Protein Overview: GLC7

General Information:
Name(s) found:	GLC7 / YER133W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6320980\|ref\|NP_011059.1\| Catalytic subunit of type 1 serine/threonine protein phosphatase, involved in many processes including glycogen metabolism, sporulation, and mitosis; interacts with multiple regulatory subunits; predominantly isolated with Sds2 [NCBI NR] gi\|603372\|gb\|AAC03231.1\| Glc7p: protein phosphatase type 1 [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S32595 phosphoprotein phosphatase (EC 3.1.3.16) 1 catalytic chain - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|417520\|sp\|P32598.1\|PP12_YEAST RecName: Full=Serine/threonine-protein phosphatase PP1-2 [NCBI NR] gi\|172219\|gb\|AAB59322.1\| protein phosphatase-1 [Saccharomyces cerevisiae] [NCBI NR] Serine/threonine-protein phosphatase PP1-2 OS=Saccharomyces cerevisiae GN=GLC7 PE=1 SV=1 [Swiss-Prot] GLC7 Ser/thr phosphoprotein phosphatase 1, catalytic chain [mips] Type 1 serine/threonine protein phosphatase catalytic subunit, involved in many processes (eg: glycogen metabolism, sporulation, mitosis); accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits [SGD] GLC7, Chr V from 432491-432667,433193-433954 [yeast_orfs2] Chr V from 432491-432667,433193-433954 [yeastorf3.fasta] (P32598) Serine/threonine protein phosphatase PP1-2 (EC 3.1.3.16) [4932.FASTACCERV.txt] GLC7 SGDID:S0000935, Chr V from 432491-432667,433193-433954, Verified ORF [yeastflysequest.fasta] Catalytic subunit of type 1 serine/threonine protein phosphatase, involved in many processes including glycogen metabolism, sporulation, and mitosis; interacts with multiple regulatory subunits; predominantly isolated with Sds22p [Saccharomyces cerevisiae [nrdb_11152004_Chlamydomonas] GLC7 SGDID:S000000935, Chr V from 432491-432667,433193-433954, Verified ORF, "Catalytic subunit of type 1 serine/threonine protein phosphatase, involved in many processes including glycogen metabolism, sporulation, and mitosis; interacts with multiple reg [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] GLC7 SGDID:S000000935, Chr V from 432491-432667,433193-433954, Verified ORF, "Type 1 serine/threonine protein phosphatase catalytic subunit, involved in many processes (eg: glycogen metabolism, sporulation, mitosis); accumulates at mating projections by i [yeast-200209-contam.fasta] protein phosphatase-1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Serine/threonine-protein phosphatase PP1-2 [nr-271106-contam.fasta] Catalytic subunit of type 1 serine/threonine protein phosphatase, involved in many processes including glycogen metabolism, sporulation, and mitosis; interacts with multiple regulatory subunits; predominantly isolated with Sds22p; Glc7p [Saccharomyces cer [nr-271106-contam.fasta] Glc7p: protein phosphatase type 1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] GLC7 SGDID:S000000935, Chr V from 432491-432667,433193-433954, Verified ORF, "Type 1 serine/threonine protein phosphatase catalytic subunit, involved in many processes (eg: glycogen metabolism, sporulation, mitosis); accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits" [yeast-bovrabprots.fasta] GLC7 SGDID:S000000935, Chr V from 432491-432667,433193-433954, Verified ORF, "Catalytic subunit of type 1 serine/threonine protein phosphatase, involved in many processes including glycogen metabolism, sporulation, and mitosis; interacts with multiple regulatory subunits; predominantly isolated with Sds22p" [yeast-contam_custom.fasta] [chemostatdb.fasta] Serine/threonine-protein phosphatase PP1-2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GLC7 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] GLC7 SGDID:S000000935, Chr V from 432495-432671,433197-433958, Genome Release 64-1-1, Verified ORF, "Type 1 serine/threonine protein phosphatase catalytic subunit, involved in many processes (eg: glycogen metabolism, sporulation, mitosis); accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits" [yeast-030211-contam.fasta] GLC7 SGDID:S000000935, Chr V from 432495-432671,433197-433958, Genome Release 64-2-1, Verified ORF, "Type 1 serine/threonine protein phosphatase catalytic subunit; cleavage and polyadenylation factor (CPF) component; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest" [20141208_orf_trans.fasta] TR644\|c0_g1_i1\|g.418 ORF TR644\|c0_g1_i1\|g.418 TR644\|c0_g1_i1\|m.418 type:complete len:313 (+) TR644\|c0_g1_i1:706-1644(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR291\|c0_g1_i1\|g.206 ORF TR291\|c0_g1_i1\|g.206 TR291\|c0_g1_i1\|m.206 type:complete len:313 (+) TR291\|c0_g1_i1:145-1083(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YER133W SGDID:S000000935, Chr V from 432495-432671,433197-433958, Genome Release 64-1-1, Verified ORF, "Type 1 serine/threonine protein phosphatase catalytic subunit, involved in many processes (eg: glycogen metabolism, sporulation, mitosis); accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_7642\|g.5516 ORF asmbl_7642\|g.5516 asmbl_7642\|m.5516 type:complete len:313 (+) asmbl_7642:145-1083(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_7620\|g.4825 ORF ID=asmbl_7620\|g.4825 ID=asmbl_7620\|m.4825 type:complete len:313 (+) ID=asmbl_7620:145-1083(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_7322\|g.4459 ORF ID=asmbl_7322\|g.4459 ID=asmbl_7322\|m.4459 type:complete len:313 (+) ID=asmbl_7322:88-1026(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	312 amino acids
Gene Ontology:
Cellular Component:	protein phosphatase type 1 complex [IDA] mRNA cleavage and polyadenylation specificity factor complex [IPI] cellular bud neck [IDA] spindle pole body [IDA] nuclear outer membrane [IMP] nucleolus [IDA] mating projection base [IDA]
Biological Process:	mitotic cell cycle spindle assembly checkpoint [IMP] cell morphogenesis during vegetative growth [IGI] protein amino acid dephosphorylation [IDA] chromosome segregation [IMP] cell budding [IMP] cellular ion homeostasis [IMP] meiosis [IPI] regulation of carbohydrate metabolic process [IGI][IPI] ascospore formation [IMP] response to heat [IMP][IPI] termination of RNA polymerase II transcription, poly(A)-coupled [IPI] glycogen metabolic process [IMP] termination of RNA polymerase II transcription, poly(A)-independent [IPI] histone dephosphorylation [IMP]
Molecular Function:	protein serine/threonine phosphatase activity [IDA] phosphoprotein phosphatase activity [IEA] RNA binding [RCA] metal ion binding [IEA] hydrolase activity [IEA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDSQPVDVDN IIDRLLEVRG SKPGQQVDLE ENEIRYLCSK ARSIFIKQPI LLELEAPIKI  60
   61 CGDIHGQYYD LLRLFEYGGF PPESNYLFLG DYVDRGKQSL ETICLLLAYK IKYPENFFIL 120
  121 RGNHECASIN RIYGFYDECK RRYNIKLWKT FTDCFNCLPI AAIIDEKIFC MHGGLSPDLN 180
  181 SMEQIRRVMR PTDIPDVGLL CDLLWSDPDK DIVGWSENDR GVSFTFGPDV VNRFLQKQDM 240
  241 ELICRAHQVV EDGYEFFSKR QLVTLFSAPN YCGEFDNAGA MMSVDESLLC SFQILKPAQK 300
  301 SLPRQAGGRK KK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ADE16 CFT1 CFT2 FIP1 FPR3 GLC7 GLC8 MPE1 PAP1 PFS2 PTA1 PTI1 REF2 REG1 RNA14 SDS22 SSU72 SWD2 SYC1 YSH1 YTH1
View Details	Hazbun TR, et al. (2003)		GLC7 SDS22 YPI1
View Details	(MIPS) Mewes HW, et al. (2004)		GAC1 GIP2 GLC7 GLC8 REG1 SDS22
View Details	Krogan NJ, et al. (2006)		BNI4 BUD14 GIP3 GLC7 GLC8 HER1 PPZ1 PUF3 SDS22 SOL1 SOL2 YLR257W YPI1
View Details	Gavin AC, et al. (2006)		ADE16 GLC7 SOL2
View Details	Gavin AC, et al. (2002)		ACT1 ADE4 AIM31 BFR2 BMS1 BUD21 CBF5 CFT1 CFT2 CKA1 CLP1 DBP8 DIP2 ECM16 ECM29 EMG1 ENO2 ENP1 ENP2 ERO1 ESF1 ESF2 FIP1 GCN1 GLC7 HAS1 HCA4 HHF1, HHF2 HSL1 HTA1 HTA2 IMP3 IMP4 KRE33 KRR1 MPE1 MPP10 MVD1 NAN1 NOC4 NOP1 NOP12 NOP14 NOP56 NOP58 NOP6 PAP1 PCF11 PFK1 PFS2 PRE6 PRE9 PRP43 PTA1 PTI1 PWP2 REF2 RML2 RNA14 RNA15 ROK1 RPP2B RRP9 RSA3 RTG2 SCL1 SDS22 SEC13 SEC21 SEC31 SOF1 SRO9 SSA3 SSU72 SYC1 TAF6 TIF1, TIF2 TIF4632 TYE7 UTP10 UTP11 UTP13 UTP15 UTP18 UTP20 UTP21 UTP22 UTP30 UTP4 UTP6 UTP7 UTP8 UTP9 VID24 VMR1 VPS53 YCL046W YSH1 YTH1
View Details	Ho Y, et al. (2002)		ADH4 ADK1 ATP3 ATP5 ATP7 CDC14 CDC25 CFT1 CIC1 CKA2 CLU1 DCP2 DPM1 EDC3 EDE1 ENP1 ERB1 FIN1 FIP1 FPR3 FPR4 FUR1 GDB1 GIP2 GLC7 GLC8 GPH1 GSY1 GSY2 HEF3 HER1 HMS1 HRR25 HXT6 KEL1 KOG1 MCR1 MDH1 MDS3 MGE1 MHP1 NET1 NOP6 NOP7 NSA1 NSR1 PDC5 PHO85 PMA1 PMA2 PPZ2 REG1 RPM2 RRP17 RSE1 RVB1 SCD5 SDS22 SEC23 SEN1 SFB3 SGM1 SIT4 SNF4 SPB1 SPE3 SSZ1 SUI2 SUI3 TGL1 TPS1 TSA2 TSR1 VAS1 YGR130C YPI1 YTM1
View Details	Qiu et al. (2008)		GAC1 GLC7 PPH22 REF2 RTS1 RTS3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	YPI1	No Comments	Hazbun TR, et al. (2003)
View Run	YPI1	No Comments	Hazbun TR, et al. (2003)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	MLP2	#20 Asynchronous Prep1-TiO2 Phosphopeptide enrichment, PartB	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	GLC7	BNI4	2	Uetz P, et al. (2000)
View Screen	GLC7	NFI1	2	Unpublished Fields Lab Data
View Screen	GLC7	GIP1	2	Uetz P, et al. (2000)
View Screen	GLC7	GEP4	2	Uetz P, et al. (2000)
View Screen	GLC7	BDF2	2	Uetz P, et al. (2000)
View Screen	GLC7	PAN1	2	Uetz P, et al. (2000)
View Screen	GLC7	SFG1	2	Uetz P, et al. (2000)
View Screen	GLC7	MHP1	2	Uetz P, et al. (2000)
View Screen	GLC7	SCD5	2	Uetz P, et al. (2000)
View Screen	GLC7	FIN1	2	Uetz P, et al. (2000)
View Screen	GLC7	REF2	2	Uetz P, et al. (2000)
View Screen	GLC7	RRP17	2	Uetz P, et al. (2000)
View Screen	YPI1	GLC7	2	Hazbun TR, et al. (2003)
View Screen	GLC7	AVT6	1	Unpublished Fields Lab Data
View Screen	GLC7	RRB1	1	Unpublished Fields Lab Data
View Screen	GLC7	YER158C	1	Unpublished Fields Lab Data
View Screen	GLC7	SPG5	1	Unpublished Fields Lab Data
View Screen	GLC7	NUP49	1	Unpublished Fields Lab Data
View Screen	CDC53	GLC7	1	Unpublished Fields Lab Data
View Screen	GLC7	VID30	1	Unpublished Fields Lab Data
View Screen	GLC7	SOL1	1	Unpublished Fields Lab Data
View Screen	SWI5	GLC7	1	Unpublished Fields Lab Data
View Screen	GLC7	YGR228W	1	Unpublished Fields Lab Data
View Screen	GLC7	YPR137C-A, YAR010C	1	Unpublished Fields Lab Data
View Screen	GLC7	APM2	1	Unpublished Fields Lab Data
View Screen	GLC7	PET123	1	Unpublished Fields Lab Data
View Screen	GLC7	MPC54	1	Unpublished Fields Lab Data
View Screen	GLC7	AFR1	1	Unpublished Fields Lab Data
View Screen	GLC7	DID4	1	Unpublished Fields Lab Data
View Screen	GLC7	PDE2	1	Unpublished Fields Lab Data
View Screen	GLC7	TPO1	1	Unpublished Fields Lab Data
View Screen	GLC7	DIG1	1	Unpublished Fields Lab Data
View Screen	GLC7	COQ4	1	Unpublished Fields Lab Data
View Screen	GLC7	YLR152C	1	Unpublished Fields Lab Data
View Screen	GLC7	GIP3	1	Unpublished Fields Lab Data
View Screen	GLC7	DCR2	1	Unpublished Fields Lab Data
View Screen	GLC7	YPR076W	1	Unpublished Fields Lab Data
View Screen	GLC7	MRPL28	1	Unpublished Fields Lab Data
View Screen	GLC7	YMR018W	1	Unpublished Fields Lab Data
View Screen	GLC7	SMX3	1	Unpublished Fields Lab Data
View Screen	GLC7	JIP4	1	Unpublished Fields Lab Data
View Screen	GLC7	IRC21	1	Unpublished Fields Lab Data
View Screen	GLC7	GIP2	1	Unpublished Fields Lab Data
View Screen	GLC7	VPS20	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	GLC7	Huh WK, et al. (2003)
View Data	GLC7	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..312]	PSI-BLAST	1734.0	Protein phosphatase-1 (PP-1)

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
phosphoprotein phosphatase activity	11.6417995308386	bayes_pls_golite062009
protein serine/threonine phosphatase activity	10.6440947462013	bayes_pls_golite062009
phosphatase activity	10.5525832502704	bayes_pls_golite062009
phosphoric ester hydrolase activity	10.3702645338723	bayes_pls_golite062009
hydrolase activity, acting on ester bonds	9.02791306892273	bayes_pls_golite062009
calcium-dependent protein serine/threonine phosphatase activity	6.04386346716682	bayes_pls_golite062009
myosin phosphatase activity	5.31703100176823	bayes_pls_golite062009
hydrolase activity	3.13006743434485	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	1.68552051869632	bayes_pls_golite062009
kinase activity	1.67788383575784	bayes_pls_golite062009
structural molecule activity	1.5850868178597	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	1.57807175808088	bayes_pls_golite062009
protein kinase activity	1.4514460578232	bayes_pls_golite062009
binding	1.44538582463569	bayes_pls_golite062009
catalytic activity	1.39852585255405	bayes_pls_golite062009
transferase activity	1.3407391509244	bayes_pls_golite062009
protein binding	1.33435965029646	bayes_pls_golite062009
transporter activity	1.24767072239634	bayes_pls_golite062009
molecular transducer activity	1.08090214431427	bayes_pls_golite062009
signal transducer activity	1.08090214431427	bayes_pls_golite062009
calmodulin binding	0.77173071932406	bayes_pls_golite062009
protein serine/threonine kinase activity	0.66803085638835	bayes_pls_golite062009
substrate-specific transporter activity	0.528189469950839	bayes_pls_golite062009
structural constituent of muscle	0.126411744957937	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2