN1 MDS3 MEC1 MET18 MIS1 MKT1 MLH2 MPH1 MRPL3 MRPS28 MSB1 MSH2 MSH6 NAS6 NIP1 NMD5 NUD1 PDA1 PDB1 PFK1 PGK1 PRE1 PRE10 PRE2 PRE3 PRE4 PRE5 PRE6 PRE7 PRE8 PRE9 PRO1 PRT1 PSA1 PSD2 PSE1 PSH1 PUP3 RAD23 RAD3 RAD34 RAD4 RAD51 RAD52 RFA1 RFA2 RGR1 RPA135 RPB2 RPC40 RPG1 RPN10 RPN11 RPN12 RPN13 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 RRP12 RSC3 RVB2 SAM1 SCL1 SEC27 SLX9 SMC3 SPA2 SRO7 SRP1 SSL1 TCB1 TEL1 TFA1 TFB1 TFB4 TFP1 TIF1, TIF2 TSA1 TSC11 TUB3 UBP6 ULP1 URA7 USO1 UTP20 VAS1 VTH2 YDJ1 YFL006W YFR006W YHB1 YMR310C YOL098C YPR077C YRA1
View Details	Ho Y, et al. (2002)		AAC1 AAC3 ACO1 ADE5,7 ADH2 AHA1 ARC1 ARO1 ARP2 ATG11 ATG12 ATG17 ATP3 ATP4 BCK1 BTN2 BUD32 CAR2 CCL1 CCT2 CCT3 CCT6 CGI121 CLU1 COP1 CPA2 CPR6 CTR1 CYR1 DBF2 DHH1 DUN1 EAP1 EGD2 FAA1 FAR1 FET4 FOL2 GAL7 GCD11 GDT1 GFA1 GND1 GPH1 GRX4 GUS1 HOM3 HOR2 HSP104 HXT6 HYP2 IDH1 IDH2 ILV5 IPP1 KAE1 KIC1 KIN2 LHS1 LSC1 LYS12 MDN1 MEH1 MET18 MKK2 MKT1 MOB1 MRE11 NAS6 NPA3 NTG1 OPY1 OYE2 PDC6 PEX19 PGM1 PGM2 PIL1 PMA1 POR1 PPX1 PRB1 PTC7 QCR2 RAD24 RAD3 RAD50 RAD59 RCN2 REP1 REX2 RFC1 RFC2 RFC3 RFC4 RFC5 RHR2 RPB3 RPN1 RPN10 RPN11 RPN12 RPN13 RPN14 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RVB1 SEC18 SEC27 SEC28 SHM2 SIS1 SKG3 SLT2 SMK1 SRP1 SSK2 STI1 TCB1 TCP1 TFP1 THI22 TIF1, TIF2 TMA29 TPS1 TYR1 UBC12 UBP15 XRS2 YCL042W YCR076C YHR020W YHR033W YKU70 YLR413W YNL208W
View Details	Qiu et al. (2008)		NAS6 PRE1 PRE10 PRE2 PRE3 PRE4 PRE5 PRE6 PRE7 PRE8 PRE9 PUP1 RPN1 RPN10 RPN11 RPN12 RPN13 RPN2 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 SCL1 UBP6

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RPT3	RPT3	2	Uetz P, et al. (2000)
View Screen	RPT3	RPT5	2	Uetz P, et al. (2000)
View Screen	RPT3	AME1	2	Uetz P, et al. (2000)
View Screen	RPT3	RPT4	2	Uetz P, et al. (2000)
View Screen	NAS6	RPT3	2	Unpublished Fields Lab Data
View Screen	RPT5	RPT3	2	Unpublished Fields Lab Data
View Screen	RPT3	EAF6	1	Unpublished Fields Lab Data
View Screen	RPT3	YNL011C	1	Unpublished Fields Lab Data
View Screen	APC5	RPT3	1	Unpublished Fields Lab Data
View Screen	RPT3	PAC2	1	Unpublished Fields Lab Data
View Screen	RPT3	BUD4	1	Unpublished Fields Lab Data
View Screen	RPT3	RPS3	1	Unpublished Fields Lab Data
View Screen	RPT3	YER137C	1	Unpublished Fields Lab Data
View Screen	RPT3	YKL147C	1	Unpublished Fields Lab Data
View Screen	RPT3	MGS1	1	Unpublished Fields Lab Data
View Screen	RPT3	DDI1	1	Unpublished Fields Lab Data
View Screen	RPT3	RPS27A	1	Unpublished Fields Lab Data
View Screen	RPT3	PCL1	1	Unpublished Fields Lab Data
View Screen	RPT3	PAB1	1	Unpublished Fields Lab Data
View Screen	RPT3	NTR2	1	Unpublished Fields Lab Data
View Screen	RPT3	CIT1	1	Unpublished Fields Lab Data
View Screen	RPT3	YFL051C	1	Unpublished Fields Lab Data
View Screen	RPT3	MRS4	1	Unpublished Fields Lab Data
View Screen	RPT3	TIR2	1	Unpublished Fields Lab Data
View Screen	RPT3	YAR023C	1	Unpublished Fields Lab Data
View Screen	RPT3	AGA2	1	Unpublished Fields Lab Data
View Screen	RPT3	RPL8B	1	Unpublished Fields Lab Data
View Screen	RPT3	TFC7	1	Unpublished Fields Lab Data
View Screen	RPT3	SWH1	1	Unpublished Fields Lab Data
View Screen	RPT3	PKP2	1	Unpublished Fields Lab Data
View Screen	RPT3	MMS22	1	Unpublished Fields Lab Data
View Screen	RPT3	CUP2	1	Unpublished Fields Lab Data
View Screen	RPT3	YLR346C	1	Unpublished Fields Lab Data
View Screen	RPT3	YCL073C	1	Unpublished Fields Lab Data
View Screen	RPT3	MDM34	1	Unpublished Fields Lab Data
View Screen	RPT3	YLR379W	1	Unpublished Fields Lab Data
View Screen	RPT3	VTS1	1	Unpublished Fields Lab Data
View Screen	RPT3	YCR101C	1	Unpublished Fields Lab Data
View Screen	RPT3	PRP31	1	Unpublished Fields Lab Data
View Screen	RPT3	NAM2	1	Unpublished Fields Lab Data
View Screen	RPT3	MEI5	1	Unpublished Fields Lab Data
View Screen	RPT3	NHP10	1	Unpublished Fields Lab Data
View Screen	RPT3	SLT2	1	Unpublished Fields Lab Data
View Screen	RPT3	HOF1	1	Unpublished Fields Lab Data
View Screen	RPT3	HSP32	1	Unpublished Fields Lab Data
View Screen	RPT3	YDL133W	1	Unpublished Fields Lab Data
View Screen	RPT3	GVP36	1	Unpublished Fields Lab Data
View Screen	RPT3	YMR147W	1	Unpublished Fields Lab Data
View Screen	RPT3	RPO26	1	Unpublished Fields Lab Data
View Screen	RPT3	PLP1	1	Unpublished Fields Lab Data
View Screen	RPT3	MSL1	1	Unpublished Fields Lab Data
View Screen	RPT3	YMR178W	1	Unpublished Fields Lab Data
View Screen	RPT3	RSC3	1	Unpublished Fields Lab Data
View Screen	RPT3	GTT1	1	Unpublished Fields Lab Data
View Screen	RPT3	MRPL24	1	Unpublished Fields Lab Data
View Screen	UTP9	RPT3	1	Unpublished Fields Lab Data
View Screen	RPT3	SRB7	1	Unpublished Fields Lab Data
View Screen	RPT3	NUP192	1	Unpublished Fields Lab Data
View Screen	RPT3	SIS1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	RPT3	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..140]	MSA	1.371994	View MSA. No confident structure predictions are available.
2	View Details	[141..343]	PSI-BLAST	557.28043	Membrane fusion atpase p97 N-terminal domain , P97-Nn; Membrane fusion atpase p97, D1 domain; Membrane fusion atpase p97 domain 2, P97-Nc
3	View Details	[344..428]	PSI-BLAST	557.28043	Membrane fusion atpase p97 N-terminal domain , P97-Nn; Membrane fusion atpase p97, D1 domain; Membrane fusion atpase p97 domain 2, P97-Nc

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2