YRC Logo
PROTEIN SEARCH:
Descriptions Names[Advanced Search]

Protein Overview: PUF6

General Information:
Name(s) found: PUF6 / YDR496C [SGD]
Description(s) found:
SHOW ONLY BEST
  • gi|927766|gb|AAB64938.1| Ydr496cp; CAI: 0.25 [Saccharomyces cerevisiae] [NCBI NR]
  • gi|6320704|ref|NP_010784.1| Pumilio-homology domain protein that binds ASH1 mRNA at PUF consensus sequences in the 3' UTR and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; Puf6p [Saccharomyces cerevisiae] [NCBI NR]
  • gi|34922920|sp|Q04373.1|PUF6_YEAST RecName: Full=Pumilio homology domain family member 6 [NCBI NR]
  • pir||S69554 hypothetical protein YDR496c - yeast (Saccharomyces cerevisiae) [NCBI NR]
  • Pumilio homology domain family member 6 OS=Saccharomyces cerevisiae GN=PUF6 PE=1 SV=1 [Swiss-Prot]
  • PUF6 Member of the PUF protein family [mips]
  • Pumilio-homology domain protein that binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; also co-sediments with the 60S ribosomal subunit and is required for its biogenesis [SGD]
  • YDR496C, Chr IV from 1441436-1443406, reverse complement [yeast_orfs2]
  • Chr IV from 1441436-1443406, reverse complement [yeastorf3.fasta]
  • Chr IV from 1441419-1443389, reverse complement [yeastorf4.fasta]
  • Chr IV from 1443391-1441421, reverse complement [yeastorf5.fasta]
  • (Q04373) PUF6 protein [4932.FASTACCERV.txt]
  • PUF6 SGDID:S0002904, Chr IV from 1443391-1441421, reverse complement, Verified ORF [yeastflysequest.fasta]
  • PUF6 SGDID:S0002904, Chr IV from 1443393-1441423, reverse complement, Verified ORF [10112005cervSGD.fasta]
  • Puf6p [Saccharomyces cerevisiae]gi|927766|gb|AAB64938.1| Ydr496cp; CAI: 0.25 [Saccharomyces cerevisiae]gi|34922920|sp|Q04373|PUF6_YEAST PUF6 proteingi|2131531|pir||S69554 hypothetical protein YDR496c - yeast (Saccharomyces cerevisiae) [nrdb_11152004_Chlamydomonas]
  • PUF6 SGDID:S000002904, Chr IV from 1443393-1441423, reverse complement, Verified ORF, "Pumilio-homology domain protein that binds ASH1 mRNA at PUF consensus sequences in the 3' UTR and represses its translation, resulting in proper asymmetric localization [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta]
  • PUF6 SGDID:S000002904, Chr IV from 1443395-1441425, reverse complement, Verified ORF, "Pumilio-homology domain protein that binds ASH1 mRNA at PUF consensus sequences in the 3' UTR and represses its translation, resulting in proper asymmetric localization [yeast-contam.fasta]
  • PUF6 SGDID:S000002904, Chr IV from 1443396-1441426, reverse complement, Verified ORF, "Pumilio-homology domain protein that binds ASH1 mRNA at PUF consensus sequences in the 3' UTR and represses its translation, resulting in proper asymmetric localization [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta]
  • Protein PUF6 [nr-271106-contam.fasta]
  • Pumilio-homology domain protein that binds ASH1 mRNA at PUF consensus sequences in the 3' UTR and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; Puf6p [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • Ydr496cp; CAI: 0.25 [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • PUF6 SGDID:S000002904, Chr IV from 1443396-1441426, reverse complement, Verified ORF, "Pumilio-homology domain protein that binds ASH1 mRNA at PUF consensus sequences in the 3' UTR and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA" [yeast-bovrabprots.fasta]
  • PUF6 SGDID:S000002904, Chr IV from 1443395-1441425, reverse complement, Verified ORF, "Pumilio-homology domain protein that binds ASH1 mRNA at PUF consensus sequences in the 3' UTR and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA" [yeast-contam_custom.fasta]
  • [chemostatdb.fasta]
  • Pumilio homology domain family member 6 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PUF6 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta]
  • PUF6 SGDID:S000002904, Chr IV from 1443403-1441433, Genome Release 64-1-1, reverse complement, Verified ORF, "Pumilio-homology domain protein that binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; also co-sediments with the 60S ribosomal subunit and is required for its biogenesis" [yeast-030211-contam.fasta]
  • PUF6 SGDID:S000002904, Chr IV from 1443403-1441433, Genome Release 64-2-1, reverse complement, Verified ORF, "Pumilio-homology domain protein; binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; co-sediments with the 60S ribosomal subunit and is required for its biogenesis" [20141208_orf_trans.fasta]
  • YDR496C SGDID:S000002904, Chr IV from 1443403-1441433, Genome Release 64-1-1, reverse complement, Verified ORF, "Pumilio-homology domain protein that binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; also co-sediments with the 60S ribosomal subunit and is required for its biogenesis" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
    • Organism: Saccharomyces cerevisiae
    Length: 656 amino acids

    Gene Ontology:
    Cellular Component: nucleus [IDALink to PubMed article]
    nucleolus [IDALink to PubMed article]
    Biological Process: regulation of transcription, mating-type specific [IDALink to PubMed article][IMPLink to PubMed article]
    ribosome biogenesis [RCALink to PubMed article]
    Molecular Function: mRNA binding [IDALink to PubMed article]
    specific transcriptional repressor activity [IDALink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAPLTKKTNG KRSAKEVSHS EKKLAKKPRI SIDSSDEESE LSKKEDAVSS SSDDDDLDDL  60
   61 STSDSEAEEE ADELDISDDS EEHENENEEK EGKDKSEGGE NGNHTEQRKL LKERKMQRKS 120
  121 GTQVQQIKSV WERLRVKTPP LPKQIREKLS NEIWELSKDC ISDLVLKHDA SRIVQTLVKY 180
  181 SSKDRREQIV DALKGKFYVL ATSAYGKYLL VKLLHYGSRS SRQTIINELH GSLRKLMRHR 240
  241 EGAYVVEDLF VLYATHEQRQ QMIKEFWGSE YAVFRETHKD LTIEKVCESS IEKRNIIARN 300
  301 LIGTITASVE KGSTGFQILH AAMREYVKIA NEKEISEMIE LLHEQFAELV HTPEGSDVAC 360
  361 TLVARANAKE RKLILKALKN HAEKLIKNEY GNIVFITILN CVDDTVLVFK TFSPTVKEHL 420
  421 QEFIIDKFGR RPWLYILLGL DGKYFSPIVK NELLRYIELS KATSKKDPLQ RRHELLSKFA 480
  481 PMFLSTISKD YSSILTENLG CQFIAEVLIN DELYAQLNEK DQEKYQQVLN NILTTFKGDI 540
  541 TEEEHPIHRA FSTRLLKALI QGGKWNNKEK KVIPLKNVQG LGVPFAEKLY DEIIDSSNLL 600
  601 EWINNADSSF TIVALYETLK DQKEGKPFLK DLRGVQSKIT TDESNKGSQL LAKLLK

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) BRX1 CBF5 CIC1 DBP10 DBP7 DBP9 DRS1 EBP2 ERB1 FPR4 FUN12 HAS1 IMD3 KEM1 KRE33 MAK21 MAK5 MRT4 NIP7 NOC2 NOC3 NOG1 NOG2 NOP1 NOP12 NOP15 NOP2 NOP4 NOP56 NOP7 NSA1 NSA2 NUG1 PUF6 PWP1 RIX1 RIX7 RLP7 RPF1 RPF2 RPL15A RPL1A, RPL1B RPL25 RPL2A, RPL2B RPS4B, RPS4A RPS8B, RPS8A RRP12 SPB4 SSF1 TIF6 URB1 UTP22 YTM1
    View Details Krogan NJ, et al. (2006) JIP5 PUF6
    View Details Gavin AC, et al. (2006) BRX1 DBP10 DBP9 ERB1 FPR4 MAK21 NOC2 NOP13 NOP4 PUF6 RIX7
    View Details Gavin AC, et al. (2002) AAR2 ADA2 ADH1 ARX1 BEM2 BMS1 BRX1 BUD20 CBF5 CIC1 CLU1 CYR1 CYS4 DBP10 DBP9 DCP2 DPB2 DPB4 DRS1 EBP2 ERB1 FPR4 FUN12 FUN19 GAR1 GCN1 GCN5 HAS1 HDA1 HEM13 HER1 HHF1, HHF2 HIS3 INP2 IPI1 IPI3 ISW1 ISW2 KAP114 KAP123 KRE33 KRR1 LHP1 LOC1 LSG1 MAK21 MAK5 MDN1 MED4 MES1 MIS1 MNN4 MOT1 MRP7 MRT4 MYO1 NAP1 NGG1 NHP2 NIP1 NIP7 NMD3 NOC2 NOC3 NOG1 NOG2 NOP1 NOP12 NOP15 NOP16 NOP2 NOP4 NOP56 NOP6 NOP7 NPL6 NSA1 NSA2 NUG1 PFK2 POL2 PRP4 PRT1 PSK1 PTC1 PUF6 REI1 RET1 RIX1 RIX7 RLP24 RLP7 RPA135 RPA190 RPB2 RPC82 RPF1 RPF2 RPG1 RPN9 RPO21 RPO31 RPP2B RPT5 RPT6 RRP1 RRP12 RSA3 RSA4 RTR1 RVB2 SDA1 SEC7 SGF29 SGF73 SIN3 SIN4 SKI2 SMB1 SNU114 SNU13 SNU66 SPB1 SPB4 SPT15 SPT20 SPT7 SPT8 SRO9 SSF1 STH1 TAF1 TAF10 TAF11 TAF12 TAF13 TAF14 TAF2 TAF3 TAF4 TAF5 TAF6 TAF7 TAF8 TAF9 TBF1 TEF4 TIF4631 TIF4632 TIF6 TOP1 TUB3 URB1 UTP10 UTP20 UTP21 UTP22 VAS1 VPS1 YGL036W YGR102C YMR310C YTM1
    View Details Ho Y, et al. (2002) AAC3 AFG2 ALB1 ARX1 BUD20 CBF5 CBP2 CCM1 CDC33 CIC1 CKA1 CKA2 CKB1 CKB2 CYS4 DBP10 DBP2 DBP7 DRS1 EBP2 EGD1 EHD3 ELA1 ENP2 ERB1 EST1 FPR3 FYV4 GBP2 GDE1 GND1 GSP1 HAS1 HHF1, HHF2 HHT1, HHT2 HMO1 HOT1 HSH49 HTA1 HTB1 HTB2 IMD1 IMD2 IMD3 IMD4 ISA1 KAP95 KRE33 KRI1 KRR1 LHP1 MAM33 MGM101 MNP1 MRM1 MRPL1 MRPS16 MSS116 NMD3 NOC2 NOC3 NOG1 NOP1 NOP12 NOP13 NOP2 NOP4 NOP56 NOP58 NOP6 NOP7 NPL3 NSA2 NUG1 PDI1 PET127 POB3 POL2 POL5 PRP43 PUF6 PWP1 RIX1 RLI1 RPF2 RRP1 RRP12 RRP14 RRP5 RSM24 RSM25 SFP1 SPT16 SPT2 SRP1 SSF1 STE23 SUP45 TIF1, TIF2 TIF4631 TIF6 TIS11 TRA1 TRL1 TSR1 URB1 URB2 UTP10 UTP20 UTP22 UTP7 VMR1 VPS73 WTM2 YBL044W YER077C YER084W YPP1 YRA1 YTM1
    View Details Qiu et al. (2008) ASH1 BRX1 DBP7 DRS1 ESF1 GAR1 KRR1 NAN1 NOP15 NPL3 NSR1 PUF6 PXR1 UTP5 UTP6 UTP9

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run RPA135 No Comments Schneider, DA, et al. (2006)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data PUF6 and NOP56 Huh WK, et al. (2003)
    View Data PUF6 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..157] PSI-BLAST 131.67837 Pumilio 1
    2 View Details [158..257] PSI-BLAST 131.67837 Pumilio 1
    3 View Details [258..444] PSI-BLAST 131.67837 Pumilio 1
    4 View Details [445..656] MSA 1.032956 View MSA. No confident structure predictions are available.

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • binding
    		•  2.43298635785702 bayes_pls_golite062009
  • protein binding
    		•  1.50566694420704 bayes_pls_golite062009
  • RNA binding
    		•  1.20550543134698 bayes_pls_golite062009
  • structural molecule activity
    		•  1.20255343083458 bayes_pls_golite062009
  • nucleic acid binding
    		•  0.169755106399802 bayes_pls_golite062009
  • cytoskeletal protein binding
    		•  0.0330689772947346 bayes_pls_golite062009
    2
    Term Confidence Notes
  • binding
    		•  2.32703786067369 bayes_pls_golite062009
  • protein binding
    		•  1.3800948965226 bayes_pls_golite062009
  • structural molecule activity
    		•  1.20216103967092 bayes_pls_golite062009
  • RNA binding
    		•  1.04913960175873 bayes_pls_golite062009
  • nucleic acid binding
    		•  0.118461769548431 bayes_pls_golite062009
    3 No functions predicted.
    4 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle