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Protein Overview: MIS1

General Information:
Name(s) found: MIS1 / YBR084W [SGD]
Description(s) found:
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  • pir||A28174 methylenetetrahydrofolate dehydrogenase (NADP) (EC 1.5.1.5) - yeast (Saccharomyces cerevisiae) [NCBI NR]
  • gi|6319558|ref|NP_009640.1| Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF [NCBI NR]
  • gi|536348|emb|CAA85029.1| MIS1 [Saccharomyces cerevisiae] [NCBI NR]
  • gi|171953|gb|AAA34781.1| C-1-Tetrahydrofolate synthase [NCBI NR]
  • gi|115209|sp|P09440.1|C1TM_YEAST RecName: Full=C-1-tetrahydrofolate synthase, mitochondrial; Short=C1-THF synthase; Includes: RecName: Full=Methylenetetrahydrofolate dehydrogenase; Includes: RecName: Full=Methenyltetrahydrofolate cyclohydrolase; Includes: [NCBI NR]
  • C-1-tetrahydrofolate synthase, mitochondrial OS=Saccharomyces cerevisiae GN=MIS1 PE=1 SV=1 [Swiss-Prot]
  • MIS1 C1-tetrahydrofolate synthase precursor, mitochondrial [mips]
  • Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase [SGD]
  • MIS1, Chr II from 411012-413939 [yeast_orfs2]
  • Chr II from 411012-413939 [yeastorf3.fasta]
  • Chr II from 411010-413937 [yeastorf5.fasta]
  • (P09440) C-1-tetrahydrofolate synthase, mitochondrial precursor (C1-THF synthase) [Includes: Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate synthetase (EC 6.3.4.3)] [4932.FASTACCERV.txt]
  • MIS1 SGDID:S0000288, Chr II from 411011-413938, Verified ORF [yeastflysequest.fasta]
  • MIS1 SGDID:S0000288, Chr II from 411048-413975, Verified ORF [10112005cervSGD.fasta]
  • Mis1p [Saccharomyces cerevisiae]gi|536348|emb|CAA85029.1| MIS1 [Saccharomyces cerevisiae]gi|82919|pir||A28174 methylenetetrahydrofolate dehydrogenase (NADP) (EC 1.5.1.5) - yeast (Saccharomyces cerevisiae)gi|171953|gb|AAA34781.1| C-1-Tetrahydrofolate sy [nrdb_11152004_Chlamydomonas]
  • MIS1 SGDID:S000000288, Chr II from 411048-413975, Verified ORF, "Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-T [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta]
  • MIS1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • C-1-tetrahydrofolate synthase, mitochondrial precursor (C1-THF synthase) [Includes: Methylenetetrahydrofolate dehydrogenase ; Methenyltetrahydrofolate cyclohydrolase ; Formyltetrahydrofolate synthetase ] [nr-271106-contam.fasta]
  • Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase; Mis1p [Saccha [nr-271106-contam.fasta]
  • C-1-Tetrahydrofolate synthase [nr-271106-contam.fasta]
  • MIS1 SGDID:S000000288, Chr II from 411048-413975, Verified ORF, "Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase" [yeast-bovrabprots.fasta]
  • [chemostatdb.fasta]
  • C-1-tetrahydrofolate synthase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MIS1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta]
  • MIS1 SGDID:S000000288, Chr II from 411054-413981, Genome Release 64-1-1, Verified ORF, "Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase" [yeast-030211-contam.fasta]
  • MIS1 SGDID:S000000288, Chr II from 411054-413981, Genome Release 64-2-1, Verified ORF, "Mitochondrial C1-tetrahydrofolate synthase; involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase" [20141208_orf_trans.fasta]
  • YBR084W SGDID:S000000288, Chr II from 411054-413981, Genome Release 64-1-1, Verified ORF, "Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
    • Organism: Saccharomyces cerevisiae
    Length: 975 amino acids

    Gene Ontology:
    Cellular Component: mitochondrion [IDALink to PubMed articleLink to PubMed articleLink to PubMed article]
    Biological Process: ribosome biogenesis [RCALink to PubMed article]
    folic acid biosynthetic process [IDALink to PubMed article]
    anaerobic purine catabolic process [IDALink to PubMed article]
    nucleobase, nucleoside, nucleotide and nucleic acid metabolic process [IMPLink to PubMed article]
    acetate biosynthetic process from carbon monoxide [IDALink to PubMed article]
    Molecular Function: formate-tetrahydrofolate ligase activity [IDALink to PubMed article]
    methenyltetrahydrofolate cyclohydrolase activity [IDALink to PubMed article]
    methylenetetrahydrofolate dehydrogenase (NADP+) activity [IDALink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLSRLSLLSN SRAFQQARWR IYRLKVSPTV HASQYHILSG RKLAQSIREK ANDEIQAIKL  60
   61 KHPNFKPTLK IIQVGARPDS STYVRMKLKA SKDSNVDCII EKLPAEITEV ELLKKISDIN 120
  121 DDDSIHGLLI QLPLPRHLDE TTITNAVDFK KDVDGFHRYN AGELAKKGGK PYFIPCTPYG 180
  181 CMKLLEEAHV KLDGKNAVVL GRSSIVGNPI ASLLKNANAT VTVCHSHTRN IAEVVSQADI 240
  241 VIAACGIPQY VKSDWIKEGA VVIDVGINYV PDISKKSGQK LVGDVDFDSV KEKTSYITPV 300
  301 PGGVGPMTVA MLVSNVLLAA KRQFVESEKL PVIKPLPLHL ESPVPSDIDI SRAQSPKHIK 360
  361 QVAEELGIHS HELELYGHYK AKISPNIFKR LESRENGKYV LVAGITPTPL GEGKSTTTMG 420
  421 LVQALSAHLG KPSIANVRQP SLGPTLGVKG GAAGGGYAQV IPMDEFNLHL TGDIHAISAA 480
  481 NNLLAAAIDT RMFHEATQKN DSTFYKRLVP RKKGIRKFTP SMQRRLKRLD IEKEDPDALT 540
  541 PEEVKRFARL NINPDTITIR RVVDINDRML RQITIGEAAT EKGFTRTTGF DITVASELMA 600
  601 ILALSKSLHE MKERIGRMVI GADYDNKPVT VEDIGCTGAL TALLRDAIKP NLMQTLEGTP 660
  661 VMVHAGPFAN ISIGASSVIA DLMALKLVGS EKNPLNDKNI HEPGYVVTEA GFDFAMGGER 720
  721 FFDIKCRSSG LVPDAVVLVA TVRALKSHGG APNVKPGQSL PKEYTEENID FVAKGVSNLV 780
  781 KQIENIKTFG IPVVVAINRF ETDSQAEIEV IKKAALNAGA SHAVTSNHWM EGGKGAVELA 840
  841 HAVVDATKEP KNFNFLYDVN SSIEDKLTSI VQKMYGGAKI EVSPEAQKKI DTYKKQGFGN 900
  901 LPICIAKTQY SLSHDPSLKG VPRGFTFPIR DVRASIGAGY LYALAAEIQT IPGLSTYAGY 960
  961 MAVEVDDDGE IEGLF

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) ASC1 DBP2 FUN12 MIS1 RPL27A
    View Details Krogan NJ, et al. (2006) ADE3 MIS1 RPP1B TMA108
    View Details Gavin AC, et al. (2006) MIS1 PIM1
    View Details Gavin AC, et al. (2002) AAR2 ADE3 AMN1 APL2 APL4 APM1 APM2 APS1 ARD1 ARX1 ASC1 BDF2 BEM2 BMS1 BRX1 BUD14 BUD20 CBF5 CDC33 CDC55 CIC1 CKB1 CLU1 CRN1 CYR1 CYS4 DBP10 DBP2 DBP9 DCP2 DRS1 DYN1 EBP2 ECM29 ECM5 ENO1 ERB1 FPR4 FUN12 FUN30 GAR1 GCN1 GLY1 HAS1 HAT1 HAT2 HDA1 HEK2 HER1 HHF1, HHF2 HIF1 HIS3 HSM3 ICL1 IML1 INP2 IPI1 IPI3 KAP123 KIN3 KRE33 KRR1 LHP1 LIP2 LOC1 LSG1 MAC1 MAK21 MAK5 MDN1 MEC1 MIP6 MIS1 MRP7 MRPL10 MRPL23 MRT4 MSB1 MYO1 NAB3 NAB6 NAP1 NAT1 NCL1 NHP2 NIP1 NIP7 NMD3 NMD5 NOC2 NOC3 NOG1 NOG2 NOP1 NOP12 NOP15 NOP16 NOP2 NOP4 NOP56 NOP6 NOP7 NRD1 NSA1 NSA2 NUG1 PFK1 POL1 POL12 POL2 PRI1 PRI2 PRO1 PRP4 PRT1 PSD2 PSE1 PSH1 PUF6 PUS7 RAT1 RBG1 REI1 RFC1 RFC3 RFC4 RFC5 RIX1 RIX7 RLP24 RLP7 ROM2 RPA135 RPA190 RPC40 RPF1 RPF2 RPG1 RPN12 RPN3 RPN6 RPN8 RPN9 RPP2B RPT2 RPT3 RPT5 RPT6 RRP1 RRP12 RSA3 RSA4 RSC3 RTR1 SAM1 SBP1 SCP160 SDA1 SEC7 SEN54 SHM1 SKI2 SKI3 SMB1 SNP1 SNU114 SNU13 SNU66 SPB1 SPB4 SRO9 SSF1 SSZ1 STM1 STO1 SUI3 SUP35 SUP45 TAO3 TEL1 TIF1, TIF2 TIF4631 TIF4632 TIF6 TSC11 TUB3 URB1 USO1 UTP10 UTP20 UTP21 UTP22 VAC14 VPS5 VTH2 YFR006W YGL036W YGR102C YJL017W YMR310C YPL009C YPR077C YTM1 ZUO1

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run ASF1 3829 - low filter Green EM, et al (2005)
    View Run BOI1 Sample bob1 (2nd set) from october 2005 McCusker D, et al (2007)
    View Run VAM3 Sample: HX11 - both fusion and trans-SNARE complex formation take place. Hao Xu, et al. (2010)
    View Run MLP2 #32 Asynchronous Prep-No Phosphopeptide enrichment Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data MIS1 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..47]
    [159..310]    		 PSI-BLAST 844.0 Methylenetetrahydrofolate dehydrogenase/cyclohydrolase; Tetrahydrofolate dehydrogenase/cyclohydrolase
    2 View Details [48..158]
    [311..336]    		 PSI-BLAST 844.0 Methylenetetrahydrofolate dehydrogenase/cyclohydrolase; Tetrahydrofolate dehydrogenase/cyclohydrolase
    3 View Details [337..398]
    [417..707]
    [949..975]    		 PSI-BLAST 2420.0 Formyltetrahydrofolate synthetase
    4 View Details [399..416]
    [708..948]    		 PSI-BLAST 2420.0 Formyltetrahydrofolate synthetase

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2
    Term Confidence Notes
  • binding
    		•  2.64537357529343 bayes_pls_golite062009
  • nucleic acid binding
    		•  2.51683451033161 bayes_pls_golite062009
  • translation initiation factor activity
    		•  2.41232228963167 bayes_pls_golite062009
  • DNA binding
    		•  2.12258000781149 bayes_pls_golite062009
  • transcription regulator activity
    		•  2.09345198747025 bayes_pls_golite062009
  • lysine N-acetyltransferase activity
    		•  1.55478124277326 bayes_pls_golite062009
  • histone acetyltransferase activity
    		•  1.55478124277326 bayes_pls_golite062009
  • hydrolase activity
    		•  1.29060292615008 bayes_pls_golite062009
  • purine ribonucleotide binding
    		•  1.1110976601505 bayes_pls_golite062009
  • ribonucleotide binding
    		•  1.11103644113672 bayes_pls_golite062009
  • purine nucleotide binding
    		•  1.1086792521709 bayes_pls_golite062009
  • nucleotide binding
    		•  1.1060655022247 bayes_pls_golite062009
  • catalytic activity
    		•  1.00104173493322 bayes_pls_golite062009
  • transcription factor activity
    		•  0.887108427603557 bayes_pls_golite062009
  • hydrogen ion transmembrane transporter activity
    		•  0.854899277460172 bayes_pls_golite062009
  • RNA helicase activity
    		•  0.81256037545135 bayes_pls_golite062009
  • ATP binding
    		•  0.80197288940364 bayes_pls_golite062009
  • adenyl ribonucleotide binding
    		•  0.770085840885632 bayes_pls_golite062009
  • adenyl nucleotide binding
    		•  0.751575230566049 bayes_pls_golite062009
  • RNA-dependent ATPase activity
    		•  0.74918137191219 bayes_pls_golite062009
  • structural constituent of ribosome
    		•  0.738234102557832 bayes_pls_golite062009
  • monovalent inorganic cation transmembrane transporter activity
    		•  0.694120974355463 bayes_pls_golite062009
  • ATP-dependent RNA helicase activity
    		•  0.69117331267457 bayes_pls_golite062009
  • N-acyltransferase activity
    		•  0.581315571752634 bayes_pls_golite062009
  • N-acetyltransferase activity
    		•  0.487240078246257 bayes_pls_golite062009
  • kinase activity
    		•  0.431162005044036 bayes_pls_golite062009
  • ligase activity
    		•  0.417681194526243 bayes_pls_golite062009
  • inorganic cation transmembrane transporter activity
    		•  0.347619818489809 bayes_pls_golite062009
  • translation regulator activity
    		•  0.29096092258568 bayes_pls_golite062009
  • translation factor activity, nucleic acid binding
    		•  0.274994961137851 bayes_pls_golite062009
  • transferase activity, transferring phosphorus-containing groups
    		•  0.250756824614616 bayes_pls_golite062009
  • acetyltransferase activity
    		•  0.190660928077915 bayes_pls_golite062009
  • protein binding
    		•  0.170837278876362 bayes_pls_golite062009
  • nucleobase, nucleoside, nucleotide kinase activity
    		•  0.12351419478929 bayes_pls_golite062009
  • helicase activity
    		•  0.0775311459068899 bayes_pls_golite062009
  • structural molecule activity
    		•  0.0558641287316701 bayes_pls_golite062009
  • RNA binding
    		•  0.0261282189186349 bayes_pls_golite062009
    3 No functions predicted.
    4 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.73

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle