Protein Overview: RPT5

General Information:
Name(s) found:	RPT5 / YOR117W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6324691\|ref\|NP_014760.1\| One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; Rpt5p [Saccharomyces cer [NCBI NR] pir\|\|S46605 26S proteasome regulatory particle chain RPT5 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|464859\|sp\|P33297.3\|PRS6A_YEAST RecName: Full=26S protease regulatory subunit 6A; AltName: Full=TAT-binding protein homolog 1; Short=TBP-1 [NCBI NR] gi\|313878\|emb\|CAA51971.1\| YTA1 [Saccharomyces cerevisiae] [NCBI NR] gi\|1420311\|emb\|CAA99315.1\| YTA1 [Saccharomyces cerevisiae] [NCBI NR] gi\|1164962\|emb\|CAA64037.1\| YOR3258w [Saccharomyces cerevisiae] [NCBI NR] gi\|1050819\|emb\|CAA62114.1\| ORF O3258 [Saccharomyces cerevisiae] [NCBI NR] (P33297) 26S protease regulatory subunit 6A (TAT-binding protein homolog 1) (TBP-1) [Swiss-Prot] 26S protease regulatory subunit 6A OS=Saccharomyces cerevisiae GN=RPT5 PE=1 SV=3 [Swiss-Prot] RPT5 26S proteasome regulatory subunit [mips] One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription [SGD] Chr XV from 545029-546333 [hmr_yeast5_DM3-1_pombe.fasta] Chr XV from 545028-546332 [yeastorf3.fasta] RPT5, Chr XV from 545028-546332 [yeast_orfs2] RPT5 SGDID:S0005643, Chr XV from 545029-546333, Verified ORF [yeastflysequest.fasta] Probable 26S protease subunit and member of the CDC48/PAS1/SEC18 family of ATPases; 26S protease regulatory subunit [Saccharomyces cerevisiae]gi\|1420311\|emb\|CAA99315.1\| YTA1 [Saccharomyces cerevisiae]gi\|1164962\|emb\|CAA64037.1\| YOR3258w [Saccharomyces ce [nrdb_11152004_Chlamydomonas] RPT5 SGDID:S000005643, Chr XV from 545029-546333, Verified ORF, "One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of tr [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] RPT5 SGDID:S000005643, Chr XV from 545030-546334, Verified ORF, "One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of tr [yeast-contam.fasta] One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; Rpt5p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] ORF O3258 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] YOR3258w [Saccharomyces cerevisiae] [nr-271106-contam.fasta] YTA1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] 26S protease regulatory subunit 6A (TAT-binding protein homolog 1) (TBP-1) [nr-271106-contam.fasta] RPT5 SGDID:S000005643, Chr XV from 545030-546334, Verified ORF, "One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription" [yeast-bovrabprots.fasta] [chemostatdb.fasta] 26S protease regulatory subunit 6A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RPT5 PE=1 SV=3 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] RPT5 SGDID:S000005643, Chr XV from 545029-546333, Genome Release 64-1-1, Verified ORF, "One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1" [yeast-030211-contam.fasta] RPT5 SGDID:S000005643, Chr XV from 545029-546333, Genome Release 64-2-1, Verified ORF, "ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1" [20141208_orf_trans.fasta] TR5675\|c0_g1_i1\|g.3901 ORF TR5675\|c0_g1_i1\|g.3901 TR5675\|c0_g1_i1\|m.3901 type:complete len:435 (+) TR5675\|c0_g1_i1:95-1399(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR7620\|c0_g1_i1\|g.5610 ORF TR7620\|c0_g1_i1\|g.5610 TR7620\|c0_g1_i1\|m.5610 type:complete len:435 (+) TR7620\|c0_g1_i1:95-1399(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YOR117W SGDID:S000005643, Chr XV from 545029-546333, Genome Release 64-1-1, Verified ORF, "One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_2296\|g.1730 ORF asmbl_2296\|g.1730 asmbl_2296\|m.1730 type:complete len:435 (+) asmbl_2296:135-1439(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] asmbl_2408\|g.1808 ORF asmbl_2408\|g.1808 asmbl_2408\|m.1808 type:complete len:435 (+) asmbl_2408:94-1398(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_2300\|g.1600 ORF ID=asmbl_2300\|g.1600 ID=asmbl_2300\|m.1600 type:complete len:435 (+) ID=asmbl_2300:135-1439(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_2411\|g.1591 ORF ID=asmbl_2411\|g.1591 ID=asmbl_2411\|m.1591 type:complete len:435 (+) ID=asmbl_2411:94-1398(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta] 26S proteasome regulatory subunit 6A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RPT5 PE=1 SV=3 [Swanson-Carlisle-Scereplusabalone-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	434 amino acids
Gene Ontology:
Cellular Component:	proteasome regulatory particle, base subcomplex [IDA][IPI]
Biological Process:	ubiquitin-dependent protein catabolic process [TAS ]
Molecular Function:	ATPase activity [IDA][TAS] endopeptidase activity [TAS ]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MATLEELDAQ TLPGDDELDQ EILNLSTQEL QTRAKLLDNE IRIFRSELQR LSHENNVMLE  60
   61 KIKDNKEKIK NNRQLPYLVA NVVEVMDMNE IEDKENSEST TQGGNVNLDN TAVGKAAVVK 120
  121 TSSRQTVFLP MVGLVDPDKL KPNDLVGVNK DSYLILDTLP SEFDSRVKAM EVDEKPTETY 180
  181 SDVGGLDKQI EELVEAIVLP MKRADKFKDM GIRAPKGALM YGPPGTGKTL LARACAAQTN 240
  241 ATFLKLAAPQ LVQMYIGEGA KLVRDAFALA KEKAPTIIFI DELDAIGTKR FDSEKSGDRE 300
  301 VQRTMLELLN QLDGFSSDDR VKVLAATNRV DVLDPALLRS GRLDRKIEFP LPSEDSRAQI 360
  361 LQIHSRKMTT DDDINWQELA RSTDEFNGAQ LKAVTVEAGM IALRNGQSSV KHEDFVEGIS 420
  421 EVQARKSKSV SFYA

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ECM29 GSG1 NAS6 POL4 RPN1 RPN10 RPN11 RPN12 RPN13 RPN14 RPN2 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 SEC18 SEM1 SLX5 SPG5 SWA2 UBP6 URA3 VPS45
View Details	(MIPS) Mewes HW, et al. (2004)		NAS6 RPN1 RPN10 RPN11 RPN12 RPN2 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6
View Details	Krogan NJ, et al. (2006)		CSI1 CSN9 ERV46 FPR2 HUL5 IQG1 MOT1 MPT5 NAS6 NMD3 NPT1 PCI8 PEP1 PST1 PUS9 RPN1 RPN10 RPN11 RPN12 RPN13 RPN14 RPN2 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 RRI1 RRI2 SEC18 SEM1 SKP1 SPG5 SPO77 SQT1 SWA2 TCB2 TIP41 TOD6 TOR2 TSR4 UBP6 YEN1 YKL027W YMR258C YNL022C YPR003C YPT52
View Details	Gavin AC, et al. (2002)		ACC1 ADA2 ADH1 ATP2 BDF2 BLM10 CCT8 CDC55 CDC6 CKB1 CLU1 CPA2 DAM1 DBP9 DYN1 ECM29 ECM5 EST3 FDH1 FUN19 GCN1 GCN20 GCN5 GFA1 GLY1 GUS1 HAT1 HAT2 HEM13 HHF1, HHF2 HIF1 HSM3 ICL1 ILV1 IML1 INO4 ISW1 KAP114 KAP123 KAP95 KRE33 LIP2 LYS12 MAC1 MDN1 MDS3 MED4 MES1 MIS1 MLH2 MNN4 MOT1 MRPL3 MSB1 MYO1 NAS6 NGG1 NIP1 NMD5 NOP1 NUD1 PDA1 PDB1 PDC1 PFK1 PFK2 PGK1 PRE1 PRE10 PRE2 PRE3 PRE4 PRE5 PRE6 PRE7 PRE8 PRE9 PRO1 PRT1 PSA1 PSD2 PSE1 PSH1 PSK1 PTC1 PUF6 PUP3 RGR1 RPA135 RPB2 RPC40 RPG1 RPN10 RPN11 RPN12 RPN13 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPO21 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 RRP12 RSC3 RVB2 SAM1 SCL1 SEC27 SGF29 SGF73 SIN3 SIN4 SLX9 SMC3 SPA2 SPT15 SPT20 SPT7 SPT8 SRP1 TAF1 TAF10 TAF11 TAF12 TAF13 TAF14 TAF2 TAF3 TAF4 TAF5 TAF6 TAF7 TAF8 TAF9 TBF1 TCB1 TEF4 TEL1 TFP1 TIF1, TIF2 TSA1 TSC11 TUB3 UBP6 ULP1 URA7 USO1 UTP20 VAS1 VPS1 VTH2 YEF3 YFL006W YFR006W YMR310C YNL313C YOL098C YPR077C YRA1
View Details	Ho Y, et al. (2002)		ARP2 CPR6 CYR1 DBF2 FAA1 GPH1 IDH2 LHS1 MDN1 MKT1 MOB1 NAS6 NPA3 RPN1 RPN10 RPN11 RPN12 RPN13 RPN14 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 SEC27 STI1 TFP1 TPS1 UBC12
View Details	Qiu et al. (2008)		NAS6 PRE1 PRE10 PRE2 PRE4 PRE5 PRE6 PRE7 PRE8 PRE9 PUP3 RPN1 RPN10 RPN11 RPN12 RPN13 RPN2 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 SCL1 UBP6

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	HIR2	No Comments	Green EM, et al (2005)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)
View Run	BOI1	Sample bob1 (2nd set) from october 2005	McCusker D, et al (2007)
View Run	MLP2	#24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RPT3	RPT5	2	Uetz P, et al. (2000)
View Screen	NAS2	RPT5	2	Unpublished Fields Lab Data
View Screen	RPT5	RPT3	2	Unpublished Fields Lab Data
View Screen	RPT5	MPC54	2	Unpublished Fields Lab Data
View Screen	RPT5	RPT4	2	Unpublished Fields Lab Data
View Screen	VAM7	RPT5	1	Unpublished Fields Lab Data
View Screen	LSM8	RPT5	1	Unpublished Fields Lab Data
View Screen	CDC13	RPT5	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	RPT5	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..158]	MSA	1.442997	View MSA. No confident structure predictions are available.
2	View Details	[159..351]	PSI-BLAST	559.10721	Membrane fusion atpase p97 N-terminal domain , P97-Nn; Membrane fusion atpase p97, D1 domain; Membrane fusion atpase p97 domain 2, P97-Nc
3	View Details	[352..434]	PSI-BLAST	559.10721	Membrane fusion atpase p97 N-terminal domain , P97-Nn; Membrane fusion atpase p97, D1 domain; Membrane fusion atpase p97 domain 2, P97-Nc

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2