Protein Overview: YKU70

General Information:
Name(s) found:	YKU70 / YMR284W [SGD]
Description(s) found: Found 35 descriptions. SHOW ALL	Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair [SGD]
Organism:	Saccharomyces cerevisiae
Length:	602 amino acids
Gene Ontology:
Cellular Component:	nuclear envelope [IDA] nuclear chromatin [TAS] nuclear telomeric heterochromatin [TAS]
Biological Process:	double-strand break repair via break-induced replication [IGI][IMP] double-strand break repair via homologous recombination [IMP] telomere maintenance [IMP ] chromatin assembly or disassembly [IDA] chromatin silencing [IDA][IMP] double-strand break repair via nonhomologous end joining [IDA]
Molecular Function:	RNA binding [IDA] damaged DNA binding [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MRSVTNAFGN SGELNDQVDE TGYRKFDIHE GILFCIELSE TMFKESSDLE YKSPLLEILE  60
   61 SLDELMSQLV ITRPGTAIGC YFYYCNREDA KEGIYELFPL RDINATFMKK LNDLLEDLSS 120
  121 GRISLYDYFM FQQTGSEKQV RLSVLFTFML DTFLEEIPGQ KQLSNKRVFL FTDIDKPQEA 180
  181 QDIDERARLR RLTIDLFDNK VNFATFFIGY ADKPFDNEFY SDILQLGSHT NENTGLDSEF 240
  241 DGPSTKPIDA KYIKSRILRK KEVKRIMFQC PLILDEKTNF IVGVKGYTMY THEKAGVRYK 300
  301 LVYEHEDIRQ EAYSKRKFLN PITGEDVTGK TVKVYPYGDL DINLSDSQDQ IVMEAYTQKD 360
  361 AFLKIIGFRS SSKSIHYFNN IDKSSFIVPD EAKYEGSIRT LASLLKILRK KDKIAILWGK 420
  421 LKSNSHPSLY TLSPSSVKDY NEGFYLYRVP FLDEIRKFPS LLSYDDGSEH KLDYDNMKKV 480
  481 TQSIMGYFNL RDGYNPSDFK NPLLQKHYKV LHDYLLQIET TFDENETPNT KKDRMMREDD 540
  541 SLRKLYYIRN KILESEKSED PIIQRLNKYV KIWNMFYKKF NDDNISIKEE KKPFDKKPKF 600
  601 NI

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		HAT2 POB3 RTT106 YKU70 YKU80
View Details	(MIPS) Mewes HW, et al. (2004)		YKU70 YKU80
View Details	Krogan NJ, et al. (2006)		FMP48 MUC1 YKU70 YKU80
View Details	Gavin AC, et al. (2006)		YKU70 YKU80
View Details	Gavin AC, et al. (2002)		YKU70 YKU80
View Details	Ho Y, et al. (2002)		ATP4 FRS2 HYP2 PEX19 RPT3 RVB1 YKU70
View Details	Qiu et al. (2008)		NDJ1 PCC1 YKU70

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	YKU70	Huh WK, et al. (2003)

Searching the YRC Public Image Repository, found results:

Currently showing to of results.

[View Search in the YRC Public Image Repository]

Searching the YRC Public Image Repository, found no results.

Ginzu Version Information

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

Gene Ontology predictions

Term	Confidence	Notes
double-stranded telomeric DNA binding	8.64621483095491	bayes_pls_golite062009
DNA-dependent protein kinase activity	7.33495559751688	bayes_pls_golite062009
telomeric DNA binding	5.16364366157397	bayes_pls_golite062009
transcription regulator activity	3.05720838352689	bayes_pls_golite062009
promoter binding	3.01492777192913	bayes_pls_golite062009
transcription activator activity	2.87080577911324	bayes_pls_golite062009
binding	2.75824485094584	bayes_pls_golite062009
protein binding	2.58357303743968	bayes_pls_golite062009
nucleic acid binding	2.08531850924748	bayes_pls_golite062009
DNA binding	1.91667917801679	bayes_pls_golite062009
transcription repressor activity	1.69798374416156	bayes_pls_golite062009
transcription factor activity	1.37299910766941	bayes_pls_golite062009
DNA-dependent ATPase activity	0.985753648509262	bayes_pls_golite062009
protein complex binding	0.94234534987624	bayes_pls_golite062009
sequence-specific DNA binding	0.914051610867509	bayes_pls_golite062009
single-stranded DNA binding	0.814975395718076	bayes_pls_golite062009
protein dimerization activity	0.73404855126602	bayes_pls_golite062009
DNA helicase activity	0.720743494912414	bayes_pls_golite062009
transcription corepressor activity	0.702777559747981	bayes_pls_golite062009
protein heterodimerization activity	0.64812784969409	bayes_pls_golite062009
ATP-dependent DNA helicase activity	0.506640285146188	bayes_pls_golite062009
structure-specific DNA binding	0.450760554291027	bayes_pls_golite062009
structural molecule activity	0.375125487765613	bayes_pls_golite062009
receptor binding	0.234020194580985	bayes_pls_golite062009
transcription factor binding	0.184852480703492	bayes_pls_golite062009
protein C-terminus binding	0.15516806759612	bayes_pls_golite062009
identical protein binding	0.125363466534611	bayes_pls_golite062009
collagen binding	0.113837148027681	bayes_pls_golite062009

No functions predicted.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..260] [289..313] [476..602]	PSI-BLAST	207.0	DNA binding C-terminal domain of ku70; Ku70 subunit
2	View Details	[261..288] [314..475]	PSI-BLAST	207.0	DNA binding C-terminal domain of ku70; Ku70 subunit

Protein Overview: YKU70

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: